product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Phospholamban Monoclonal Antibody (2D12)
catalog :
MA3-922
quantity :
100 µg
price :
US 399.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2D12
reactivity :
guinea pig, human, mouse, rat, dogs, chicken, pigs , domestic sheep, domestic rabbit
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 151
Published Application/Species/Sample/DilutionReference
  • western blot; domestic rabbit; 1:2000; loading ...; fig 5
Chang S, Chuang H, Chen Y, Kao Y, Lin Y, Yeh Y, et al. Heart failure modulates electropharmacological characteristics of sinoatrial nodes. Exp Ther Med. 2017;13:771-779 pubmed publisher
  • western blot; mouse; loading ...; fig 1b
Fajardo V, Gamu D, Mitchell A, Bloemberg D, Bombardier E, Chambers P, et al. Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice. PLoS ONE. 2017;12:e0173708 pubmed publisher
  • western blot; rat; fig 8b
Kirschmer N, Bandleon S, von Ehrlich Treuenstätt V, Hartmann S, Schaaf A, Lamprecht A, et al. TRPC4? and TRPC4? Similarly Affect Neonatal Cardiomyocyte Survival during Chronic GPCR Stimulation. PLoS ONE. 2016;11:e0168446 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2c
Fu Q, Hu Y, Wang Q, Liu Y, Li N, Xu B, et al. High-fat diet induces protein kinase A and G-protein receptor kinase phosphorylation of β2 -adrenergic receptor and impairs cardiac adrenergic reserve in animal hearts. J Physiol. 2017;595:1973-1986 pubmed publisher
  • western blot; mouse; 1:2000; fig 1
Fajardo V, Smith I, Bombardier E, Chambers P, Quadrilatero J, Tupling A. Diaphragm assessment in mice overexpressing phospholamban in slow-twitch type I muscle fibers. Brain Behav. 2016;6:e00470 pubmed publisher
  • western blot; rat; 1:5000; fig s1
Sadredini M, Danielsen T, Aronsen J, Manotheepan R, Hougen K, Sjaastad I, et al. Beta-Adrenoceptor Stimulation Reveals Ca2+ Waves and Sarcoplasmic Reticulum Ca2+ Depletion in Left Ventricular Cardiomyocytes from Post-Infarction Rats with and without Heart Failure. PLoS ONE. 2016;11:e0153887 pubmed publisher
  • western blot; mouse; 1:5000; loading ...; fig s9a
Nelson B, Makarewich C, Anderson D, Winders B, Troupes C, Wu F, et al. A peptide encoded by a transcript annotated as long noncoding RNA enhances SERCA activity in muscle. Science. 2016;351:271-5 pubmed publisher
  • western blot; human
  • western blot; mouse
Fajardo V, Bombardier E, McMillan E, TRAN K, Wadsworth B, Gamu D, et al. Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype. Dis Model Mech. 2015;8:999-1009 pubmed publisher
  • western blot; rat; fig 5
Dalpiaz P, Lamas A, Caliman I, Ribeiro R, Abreu G, Moyses M, et al. Sex Hormones Promote Opposite Effects on ACE and ACE2 Activity, Hypertrophy and Cardiac Contractility in Spontaneously Hypertensive Rats. PLoS ONE. 2015;10:e0127515 pubmed publisher
  • western blot; mouse; 1:20000
Major J, Salih M, Tuana B. Interplay between the E2F pathway and β-adrenergic signaling in the pathological hypertrophic response of myocardium. J Mol Cell Cardiol. 2015;84:179-90 pubmed publisher
  • western blot; domestic rabbit
Moshal K, Zhang Z, Roder K, Kim T, Cooper L, Patedakis Litvinov B, et al. Progesterone modulates SERCA2a expression and function in rabbit cardiomyocytes. Am J Physiol Cell Physiol. 2014;307:C1050-7 pubmed publisher
  • western blot; rat; 1:5000; fig 5
Freire P, Alves C, Deus A, Leopoldo A, Leopoldo A, Silva D, et al. Obesity does not lead to imbalance between myocardial phospholamban phosphorylation and dephosphorylation. Arq Bras Cardiol. 2014;103:41-50 pubmed
  • western blot; mouse
Holemans T, Vandecaetsbeek I, Wuytack F, Vangheluwe P. Measuring Ca2+-dependent Ca2+-uptake activity in the mouse heart. Cold Spring Harb Protoc. 2014;2014:876-86 pubmed publisher
  • western blot; rat; 1:1000
Fioresi M, Simões M, Furieri L, Broseghini Filho G, Vescovi M, Stefanon I, et al. Chronic lead exposure increases blood pressure and myocardial contractility in rats. PLoS ONE. 2014;9:e96900 pubmed publisher
  • western blot; rat; 1:1000
Faria T, Costa G, Almenara C, Angeli J, Vassallo D, Stefanon I, et al. Chronic exposure to low doses of HgCl2 avoids calcium handling impairment in the right ventricle after myocardial infarction in rats. PLoS ONE. 2014;9:e95639 pubmed publisher
  • western blot; rat; 1:1000
Mourouzis I, Mantzouratou P, Galanopoulos G, Kostakou E, Dhalla A, Belardinelli L, et al. The beneficial effects of ranolazine on cardiac function after myocardial infarction are greater in diabetic than in nondiabetic rats. J Cardiovasc Pharmacol Ther. 2014;19:457-69 pubmed publisher
  • western blot; human; 1:2000
Sivagangabalan G, Nazzari H, Bignolais O, Maguy A, Naud P, Farid T, et al. Regional ion channel gene expression heterogeneity and ventricular fibrillation dynamics in human hearts. PLoS ONE. 2014;9:e82179 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:250; fig 4
  • immunoprecipitation; human; fig 6
  • western blot; human; 1:2000; fig 3
Fajardo V, Bombardier E, Vigna C, Devji T, Bloemberg D, Gamu D, et al. Co-expression of SERCA isoforms, phospholamban and sarcolipin in human skeletal muscle fibers. PLoS ONE. 2013;8:e84304 pubmed publisher
  • western blot; mouse; 1:10000
Haddad R, Kasneci A, Sebag I, Chalifour L. Cardiac structure/function, protein expression, and DNA methylation are changed in adult female mice exposed to diethylstilbestrol in utero. Can J Physiol Pharmacol. 2013;91:741-9 pubmed publisher
  • western blot; rat
Yi T, Vick J, Vecchio M, Begin K, Bell S, Delay R, et al. Identifying cellular mechanisms of zinc-induced relaxation in isolated cardiomyocytes. Am J Physiol Heart Circ Physiol. 2013;305:H706-15 pubmed publisher
  • western blot; mouse; 1:1000
Mourouzis I, Kostakou E, Galanopoulos G, Mantzouratou P, Pantos C. Inhibition of thyroid hormone receptor ?1 impairs post-ischemic cardiac performance after myocardial infarction in mice. Mol Cell Biochem. 2013;379:97-105 pubmed publisher
  • western blot; mouse; 1:10000
Patel B, Raad M, Sebag I, Chalifour L. Lifelong exposure to bisphenol a alters cardiac structure/function, protein expression, and DNA methylation in adult mice. Toxicol Sci. 2013;133:174-85 pubmed publisher
  • western blot; rat; 0.5 ug/ml
Ribeiro R, Potratz F, Pavan B, Forechi L, Lima F, Fiorim J, et al. Carvedilol prevents ovariectomy-induced myocardial contractile dysfunction in female rat. PLoS ONE. 2013;8:e53226 pubmed publisher
  • western blot; rat; 1:10000
Calvé A, Noiles W, Sebag I, Chalifour L. The impact of doxorubicin and dexrazoxane injection of prepubertal female rats on pregnancy outcome and cardiac function postpartum. Can J Physiol Pharmacol. 2012;90:1527-34 pubmed publisher
  • western blot; mouse; 1:10000
Haddad R, Kasneci A, Mepham K, Sebag I, Chalifour L. Gestational exposure to diethylstilbestrol alters cardiac structure/function, protein expression and DNA methylation in adult male mice progeny. Toxicol Appl Pharmacol. 2013;266:27-37 pubmed publisher
