product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
SERCA2 ATPase Monoclonal Antibody (2A7-A1)
catalog :
MA3-919
quantity :
100 µL
price :
US 500.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2A7-A1
reactivity :
guinea pig, African green monkey, human, mouse, rat, dogs, pigs , fruit fly , domestic sheep, domestic rabbit
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 254
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:100; loading ...; fig 1e
Cuello F, Knaust A, Saleem U, Loos M, Raabe J, Mosqueira D, et al. Impairment of the ER/mitochondria compartment in human cardiomyocytes with PLN p.Arg14del mutation. EMBO Mol Med. 2021;13:e13074 pubmed publisher
  • western blot; African green monkey; 1:1000; loading ...; fig s11
Liu M, Li N, Qu C, Gao Y, Wu L, Hu L. Amylin deposition activates HIF1α and 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 (PFKFB3) signaling in failing hearts of non-human primates. Commun Biol. 2021;4:188 pubmed publisher
  • western blot; mouse; loading ...; fig 3g
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht M, et al. Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum. Neuron. 2021;109:299-313.e9 pubmed publisher
  • western blot; mouse; 1:2000; loading ...
Feng D, Xu D, Murakoshi N, Tajiri K, Qin R, Yonebayashi S, et al. Nicotinamide Phosphoribosyltransferase (Nampt)/Nicotinamide Adenine Dinucleotide (NAD) Axis Suppresses Atrial Fibrillation by Modulating the Calcium Handling Pathway. Int J Mol Sci. 2020;21: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4a
Dai W, Laforest B, Tyan L, Shen K, Nadadur R, Alvarado F, et al. A calcium transport mechanism for atrial fibrillation in Tbx5-mutant mice. elife. 2019;8: pubmed publisher
  • western blot; mouse; loading ...; fig 1g
Fajardo V, Chambers P, Juracic E, Rietze B, Gamu D, Bellissimo C, et al. Sarcolipin deletion in mdx mice impairs calcineurin signalling and worsens dystrophic pathology. Hum Mol Genet. 2018;27:4094-4102 pubmed publisher
  • western blot; mouse; loading ...; fig 7a
Tanwar V, Gorr M, Velten M, Eichenseer C, Long V, Bonilla I, et al. In Utero Particulate Matter Exposure Produces Heart Failure, Electrical Remodeling, and Epigenetic Changes at Adulthood. J Am Heart Assoc. 2017;6: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig s2f
Lee C, Hanna A, Wang H, Dagnino Acosta A, Joshi A, Knoblauch M, et al. A chemical chaperone improves muscle function in mice with a RyR1 mutation. Nat Commun. 2017;8:14659 pubmed publisher
  • western blot; mouse; loading ...; fig 2c
Fajardo V, Gamu D, Mitchell A, Bloemberg D, Bombardier E, Chambers P, et al. Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice. PLoS ONE. 2017;12:e0173708 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 3e
Abad M, Hashimoto H, Zhou H, Morales M, Chen B, Bassel Duby R, et al. Notch Inhibition Enhances Cardiac Reprogramming by Increasing MEF2C Transcriptional Activity. Stem Cell Reports. 2017;8:548-560 pubmed publisher
  • western blot; rat; loading ...; fig 2e
Himori K, Abe M, Tatebayashi D, Lee J, Westerblad H, Lanner J, et al. Superoxide dismutase/catalase mimetic EUK-134 prevents diaphragm muscle weakness in monocrotalin-induced pulmonary hypertension. PLoS ONE. 2017;12:e0169146 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2c
Fu Q, Hu Y, Wang Q, Liu Y, Li N, Xu B, et al. High-fat diet induces protein kinase A and G-protein receptor kinase phosphorylation of β2 -adrenergic receptor and impairs cardiac adrenergic reserve in animal hearts. J Physiol. 2017;595:1973-1986 pubmed publisher
