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company name :
Invitrogen
other brands :
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product type :
antibody
product name :
CaV1.1 Monoclonal Antibody (1A)
catalog :
MA3-920
quantity :
200 µL
price :
US 399.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1A
reactivity :
Japanese rice fish, guinea pig, human, mouse, rat, zebrafish , domestic rabbit
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 71
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:1000; loading ...; fig s2f
Lee C, Hanna A, Wang H, Dagnino Acosta A, Joshi A, Knoblauch M, et al. A chemical chaperone improves muscle function in mice with a RyR1 mutation. Nat Commun. 2017;8:14659 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 1b
Amici D, Pinal Fernández I, Mázala D, Lloyd T, Corse A, Christopher Stine L, et al. Calcium dysregulation, functional calpainopathy, and endoplasmic reticulum stress in sporadic inclusion body myositis. Acta Neuropathol Commun. 2017;5:24 pubmed publisher
  • western blot; mouse; loading ...; fig 2e
Fajardo V, Gamu D, Mitchell A, Bloemberg D, Bombardier E, Chambers P, et al. Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice. PLoS ONE. 2017;12:e0173708 pubmed publisher
  • immunohistochemistry; zebrafish ; 1:100; loading ...; fig 1a
Linsley J, Hsu I, Groom L, Yarotskyy V, Lavorato M, Horstick E, et al. Congenital myopathy results from misregulation of a muscle Ca2+ channel by mutant Stac3. Proc Natl Acad Sci U S A. 2017;114:E228-E236 pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 5a
Kanzaki K, Watanabe D, Kuratani M, Yamada T, Matsunaga S, Wada M. Role of calpain in eccentric contraction-induced proteolysis of Ca2+-regulatory proteins and force depression in rat fast-twitch skeletal muscle. J Appl Physiol (1985). 2017;122:396-405 pubmed publisher
  • western blot; mouse; 1:1000; fig 6
Vanhoutte D, Schips T, Kwong J, Davis J, Tjondrokoesoemo A, Brody M, et al. Thrombospondin expression in myofibers stabilizes muscle membranes. elife. 2016;5: pubmed publisher
  • western blot; mouse; fig 13
Cong X, Doering J, Mázala D, Chin E, Grange R, Jiang H. The SH3 and cysteine-rich domain 3 (Stac3) gene is important to growth, fiber composition, and calcium release from the sarcoplasmic reticulum in postnatal skeletal muscle. Skelet Muscle. 2016;6:17 pubmed publisher
  • western blot; mouse; 1:1000; fig 5, 6
Tjondrokoesoemo A, Schips T, Kanisicak O, Sargent M, Molkentin J. Genetic overexpression of Serpina3n attenuates muscular dystrophy in mice. Hum Mol Genet. 2016;25:1192-202 pubmed publisher
  • western blot; mouse
Osbourne A, Calway T, Broman M, McSharry S, Earley J, Kim G. Downregulation of connexin43 by microRNA-130a in cardiomyocytes results in cardiac arrhythmias. J Mol Cell Cardiol. 2014;74:53-63 pubmed publisher
  • immunohistochemistry; mouse
Childers M, Joubert R, Poulard K, Moal C, Grange R, Doering J, et al. Gene therapy prolongs survival and restores function in murine and canine models of myotubular myopathy. Sci Transl Med. 2014;6:220ra10 pubmed publisher
  • immunohistochemistry; mouse; 1:100
Posey A, Swanson K, Alvarez M, Krishnan S, Earley J, Band H, et al. EHD1 mediates vesicle trafficking required for normal muscle growth and transverse tubule development. Dev Biol. 2014;387:179-90 pubmed publisher
  • immunocytochemistry; mouse
Kerr J, Ziman A, Mueller A, Muriel J, Kleinhans Welte E, Gumerson J, et al. Dysferlin stabilizes stress-induced Ca2+ signaling in the transverse tubule membrane. Proc Natl Acad Sci U S A. 2013;110:20831-6 pubmed publisher
  • western blot; mouse; 1:3000
Demonbreun A, Rossi A, Alvarez M, Swanson K, Deveaux H, Earley J, et al. Dysferlin and myoferlin regulate transverse tubule formation and glycerol sensitivity. Am J Pathol. 2014;184:248-59 pubmed publisher
  • immunohistochemistry; mouse
Caruso N, Herberth B, Bartoli M, Puppo F, Dumonceaux J, Zimmermann A, et al. Deregulation of the protocadherin gene FAT1 alters muscle shapes: implications for the pathogenesis of facioscapulohumeral dystrophy. PLoS Genet. 2013;9:e1003550 pubmed publisher
  • immunohistochemistry; mouse
Al Qusairi L, Prokic I, Amoasii L, Kretz C, Messaddeq N, Mandel J, et al. Lack of myotubularin (MTM1) leads to muscle hypotrophy through unbalanced regulation of the autophagy and ubiquitin-proteasome pathways. FASEB J. 2013;27:3384-94 pubmed publisher
  • western blot; mouse
Amoasii L, Hnia K, Chicanne G, Brech A, Cowling B, Müller M, et al. Myotubularin and PtdIns3P remodel the sarcoplasmic reticulum in muscle in vivo. J Cell Sci. 2013;126:1806-19 pubmed publisher
  • western blot; domestic rabbit
Chou C, Chang P, Wen M, Lee H, Chu Y, Baba A, et al. Effects of SEA0400 on arrhythmogenicity in a Langendorff-perfused 1-month myocardial infarction rabbit model. Pacing Clin Electrophysiol. 2013;36:596-606 pubmed publisher
  • western blot; mouse
Szpyt J, Lorenzon N, Perez C, Norris E, Allen P, Beam K, et al. Three-dimensional localization of the α and β subunits and of the II-III loop in the skeletal muscle L-type Ca2+ channel. J Biol Chem. 2012;287:43853-61 pubmed publisher
  • western blot; human
Middlekauff H, Vigna C, Verity M, Fonarow G, Horwich T, Hamilton M, et al. Abnormalities of calcium handling proteins in skeletal muscle mirror those of the heart in humans with heart failure: a shared mechanism?. J Card Fail. 2012;18:724-33 pubmed publisher
  • western blot; mouse; 1:400
Tang Z, Yarotskyy V, Wei L, Sobczak K, Nakamori M, Eichinger K, et al. Muscle weakness in myotonic dystrophy associated with misregulated splicing and altered gating of Ca(V)1.1 calcium channel. Hum Mol Genet. 2012;21:1312-24 pubmed publisher
  • western blot; mouse
Garcia J. The calcium channel ?2/?1 subunit interacts with ATP5b in the plasma membrane of developing muscle cells. Am J Physiol Cell Physiol. 2011;301:C44-52 pubmed publisher
  • immunohistochemistry; mouse; 1:400
  • western blot; mouse
Eltit J, Li H, Ward C, MOLINSKI T, Pessah I, Allen P, et al. Orthograde dihydropyridine receptor signal regulates ryanodine receptor passive leak. Proc Natl Acad Sci U S A. 2011;108:7046-51 pubmed publisher
  • western blot; mouse
Ryan T, Sharma P, Ignatchenko A, MacLennan D, Kislinger T, Gramolini A. Identification of novel ryanodine receptor 1 (RyR1) protein interaction with calcium homeostasis endoplasmic reticulum protein (CHERP). J Biol Chem. 2011;286:17060-8 pubmed publisher
  • immunohistochemistry; mouse
Toussaint A, Cowling B, Hnia K, Mohr M, Oldfors A, Schwab Y, et al. Defects in amphiphysin 2 (BIN1) and triads in several forms of centronuclear myopathies. Acta Neuropathol. 2011;121:253-66 pubmed publisher
  • immunohistochemistry; rat
  • western blot; rat
Kaakinen M, Zelenin S, Metsikkö K. Aquaporin-4 water channel oligomers are associated with the transverse tubules of skeletal myofibers. Exp Cell Res. 2011;317:20-8 pubmed publisher
  • western blot; mouse; 1:500
Bueno C, Ferreira J, Pereira M, Bacurau A, Brum P. Aerobic exercise training improves skeletal muscle function and Ca2+ handling-related protein expression in sympathetic hyperactivity-induced heart failure. J Appl Physiol (1985). 2010;109:702-9 pubmed publisher
  • western blot; mouse; 1:500
Ferreira J, Bacurau A, Bueno C, Cunha T, Tanaka L, Jardim M, et al. Aerobic exercise training improves Ca2+ handling and redox status of skeletal muscle in mice. Exp Biol Med (Maywood). 2010;235:497-505 pubmed publisher
  • western blot; rat
Stefanyk L, Coverdale N, Roy B, Peters S, Leblanc P. Skeletal muscle type comparison of subsarcolemmal mitochondrial membrane phospholipid fatty acid composition in rat. J Membr Biol. 2010;234:207-15 pubmed publisher
  • immunohistochemistry; human; 1:10
Di Blasi C, Blasevich F, Bellafiore E, Mottarelli E, Gibertini S, Zanotti S, et al. Calsequestrin and junctin immunoreactivity in hexagonally cross-linked tubular arrays myopathy. Neuromuscul Disord. 2010;20:326-9 pubmed publisher
  • western blot; rat; 1:1000
Thomas M, Vigna C, Betik A, Tupling A, Hepple R. Initiating treadmill training in late middle age offers modest adaptations in Ca2+ handling but enhances oxidative damage in senescent rat skeletal muscle. Am J Physiol Regul Integr Comp Physiol. 2010;298:R1269-78 pubmed publisher
  • western blot; rat
O CONNELL K, Gannon J, Doran P, Ohlendieck K. Reduced expression of sarcalumenin and related Ca2+ -regulatory proteins in aged rat skeletal muscle. Exp Gerontol. 2008;43:958-61 pubmed publisher
  • western blot; rat
Rasmussen M, Kristensen M, Juel C. Exercise-induced regulation of phospholemman (FXYD1) in rat skeletal muscle: implications for Na+/K+-ATPase activity. Acta Physiol (Oxf). 2008;194:67-79 pubmed publisher
  • immunohistochemistry; Japanese rice fish; 1:200
Hirata H, Watanabe T, Hatakeyama J, Sprague S, Saint Amant L, Nagashima A, et al. Zebrafish relatively relaxed mutants have a ryanodine receptor defect, show slow swimming and provide a model of multi-minicore disease. Development. 2007;134:2771-81 pubmed
  • western blot; human
Xiong L, Zhang J, He R, Hamilton S. A Ca2+-binding domain in RyR1 that interacts with the calmodulin binding site and modulates channel activity. Biophys J. 2006;90:173-82 pubmed
  • immunohistochemistry; rat
Nissinen M, Kaisto T, Salmela P, Peltonen J, Metsikkö K. Restricted distribution of mRNAs encoding a sarcoplasmic reticulum or transverse tubule protein in skeletal myofibers. J Histochem Cytochem. 2005;53:217-27 pubmed
  • immunohistochemistry; mouse; 1:100
Ueda H, Oikawa A, Nakamura A, Terasawa F, Kawagishi K, Moriizumi T. Neuregulin receptor ErbB2 localization at T-tubule in cardiac and skeletal muscle. J Histochem Cytochem. 2005;53:87-91 pubmed
Dayal A, Ng S, Grabner M. Ca2+-activated Cl- channel TMEM16A/ANO1 identified in zebrafish skeletal muscle is crucial for action potential acceleration. Nat Commun. 2019;10:115 pubmed publisher
Berg O, Kwon O, Hureau T, Clifton H, Thurston T, Le Fur Y, et al. Maximal strength training increases muscle force generating capacity and the anaerobic ATP synthesis flux without altering the cost of contraction in elderly. Exp Gerontol. 2018;111:154-161 pubmed publisher
Kim J, Choi S, Lee S, Park K. Voltage-dependent Ca2+ channels promote branching morphogenesis of salivary glands by patterning differential growth. Sci Rep. 2018;8:7566 pubmed publisher
Kanzaki K, Watanabe D, Aibara C, Kawakami Y, Yamada T, Takahashi Y, et al. l-arginine ingestion inhibits eccentric contraction-induced proteolysis and force deficit via S-nitrosylation of calpain. Physiol Rep. 2018;6: pubmed publisher
Cowling B, Prokic I, Tasfaout H, Rabai A, Humbert F, Rinaldi B, et al. Amphiphysin (BIN1) negatively regulates dynamin 2 for normal muscle maturation. J Clin Invest. 2017;127:4477-4487 pubmed publisher
Hofhuis J, Bersch K, Büssenschütt R, Drzymalski M, Liebetanz D, Nikolaev V, et al. Dysferlin mediates membrane tubulation and links T-tubule biogenesis to muscular dystrophy. J Cell Sci. 2017;130:841-852 pubmed publisher
Giudice J, Loehr J, Rodney G, Cooper T. Alternative Splicing of Four Trafficking Genes Regulates Myofiber Structure and Skeletal Muscle Physiology. Cell Rep. 2016;17:1923-1933 pubmed publisher
Demonbreun A, Swanson K, Rossi A, Deveaux H, Earley J, Allen M, et al. Eps 15 Homology Domain (EHD)-1 Remodels Transverse Tubules in Skeletal Muscle. PLoS ONE. 2015;10:e0136679 pubmed publisher
DiFranco M, Yu C, Quiñonez M, Vergara J. Inward rectifier potassium currents in mammalian skeletal muscle fibres. J Physiol. 2015;593:1213-38 pubmed publisher
Chin E, Chen D, Bobyk K, Mázala D. Perturbations in intracellular Ca2+ handling in skeletal muscle in the G93A*SOD1 mouse model of amyotrophic lateral sclerosis. Am J Physiol Cell Physiol. 2014;307:C1031-8 pubmed publisher
Dayal A, Bhat V, Franzini Armstrong C, Grabner M. Domain cooperativity in the ?1a subunit is essential for dihydropyridine receptor voltage sensing in skeletal muscle. Proc Natl Acad Sci U S A. 2013;110:7488-93 pubmed publisher
Liedtke W, Yeo M, Zhang H, Wang Y, Gignac M, Miller S, et al. Highly conductive carbon nanotube matrix accelerates developmental chloride extrusion in central nervous system neurons by increased expression of chloride transporter KCC2. Small. 2013;9:1066-75 pubmed publisher
Cowling B, Toussaint A, Amoasii L, Koebel P, Ferry A, Davignon L, et al. Increased expression of wild-type or a centronuclear myopathy mutant of dynamin 2 in skeletal muscle of adult mice leads to structural defects and muscle weakness. Am J Pathol. 2011;178:2224-35 pubmed publisher
Al Qusairi L, Weiss N, Toussaint A, Berbey C, Messaddeq N, Kretz C, et al. T-tubule disorganization and defective excitation-contraction coupling in muscle fibers lacking myotubularin lipid phosphatase. Proc Natl Acad Sci U S A. 2009;106:18763-8 pubmed publisher
Du W, McMahon T, Zhang Z, Stiber J, Meissner G, Eu J. Excitation-contraction coupling in airway smooth muscle. J Biol Chem. 2006;281:30143-51 pubmed
Radzyukevich T, Heiny J. Regulation of dihydropyridine receptor gene expression in mouse skeletal muscles by stretch and disuse. Am J Physiol Cell Physiol. 2004;287:C1445-52 pubmed
Radzyukevich T, Cougnon M, Moseley A, Heiny J. Developmental induction of DHPR alpha 1s and RYR1 gene expression does not require neural or mechanical signals. J Muscle Res Cell Motil. 2004;25:87-94 pubmed
Glover L, Heffron J, Ohlendieck K. Increased sensitivity of the ryanodine receptor to halothane-induced oligomerization in malignant hyperthermia-susceptible human skeletal muscle. J Appl Physiol (1985). 2004;96:11-8 pubmed
Cougnon M, Moseley A, Radzyukevich T, Lingrel J, Heiny J. Na,K-ATPase alpha- and beta-isoform expression in developing skeletal muscles: alpha(2) correlates with t-tubule formation. Pflugers Arch. 2002;445:123-31 pubmed
Salanova M, Priori G, Barone V, Intravaia E, Flucher B, Ciruela F, et al. Homer proteins and InsP(3) receptors co-localise in the longitudinal sarcoplasmic reticulum of skeletal muscle fibres. Cell Calcium. 2002;32:193-200 pubmed
Papineni R, O Connell K, Zhang H, Dirksen R, Hamilton S. Suramin interacts with the calmodulin binding site on the ryanodine receptor, RYR1. J Biol Chem. 2002;277:49167-74 pubmed
O Neill A, Williams M, Resneck W, Milner D, Capetanaki Y, Bloch R. Sarcolemmal organization in skeletal muscle lacking desmin: evidence for cytokeratins associated with the membrane skeleton at costameres. Mol Biol Cell. 2002;13:2347-59 pubmed
St John P, Gordon H. Agonists cause endocytosis of nicotinic acetylcholine receptors on cultured myotubes. J Neurobiol. 2001;49:212-23 pubmed
Budde T, Sieg F, Braunewell K, Gundelfinger E, Pape H. Ca2+-induced Ca2+ release supports the relay mode of activity in thalamocortical cells. Neuron. 2000;26:483-92 pubmed
Williams M, Bloch R. Extensive but coordinated reorganization of the membrane skeleton in myofibers of dystrophic (mdx) mice. J Cell Biol. 1999;144:1259-70 pubmed
Zoccola D, Tambutt E, S n gas Balas F, Michiels J, Failla J, Jaubert J, et al. Cloning of a calcium channel alpha1 subunit from the reef-building coral, Stylophora pistillata. Gene. 1999;227:157-67 pubmed
Moore R, Nguyen H, Galceran J, Pessah I, Allen P. A transgenic myogenic cell line lacking ryanodine receptor protein for homologous expression studies: reconstitution of Ry1R protein and function. J Cell Biol. 1998;140:843-51 pubmed
Buck E, Nguyen H, Pessah I, Allen P. Dyspedic mouse skeletal muscle expresses major elements of the triadic junction but lacks detectable ryanodine receptor protein and function. J Biol Chem. 1997;272:7360-7 pubmed
Flucher B, Andrews S, Fleischer S, Marks A, Caswell A, Powell J. Triad formation: organization and function of the sarcoplasmic reticulum calcium release channel and triadin in normal and dysgenic muscle in vitro. J Cell Biol. 1993;123:1161-74 pubmed
Morton M, Cassidy T, Froehner S, Gilmour B, Laurens R. Alpha 1 and alpha 2 Ca2+ channel subunit expression in human neuronal and small cell carcinoma cells. FASEB J. 1994;8:884-8 pubmed
Goligorsky M, Colflesh D, Gordienko D, Moore L. Branching points of renal resistance arteries are enriched in L-type calcium channels and initiate vasoconstriction. Am J Physiol. 1995;268:F251-7 pubmed
Morton M, Froehner S. The alpha 1 and alpha 2 polypeptides of the dihydropyridine-sensitive calcium channel differ in developmental expression and tissue distribution. Neuron. 1989;2:1499-506 pubmed
Morton M, Caffrey J, Brown A, Froehner S. Monoclonal antibody to the alpha 1-subunit of the dihydropyridine-binding complex inhibits calcium currents in BC3H1 myocytes. J Biol Chem. 1988;263:613-6 pubmed
Morton M, Froehner S. Monoclonal antibody identifies a 200-kDa subunit of the dihydropyridine-sensitive calcium channel. J Biol Chem. 1987;262:11904-7 pubmed
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product information
Product Type :
Antibody
Product Name :
CaV1.1 Monoclonal Antibody (1A)
Catalog # :
MA3-920
Quantity :
200 µL
Price :
US 399.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Guinea Pig, Human, Mouse, Rabbit, Rat
Applications :
Flow Cytometry: 1 µg/test, Immunohistochemistry (Frozen): 1:200, Immunohistochemistry (Paraffin): 1:20, Immunoprecipitation: Assay-dependent, Western Blot: 1:500
Species :
Guinea Pig, Human, Mouse, Rabbit, Rat
Clone :
1A
Isotype :
IgG1
Storage :
-20° C, Avoid Freeze/Thaw Cycles
Description :
Voltage-sensitive calcium channels mediate the entry of calcium into many types of excitable cells and thus play a key role in neurotransmitter release and excitation-contraction (E-C) coupling. The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type calcium channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. The DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling. The purified DHP receptor isolated from triads is composed of at least four subunits. The alpha-1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated sodium channel. The alpha-2 subunit is a large glycoprotein associated with the DHP receptor which was first described in skeletal muscle and is also found in high concentrations in other excitable tissues such as cardiac muscle and brain and in low concentrations in most other tissues studied. The other two subunits that co-purify with the DHP receptor are termed beta and gamma.
Immunogen :
Purified rabbit muscle T-tubule dihydropyridine receptor.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 1 µg/test, Immunohistochemistry (Frozen): 1:200, Immunohistochemistry (Paraffin): 1:20, Immunoprecipitation: Assay-dependent, Western Blot: 1:500
Aliases :
AW493108; CACH1; Cacn1; Cacna1s; Cacnl1a3; calcium channel, L type, alpha 1 polypeptide, isoform 3 (skeletal muscle, hypokalemic periodic paralysis); calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle; calcium channel, voltage-dependent, L type, alpha 1S subunit; calcium voltage-gated channel subunit alpha1 S; Cav1.1; Cav1.1 alpha-1; CCHL1A3; DHP; DHPR; DHPR alpha1s; dihydropyridine calcium channel receptor protein; dihydropyridine receptor; dihydropyridine receptor alpha 1 subunit; dihydropyridine receptor alpha 1S; dihydropyridine receptor alpha-1S subunit; dihydropyridine-sensitive L-type calcium channel alpha-1 subunit; fmd; HOKPP; HOKPP1; hypoPP; mdg; MHS5; muscle dysgenesis; ROB1; sj; TTPP1; unnamed protein product; voltage-dependent L-type calcium channel subunit alpha-1S; Voltage-gated calcium channel subunit alpha Cav1.1
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA