Calmodulin Monoclonal Antibody (2D1) | Invitrogen MA3-917 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Calmodulin Monoclonal Antibody (2D1)

catalog :

MA3-917

quantity :

200 µL

price :

US 375.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2D1

reactivity :

shrews, human, mouse, rat, chicken, bovine, fruit fly

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - free floating section, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; fig 5a	Huang M, Lee K, Kim K, Ahn M, Cho C, Kim D, et al. The maintenance ability and Ca2+ availability of skeletal muscle are enhanced by sildenafil. Exp Mol Med. 2016;48:e278 pubmed publisher
blocking or activating experiments; rat; 5 ug/ml	Greenlee J, Clawson S, Hill K, Wood B, Clardy S, Tsunoda I, et al. Anti-Yo antibody uptake and interaction with its intracellular target antigen causes Purkinje cell death in rat cerebellar slice cultures: a possible mechanism for paraneoplastic cerebellar degeneration in humans with gynecological or breast cancers. PLoS ONE. 2015;10:e0123446 pubmed publisher
immunohistochemistry - free floating section; shrews; 1:100	Zhong W, Hutchinson T, Chebolu S, Darmani N. Serotonin 5-HT3 receptor-mediated vomiting occurs via the activation of Ca2+/CaMKII-dependent ERK1/2 signaling in the least shrew (Cryptotis parva). PLoS ONE. 2014;9:e104718 pubmed publisher
western blot; fruit fly ; 1:500; fig s4	Galletta B, Guillen R, Fagerstrom C, Brownlee C, Lerit D, Megraw T, et al. Drosophila pericentrin requires interaction with calmodulin for its function at centrosomes and neuronal basal bodies but not at sperm basal bodies. Mol Biol Cell. 2014;25:2682-94 pubmed publisher
	Matthaeus C, Lian X, Kunz S, Lehmann M, Zhong C, Bernert C, et al. eNOS-NO-induced small blood vessel relaxation requires EHD2-dependent caveolae stabilization. PLoS ONE. 2019;14:e0223620 pubmed publisher
	Liu B, Zhou Y, Lu D, Liu Y, Zhang S, Xu Y, et al. Comparison of the protein expression of calpain-1, calpain-2, calpastatin and calmodulin between gastric cancer and normal gastric mucosa. Oncol Lett. 2017;14:3705-3710 pubmed publisher
	Ravel Chapuis A, Belanger G, Cote J, Michel R, Jasmin B. Misregulation of calcium-handling proteins promotes hyperactivation of calcineurin-NFAT signaling in skeletal muscle of DM1 mice. Hum Mol Genet. 2017;26:2192-2206 pubmed publisher
	Romani G, Bonza M, Filippini I, Cerana M, Beffagna N, De Michelis M. Involvement of the plasma membrane Ca2+-ATPase in the short-term response of Arabidopsis thaliana cultured cells to oligogalacturonides. Plant Biol (Stuttg). 2004;6:192-200 pubmed
	Zhu Q, Liu T, Clarke M. Calmodulin and the contractile vacuole complex in mitotic cells of Dictyostelium discoideum. J Cell Sci. 1993;104 ( Pt 4):1119-27 pubmed
	Hulen D, Baron A, Salisbury J, Clarke M. Production and specificity of monoclonal antibodies against calmodulin from Dictyostelium discoideum. Cell Motil Cytoskeleton. 1991;18:113-22 pubmed
	Coling D, Salisbury J. Characterization of the calcium-binding contractile protein centrin from Tetraselmis striata (Pleurastrophyceae). J Protozool. 1992;39:385-91 pubmed
	Zhu Q, Clarke M. Association of calmodulin and an unconventional myosin with the contractile vacuole complex of Dictyostelium discoideum. J Cell Biol. 1992;118:347-58 pubmed

product information

Product Type :

Antibody

Product Name :

Calmodulin Monoclonal Antibody (2D1)

Catalog # :

MA3-917

Quantity :

200 µL

Price :

US 375.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Bacteria, Bovine, Chicken, Human, Mouse, Rat

Applications :

ELISA: Assay-dependent, Flow Cytometry: 2 µg / 10^6 cells, Immunocytochemistry: 1:50, Immunohistochemistry: 1:20, Western Blot: 1:500

Species :

Bacteria, Bovine, Chicken, Human, Mouse, Rat

Clone :

2D1

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations. Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.

Immunogen :

Calmodulin purified from Dictyostelium discoideum.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Flow Cytometry: 2 µg / 10^6 cells, Immunocytochemistry: 1:50, Immunohistochemistry: 1:20, Western Blot: 1:500

Aliases :

1500001E21Rik; 2310037J09Rik; AI256814; AI327027; AI461935; AI649108; AL024000; AL024017; AY314008; calcium binding gene, DD112; calcium-binding protein Dd112; CALM; CALM 1; CALM1; CALM2; Calm3; Calm4; Calm5; CALML2; Calmodulin; calmodulin 1; calmodulin 1 (phosphorylase kinase, delta); calmodulin 2; calmodulin 2 (phosphorylase kinase, delta); calmodulin 3; calmodulin 3 (phosphorylase kinase, delta); calmodulin 4; calmodulin 5; Calmodulin III; calmodulin-1; Calmodulin-2; calmodulin-3; Calmodulin-4; calmodulin-like; calmodulin-like protein; calmodulin-like protein (neoCaM); caM; CAM1; Cam2; CAM3; CaMA; Camb; CamC; CaMI; CAMII; CAMIII; CPVT4; DD112; DD132; epididymis secretory protein Li 72; HEL-S-72; hypothetical protein; LP7057 protein; LQT14; LQT15; MGC128137 protein; neo-calmodulin; NeoCaM; PHKD; PHKD2; PHKD3; phosphorylase kinase delta; phosphorylase kinase subunit delta; phosphorylase kinase, delta subunit; prepro-calmodulin 1; prepro-calmodulin 2; prepro-calmodulin 3; R75142; RNCAMIII; Scarf; Scarf2; similar to calmodulin; skin calmodulin-related factor

more info or order :

Invitrogen product webpage