CACNA2D1 Monoclonal Antibody (20A) | Invitrogen MA3-921 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CACNA2D1 Monoclonal Antibody (20A)

catalog :

MA3-921

quantity :

100 µL

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

20A

reactivity :

Japanese rice fish, guinea pig, human, mouse, rat, pigs , domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 45

Published Application/Species/Sample/Dilution	Reference
western blot; rat; loading ...; fig 3b	Kim T, Terentyeva R, Roder K, Li W, Liu M, Greener I, et al. SK channel enhancers attenuate Ca2+-dependent arrhythmia in hypertrophic hearts by regulating mito-ROS-dependent oxidation and activity of RyR. Cardiovasc Res. 2017;113:343-353 pubmed publisher
western blot; mouse; 1:500; loading ...; fig 1b	Oláh T, Bodnár D, Tóth A, Vincze J, Fodor J, Reischl B, et al. Cannabinoid signalling inhibits sarcoplasmic Ca2+ release and regulates excitation-contraction coupling in mammalian skeletal muscle. J Physiol. 2016;594:7381-7398 pubmed publisher
immunocytochemistry; domestic rabbit; fig 1	Mahalingam M, Perez C, Fessenden J. Fluorescence Resonance Energy Transfer-based Structural Analysis of the Dihydropyridine Receptor ?1S Subunit Reveals Conformational Differences Induced by Binding of the ?1a Subunit. J Biol Chem. 2016;291:13762-70 pubmed publisher
immunocytochemistry; mouse	Eltit J, Franzini Armstrong C, Perez C. Amino acid residues 489-503 of dihydropyridine receptor (DHPR) Î²1a subunit are critical for structural communication between the skeletal muscle DHPR complex and type 1 ryanodine receptor. J Biol Chem. 2014;289:36116-24 pubmed publisher
western blot; mouse	Groenke S, Larson E, Alber S, Zhang R, Lamp S, Ren X, et al. Complete atrial-specific knockout of sodium-calcium exchange eliminates sinoatrial node pacemaker activity. PLoS ONE. 2013;8:e81633 pubmed publisher
western blot; mouse	Nader M, Westendorp B, Hawari O, Salih M, Stewart A, Leenen F, et al. Tail-anchored membrane protein SLMAP is a novel regulator of cardiac function at the sarcoplasmic reticulum. Am J Physiol Heart Circ Physiol. 2012;302:H1138-45 pubmed publisher
western blot; domestic rabbit; 1:300	DiFranco M, Tran P, Quiñonez M, Vergara J. Functional expression of transgenic 1sDHPR channels in adult mammalian skeletal muscle fibres. J Physiol. 2011;589:1421-42 pubmed publisher
immunohistochemistry; rat	Dassau L, Conti L, Radeke C, Ptacek L, Vandenberg C. Kir2.6 regulates the surface expression of Kir2.x inward rectifier potassium channels. J Biol Chem. 2011;286:9526-41 pubmed publisher
western blot; mouse	Al Moamen N, Prasad V, Bodi I, Miller M, Neiman M, Lasko V, et al. Loss of the AE3 anion exchanger in a hypertrophic cardiomyopathy model causes rapid decompensation and heart failure. J Mol Cell Cardiol. 2011;50:137-46 pubmed publisher
western blot; mouse; 1:500	Bueno C, Ferreira J, Pereira M, Bacurau A, Brum P. Aerobic exercise training improves skeletal muscle function and Ca2+ handling-related protein expression in sympathetic hyperactivity-induced heart failure. J Appl Physiol (1985). 2010;109:702-9 pubmed publisher
western blot; mouse; 1:500	Ferreira J, Bacurau A, Bueno C, Cunha T, Tanaka L, Jardim M, et al. Aerobic exercise training improves Ca2+ handling and redox status of skeletal muscle in mice. Exp Biol Med (Maywood). 2010;235:497-505 pubmed publisher
flow cytometry; mouse immunocytochemistry; mouse; 1:300	Grajales L, Garcia J, Banach K, Geenen D. Delayed enrichment of mesenchymal cells promotes cardiac lineage and calcium transient development. J Mol Cell Cardiol. 2010;48:735-45 pubmed publisher
western blot; mouse	Louch W, Hougen K, Mørk H, Swift F, Aronsen J, Sjaastad I, et al. Sodium accumulation promotes diastolic dysfunction in end-stage heart failure following Serca2 knockout. J Physiol. 2010;588:465-78 pubmed publisher
immunohistochemistry; rat western blot; rat	O CONNELL K, Ohlendieck K. Proteomic DIGE analysis of the mitochondria-enriched fraction from aged rat skeletal muscle. Proteomics. 2009;9:5509-24 pubmed publisher
western blot; mouse	Mørk H, Sjaastad I, Sejersted O, Louch W. Slowing of cardiomyocyte Ca2+ release and contraction during heart failure progression in postinfarction mice. Am J Physiol Heart Circ Physiol. 2009;296:H1069-79 pubmed publisher
western blot; mouse; 1:500	Ackermann G, Domenighetti A, Deten A, Bonath I, Marenholz I, Pedrazzini T, et al. S100A1 deficiency results in prolonged ventricular repolarization in response to sympathetic activation. Gen Physiol Biophys. 2008;27:127-42 pubmed
immunocytochemistry; human	Tishkoff D, Nibbelink K, Holmberg K, Dandu L, Simpson R. Functional vitamin D receptor (VDR) in the t-tubules of cardiac myocytes: VDR knockout cardiomyocyte contractility. Endocrinology. 2008;149:558-64 pubmed
immunohistochemistry; Japanese rice fish; 1:200	Hirata H, Watanabe T, Hatakeyama J, Sprague S, Saint Amant L, Nagashima A, et al. Zebrafish relatively relaxed mutants have a ryanodine receptor defect, show slow swimming and provide a model of multi-minicore disease. Development. 2007;134:2771-81 pubmed
western blot; mouse	Mørk H, Sjaastad I, Sande J, Periasamy M, Sejersted O, Louch W. Increased cardiomyocyte function and Ca2+ transients in mice during early congestive heart failure. J Mol Cell Cardiol. 2007;43:177-86 pubmed
western blot; pigs	Schulz J, Palmer N, Steckelberg J, Jones S, Zeece M. Microarray profiling of skeletal muscle sarcoplasmic reticulum proteins. Biochim Biophys Acta. 2006;1764:1429-35 pubmed
western blot; mouse	Doran P, Dowling P, Donoghue P, Buffini M, Ohlendieck K. Reduced expression of regucalcin in young and aged mdx diaphragm indicates abnormal cytosolic calcium handling in dystrophin-deficient muscle. Biochim Biophys Acta. 2006;1764:773-85 pubmed
western blot; rat; 1:5,000	Collins H, Loka A, DiCarlo S. Daily exercise-induced cardioprotection is associated with changes in calcium regulatory proteins in hypertensive rats. Am J Physiol Heart Circ Physiol. 2005;288:H532-40 pubmed
	Coste de Bagneaux P, von Elsner L, Bierhals T, Campiglio M, Johannsen J, Obermair G, et al. A homozygous missense variant in CACNB4 encoding the auxiliary calcium channel beta4 subunit causes a severe neurodevelopmental disorder and impairs channel and non-channel functions. PLoS Genet. 2020;16:e1008625 pubmed publisher
	Huang C, Wang Z, Zhang K, Dong Y, Zhang A, Lu C, et al. MicroRNA-107 inhibits proliferation and invasion of laryngeal squamous cell carcinoma cells by targeting CACNA2D1 in vitro. Anticancer Drugs. 2020;31:260-271 pubmed publisher
	Huang C, Li Y, Zhao W, Zhang A, Lu C, Wang Z, et al. α2δ1 may be a potential marker for cancer stem cell in laryngeal squamous cell carcinoma. Cancer Biomark. 2019;24:97-107 pubmed publisher
	Sharma P, Abbasi C, Lazic S, Teng A, Wang D, Dubois N, et al. Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function. Nat Commun. 2015;6:8391 pubmed publisher
	Eltit J, Szpyt J, Li H, Allen P, Perez C. Reduced gain of excitation-contraction coupling in triadin-null myotubes is mediated by the disruption of FKBP12/RyR1 interaction. Cell Calcium. 2011;49:128-35 pubmed publisher
	Rasmussen H, Møller M, Knaus H, Jensen B, Olesen S, Jørgensen N. Subcellular localization of the delayed rectifier K(+) channels KCNQ1 and ERG1 in the rat heart. Am J Physiol Heart Circ Physiol. 2004;286:H1300-9 pubmed
	Glover L, Heffron J, Ohlendieck K. Increased sensitivity of the ryanodine receptor to halothane-induced oligomerization in malignant hyperthermia-susceptible human skeletal muscle. J Appl Physiol (1985). 2004;96:11-8 pubmed
	Wolf M, Eberhart A, Glossmann H, Striessnig J, Grigorieff N. Visualization of the domain structure of an L-type Ca2+ channel using electron cryo-microscopy. J Mol Biol. 2003;332:171-82 pubmed
	Mulvey C, Ohlendieck K. Use of continuous-elution gel electrophoresis as a preparative tool for blot overlay analysis. Anal Biochem. 2003;319:122-30 pubmed
	Dowling P, Lohan J, Ohlendieck K. Comparative analysis of Dp427-deficient mdx tissues shows that the milder dystrophic phenotype of extraocular and toe muscle fibres is associated with a persistent expression of beta-dystroglycan. Eur J Cell Biol. 2003;82:222-30 pubmed
	Most P, Remppis A, Pleger S, Löffler E, Ehlermann P, Bernotat J, et al. Transgenic overexpression of the Ca2+-binding protein S100A1 in the heart leads to increased in vivo myocardial contractile performance. J Biol Chem. 2003;278:33809-17 pubmed
	Moran M, Saborido A, Megías A. Ca2+ regulatory systems in rat myocardium are altered by 24 weeks treadmill training. Pflugers Arch. 2003;446:161-8 pubmed
	Culligan K, Banville N, Dowling P, Ohlendieck K. Drastic reduction of calsequestrin-like proteins and impaired calcium binding in dystrophic mdx muscle. J Appl Physiol (1985). 2002;92:435-45 pubmed
	Glover L, Culligan K, Cala S, Mulvey C, Ohlendieck K. Calsequestrin binds to monomeric and complexed forms of key calcium-handling proteins in native sarcoplasmic reticulum membranes from rabbit skeletal muscle. Biochim Biophys Acta. 2001;1515:120-32 pubmed
	Marais E, Klugbauer N, Hofmann F. Calcium channel alpha(2)delta subunits-structure and Gabapentin binding. Mol Pharmacol. 2001;59:1243-8 pubmed
	Froemming G, Ohlendieck K. Native skeletal muscle dihydropyridine receptor exists as a supramolecular triad complex. Cell Mol Life Sci. 2001;58:312-20 pubmed
	Harmon S, Froemming G, Leisner E, Pette D, Ohlendieck K. Low-frequency stimulation of fast muscle affects the abundance of Ca(2+)-ATPase but not its oligomeric status. J Appl Physiol (1985). 2001;90:371-9 pubmed
	Froemming G, Murray B, Harmon S, Pette D, Ohlendieck K. Comparative analysis of the isoform expression pattern of Ca(2+)-regulatory membrane proteins in fast-twitch, slow-twitch, cardiac, neonatal and chronic low-frequency stimulated muscle fibers. Biochim Biophys Acta. 2000;1466:151-68 pubmed
	Ohlendieck K, Frömming G, Murray B, Maguire P, Leisner E, Traub I, et al. Effects of chronic low-frequency stimulation on Ca2+-regulatory membrane proteins in rabbit fast muscle. Pflugers Arch. 1999;438:700-8 pubmed
	Protasi F, Franzini Armstrong C, Allen P. Role of ryanodine receptors in the assembly of calcium release units in skeletal muscle. J Cell Biol. 1998;140:831-42 pubmed
	Morton M, Cassidy T, Froehner S, Gilmour B, Laurens R. Alpha 1 and alpha 2 Ca2+ channel subunit expression in human neuronal and small cell carcinoma cells. FASEB J. 1994;8:884-8 pubmed
	Morton M, Froehner S. The alpha 1 and alpha 2 polypeptides of the dihydropyridine-sensitive calcium channel differ in developmental expression and tissue distribution. Neuron. 1989;2:1499-506 pubmed
	Flucher B, Morton M, Froehner S, Daniels M. Localization of the alpha 1 and alpha 2 subunits of the dihydropyridine receptor and ankyrin in skeletal muscle triads. Neuron. 1990;5:339-51 pubmed

product information

Product Type :

Antibody

Product Name :

CACNA2D1 Monoclonal Antibody (20A)

Catalog # :

MA3-921

Quantity :

100 µL

Price :

US 399.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Guinea Pig, Human, Mouse, Rabbit, Rat

Applications :

Flow Cytometry: 1:100-1:200, Immunocytochemistry: 1:250, Immunohistochemistry (Frozen): 1:500, Immunohistochemistry (Paraffin): 1:500, Western Blot: 1:500-1:1,000

Species :

Guinea Pig, Human, Mouse, Rabbit, Rat

Clone :

20A

Isotype :

IgG2a

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

This gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Immunogen :

Purified rabbit dihydropyridine receptor.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1:100-1:200, Immunocytochemistry: 1:250, Immunohistochemistry (Frozen): 1:500, Immunohistochemistry (Paraffin): 1:500, Western Blot: 1:500-1:1,000

Aliases :

Ca(v)alpha2delta1; Cacna2; Cacna2d1; CACNL2A; Calcium channel subunit alpha 2 delta (dihydropyridine - sensitive L-type); calcium channel, L type, alpha 2 polypeptide; calcium channel, voltage-dependent, alpha 2/delta subunit 1; calcium channel, voltage-dependent, alpha2/delta subunit 1; calcium voltage-gated channel auxiliary subunit alpha2delta 1; CCHL2A; CCHLA2; DHP; DHSCCA; dihydropryridine-sensitive calcium channel alpha-2 subunit; dihydropyridine-sensitive calcium channel alpha 2; dihydropyridine-sensitive L-type, calcium channel alpha-2/delta subunit; H_DJ0560O14.1; LINC01112; lncRNA-N3; MHS3; unknown protein; voltage-dependent calcium channel alpha-2 delta subunit precursor; Voltage-dependent calcium channel subunit alpha-2/delta-1; Voltage-dependent calcium channel subunit alpha-2-1; Voltage-dependent calcium channel subunit delta-1; Voltage-gated calcium channel subunit alpha-2/delta-1; WUGSC:H_DJ0560O14.1

more info or order :

Invitrogen product webpage