ATP4B Monoclonal Antibody (2G11) | Invitrogen MA3-923 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

ATP4B Monoclonal Antibody (2G11)

catalog :

MA3-923

quantity :

100 µL

price :

US 412.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2G11

reactivity :

groundhog, guinea pig, human, mouse, rat, dogs, bovine, pigs , domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 59

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:2000; loading ...; fig 5b	Kurata A, Takanashi M, Ohno S, Fujita K, Kuroda M. Cisplatin induces differentiation in teratomas derived from pluripotent stem cells. Regen Ther. 2021;18:117-126 pubmed publisher
immunohistochemistry - paraffin section; human; 1:2000; loading ...; fig 1e	Wilhelm F, Böger C, Krüger S, Behrens H, Rocken C. Troy is expressed in human stomach mucosa and a novel putative prognostic marker of intestinal type gastric cancer. Oncotarget. 2017;8:50557-50569 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:8000; tbl 1	Fossmark R, Rao S, Mjønes P, Munkvold B, Flatberg A, Varro A, et al. PAI-1 deficiency increases the trophic effects of hypergastrinemia in the gastric corpus mucosa. Peptides. 2016;79:83-94 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000; fig s2	Xuan S, Sussel L. GATA4 and GATA6 regulate pancreatic endoderm identity through inhibition of hedgehog signaling. Development. 2016;143:780-6 pubmed publisher
immunohistochemistry - paraffin section; human; 1:2000	Shah V, Meyerholz D, Tang X, Reznikov L, Abou Alaiwa M, Ernst S, et al. Airway acidification initiates host defense abnormalities in cystic fibrosis mice. Science. 2016;351:503-7 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:1000 flow cytometry; mouse; 1:100	Schumacher M, Aihara E, Feng R, Engevik A, Shroyer N, Ottemann K, et al. The use of murine-derived fundic organoids in studies of gastric physiology. J Physiol. 2015;593:1809-27 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:1000 immunocytochemistry; mouse; 1:1000	Schumacher M, Feng R, Aihara E, Engevik A, Montrose M, Ottemann K, et al. Helicobacter pylori-induced Sonic Hedgehog expression is regulated by NFÎºB pathway activation: the use of a novel in vitro model to study epithelial response to infection. Helicobacter. 2015;20:19-28 pubmed publisher
immunocytochemistry; domestic rabbit; 1:1000	Natarajan P, Crothers J, Rosen J, Nakada S, Rakholia M, Okamoto C, et al. Myosin IIB and F-actin control apical vacuolar morphology and histamine-induced trafficking of H-K-ATPase-containing tubulovesicles in gastric parietal cells. Am J Physiol Gastrointest Liver Physiol. 2014;306:G699-710 pubmed publisher
immunohistochemistry; groundhog; 1:4000	Sørdal Ø, Waldum H, Nordrum I, Boyce M, Bergh K, Munkvold B, et al. The gastrin receptor antagonist netazepide (YF476) prevents oxyntic mucosal inflammation induced by Helicobacter pylori infection in Mongolian gerbils. Helicobacter. 2013;18:397-405 pubmed publisher
immunohistochemistry; domestic rabbit; 1:50 western blot; domestic rabbit; 1:5000	He W, Liu W, Chew C, Baker S, Baker R, Forte J, et al. Acid secretion-associated translocation of KCNJ15 in gastric parietal cells. Am J Physiol Gastrointest Liver Physiol. 2011;301:G591-600 pubmed publisher
immunohistochemistry; rat; 1:2000	Gustafsson B, Bakke I, Hauso Ø, Kidd M, Modlin I, Fossmark R, et al. Parietal cell activation by arborization of ECL cell cytoplasmic projections is likely the mechanism for histamine induced secretion of hydrochloric acid. Scand J Gastroenterol. 2011;46:531-7 pubmed publisher
immunohistochemistry; mouse; 0.5 mg/ml	Roepke T, King E, Purtell K, Kanda V, Lerner D, Abbott G. Genetic dissection reveals unexpected influence of beta subunits on KCNQ1 K+ channel polarized trafficking in vivo. FASEB J. 2011;25:727-36 pubmed publisher
immunohistochemistry; mouse	Okumura T, Ericksen R, Takaishi S, Wang S, Dubeykovskiy Z, Shibata W, et al. K-ras mutation targeted to gastric tissue progenitor cells results in chronic inflammation, an altered microenvironment, and progression to intraepithelial neoplasia. Cancer Res. 2010;70:8435-45 pubmed publisher
immunocytochemistry; domestic rabbit	Zhu L, Crothers J, Zhou R, Forte J. A possible mechanism for ezrin to establish epithelial cell polarity. Am J Physiol Cell Physiol. 2010;299:C431-43 pubmed publisher
immunohistochemistry; mouse	Udd L, Katajisto P, Kyyrönen M, Ristimaki A, Makela T. Impaired gastric gland differentiation in Peutz-Jeghers syndrome. Am J Pathol. 2010;176:2467-76 pubmed publisher
immunohistochemistry; mouse; 1:10000	Alaynick W, Way J, Wilson S, Benson W, Pei L, Downes M, et al. ERRgamma regulates cardiac, gastric, and renal potassium homeostasis. Mol Endocrinol. 2010;24:299-309 pubmed publisher
immunohistochemistry; mouse; 1:1000	Xiao C, Ogle S, Schumacher M, Orr Asman M, Miller M, Lertkowit N, et al. Loss of parietal cell expression of Sonic hedgehog induces hypergastrinemia and hyperproliferation of surface mucous cells. Gastroenterology. 2010;138:550-61, 561.e1-8 pubmed publisher
immunohistochemistry; mouse; 1:2000	Rotte A, Mack A, Bhandaru M, Kempe D, Beier N, Scholz W, et al. Pioglitazone induced gastric acid secretion. Cell Physiol Biochem. 2009;24:193-200 pubmed publisher
immunohistochemistry; human; 1:1,000	Haveri H, Westerholm Ormio M, Lindfors K, Maki M, Savilahti E, Andersson L, et al. Transcription factors GATA-4 and GATA-6 in normal and neoplastic human gastrointestinal mucosa. BMC Gastroenterol. 2008;8:9 pubmed publisher
immunocytochemistry; mouse; 1:2000	El Zaatari M, Grabowska A, McKenzie A, Powe D, Scotting P, Watson S. Cyclopamine inhibition of the sonic hedgehog pathway in the stomach requires concomitant acid inhibition. Regul Pept. 2008;146:131-9 pubmed
immunocytochemistry; human immunohistochemistry; human; 1:2000	Hauso Ø, Gustafsson B, Waldum H. Long slender cytoplasmic extensions: a common feature of neuroendocrine cells?. J Neuroendocrinol. 2007;19:739-42 pubmed
western blot; domestic rabbit; 1:4	Mettler S, Ghayouri S, Christensen G, Forte J. Modulatory role of phosphoinositide 3-kinase in gastric acid secretion. Am J Physiol Gastrointest Liver Physiol. 2007;293:G532-43 pubmed
immunohistochemistry; guinea pig	Schreiber S, Garten D, Nguyen T, Konradt M, Bücker R, Scheid P. In situ measurement of pH in the secreting canaliculus of the gastric parietal cell and adjacent structures. Cell Tissue Res. 2007;329:313-20 pubmed
immunohistochemistry; mouse	Saegusa C, Tanaka T, Tani S, Itohara S, Mikoshiba K, Fukuda M. Decreased basal mucus secretion by Slp2-a-deficient gastric surface mucous cells. Genes Cells. 2006;11:623-31 pubmed
immunohistochemistry; human	Bakkelund K, Fossmark R, Nordrum I, Waldum H. Signet ring cells in gastric carcinomas are derived from neuroendocrine cells. J Histochem Cytochem. 2006;54:615-21 pubmed
immunohistochemistry; mouse; 1:500	Takamoto N, You L, Moses K, Chiang C, Zimmer W, Schwartz R, et al. COUP-TFII is essential for radial and anteroposterior patterning of the stomach. Development. 2005;132:2179-89 pubmed
	Sokolova E, Shilova O, Kiseleva D, Schulga A, Balalaeva I, Deyev S. HER2-Specific Targeted Toxin DARPin-LoPE: Immunogenicity and Antitumor Effect on Intraperitoneal Ovarian Cancer Xenograft Model. Int J Mol Sci. 2019;20: pubmed publisher
	Janardhan K, Jensen H, Clayton N, Herbert R. Immunohistochemistry in Investigative and Toxicologic Pathology. Toxicol Pathol. 2018;46:488-510 pubmed publisher
	Vange P, Bruland T, Doseth B, Fossmark R, Sousa M, Beisvag V, et al. The cytoprotective protein clusterin is overexpressed in hypergastrinemic rodent models of oxyntic preneoplasia and promotes gastric cancer cell survival. PLoS ONE. 2017;12:e0184514 pubmed publisher
	Engevik A, Feng R, Choi E, White S, Bertaux Skeirik N, Li J, et al. The Development of Spasmolytic Polypeptide/TFF2-Expressing Metaplasia (SPEM) During Gastric Repair Is Absent in the Aged Stomach. Cell Mol Gastroenterol Hepatol. 2016;2:605-624 pubmed
	Bertaux Skeirik N, Feng R, Schumacher M, Li J, Mahé M, Engevik A, et al. CD44 plays a functional role in Helicobacter pylori-induced epithelial cell proliferation. PLoS Pathog. 2015;11:e1004663 pubmed publisher
	McCracken K, CatÃ¡ E, Crawford C, Sinagoga K, Schumacher M, Rockich B, et al. Modelling human development and disease in pluripotent stem-cell-derived gastric organoids. Nature. 2014;516:400-4 pubmed publisher
	Sidani S, Kirchhoff P, Socrates T, Stelter L, Ferreira E, Caputo C, et al. DeltaF508 mutation results in impaired gastric acid secretion. J Biol Chem. 2007;282:6068-74 pubmed
	Roepke T, Anantharam A, Kirchhoff P, Busque S, Young J, Geibel J, et al. The KCNE2 potassium channel ancillary subunit is essential for gastric acid secretion. J Biol Chem. 2006;281:23740-7 pubmed
	Tamura A, Kikuchi S, Hata M, Katsuno T, Matsui T, Hayashi H, et al. Achlorhydria by ezrin knockdown: defects in the formation/expansion of apical canaliculi in gastric parietal cells. J Cell Biol. 2005;169:21-8 pubmed
	Cui G, Koh T, Chen D, Zhao C, Takaishi S, Dockray G, et al. Overexpression of glycine-extended gastrin inhibits parietal cell loss and atrophy in the mouse stomach. Cancer Res. 2004;64:8160-6 pubmed
	Ganley I, Carroll K, Bittova L, Pfeffer S. Rab9 GTPase regulates late endosome size and requires effector interaction for its stability. Mol Biol Cell. 2004;15:5420-30 pubmed
	De Block C, Colpin G, Thielemans K, Coopmans W, Bogers J, Pelckmans P, et al. Neuroendocrine tumor markers and enterochromaffin-like cell hyper/dysplasia in type 1 diabetes. Diabetes Care. 2004;27:1387-93 pubmed
	Fossmark R, Martinsen T, Bakkelund K, Kawase S, Waldum H. ECL-cell derived gastric cancer in male cotton rats dosed with the H2-blocker loxtidine. Cancer Res. 2004;64:3687-93 pubmed
	Yip L, Leung H, Kwok Y. Role of adenosine A1 receptor in the regulation of gastrin release. J Pharmacol Exp Ther. 2004;310:477-87 pubmed
	Pestov N, Korneenko T, Radkov R, Zhao H, Shakhparonov M, Modyanov N. Identification of the beta-subunit for nongastric H-K-ATPase in rat anterior prostate. Am J Physiol Cell Physiol. 2004;286:C1229-37 pubmed
	Yip L, Kwok Y. Role of adenosine A2A receptor in the regulation of gastric somatostatin release. J Pharmacol Exp Ther. 2004;309:804-15 pubmed
	Sawaguchi A, McDonald K, Forte J. High-pressure freezing of isolated gastric glands provides new insight into the fine structure and subcellular localization of H+/K+-ATPase in gastric parietal cells. J Histochem Cytochem. 2004;52:77-86 pubmed
	Beisvag V, Falck G, Loennechen J, Qvigstad G, Jynge P, Skomedal T, et al. Identification and regulation of the gastric H+/K+-ATPase in the rat heart. Acta Physiol Scand. 2003;179:251-62 pubmed
	Kirchhoff P, Wagner C, Gaetzschmann F, Radebold K, Geibel J. Demonstration of a functional apical sodium hydrogen exchanger in isolated rat gastric glands. Am J Physiol Gastrointest Liver Physiol. 2003;285:G1242-8 pubmed
	Zhou R, Watson C, Fu C, Yao X, Forte J. Myosin II is present in gastric parietal cells and required for lamellipodial dynamics associated with cell activation. Am J Physiol Cell Physiol. 2003;285:C662-73 pubmed
	De Block C, De Leeuw I, Bogers J, Pelckmans P, Ieven M, Van Marck E, et al. Autoimmune gastropathy in type 1 diabetic patients with parietal cell antibodies: histological and clinical findings. Diabetes Care. 2003;26:82-8 pubmed
	Karvar S, Yao X, Crothers J, Liu Y, Forte J. Localization and function of soluble N-ethylmaleimide-sensitive factor attachment protein-25 and vesicle-associated membrane protein-2 in functioning gastric parietal cells. J Biol Chem. 2002;277:50030-5 pubmed
	van den Brink G, Hardwick J, Nielsen C, Xu C, ten Kate F, Glickman J, et al. Sonic hedgehog expression correlates with fundic gland differentiation in the adult gastrointestinal tract. Gut. 2002;51:628-33 pubmed
	Sandvik A, Cui G, Bakke I, Munkvold B, Waldum H. PACAP stimulates gastric acid secretion in the rat by inducing histamine release. Am J Physiol Gastrointest Liver Physiol. 2001;281:G997-G1003 pubmed
	Ammar D, Nguyen P, Forte J. Functionally distinct pools of actin in secretory cells. Am J Physiol Cell Physiol. 2001;281:C407-17 pubmed
	Adams G, Tillekeratne M, Yu C, Pestov N, Modyanov N. Catalytic function of nongastric H,K-ATPase expressed in Sf-21 insect cells. Biochemistry. 2001;40:5765-76 pubmed
	Okamoto C, Duman J, Tyagarajan K, McDonald K, Jeng Y, McKinney J, et al. Clathrin in gastric acid secretory (parietal) cells: biochemical characterization and subcellular localization. Am J Physiol Cell Physiol. 2000;279:C833-51 pubmed
	Bakke I, Sandvik A, Waldum H. Octreotide inhibits the enterochromaffin-like cell but not peroxisome proliferator-induced hypergastrinemia. J Mol Endocrinol. 2000;25:109-19 pubmed
	Bakke I, Qvigstad G, Brenna E, Sandvik A, Waldum H. Gastrin has a specific proliferative effect on the rat enterochromaffin-like cell, but not on the parietal cell: a study by elutriation centrifugation. Acta Physiol Scand. 2000;169:29-37 pubmed
	Duman J, Tyagarajan K, Kolsi M, Moore H, Forte J. Expression of rab11a N124I in gastric parietal cells inhibits stimulatory recruitment of the H+-K+-ATPase. Am J Physiol. 1999;277:C361-72 pubmed publisher
	Levine D, Iacovitti M, Buckman S, Luck B, Hincke M, Burns K, et al. K depletion stimulates in vivo HCO3 reabsorption in surviving rat distal tubules. Am J Physiol. 1998;274:F665-72 pubmed publisher
	Chow D, Forte J. Characterization of the beta-subunit of the H(+)-K(+)-ATPase using an inhibitory monoclonal antibody. Am J Physiol. 1993;265:C1562-70 pubmed
	Dintzis S, Velculescu V, Pfeffer S. Receptor extracellular domains may contain trafficking information. Studies of the 300-kDa mannose 6-phosphate receptor. J Biol Chem. 1994;269:12159-66 pubmed

image

image 1 :

Immunofluorescent analysis of Hydrogen/Potassium ATPase beta using Anti-Hydrogen/Potassium ATPase beta Monoclonal Antibody (2G11) (Product# MA3-923) shows staining in Hela Cells. Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Hydrogen/Potassium ATPase beta (Product# MA3-923) at a dilution of 1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

ATP4B Monoclonal Antibody (2G11)

Catalog # :

MA3-923

Quantity :

100 µL

Price :

US 412.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Bovine, Canine, Human, Mouse, Mustelid, Porcine, Rabbit, Rat

Applications :

ChIP assay: Assay-dependent, Flow Cytometry: 1/50, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Frozen): 1:2,000, Immunohistochemistry (Paraffin): 1:2,000, Immunoprecipitation: Assay-dependent, Western Blot: 1:4,000

Species :

Bovine, Canine, Human, Mouse, Mustelid, Porcine, Rabbit, Rat

Clone :

2G11

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The hydrogen/potassium ATPase, or gastric proton pump, belongs to a family of P-type cation-transporting ATPases. This family of ATPases shares a number of functional and structural similarities including the common feature of consisting of an alpha and beta subunit. Like the ubiquitous sodium/potassium ATPase, the hydrogen/potassium ATPase consists of a large transmembrane catalytic subunit, termed the alpha- subunit which contains sites for ATP binding and phosphorylation, and an associated smaller glycoprotein, termed the beta-subunit which may play a role in maintaining the structural and functional integrity of the complex.

Immunogen :

Purified 34 kDa core peptide from deglycosylated hog gastric microsomes.

Format :

Liquid

Applications w/Dilutions :

Aliases :

(H+,K+)-ATPase beta-subunit; ATP4B; ATP6B; ATPase H+/K+ transporting beta subunit; ATPase H+/K+ transporting subunit beta; ATPase H+K+-transporting beta / (gastric HK-ATPase beta subunit) defined by SSR; ATPase, H+,K+-transporting, beta / (gastric H,K-ATPase beta subunit), defined by SSR; ATPase, H+/K+ exchanging, beta polypeptide; ATPase, H+/K+ transporting, beta polypeptide; ATPase, H+/K+ transporting, beta polypeptide, gastric specific; AV080843; gastric H(+)/K(+) ATPase subunit beta; gastric H+/K+ ATPase beta subunit; gastric H+/K+-ATPase beta subunit; gastric hydrogen-potassium ATPase, beta; gp60-90; H,K-ATPase-Beta; H.K-ATPase, beta subunit; H+,K+-ATPase; H+/K+ ATPase beta; H+/K+ ATPase beta subunit; H+/K+-ATPase beta; H+/K+-ATPase beta subunit; potassium-transporting ATPase beta chain; potassium-transporting ATPase subunit beta; Proton pump beta chain; S76401S1

more info or order :

Invitrogen product webpage