  • western blot; mouse; loading ...; fig 10
Ramratnam M, Salama G, Sharma R, Wang D, Smith S, Banerjee S, et al. Gene-Targeted Mice with the Human Troponin T R141W Mutation Develop Dilated Cardiomyopathy with Calcium Desensitization. PLoS ONE. 2016;11:e0167681 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig 4a
  • western blot; mouse; 1:200; loading ...; fig 4c
Martin L, Horst K, Chiazza F, Oggero S, Collino M, Brandenburg K, et al. The synthetic antimicrobial peptide 19-2.5 attenuates septic cardiomyopathy and prevents down-regulation of SERCA2 in polymicrobial sepsis. Sci Rep. 2016;6:37277 pubmed publisher
  • western blot; mouse; fig 4c
Kennedy T, Swiderski K, Murphy K, Gehrig S, Curl C, Chandramouli C, et al. BGP-15 Improves Aspects of the Dystrophic Pathology in mdx and dko Mice with Differing Efficacies in Heart and Skeletal Muscle. Am J Pathol. 2016;186:3246-3260 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 1a
Helms A, Alvarado F, Yob J, Tang V, Pagani F, Russell M, et al. Genotype-Dependent and -Independent Calcium Signaling Dysregulation in Human Hypertrophic Cardiomyopathy. Circulation. 2016;134:1738-1748 pubmed
  • western blot; rat; fig 8
Hortemo K, Lunde P, Anonsen J, Kvaløy H, Munkvik M, Rehn T, et al. Exercise training increases protein O-GlcNAcylation in rat skeletal muscle. Physiol Rep. 2016;4: pubmed
  • western blot; human; 1:1000; fig s2
Mannhardt I, Breckwoldt K, Letuffe Brenière D, Schaaf S, Schulz H, Neuber C, et al. Human Engineered Heart Tissue: Analysis of Contractile Force. Stem Cell Reports. 2016;7:29-42 pubmed publisher
  • western blot; mouse; 1:2000; fig 1
Fajardo V, Smith I, Bombardier E, Chambers P, Quadrilatero J, Tupling A. Diaphragm assessment in mice overexpressing phospholamban in slow-twitch type I muscle fibers. Brain Behav. 2016;6:e00470 pubmed publisher
  • western blot; rat; 1:2500; fig s1
Sadredini M, Danielsen T, Aronsen J, Manotheepan R, Hougen K, Sjaastad I, et al. Beta-Adrenoceptor Stimulation Reveals Ca2+ Waves and Sarcoplasmic Reticulum Ca2+ Depletion in Left Ventricular Cardiomyocytes from Post-Infarction Rats with and without Heart Failure. PLoS ONE. 2016;11:e0153887 pubmed publisher
  • western blot; human; fig 3
Nakagawa T, Yokoe S, Asahi M. Phospholamban degradation is induced by phosphorylation-mediated ubiquitination and inhibited by interaction with cardiac type Sarco(endo)plasmic reticulum Ca(2+)-ATPase. Biochem Biophys Res Commun. 2016;472:523-30 pubmed publisher
  • western blot; mouse; 1:2000; loading ...; fig s8a
Nelson B, Makarewich C, Anderson D, Winders B, Troupes C, Wu F, et al. A peptide encoded by a transcript annotated as long noncoding RNA enhances SERCA activity in muscle. Science. 2016;351:271-5 pubmed publisher
  • western blot; domestic sheep; fig 1
Katz M, Brandon Warner E, Fargnoli A, Williams R, Kendle A, Hajjar R, et al. Mitigation of myocardial fibrosis by molecular cardiac surgery-mediated gene overexpression. J Thorac Cardiovasc Surg. 2016;151:1191-200.e3 pubmed publisher
  • western blot; rat; fig 2
Zhang H, Cannell M, Kim S, Watson J, Norman R, Calaghan S, et al. Cellular Hypertrophy and Increased Susceptibility to Spontaneous Calcium-Release of Rat Left Atrial Myocytes Due to Elevated Afterload. PLoS ONE. 2015;10:e0144309 pubmed publisher
  • western blot; mouse; fig 4
Ge W, Yuan M, Ceylan A, Wang X, Ren J. Mitochondrial aldehyde dehydrogenase protects against doxorubicin cardiotoxicity through a transient receptor potential channel vanilloid 1-mediated mechanism. Biochim Biophys Acta. 2016;1862:622-634 pubmed publisher
  • western blot; mouse
  • western blot; human
Fajardo V, Bombardier E, McMillan E, TRAN K, Wadsworth B, Gamu D, et al. Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype. Dis Model Mech. 2015;8:999-1009 pubmed publisher
  • western blot; rat; 1:1000; fig 5
Dalpiaz P, Lamas A, Caliman I, Ribeiro R, Abreu G, Moyses M, et al. Sex Hormones Promote Opposite Effects on ACE and ACE2 Activity, Hypertrophy and Cardiac Contractility in Spontaneously Hypertensive Rats. PLoS ONE. 2015;10:e0127515 pubmed publisher
  • western blot; mouse; fig 3.e,f
Yang L, Wang J, Yang J, Schamber R, Hu N, Nair S, et al. Antioxidant metallothionein alleviates endoplasmic reticulum stress-induced myocardial apoptosis and contractile dysfunction. Free Radic Res. 2015;49:1187-98 pubmed publisher
  • western blot; human
Tang Y, Yin Y, Miao L, Wei B, Zhai K, Ji G. Nitric oxide enhances extracellular ATP induced Ca²⁺ oscillation in HeLa cells. Arch Biochem Biophys. 2015;565:68-75 pubmed publisher
  • immunoprecipitation; domestic rabbit
  • western blot; domestic rabbit
Moshal K, Zhang Z, Roder K, Kim T, Cooper L, Patedakis Litvinov B, et al. Progesterone modulates SERCA2a expression and function in rabbit cardiomyocytes. Am J Physiol Cell Physiol. 2014;307:C1050-7 pubmed publisher
  • western blot; mouse; fig 6
Dweck D, Sanchez Gonzalez M, Chang A, Dulce R, Badger C, Koutnik A, et al. Long term ablation of protein kinase A (PKA)-mediated cardiac troponin I phosphorylation leads to excitation-contraction uncoupling and diastolic dysfunction in a knock-in mouse model of hypertrophic cardiomyopathy. J Biol Chem. 2014;289:23097-111 pubmed publisher
  • immunocytochemistry; mouse
  • immunohistochemistry; mouse
  • immunocytochemistry; domestic rabbit
  • immunohistochemistry; domestic rabbit
  • western blot; human
Ljubojević S, Radulovic S, Leitinger G, Sedej S, Sacherer M, Holzer M, et al. Early remodeling of perinuclear Ca2+ stores and nucleoplasmic Ca2+ signaling during the development of hypertrophy and heart failure. Circulation. 2014;130:244-55 pubmed publisher
  • western blot; mouse; 1:1000; fig 6f
Katanosaka Y, Iwasaki K, Ujihara Y, Takatsu S, Nishitsuji K, Kanagawa M, et al. TRPV2 is critical for the maintenance of cardiac structure and function in mice. Nat Commun. 2014;5:3932 pubmed publisher
  • western blot; rat; 1:1000
Mourouzis I, Mantzouratou P, Galanopoulos G, Kostakou E, Dhalla A, Belardinelli L, et al. The beneficial effects of ranolazine on cardiac function after myocardial infarction are greater in diabetic than in nondiabetic rats. J Cardiovasc Pharmacol Ther. 2014;19:457-69 pubmed publisher
  • western blot; mouse; 1:5000
Liu J, Sirenko S, Juhaszova M, Sollott S, Shukla S, Yaniv Y, et al. Age-associated abnormalities of intrinsic automaticity of sinoatrial nodal cells are linked to deficient cAMP-PKA-Ca(2+) signaling. Am J Physiol Heart Circ Physiol. 2014;306:H1385-97 pubmed publisher
  • western blot; human; 1:2000
Sivagangabalan G, Nazzari H, Bignolais O, Maguy A, Naud P, Farid T, et al. Regional ion channel gene expression heterogeneity and ventricular fibrillation dynamics in human hearts. PLoS ONE. 2014;9:e82179 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:1000; fig 4
  • immunoprecipitation; human; fig 6
  • western blot; human; 1:2000; fig 3
Fajardo V, Bombardier E, Vigna C, Devji T, Bloemberg D, Gamu D, et al. Co-expression of SERCA isoforms, phospholamban and sarcolipin in human skeletal muscle fibers. PLoS ONE. 2013;8:e84304 pubmed publisher
  • western blot; mouse
Groenke S, Larson E, Alber S, Zhang R, Lamp S, Ren X, et al. Complete atrial-specific knockout of sodium-calcium exchange eliminates sinoatrial node pacemaker activity. PLoS ONE. 2013;8:e81633 pubmed publisher
  • western blot; human; 1:1000
Kiyonaka S, Kajimoto T, Sakaguchi R, Shinmi D, Omatsu Kanbe M, Matsuura H, et al. Genetically encoded fluorescent thermosensors visualize subcellular thermoregulation in living cells. Nat Methods. 2013;10:1232-8 pubmed publisher
  • western blot; mouse; 1:2000
Fares E, Pyle W, Ray G, Rose R, Denovan Wright E, Chen R, et al. The impact of ovariectomy on calcium homeostasis and myofilament calcium sensitivity in the aging mouse heart. PLoS ONE. 2013;8:e74719 pubmed publisher
  • western blot; rat; 1:2000
Eshima H, Tanaka Y, Sonobe T, Inagaki T, Nakajima T, Poole D, et al. In vivo imaging of intracellular Ca2+ after muscle contractions and direct Ca2+ injection in rat skeletal muscle in diabetes. Am J Physiol Regul Integr Comp Physiol. 2013;305:R610-8 pubmed publisher
  • western blot; mouse; fig 7
Smith I, Bombardier E, Vigna C, Tupling A. ATP consumption by sarcoplasmic reticulum Ca²? pumps accounts for 40-50% of resting metabolic rate in mouse fast and slow twitch skeletal muscle. PLoS ONE. 2013;8:e68924 pubmed publisher
  • western blot; fruit fly
O Sullivan N, Dräger N, O Kane C. Characterization of the Drosophila atlastin interactome reveals VCP as a functionally related interactor. J Genet Genomics. 2013;40:297-306 pubmed publisher
  • western blot; mouse
Chang A, Huang J, Battiprolu P, Hill J, Kamm K, Stull J. The effects of neuregulin on cardiac Myosin light chain kinase gene-ablated hearts. PLoS ONE. 2013;8:e66720 pubmed publisher
  • western blot; mouse; 1:1000
Cheng Y, Wan X, McElfresh T, Chen X, Gresham K, Rosenbaum D, et al. Impaired contractile function due to decreased cardiac myosin binding protein C content in the sarcomere. Am J Physiol Heart Circ Physiol. 2013;305:H52-65 pubmed publisher
  • immunohistochemistry; rat; 1:500
Shortt C, Fredsted A, Bradford A, O Halloran K. Diaphragm muscle remodeling in a rat model of chronic intermittent hypoxia. J Histochem Cytochem. 2013;61:487-99 pubmed publisher
  • western blot; mouse; 1:1000
Mourouzis I, Kostakou E, Galanopoulos G, Mantzouratou P, Pantos C. Inhibition of thyroid hormone receptor ?1 impairs post-ischemic cardiac performance after myocardial infarction in mice. Mol Cell Biochem. 2013;379:97-105 pubmed publisher
  • western blot; human
Akimzhanov A, Barral J, Boehning D. Caspase 3 cleavage of the inositol 1,4,5-trisphosphate receptor does not contribute to apoptotic calcium release. Cell Calcium. 2013;53:152-8 pubmed publisher
  • western blot; human
Middlekauff H, Vigna C, Verity M, Fonarow G, Horwich T, Hamilton M, et al. Abnormalities of calcium handling proteins in skeletal muscle mirror those of the heart in humans with heart failure: a shared mechanism?. J Card Fail. 2012;18:724-33 pubmed publisher
  • immunohistochemistry; rat
Guglielmino K, Jackson K, Harris T, Vu V, Dong H, Dutrow G, et al. Pharmacological inhibition of soluble epoxide hydrolase provides cardioprotection in hyperglycemic rats. Am J Physiol Heart Circ Physiol. 2012;303:H853-62 pubmed publisher
  • western blot; rat
Sliwinska J, Dudka J, Korga A, Burdan F, Matysiak W, Jodlowska Jedrych B, et al. Tirapazamine-doxorubicin interaction referring to heart oxidative stress and Ca²? balance protein levels. Oxid Med Cell Longev. 2012;2012:890826 pubmed publisher
  • western blot; dogs
Moon M, Aziz A, Lee A, Moon C, Okada S, Kanter E, et al. Differential calcium handling in two canine models of right ventricular pressure overload. J Surg Res. 2012;178:554-62 pubmed publisher
  • western blot; rat
Korga A, Dudka J, Burdan F, Sliwinska J, Mandziuk S, Dawidek Pietryka K. The redox imbalance and the reduction of contractile protein content in rat hearts administered with L-thyroxine and Doxorubicin. Oxid Med Cell Longev. 2012;2012:681367 pubmed publisher
  • immunohistochemistry; mouse
  • western blot; mouse
Pinder J, Taylor Harris P, Bennett P, Carter E, Hayes N, King M, et al. Isoforms of protein 4.1 are differentially distributed in heart muscle cells: relation of 4.1R and 4.1G to components of the Ca2+ homeostasis system. Exp Cell Res. 2012;318:1467-79 pubmed publisher
  • western blot; domestic rabbit
Odening K, Choi B, Liu G, Hartmann K, Ziv O, Chaves L, et al. Estradiol promotes sudden cardiac death in transgenic long QT type 2 rabbits while progesterone is protective. Heart Rhythm. 2012;9:823-32 pubmed publisher
  • western blot; mouse; 1:750
Da Silva D, Costa D, Alves C, Block B, Landeira Fernandez A. Temperature dependence of cardiac sarcoplasmic reticulum Ca²?-ATPase from rainbow trout Oncorhynchus mykiss. J Fish Biol. 2011;79:789-800 pubmed publisher
  • western blot; rat
Garcia Perez C, Schneider T, Hajnoczky G, Csordás G. Alignment of sarcoplasmic reticulum-mitochondrial junctions with mitochondrial contact points. Am J Physiol Heart Circ Physiol. 2011;301:H1907-15 pubmed publisher
  • immunocytochemistry; mouse; 1:1000
Sztretye M, Yi J, Figueroa L, Zhou J, Royer L, Rios E. D4cpv-calsequestrin: a sensitive ratiometric biosensor accurately targeted to the calcium store of skeletal muscle. J Gen Physiol. 2011;138:211-29 pubmed publisher
  • western blot; human
De Marchi U, Castelbou C, Demaurex N. Uncoupling protein 3 (UCP3) modulates the activity of Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) by decreasing mitochondrial ATP production. J Biol Chem. 2011;286:32533-41 pubmed publisher
  • western blot; rat; 1:2000
Cheng Y, Li W, McElfresh T, Chen X, Berthiaume J, Castel L, et al. Changes in myofilament proteins, but not Ca²? regulation, are associated with a high-fat diet-induced improvement in contractile function in heart failure. Am J Physiol Heart Circ Physiol. 2011;301:H1438-46 pubmed publisher
  • western blot; mouse; 1:4000
Thomas M, Vigna C, Betik A, Tupling A, Hepple R. Cardiac calcium pump inactivation and nitrosylation in senescent rat myocardium are not attenuated by long-term treadmill training. Exp Gerontol. 2011;46:803-10 pubmed publisher
  • western blot; human
Lang D, Glukhov A, Efimova T, Efimov I. Role of Pyk2 in cardiac arrhythmogenesis. Am J Physiol Heart Circ Physiol. 2011;301:H975-83 pubmed publisher
  • western blot; mouse
Staunton L, Jockusch H, Wiegand C, Albrecht T, Ohlendieck K. Identification of secondary effects of hyperexcitability by proteomic profiling of myotonic mouse muscle. Mol Biosyst. 2011;7:2480-9 pubmed publisher
  • western blot; mouse; 1:1000
Coussee E, De Smet P, Bogaert E, Elens I, Van Damme P, Willems P, et al. G37R SOD1 mutant alters mitochondrial complex I activity, Ca(2+) uptake and ATP production. Cell Calcium. 2011;49:217-25 pubmed publisher
  • western blot; domestic sheep
Mariani J, Smolic A, Preovolos A, Byrne M, Power J, Kaye D. Augmentation of left ventricular mechanics by recirculation-mediated AAV2/1-SERCA2a gene delivery in experimental heart failure. Eur J Heart Fail. 2011;13:247-53 pubmed publisher
  • immunoprecipitation; mouse
  • western blot; mouse; 1:1000
Kamkin A, Kiseleva I, Theres H, Eulert Grehn J, Wagner K, Scholz H, et al. Enhanced L-type calcium currents in cardiomyocytes from transgenic rats overexpressing SERCA2a. Exp Clin Cardiol. 2010;15:e109-15 pubmed
  • western blot; mouse; 1:1000
Horiuchi Hirose M, Kashihara T, Nakada T, Kurebayashi N, Shimojo H, Shibazaki T, et al. Decrease in the density of t-tubular L-type Ca2+ channel currents in failing ventricular myocytes. Am J Physiol Heart Circ Physiol. 2011;300:H978-88 pubmed publisher
  • western blot; mouse
Hougen K, Aronsen J, Stokke M, Enger U, Nygard S, Andersson K, et al. Cre-loxP DNA recombination is possible with only minimal unspecific transcriptional changes and without cardiomyopathy in Tg(alphaMHC-MerCreMer) mice. Am J Physiol Heart Circ Physiol. 2010;299:H1671-8 pubmed publisher
  • western blot; rat; 1:1000
Kalofoutis C, Mourouzis I, Galanopoulos G, Dimopoulos A, Perimenis P, Spanou D, et al. Thyroid hormone can favorably remodel the diabetic myocardium after acute myocardial infarction. Mol Cell Biochem. 2010;345:161-9 pubmed publisher
  • western blot; mouse
Chandrasekharan K, Yoon J, Xu Y, DeVries S, Camboni M, Janssen P, et al. A human-specific deletion in mouse Cmah increases disease severity in the mdx model of Duchenne muscular dystrophy. Sci Transl Med. 2010;2:42ra54 pubmed publisher
  • immunocytochemistry; mouse
  • western blot; mouse
Jasuja R, Furie B, Furie B. Endothelium-derived but not platelet-derived protein disulfide isomerase is required for thrombus formation in vivo. Blood. 2010;116:4665-74 pubmed publisher
  • western blot; human; 1:3000
  • immunoprecipitation; rat
  • western blot; rat; 1:3000
Yokoe S, Asahi M, Takeda T, Otsu K, Taniguchi N, Miyoshi E, et al. Inhibition of phospholamban phosphorylation by O-GlcNAcylation: implications for diabetic cardiomyopathy. Glycobiology. 2010;20:1217-26 pubmed publisher
  • western blot; rat; 1:1000
Jeong M, Walker J, Brown R, Moore R, Vinson C, Colucci W, et al. AFos inhibits phenylephrine-mediated contractile dysfunction by altering phospholamban phosphorylation. Am J Physiol Heart Circ Physiol. 2010;298:H1719-26 pubmed publisher
  • western blot; rat; 1:4000
Thomas M, Vigna C, Betik A, Tupling A, Hepple R. Initiating treadmill training in late middle age offers modest adaptations in Ca2+ handling but enhances oxidative damage in senescent rat skeletal muscle. Am J Physiol Regul Integr Comp Physiol. 2010;298:R1269-78 pubmed publisher
  • immunohistochemistry; dogs; 1:1000
Summerfield N, Peters M, Hercock C, Mobasheri A, Young I. Immunohistochemical evidence for expression of fast-twitch type sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA1) in German shepherd dogs with dilated cardiomyopathy myocardium. J Vet Cardiol. 2010;12:17-23 pubmed publisher
  • western blot; mouse
Louch W, Hougen K, Mørk H, Swift F, Aronsen J, Sjaastad I, et al. Sodium accumulation promotes diastolic dysfunction in end-stage heart failure following Serca2 knockout. J Physiol. 2010;588:465-78 pubmed publisher
  • western blot; human
Munkvik M, Rehn T, Slettaløkken G, Hasic A, Hallen J, Sjaastad I, et al. Training effects on skeletal muscle calcium handling in human chronic heart failure. Med Sci Sports Exerc. 2010;42:847-55 pubmed publisher
  • western blot; mouse
Andersson K, Finsen A, Sjåland C, Winer L, Sjaastad I, Odegaard A, et al. Mice carrying a conditional Serca2(flox) allele for the generation of Ca(2+) handling-deficient mouse models. Cell Calcium. 2009;46:219-25 pubmed publisher
  • western blot; mouse
Ottenheijm C, Hidalgo C, Rost K, Gotthardt M, Granzier H. Altered contractility of skeletal muscle in mice deficient in titin's M-band region. J Mol Biol. 2009;393:10-26 pubmed publisher
  • western blot; rat
Pu M, Gao Z, Zhang X, Liao D, Pu D, Brennan T, et al. Impact of mitral regurgitation on left ventricular anatomic and molecular remodeling and systolic function: implication for outcome. Am J Physiol Heart Circ Physiol. 2009;296:H1727-32 pubmed publisher
  • western blot; mouse
Mørk H, Sjaastad I, Sejersted O, Louch W. Slowing of cardiomyocyte Ca2+ release and contraction during heart failure progression in postinfarction mice. Am J Physiol Heart Circ Physiol. 2009;296:H1069-79 pubmed publisher
  • immunohistochemistry; rat
Garcia Perez C, Hajnoczky G, Csordás G. Physical coupling supports the local Ca2+ transfer between sarcoplasmic reticulum subdomains and the mitochondria in heart muscle. J Biol Chem. 2008;283:32771-80 pubmed publisher
  • western blot; rat
O CONNELL K, Gannon J, Doran P, Ohlendieck K. Reduced expression of sarcalumenin and related Ca2+ -regulatory proteins in aged rat skeletal muscle. Exp Gerontol. 2008;43:958-61 pubmed publisher
  • western blot; mouse; 1:1,000
Ackermann G, Domenighetti A, Deten A, Bonath I, Marenholz I, Pedrazzini T, et al. S100A1 deficiency results in prolonged ventricular repolarization in response to sympathetic activation. Gen Physiol Biophys. 2008;27:127-42 pubmed
  • western blot; mouse
Palpant N, Day S, Herron T, Converso K, Metzger J. Single histidine-substituted cardiac troponin I confers protection from age-related systolic and diastolic dysfunction. Cardiovasc Res. 2008;80:209-18 pubmed publisher
  • western blot; rat; 1:1000
Mourouzis I, Dimopoulos A, Saranteas T, Tsinarakis N, Livadarou E, Spanou D, et al. Ischemic preconditioning fails to confer additional protection against ischemia-reperfusion injury in the hypothyroid rat heart. Physiol Res. 2009;58:29-38 pubmed
  • western blot; mouse
Bell J, Kennington E, Fuller W, Dighe K, Donoghue P, Clark J, et al. Characterization of the phospholemman knockout mouse heart: depressed left ventricular function with increased Na-K-ATPase activity. Am J Physiol Heart Circ Physiol. 2008;294:H613-21 pubmed
  • western blot; rat; 1:500
Feng Y, Dai D, Na T, Cui B, Wang T, Zhang Y, et al. Endothelin receptor antagonist CPU0213 suppresses ventricular fibrillation in L-thyroxin induced cardiomyopathy. Pharmacol Rep. 2007;59:306-14 pubmed
  • western blot; mouse
Mørk H, Sjaastad I, Sande J, Periasamy M, Sejersted O, Louch W. Increased cardiomyocyte function and Ca2+ transients in mice during early congestive heart failure. J Mol Cell Cardiol. 2007;43:177-86 pubmed
  • western blot; rat; 1:1,000
Pantos C, Mourouzis I, Markakis K, Dimopoulos A, Xinaris C, Kokkinos A, et al. Thyroid hormone attenuates cardiac remodeling and improves hemodynamics early after acute myocardial infarction in rats. Eur J Cardiothorac Surg. 2007;32:333-9 pubmed
  • western blot; rat; 1:500
Na T, Huang Z, Dai D, Zhang Y, Dai Y. Abrupt changes in FKBP12.6 and SERCA2a expression contribute to sudden occurrence of ventricular fibrillation on reperfusion and are prevented by CPU86017. Acta Pharmacol Sin. 2007;28:773-82 pubmed
  • western blot; rat