  • western blot; pigs ; 1:3000
Klug D, Boulé S, Wissocque L, Montaigne D, Marechal X, Hassoun S, et al. Right ventricular pacing with mechanical dyssynchrony causes apoptosis interruptus and calcium mishandling. Can J Cardiol. 2013;29:510-8 pubmed publisher
  • western blot; pigs
Cutler M, Wan X, Plummer B, Liu H, Deschenes I, Laurita K, et al. Targeted sarcoplasmic reticulum Ca2+ ATPase 2a gene delivery to restore electrical stability in the failing heart. Circulation. 2012;126:2095-104 pubmed publisher
  • western blot; human
Middlekauff H, Vigna C, Verity M, Fonarow G, Horwich T, Hamilton M, et al. Abnormalities of calcium handling proteins in skeletal muscle mirror those of the heart in humans with heart failure: a shared mechanism?. J Card Fail. 2012;18:724-33 pubmed publisher
  • western blot; rat; 1:3000
ZHANG Y, Tang Y, Zhang M, Chen J, Zhou Q, Sun Y, et al. Fosinopril attenuates the doxorubicin-induced cardiomyopathy by restoring the function of sarcoplasmic reticulum. Cell Biochem Biophys. 2012;64:205-11 pubmed publisher
  • western blot; rat; 1:1000
Calvé A, Haddad R, Barama S, Meilleur M, Sebag I, Chalifour L. Cardiac response to doxorubicin and dexrazoxane in intact and ovariectomized young female rats at rest and after swim training. Am J Physiol Heart Circ Physiol. 2012;302:H2048-57 pubmed publisher
  • western blot; domestic rabbit
Odening K, Choi B, Liu G, Hartmann K, Ziv O, Chaves L, et al. Estradiol promotes sudden cardiac death in transgenic long QT type 2 rabbits while progesterone is protective. Heart Rhythm. 2012;9:823-32 pubmed publisher
  • western blot; mouse
Sjåland C, Lunde P, Swift F, Munkvik M, Ericsson M, Lunde M, et al. Slowed relaxation and preserved maximal force in soleus muscles of mice with targeted disruption of the Serca2 gene in skeletal muscle. J Physiol. 2011;589:6139-55 pubmed publisher
  • western blot; rat; 1:500
Mostarda C, Moraes Silva I, Moreira E, Medeiros A, Piratello A, Consolim Colombo F, et al. Baroreflex sensitivity impairment is associated with cardiac diastolic dysfunction in rats. J Card Fail. 2011;17:519-25 pubmed publisher
  • western blot; rat; 1 ug/ml
Paigel A, Ribeiro R, Fernandes A, Targueta G, Vassallo D, Stefanon I. Myocardial contractility is preserved early but reduced late after ovariectomy in young female rats. Reprod Biol Endocrinol. 2011;9:54 pubmed publisher
  • immunohistochemistry; mouse; 1:500
  • western blot; mouse; 1:500
Rodrigues B, Rosa K, Medeiros A, Schaan B, Brum P, de Angelis K, et al. Hyperglycemia can delay left ventricular dysfunction but not autonomic damage after myocardial infarction in rodents. Cardiovasc Diabetol. 2011;10:26 pubmed publisher
  • western blot; mouse; 1:500
Ferreira J, Moreira J, Campos J, Pereira M, Mattos K, Coelho M, et al. Angiotensin receptor blockade improves the net balance of cardiac Ca(2+) handling-related proteins in sympathetic hyperactivity-induced heart failure. Life Sci. 2011;88:578-85 pubmed publisher
  • western blot; mouse; 1:500
Horiuchi Hirose M, Kashihara T, Nakada T, Kurebayashi N, Shimojo H, Shibazaki T, et al. Decrease in the density of t-tubular L-type Ca2+ channel currents in failing ventricular myocytes. Am J Physiol Heart Circ Physiol. 2011;300:H978-88 pubmed publisher
  • western blot; human; 1:2500
Hamdani N, Borbely A, Veenstra S, Kooij V, Vrydag W, Zaremba R, et al. More severe cellular phenotype in human idiopathic dilated cardiomyopathy compared to ischemic heart disease. J Muscle Res Cell Motil. 2010;31:289-301 pubmed publisher
  • western blot; mouse; 1:1000
van Oort R, McCauley M, Dixit S, Pereira L, Yang Y, Respress J, et al. Ryanodine receptor phosphorylation by calcium/calmodulin-dependent protein kinase II promotes life-threatening ventricular arrhythmias in mice with heart failure. Circulation. 2010;122:2669-79 pubmed publisher
  • immunocytochemistry; rat; 1:2000
  • western blot; rat; 1:2000
Prasad A, Inesi G. Silencing calcineurin A subunit reduces SERCA2 expression in cardiac myocytes. Am J Physiol Heart Circ Physiol. 2011;300:H173-80 pubmed publisher
  • western blot; mouse
Yang L, Kockskamper J, Khan S, Suarez J, Walther S, Doleschal B, et al. cAMP- and Ca²(+) /calmodulin-dependent protein kinases mediate inotropic, lusitropic and arrhythmogenic effects of urocortin 2 in mouse ventricular myocytes. Br J Pharmacol. 2011;162:544-56 pubmed publisher
  • western blot; mouse; 1:1000
Alcalai R, Wakimoto H, Arad M, Planer D, Konno T, Wang L, et al. Prevention of ventricular arrhythmia and calcium dysregulation in a catecholaminergic polymorphic ventricular tachycardia mouse model carrying calsequestrin-2 mutation. J Cardiovasc Electrophysiol. 2011;22:316-24 pubmed publisher
  • western blot; rat
Sun L, Ai J, Wang N, Zhang R, Li J, Zhang T, et al. Cerebral ischemia elicits aberration in myocardium contractile function and intracellular calcium handling. Cell Physiol Biochem. 2010;26:421-30 pubmed publisher
  • western blot; rat; 1:1000
Kalofoutis C, Mourouzis I, Galanopoulos G, Dimopoulos A, Perimenis P, Spanou D, et al. Thyroid hormone can favorably remodel the diabetic myocardium after acute myocardial infarction. Mol Cell Biochem. 2010;345:161-9 pubmed publisher
  • western blot; rat
Zeiger U, Mitchell C, Khurana T. Superior calcium homeostasis of extraocular muscles. Exp Eye Res. 2010;91:613-22 pubmed publisher
  • western blot; rat
Neviere R, Hassoun S, Decoster B, Bouazza Y, Montaigne D, Marechal X, et al. Caspase-dependent protein phosphatase 2A activation contributes to endotoxin-induced cardiomyocyte contractile dysfunction. Crit Care Med. 2010;38:2031-6 pubmed publisher
  • western blot; domestic rabbit; 1:1000
Liu Y, Huang H, Xia W, Tang Y, Li H, Huang C. NADPH oxidase inhibition ameliorates cardiac dysfunction in rabbits with heart failure. Mol Cell Biochem. 2010;343:143-53 pubmed publisher
  • immunohistochemistry; human; 1:50
Di Blasi C, Blasevich F, Bellafiore E, Mottarelli E, Gibertini S, Zanotti S, et al. Calsequestrin and junctin immunoreactivity in hexagonally cross-linked tubular arrays myopathy. Neuromuscul Disord. 2010;20:326-9 pubmed publisher
  • western blot; rat; 1:5000
Aguiar C, Andrade V, Gomes E, Alves M, Ladeira M, Pinheiro A, et al. Succinate modulates Ca(2+) transient and cardiomyocyte viability through PKA-dependent pathway. Cell Calcium. 2010;47:37-46 pubmed publisher
  • western blot; human; 1:2000
Donoghue P, Hughes C, Vissers J, Langridge J, Dunn M. Nonionic detergent phase extraction for the proteomic analysis of heart membrane proteins using label-free LC-MS. Proteomics. 2008;8:3895-905 pubmed publisher
  • western blot; mouse
Rizzi N, Liu N, Napolitano C, Nori A, Turcato F, Colombi B, et al. Unexpected structural and functional consequences of the R33Q homozygous mutation in cardiac calsequestrin: a complex arrhythmogenic cascade in a knock in mouse model. Circ Res. 2008;103:298-306 pubmed publisher
  • western blot; mouse
Zhu M, Gach A, Liu G, Xu X, Lim C, Zhang J, et al. Enhanced calcium cycling and contractile function in transgenic hearts expressing constitutively active G alpha o* protein. Am J Physiol Heart Circ Physiol. 2008;294:H1335-47 pubmed publisher
  • western blot; rat; 1:3,000
Birkeland J, Swift F, Tovsrud N, Enger U, Lunde P, Qvigstad E, et al. Serotonin increases L-type Ca2+ current and SR Ca2+ content through 5-HT4 receptors in failing rat ventricular cardiomyocytes. Am J Physiol Heart Circ Physiol. 2007;293:H2367-76 pubmed
  • western blot; mouse
Szczesna Cordary D, Jones M, Moore J, Watt J, Kerrick W, Xu Y, et al. Myosin regulatory light chain E22K mutation results in decreased cardiac intracellular calcium and force transients. FASEB J. 2007;21:3974-85 pubmed
  • western blot; rat; 1:1,000
Pantos C, Mourouzis I, Markakis K, Dimopoulos A, Xinaris C, Kokkinos A, et al. Thyroid hormone attenuates cardiac remodeling and improves hemodynamics early after acute myocardial infarction in rats. Eur J Cardiothorac Surg. 2007;32:333-9 pubmed
  • immunocytochemistry; mouse
  • western blot; mouse; 1:2000
Stenoien D, Knyushko T, Londono M, Opresko L, Mayer M, Brady S, et al. Cellular trafficking of phospholamban and formation of functional sarcoplasmic reticulum during myocyte differentiation. Am J Physiol Cell Physiol. 2007;292:C2084-94 pubmed
  • western blot; human
Armoundas A, Rose J, Aggarwal R, Stuyvers B, O ROURKE B, Kass D, et al. Cellular and molecular determinants of altered Ca2+ handling in the failing rabbit heart: primary defects in SR Ca2+ uptake and release mechanisms. Am J Physiol Heart Circ Physiol. 2007;292:H1607-18 pubmed
  • western blot; rat; 1:1,000
Rodriguez P, Mitton B, Waggoner J, Kranias E. Identification of a novel phosphorylation site in protein phosphatase inhibitor-1 as a negative regulator of cardiac function. J Biol Chem. 2006;281:38599-608 pubmed
  • western blot; mouse
Takahashi T, Tang T, Lai N, Roth D, Rebolledo B, Saito M, et al. Increased cardiac adenylyl cyclase expression is associated with increased survival after myocardial infarction. Circulation. 2006;114:388-96 pubmed
  • western blot; pigs ; 1:500
  • western blot; human; 1:500
Dolnikov K, Shilkrut M, Zeevi Levin N, Gerecht Nir S, Amit M, Danon A, et al. Functional properties of human embryonic stem cell-derived cardiomyocytes: intracellular Ca2+ handling and the role of sarcoplasmic reticulum in the contraction. Stem Cells. 2006;24:236-45 pubmed
  • western blot; rat; 1:2,000
Yamamura K, Steenbergen C, Murphy E. Protein kinase C and preconditioning: role of the sarcoplasmic reticulum. Am J Physiol Heart Circ Physiol. 2005;289:H2484-90 pubmed
  • western blot; mouse
Rothermel B, Berenji K, Tannous P, Kutschke W, Dey A, Nolan B, et al. Differential activation of stress-response signaling in load-induced cardiac hypertrophy and failure. Physiol Genomics. 2005;23:18-27 pubmed
  • western blot; mouse
Tavi P, Sjogren M, Lunde P, Zhang S, Abbate F, Vennstrom B, et al. Impaired Ca2+ handling and contraction in cardiomyocytes from mice with a dominant negative thyroid hormone receptor alpha1. J Mol Cell Cardiol. 2005;38:655-63 pubmed
  • western blot; mouse
Nakayama M, Yan X, Price R, Borg T, Ito K, Sanbe A, et al. Chronic ventricular myocyte-specific overexpression of angiotensin II type 2 receptor results in intrinsic myocyte contractile dysfunction. Am J Physiol Heart Circ Physiol. 2005;288:H317-27 pubmed
  • western blot; rat; 1:1,000
Coutu P, Metzger J. Genetic manipulation of calcium-handling proteins in cardiac myocytes. I. Experimental studies. Am J Physiol Heart Circ Physiol. 2005;288:H601-12 pubmed
Liu T, Xiong F, Qi X, Xiao J, Villeneuve L, Abu Taha I, et al. Altered calcium handling produces reentry-promoting action potential alternans in atrial fibrillation-remodeled hearts. JCI Insight. 2020;5: pubmed publisher
Hadipour Lakmehsari S, Driouchi A, Lee S, Kuzmanov U, Callaghan N, Heximer S, et al. Nanoscale reorganization of sarcoplasmic reticulum in pressure-overload cardiac hypertrophy visualized by dSTORM. Sci Rep. 2019;9:7867 pubmed publisher
Miranda Silva D, Gonçalves Rodrigues P, Almeida Coelho J, Hamdani N, Lima T, Conceição G, et al. Characterization of biventricular alterations in myocardial (reverse) remodelling in aortic banding-induced chronic pressure overload. Sci Rep. 2019;9:2956 pubmed publisher
Liu R, Miller C, D Annibale C, Vo K, Jacobs A. Differential localizations of protein phosphatase 1 isoforms determine their physiological function in the heart. Acta Biochim Biophys Sin (Shanghai). 2019;51:323-330 pubmed publisher
Koyani C, Trummer C, Shrestha N, Scheruebel S, Bourgeois B, Plastira I, et al. Saxagliptin but Not Sitagliptin Inhibits CaMKII and PKC via DPP9 Inhibition in Cardiomyocytes. Front Physiol. 2018;9:1622 pubmed publisher
Makarewich C, Munir A, Schiattarella G, Bezprozvannaya S, Raguimova O, Cho E, et al. The DWORF micropeptide enhances contractility and prevents heart failure in a mouse model of dilated cardiomyopathy. elife. 2018;7: pubmed publisher
Ng K, Lau Y, Dhandhania V, Cai Z, Lee Y, Lai W, et al. Empagliflozin Ammeliorates High Glucose Induced-Cardiac Dysfuntion in Human iPSC-Derived Cardiomyocytes. Sci Rep. 2018;8:14872 pubmed publisher
Kushnir A, Santulli G, Reiken S, Coromilas E, Godfrey S, Brunjes D, et al. Ryanodine Receptor Calcium Leak in Circulating B-Lymphocytes as a Biomarker in Heart Failure. Circulation. 2018;: pubmed publisher
Lee S, Hadipour Lakmehsari S, Miyake T, Gramolini A. Three-dimensional imaging reveals endo(sarco)plasmic reticulum-containing invaginations within the nucleoplasm of muscle. Am J Physiol Cell Physiol. 2018;314:C257-C267 pubmed publisher
Liu G, Zhu H, Cai W, Wang X, Jiang M, Essandoh K, et al. Regulation of BECN1-Mediated Autophagy by HSPB6: Insights from A Human HSPB6S10F Mutant. Autophagy. 2017;:1-56 pubmed publisher
Jacobsen B, Leopoldo A, Cordeiro J, Campos D, Nascimento A, Sugizaki M, et al. Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity. Arq Bras Cardiol. 2017;: pubmed publisher
Sacchi V, Wang B, Kubli D, Martinez A, Jin J, Alvarez R, et al. Peptidyl-Prolyl Isomerase 1 Regulates Ca2+ Handling by Modulating Sarco(Endo)Plasmic Reticulum Calcium ATPase and Na2+/Ca2+ Exchanger 1 Protein Levels and Function. J Am Heart Assoc. 2017;6: pubmed publisher
Patel B, Raad M, Sebag I, Chalifour L. Sex-specific cardiovascular responses to control or high fat diet feeding in C57bl/6 mice chronically exposed to bisphenol A. Toxicol Rep. 2015;2:1310-1318 pubmed publisher
Li Y, Baldwin T, Wang Y, Subramaniam J, Carbajal A, Brand C, et al. Loss of type 9 adenylyl cyclase triggers reduced phosphorylation of Hsp20 and diastolic dysfunction. Sci Rep. 2017;7:5522 pubmed publisher
Røe A, Aronsen J, Skårdal K, Hamdani N, Linke W, Danielsen H, et al. Increased passive stiffness promotes diastolic dysfunction despite improved Ca2+ handling during left ventricular concentric hypertrophy. Cardiovasc Res. 2017;113:1161-1172 pubmed publisher
Ravel Chapuis A, Belanger G, Cote J, Michel R, Jasmin B. Misregulation of calcium-handling proteins promotes hyperactivation of calcineurin-NFAT signaling in skeletal muscle of DM1 mice. Hum Mol Genet. 2017;26:2192-2206 pubmed publisher
Barbagallo F, Xu B, Reddy G, West T, Wang Q, Fu Q, et al. Genetically Encoded Biosensors Reveal PKA Hyperphosphorylation on the Myofilaments in Rabbit Heart Failure. Circ Res. 2016;119:931-43 pubmed publisher
Manning J, Withers C, Levitan B, Smith J, Andres D, Satin J. Loss of Rad-GTPase produces a novel adaptive cardiac phenotype resistant to systolic decline with aging. Am J Physiol Heart Circ Physiol. 2015;309:H1336-45 pubmed publisher
Lugenbiel P, Wenz F, Govorov K, Schweizer P, Katus H, Thomas D. Atrial fibrillation complicated by heart failure induces distinct remodeling of calcium cycling proteins. PLoS ONE. 2015;10:e0116395 pubmed publisher
Traister A, Li M, Aafaqi S, Lu M, Arab S, Radisic M, et al. Integrin-linked kinase mediates force transduction in cardiomyocytes by modulating SERCA2a/PLN function. Nat Commun. 2014;5:4533 pubmed publisher
Li N, Chiang D, Wang S, Wang Q, Sun L, Voigt N, et al. Ryanodine receptor-mediated calcium leak drives progressive development of an atrial fibrillation substrate in a transgenic mouse model. Circulation. 2014;129:1276-1285 pubmed publisher
Cezar M, Damatto R, Martinez P, Lima A, Campos D, Rosa C, et al. Aldosterone blockade reduces mortality without changing cardiac remodeling in spontaneously hypertensive rats. Cell Physiol Biochem. 2013;32:1275-87 pubmed publisher
Gurha P, Abreu Goodger C, Wang T, Ramirez M, Drumond A, van Dongen S, et al. Targeted deletion of microRNA-22 promotes stress-induced cardiac dilation and contractile dysfunction. Circulation. 2012;125:2751-61 pubmed publisher
Desroches B, Zhang P, Choi B, King M, Maldonado A, Li W, et al. Functional scaffold-free 3-D cardiac microtissues: a novel model for the investigation of heart cells. Am J Physiol Heart Circ Physiol. 2012;302:H2031-42 pubmed publisher
Xie Y, Chen B, Sanders P, Guo A, Li Y, Zimmerman K, et al. Sildenafil prevents and reverses transverse-tubule remodeling and Ca(2+) handling dysfunction in right ventricle failure induced by pulmonary artery hypertension. Hypertension. 2012;59:355-62 pubmed publisher
Stangherlin A, Gesellchen F, Zoccarato A, Terrin A, Fields L, Berrera M, et al. cGMP signals modulate cAMP levels in a compartment-specific manner to regulate catecholamine-dependent signaling in cardiac myocytes. Circ Res. 2011;108:929-39 pubmed publisher
Kushnir A, Shan J, Betzenhauser M, Reiken S, Marks A. Role of CaMKIIdelta phosphorylation of the cardiac ryanodine receptor in the force frequency relationship and heart failure. Proc Natl Acad Sci U S A. 2010;107:10274-9 pubmed publisher
Wang J, Chan T, Zhang X, Gao E, Song J, Koch W, et al. Induced overexpression of Na+/Ca2+ exchanger transgene: altered myocyte contractility, [Ca2+]i transients, SR Ca2+ contents, and action potential duration. Am J Physiol Heart Circ Physiol. 2009;297:H590-601 pubmed publisher
Prasad A, Inesi G. Effects of thapsigargin and phenylephrine on calcineurin and protein kinase C signaling functions in cardiac myocytes. Am J Physiol Cell Physiol. 2009;296:C992-C1002 pubmed publisher
Aiba T, Hesketh G, Barth A, Liu T, Daya S, Chakir K, et al. Electrophysiological consequences of dyssynchronous heart failure and its restoration by resynchronization therapy. Circulation. 2009;119:1220-30 pubmed publisher
Shao C, Wehrens X, Wyatt T, Parbhu S, Rozanski G, Patel K, et al. Exercise training during diabetes attenuates cardiac ryanodine receptor dysregulation. J Appl Physiol (1985). 2009;106:1280-92 pubmed publisher
Fernandez Velasco M, Rueda A, Rizzi N, Benitah J, Colombi B, Napolitano C, et al. Increased Ca2+ sensitivity of the ryanodine receptor mutant RyR2R4496C underlies catecholaminergic polymorphic ventricular tachycardia. Circ Res. 2009;104:201-9, 12p following 209 pubmed publisher
Prasad V, Bodi I, Meyer J, Wang Y, Ashraf M, Engle S, et al. Impaired cardiac contractility in mice lacking both the AE3 Cl-/HCO3- exchanger and the NKCC1 Na+-K+-2Cl- cotransporter: effects on Ca2+ handling and protein phosphatases. J Biol Chem. 2008;283:31303-14 pubmed publisher
Ukropec J, Anunciado R, Ravussin Y, Hulver M, Kozak L. UCP1-independent thermogenesis in white adipose tissue of cold-acclimated Ucp1-/- mice. J Biol Chem. 2006;281:31894-908 pubmed
Takaseya T, Ishimatsu M, Tayama E, Nishi A, Akasu T, Aoyagi S. Mechanical unloading improves intracellular Ca2+ regulation in rats with doxorubicin-induced cardiomyopathy. J Am Coll Cardiol. 2004;44:2239-46 pubmed
van der Velden J, Merkus D, Klarenbeek B, James A, Boontje N, Dekkers D, et al. Alterations in myofilament function contribute to left ventricular dysfunction in pigs early after myocardial infarction. Circ Res. 2004;95:e85-95 pubmed
Huke S, Periasamy M. Phosphorylation-status of phospholamban and calsequestrin modifies their affinity towards commonly used antibodies. J Mol Cell Cardiol. 2004;37:795-9 pubmed
Liu J, Hu Q, Wang Z, Xu C, Wang X, Gong G, et al. Autologous stem cell transplantation for myocardial repair. Am J Physiol Heart Circ Physiol. 2004;287:H501-11 pubmed
Suarez J, Gloss B, Belke D, Hu Y, Scott B, Dieterle T, et al. Doxycycline inducible expression of SERCA2a improves calcium handling and reverts cardiac dysfunction in pressure overload-induced cardiac hypertrophy. Am J Physiol Heart Circ Physiol. 2004;287:H2164-72 pubmed
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product information
Product Type :
Antibody
Product Name :
Phospholamban Monoclonal Antibody (2D12)
Catalog # :
MA3-922
Quantity :
100 µg
Price :
US 399.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Canine, Chicken, Guinea Pig, Human, Mouse, Ovine, Porcine, Rabbit, Rat
Applications :
Immunocytochemistry: 1:10-1:100, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:5,000
Species :
Canine, Chicken, Guinea Pig, Human, Mouse, Ovine, Porcine, Rabbit, Rat
Clone :
2D12
Isotype :
IgG2a
Storage :
-20° C, Avoid Freeze/Thaw Cycles
Description :
Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer.
Immunogen :
Synthetic peptide corresponding to residues D(2) K V Q Y L T R S A I R R A S T I E M P Q Q A R(25) of canine Phospholamban.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1:10-1:100, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:5,000
Aliases :
cardiac CMH18; Cardiac phospholamban; CMD1P; CMH18; phospholamban; Plb; Plm; PLN; unnamed protein product
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA