Triadin Monoclonal Antibody (GE 4.90) | Invitrogen MA3-927 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Triadin Monoclonal Antibody (GE 4.90)

catalog :

MA3-927

quantity :

200µL

price :

US 390

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

GE 4.90

reactivity :

mouse, rat, pigs , domestic rabbit

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 29

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 3a	Lee C, Hanna A, Wang H, Dagnino Acosta A, Joshi A, Knoblauch M, et al. A chemical chaperone improves muscle function in mice with a RyR1 mutation. Nat Commun. 2017;8:14659 pubmed publisher
western blot; domestic rabbit	Desmond P, Muriel J, Markwardt M, Rizzo M, Bloch R. Identification of Small Ankyrin 1 as a Novel Sarco(endo)plasmic Reticulum Ca2+-ATPase 1 (SERCA1) Regulatory Protein in Skeletal Muscle. J Biol Chem. 2015;290:27854-67 pubmed publisher
western blot; mouse	Van B, Nishi M, Komazaki S, Ichimura A, Kakizawa S, Nakanaga K, et al. Mitsugumin 56 (hedgehog acyltransferase-like) is a sarcoplasmic reticulum-resident protein essential for postnatal muscle maturation. FEBS Lett. 2015;589:1095-104 pubmed publisher
immunocytochemistry; rat western blot; rat	Sleiman N, McFarland T, Jones L, Cala S. Transitions of protein traffic from cardiac ER to junctional SR. J Mol Cell Cardiol. 2015;81:34-45 pubmed publisher
western blot; mouse	Boncompagni S, Thomas M, Lopez J, Allen P, Yuan Q, Kranias E, et al. Triadin/Junctin double null mouse reveals a differential role for Triadin and Junctin in anchoring CASQ to the jSR and regulating Ca(2+) homeostasis. PLoS ONE. 2012;7:e39962 pubmed publisher
western blot; rat; 1:1000	Han P, Cai W, Wang Y, Lam C, Arvanitis D, Singh V, et al. Catecholaminergic-induced arrhythmias in failing cardiomyocytes associated with human HRCS96A variant overexpression. Am J Physiol Heart Circ Physiol. 2011;301:H1588-95 pubmed publisher
western blot; mouse	Jeppesen J, Albers P, Rose A, Birk J, Schjerling P, Dzamko N, et al. Contraction-induced skeletal muscle FAT/CD36 trafficking and FA uptake is AMPK independent. J Lipid Res. 2011;52:699-711 pubmed publisher
western blot; mouse; 1:500	Ojima K, Ono Y, Ottenheijm C, Hata S, Suzuki H, Granzier H, et al. Non-proteolytic functions of calpain-3 in sarcoplasmic reticulum in skeletal muscles. J Mol Biol. 2011;407:439-49 pubmed publisher
western blot; mouse	Eltit J, Feng W, Lopez J, Padilla I, Pessah I, Molinski T, et al. Ablation of skeletal muscle triadin impairs FKBP12/RyR1 channel interactions essential for maintaining resting cytoplasmic Ca2+. J Biol Chem. 2010;285:38453-62 pubmed publisher
western blot; mouse	Rizzi N, Liu N, Napolitano C, Nori A, Turcato F, Colombi B, et al. Unexpected structural and functional consequences of the R33Q homozygous mutation in cardiac calsequestrin: a complex arrhythmogenic cascade in a knock in mouse model. Circ Res. 2008;103:298-306 pubmed publisher
immunoprecipitation; domestic rabbit; 1:1000; fig 8	Phimister A, Lango J, Lee E, Ernst Russell M, Takeshima H, Ma J, et al. Conformation-dependent stability of junctophilin 1 (JP1) and ryanodine receptor type 1 (RyR1) channel complex is mediated by their hyper-reactive thiols. J Biol Chem. 2007;282:8667-77 pubmed
western blot; mouse; 1:1,000	Jaehnig E, Heidt A, Greene S, Cornelissen I, Black B. Increased susceptibility to isoproterenol-induced cardiac hypertrophy and impaired weight gain in mice lacking the histidine-rich calcium-binding protein. Mol Cell Biol. 2006;26:9315-26 pubmed
western blot; pigs	Schulz J, Palmer N, Steckelberg J, Jones S, Zeece M. Microarray profiling of skeletal muscle sarcoplasmic reticulum proteins. Biochim Biophys Acta. 2006;1764:1429-35 pubmed
western blot; mouse	Perez C, Lopez J, Allen P. Expression levels of RyR1 and RyR3 control resting free Ca2+ in skeletal muscle. Am J Physiol Cell Physiol. 2005;288:C640-9 pubmed
	Estañ M, Fernández Núñez E, Zaki M, Esteban M, Donkervoort S, Hawkins C, et al. Recessive mutations in muscle-specific isoforms of FXR1 cause congenital multi-minicore myopathy. Nat Commun. 2019;10:797 pubmed publisher
	Cerrone M, Montnach J, Lin X, Zhao Y, Zhang M, Agullo Pascual E, et al. Plakophilin-2 is required for transcription of genes that control calcium cycling and cardiac rhythm. Nat Commun. 2017;8:106 pubmed publisher
	Eltit J, Szpyt J, Li H, Allen P, Perez C. Reduced gain of excitation-contraction coupling in triadin-null myotubes is mediated by the disruption of FKBP12/RyR1 interaction. Cell Calcium. 2011;49:128-35 pubmed publisher
	Shen X, Franzini Armstrong C, Lopez J, Jones L, Kobayashi Y, Wang Y, et al. Triadins modulate intracellular Ca(2+) homeostasis but are not essential for excitation-contraction coupling in skeletal muscle. J Biol Chem. 2007;282:37864-74 pubmed
	Rose A, Alsted T, Kobberø J, Richter E. Regulation and function of Ca2+-calmodulin-dependent protein kinase II of fast-twitch rat skeletal muscle. J Physiol. 2007;580:993-1005 pubmed
	Hirata Y, Nakahata N, Ohizumi Y. Identification of a 97-kDa mastoparan-binding protein involving in Ca(2+) release from skeletal muscle sarcoplasmic reticulum. Mol Pharmacol. 2000;57:1235-42 pubmed
	Sato Y, Ferguson D, Sako H, Dorn G, Kadambi V, Yatani A, et al. Cardiac-specific overexpression of mouse cardiac calsequestrin is associated with depressed cardiovascular function and hypertrophy in transgenic mice. J Biol Chem. 1998;273:28470-7 pubmed
	Protasi F, Franzini Armstrong C, Allen P. Role of ryanodine receptors in the assembly of calcium release units in skeletal muscle. J Cell Biol. 1998;140:831-42 pubmed
	Flucher B, Andrews S, Fleischer S, Marks A, Caswell A, Powell J. Triad formation: organization and function of the sarcoplasmic reticulum calcium release channel and triadin in normal and dysgenic muscle in vitro. J Cell Biol. 1993;123:1161-74 pubmed
	Motoike H, Caswell A, Smilowitz H, Brandt N. Extraction of junctional complexes from triad junctions of rabbit skeletal muscle. J Muscle Res Cell Motil. 1994;15:493-504 pubmed
	Liu G, Pessah I. Molecular interaction between ryanodine receptor and glycoprotein triadin involves redox cycling of functionally important hyperreactive sulfhydryls. J Biol Chem. 1994;269:33028-34 pubmed
	Brandt N, Caswell A, Carl S, Ferguson D, Brandt T, Brunschwig J, et al. Detection and localization of triadin in rat ventricular muscle. J Membr Biol. 1993;131:219-28 pubmed
	Fan H, Brandt N, Peng M, Schwartz A, Caswell A. Binding sites of monoclonal antibodies and dihydropyridine receptor alpha 1 subunit cytoplasmic II-III loop on skeletal muscle triadin fusion peptides. Biochemistry. 1995;34:14893-901 pubmed
	Caswell A, Brandt N, Brunschwig J, Purkerson S. Localization and partial characterization of the oligomeric disulfide-linked molecular weight 95,000 protein (triadin) which binds the ryanodine and dihydropyridine receptors in skeletal muscle triadic vesicles. Biochemistry. 1991;30:7507-13 pubmed
	Brandt N, Caswell A, Brunschwig J, Kang J, Antoniu B, Ikemoto N. Effects of anti-triadin antibody on Ca2+ release from sarcoplasmic reticulum. FEBS Lett. 1992;299:57-9 pubmed

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product information

Product Type :

Antibody

Product Name :

Triadin Monoclonal Antibody (GE 4.90)

Catalog # :

MA3-927

Quantity :

200µL

Price :

US 390

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Mouse, Rabbit, Rat

Applications :

ELISA: Assay dependent, Immunofluorescence: Assay-dependent, Immunohistochemistry (Frozen): 1:50, Immunoprecipitation: Assay dependent, Western Blot: 1:500

Species :

Mouse, Rabbit, Rat

Clone :

GE 4.90

Isotype :

IgG

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPR’s) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyR’s), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, triadin (MW 95 kDa) transmits electrochemical signals to the SR through direct interaction with both DHPR’s and RyR’s. Though its exact role in this signaling process is unclear, triadin has been shown to co-localize with both DHPR and RYR at the junctional face of the terminal cisternae.

Immunogen :

Purified rabbit fast skeletal muscle triadin.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay dependent, Immunofluorescence: Assay-dependent, Immunohistochemistry (Frozen): 1:50, Immunoprecipitation: Assay dependent, Western Blot: 1:500

Aliases :

2310045H21Rik; cardiac triadin; CPVT5; EG432451; junctional sarcoplasmic reticulum glycoprotein; TDN; Trdn; Triadin; triadin 1; triadin 32 kDa (TRISK 32); triadin 49 kDa (TRISK 49); triadin 95; TRISK

more info or order :

Invitrogen product webpage