Na T, Dai D, Tang X, Dai Y. Upregulation of leptin pathway correlates with abnormal expression of SERCA2a, phospholamban and the endothelin pathway in heart failure and reversal by CPU86017. Naunyn Schmiedebergs Arch Pharmacol. 2007;375:39-49 pubmed
  • western blot; domestic rabbit; 1:1000
Walsh B, Naik H, Dubach J, Beshire M, Wieland A, Soybel D. Thiol-oxidant monochloramine mobilizes intracellular Ca2+ in parietal cells of rabbit gastric glands. Am J Physiol Cell Physiol. 2007;293:C1687-97 pubmed
  • immunocytochemistry; rat; 1:250
  • western blot; rat; 1:250
Prasad A, Ma H, Sumbilla C, Lee D, Klein M, Inesi G. Phenylephrine hypertrophy, Ca2+-ATPase (SERCA2), and Ca2+ signaling in neonatal rat cardiac myocytes. Am J Physiol Cell Physiol. 2007;292:C2269-75 pubmed
  • immunocytochemistry; mouse
  • western blot; mouse; 1:10,000
Stenoien D, Knyushko T, Londono M, Opresko L, Mayer M, Brady S, et al. Cellular trafficking of phospholamban and formation of functional sarcoplasmic reticulum during myocyte differentiation. Am J Physiol Cell Physiol. 2007;292:C2084-94 pubmed
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image
image 1 :
Invitrogen MA3-919 image 1
Immunofluorescent analysis of SERCA2 ATPase using SERCA2 ATPase Monoclonal antibody (2A7-A1) (Product# MA3-919) shows staining in U251 glioma cells. SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase (Product# MA3-919) at a dilution of 1:100-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
product information
Product Type :
Antibody
Product Name :
SERCA2 ATPase Monoclonal Antibody (2A7-A1)
Catalog # :
MA3-919
Quantity :
100 µL
Price :
US 500.00
Clonality :
Monoclonal
Host :
Mouse
Reactivity :
Canine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat
Applications :
Flow Cytometry: 1:20, Immunocytochemistry: 1:100-1:250, Immunohistochemistry (Frozen): 1/100, Immunohistochemistry (Paraffin): 1 µg/mL, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000
Species :
Canine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat
Clone :
2A7-A1
Isotype :
IgG2a
Storage :
-20° C, Avoid Freeze/Thaw Cycles
Description :
This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms.
Immunogen :
Purified canine cardiac sarcoplasmic reticulum vesicles.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 1:20, Immunocytochemistry: 1:100-1:250, Immunohistochemistry (Frozen): 1/100, Immunohistochemistry (Paraffin): 1 µg/mL, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000
Aliases :
9530097L16Rik; ATP2; Atp2a2; ATP2B; ATPase Ca++ transporting cardiac muscle slow twitch 2; ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2; ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2; ATPase, Ca++ transporting, cardiac muscle, slow twitch 2; ATPase, Ca++ transporting, slow twitch 2; Ca(2+)-transport ATPase class 3; calcium ATPase; calcium pump 2; calcium-ATPase (EC 3.6.1.3); calcium-transporting ATPase; calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; cardiac Ca2+ ATPase; D5Wsu150e; DAR; DD; endoplasmic reticulum class 1/2 Ca(2+) ATPase; mKIAA4195; putative SERCA isoform; sarco(endo)plasmic reticulum Ca(2+)-dependent ATPase 2; sarco/endoplasmic reticulum Ca2+-ATPase 2; sarcoplasmic reticulum Ca2+-transport ATPase isoform; sarcoplasmic/endoplasmic reticulum calcium ATPase 2; sarcoplasmic/endoplasmic-reticulum Ca(2+) pump gene 2; SERCA; SERCA ATPase; SERCA2; Serca2a; SERCA2B; SercaII; SR Ca(2+)-ATPase 2; unnamed protein product
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA