ATP1A3 Monoclonal Antibody (XVIF9-G10) | Invitrogen MA3-915 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

ATP1A3 Monoclonal Antibody (XVIF9-G10)

catalog :

MA3-915

quantity :

100 µg

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

XVIF9-G10

reactivity :

guinea pig, human, mouse, rat, dogs, bovine, pigs , domestic sheep, Xenopus laevis, domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 91

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:5000; loading ...; fig s2g	Smith S, Chen X, Brier L, Bumstead J, Rensing N, Ringel A, et al. Astrocyte deletion of α2-Na/K ATPase triggers episodic motor paralysis in mice via a metabolic pathway. Nat Commun. 2020;11:6164 pubmed publisher
immunohistochemistry; mouse; 1:500; loading ...; fig 2a	Vyas P, Wu J, Zimmerman A, Fuchs P, Glowatzki E. Tyrosine Hydroxylase Expression in Type II Cochlear Afferents in Mice. J Assoc Res Otolaryngol. 2017;18:139-151 pubmed publisher
western blot; human; 1:750; loading ...; fig 5c	Perry B, Wyckelsma V, Murphy R, Steward C, Anderson M, Levinger I, et al. Dissociation between short-term unloading and resistance training effects on skeletal muscle Na+,K+-ATPase, muscle function, and fatigue in humans. J Appl Physiol (1985). 2016;121:1074-1086 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; fig 1 western blot; mouse; 1:100	Su X, Tan Q, Parikh B, Tan A, Mehta M, Sia Wey Y, et al. Characterization of Fatty Acid Binding Protein 7 (FABP7) in the Murine Retina. Invest Ophthalmol Vis Sci. 2016;57:3397-408 pubmed publisher
immunocytochemistry; rat; fig 1	Blom H, Bernhem K, Brismar H. Sodium pump organization in dendritic spines. Neurophotonics. 2016;3:041803 pubmed publisher
western blot; human; 1:500; fig 1	Wyckelsma V, McKenna M, Levinger I, Petersen A, Lamboley C, Murphy R. Cell specific differences in the protein abundances of GAPDH and Na(+),K(+)-ATPase in skeletal muscle from aged individuals. Exp Gerontol. 2016;75:8-15 pubmed publisher
immunoprecipitation; bovine; fig 1a	Laird J, Pan Y, Modestou M, Yamaguchi D, Song H, Sokolov M, et al. Identification of a VxP Targeting Signal in the Flagellar Na+ /K+ -ATPase. Traffic. 2015;16:1239-53 pubmed publisher
western blot; human; fig 1	Akkuratov E, Wu J, Sowa D, Shah Z, Liu L. Ouabain-Induced Signaling and Cell Survival in SK-N-SH Neuroblastoma Cells Differentiated by Retinoic Acid. CNS Neurol Disord Drug Targets. 2015;14:1343-9 pubmed
western blot; human	Perry B, Levinger P, Morris H, Petersen A, Garnham A, Levinger I, et al. The effects of knee injury on skeletal muscle function, Na+, K+-ATPase content, and isoform abundance. Physiol Rep. 2015;3: pubmed publisher
immunocytochemistry; rat western blot; rat	Akkuratov E, Lopacheva O, KruusmÃ¤gi M, Lopachev A, Shah Z, Boldyrev A, et al. Functional Interaction Between Na/K-ATPase and NMDA Receptor in Cerebellar Neurons. Mol Neurobiol. 2015;52:1726-1734 pubmed publisher
western blot; human; 1 ug/ml	Roosterman D. Agonist-dependent and -independent dopamine-1-like receptor signalling differentially regulates downstream effectors. FEBS J. 2014;281:4792-804 pubmed publisher
western blot; rat	Schuth O, McLean W, Eatock R, Pyott S. Distribution of Na,K-ATPase α subunits in rat vestibular sensory epithelia. J Assoc Res Otolaryngol. 2014;15:739-54 pubmed publisher
western blot; human; 1:2000	Sivagangabalan G, Nazzari H, Bignolais O, Maguy A, Naud P, Farid T, et al. Regional ion channel gene expression heterogeneity and ventricular fibrillation dynamics in human hearts. PLoS ONE. 2014;9:e82179 pubmed publisher
western blot; Xenopus laevis; fig 2	Scholl E, Pirone A, Cox D, Duncan R, Jacob M. Alternative splice isoforms of small conductance calcium-activated SK2 channels differ in molecular interactions and surface levels. Channels (Austin). 2014;8:62-75 pubmed publisher
western blot; human	Perry B, Levinger P, Serpiello F, Caldow M, Cameron Smith D, Bartlett J, et al. The effects of osteoarthritis and age on skeletal muscle strength, Na+-K+-ATPase content, gene and isoform expression. J Appl Physiol (1985). 2013;115:1443-9 pubmed publisher
western blot; mouse; 1:500	Jansson K, Magenheimer B, Maser R, Calvet J, Blanco G. Overexpression of the polycystin-1 C-tail enhances sensitivity of M-1 cells to ouabain. J Membr Biol. 2013;246:581-90 pubmed publisher
immunohistochemistry; mouse; 1:1000	Edwards I, Bruce G, Lawrenson C, Howe L, Clapcote S, Deuchars S, et al. Na+/K+ ATPase α1 and α3 isoforms are differentially expressed in α- and γ-motoneurons. J Neurosci. 2013;33:9913-9 pubmed publisher
western blot; human	Xie C, Liu X, Yu S, Cheng C. Cardiac glycosides block cancer growth through HIF-1?- and NF-?B-mediated Plk1. Carcinogenesis. 2013;34:1870-80 pubmed publisher
immunocytochemistry; rat; 1:2000	Azarias G, Kruusmägi M, Connor S, Akkuratov E, Liu X, Lyons D, et al. A specific and essential role for Na,K-ATPase α3 in neurons co-expressing α1 and α3. J Biol Chem. 2013;288:2734-43 pubmed publisher
western blot; human	McKenna M, Perry B, Serpiello F, Caldow M, Levinger P, Cameron Smith D, et al. Unchanged [3H]ouabain binding site content but reduced Na+-K+ pump α2-protein abundance in skeletal muscle in older adults. J Appl Physiol (1985). 2012;113:1505-11 pubmed publisher
western blot; pigs ; 1:50000	Yang Z, Carter E, Kibler K, Kwansa H, Crafa D, Martin L, et al. Attenuation of neonatal ischemic brain damage using a 20-HETE synthesis inhibitor. J Neurochem. 2012;121:168-79 pubmed publisher
immunocytochemistry; mouse; 1:100 western blot; mouse; 1:100	Xu Y, Dhingra A, Fina M, Koike C, Furukawa T, Vardi N. mGluR6 deletion renders the TRPM1 channel in retina inactive. J Neurophysiol. 2012;107:948-57 pubmed publisher
western blot; human	Petersen A, Leikis M, McMahon L, Kent A, Murphy K, Gong X, et al. Impaired exercise performance and muscle Na(+),K(+)-pump activity in renal transplantation and haemodialysis patients. Nephrol Dial Transplant. 2012;27:2036-43 pubmed publisher
western blot; mouse	Dunn D, He D, Yang P, Johansen M, Newman R, Lo D. In vitro and in vivo neuroprotective activity of the cardiac glycoside oleandrin from Nerium oleander in brain slice-based stroke models. J Neurochem. 2011;119:805-14 pubmed publisher
immunocytochemistry; mouse	Blom H, Rönnlund D, Scott L, Spicarova Z, Rantanen V, Widengren J, et al. Nearest neighbor analysis of dopamine D1 receptors and Na(+)-K(+)-ATPases in dendritic spines dissected by STED microscopy. Microsc Res Tech. 2012;75:220-8 pubmed publisher
western blot; mouse	Leo L, Gherardini L, Barone V, De Fusco M, Pietrobon D, Pizzorusso T, et al. Increased susceptibility to cortical spreading depression in the mouse model of familial hemiplegic migraine type 2. PLoS Genet. 2011;7:e1002129 pubmed publisher
immunocytochemistry; mouse	Blom H, Rönnlund D, Scott L, Spicarova Z, Widengren J, Bondar A, et al. Spatial distribution of Na+-K+-ATPase in dendritic spines dissected by nanoscale superresolution STED microscopy. BMC Neurosci. 2011;12:16 pubmed publisher
western blot; rat; 1 ug/ml	Bersier M, Peña C, Arnaiz G. Changes in Na+, K+-ATPase activity and alpha 3 subunit expression in CNS after administration of Na+, K+-ATPase inhibitors. Neurochem Res. 2011;36:297-303 pubmed publisher
immunohistochemistry; rat; 1:600	Wersinger E, McLean W, Fuchs P, Pyott S. BK channels mediate cholinergic inhibition of high frequency cochlear hair cells. PLoS ONE. 2010;5:e13836 pubmed publisher
western blot; human	Phakdeekitcharoen B, Kittikanokrat W, Kijkunasathian C, Chatsudthipong V. Aldosterone increases Na+ -K+ -ATPase activity in skeletal muscle of patients with Conn's syndrome. Clin Endocrinol (Oxf). 2011;74:152-9 pubmed publisher
western blot; mouse; 1:1000	Oshiro N, Dostanic Larson I, Neumann J, Lingrel J. The ouabain-binding site of the ?2 isoform of Na,K-ATPase plays a role in blood pressure regulation during pregnancy. Am J Hypertens. 2010;23:1279-85 pubmed publisher
immunocytochemistry; rat western blot; rat	Parekh A, Campbell A, Djouhri L, Fang X, McMullan S, Berry C, et al. Immunostaining for the ?3 isoform of the Na+/K+-ATPase is selective for functionally identified muscle spindle afferents in vivo. J Physiol. 2010;588:4131-43 pubmed publisher
western blot; mouse	Quintas L, Pierre S, Liu L, Bai Y, Liu X, Xie Z. Alterations of Na+/K+-ATPase function in caveolin-1 knockout cardiac fibroblasts. J Mol Cell Cardiol. 2010;49:525-31 pubmed publisher
western blot; human; 1:1000	Shibuya K, Fukuoka J, Fujii T, Shimoda E, Shimizu T, Sakai H, et al. Increase in ouabain-sensitive K+-ATPase activity in hepatocellular carcinoma by overexpression of Na+, K+-ATPase alpha 3-isoform. Eur J Pharmacol. 2010;638:42-6 pubmed publisher
immunocytochemistry; mouse; 1:500 western blot; mouse	Valencia A, Reeves P, Sapp E, Li X, Alexander J, Kegel K, et al. Mutant huntingtin and glycogen synthase kinase 3-beta accumulate in neuronal lipid rafts of a presymptomatic knock-in mouse model of Huntington's disease. J Neurosci Res. 2010;88:179-90 pubmed publisher
immunoprecipitation; rat western blot; rat; 1:2000	Rose E, Koo J, Antflick J, Ahmed S, Angers S, Hampson D. Glutamate transporter coupling to Na,K-ATPase. J Neurosci. 2009;29:8143-55 pubmed publisher
western blot; human	Kim M, Lyu J, Sohn K, Kim M, Cho M, Joo C, et al. Over-expression of translationally controlled tumor protein in lens epithelial cells seems to be associated with cataract development. Transgenic Res. 2009;18:953-60 pubmed publisher
immunohistochemistry; rat; 1:500	McLean W, Smith K, Glowatzki E, Pyott S. Distribution of the Na,K-ATPase alpha subunit in the rat spiral ganglion and organ of corti. J Assoc Res Otolaryngol. 2009;10:37-49 pubmed publisher
western blot; human	Ghosh B, Kar P, Mandal A, Dey K, Chakraborti T, Chakraborti S. Ca2+ influx mechanisms in caveolae vesicles of pulmonary smooth muscle plasma membrane under inhibition of alpha2beta1 isozyme of Na+/K+-ATPase by ouabain. Life Sci. 2009;84:139-48 pubmed publisher
western blot; bovine immunohistochemistry; mouse; 1:200 western blot; mouse	Molday L, Wu W, Molday R. Retinoschisin (RS1), the protein encoded by the X-linked retinoschisis gene, is anchored to the surface of retinal photoreceptor and bipolar cells through its interactions with a Na/K ATPase-SARM1 complex. J Biol Chem. 2007;282:32792-801 pubmed
western blot; human	Aughey R, Murphy K, Clark S, Garnham A, Snow R, Cameron Smith D, et al. Muscle Na+-K+-ATPase activity and isoform adaptations to intense interval exercise and training in well-trained athletes. J Appl Physiol (1985). 2007;103:39-47 pubmed
western blot; human	Nguyen A, Wallace D, Blanco G. Ouabain binds with high affinity to the Na,K-ATPase in human polycystic kidney cells and induces extracellular signal-regulated kinase activation and cell proliferation. J Am Soc Nephrol. 2007;18:46-57 pubmed
immunocytochemistry; human; 1:1000	Valencia A, Rajadurai A, Carle A, Kochevar I. 7-Dehydrocholesterol enhances ultraviolet A-induced oxidative stress in keratinocytes: roles of NADPH oxidase, mitochondria, and lipid rafts. Free Radic Biol Med. 2006;41:1704-18 pubmed
western blot; mouse	Martin L, Liu Z, Chen K, Price A, Pan Y, Swaby J, et al. Motor neuron degeneration in amyotrophic lateral sclerosis mutant superoxide dismutase-1 transgenic mice: mechanisms of mitochondriopathy and cell death. J Comp Neurol. 2007;500:20-46 pubmed
western blot; human; 1:500	Sanchez G, Nguyen A, Timmerberg B, Tash J, Blanco G. The Na,K-ATPase alpha4 isoform from humans has distinct enzymatic properties and is important for sperm motility. Mol Hum Reprod. 2006;12:565-76 pubmed
immunoprecipitation; pigs immunocytochemistry; pigs ; 1:100 western blot; pigs ; 1:4000	Harada K, Lin H, Endo Y, Fujishiro N, Sakamoto Y, Inoue M. Subunit composition and role of Na+,K+-ATPases in ventricular myocytes. J Physiol Sci. 2006;56:113-21 pubmed
western blot; human; 1:10,000	Murphy K, Petersen A, Goodman C, Gong X, Leppik J, Garnham A, et al. Prolonged submaximal exercise induces isoform-specific Na+-K+-ATPase mRNA and protein responses in human skeletal muscle. Am J Physiol Regul Integr Comp Physiol. 2006;290:R414-24 pubmed
western blot; human	Kimura M, Cao X, Aviv A. Calcium adaptation to sodium pump inhibition in a human megakaryocytic cell line. Am J Physiol Cell Physiol. 2005;289:C891-7 pubmed
western blot; rat	Xu K, Takimoto E, Juang G, Zhang Q, Rohde H, Myers A. Evidence that the H1-H2 domain of alpha1 subunit of (Na++K+)-ATPase participates in the regulation of cardiac contraction. FASEB J. 2005;19:53-61 pubmed
	Plössl K, Straub K, Schmid V, Strunz F, Wild J, Merkl R, et al. Identification of the retinoschisin-binding site on the retinal Na/K-ATPase. PLoS ONE. 2019;14:e0216320 pubmed publisher
	Vyas P, Wu J, Jimenez A, Glowatzki E, Fuchs P. Characterization of transgenic mouse lines for labeling type I and type II afferent neurons in the cochlea. Sci Rep. 2019;9:5549 pubmed publisher
	Sharma K, Seo Y, Yi E. Differential Expression of Ca2+-buffering Protein Calretinin in Cochlear Afferent Fibers: A Possible Link to Vulnerability to Traumatic Noise. Exp Neurobiol. 2018;27:397-407 pubmed publisher
	Furutani Y, Yoshihara Y. Proteomic Analysis of Dendritic Filopodia-Rich Fraction Isolated by Telencephalin and Vitronectin Interaction. Front Synaptic Neurosci. 2018;10:27 pubmed publisher
	Liebmann T, Fritz N, Kruusmägi M, Westin L, Bernhem K, Bondar A, et al. Regulation of Neuronal Na,K-ATPase by Extracellular Scaffolding Proteins. Int J Mol Sci. 2018;19: pubmed publisher
	Floyd R, Mobasheri A, Wray S. Gestation changes sodium pump isoform expression, leading to changes in ouabain sensitivity, contractility, and intracellular calcium in rat uterus. Physiol Rep. 2017;5: pubmed publisher
	Plössl K, Royer M, Bernklau S, Tavraz N, Friedrich T, Wild J, et al. Retinoschisin is linked to retinal Na/K-ATPase signaling and localization. Mol Biol Cell. 2017;28:2178-2189 pubmed publisher
	Wyckelsma V, McKenna M, Serpiello F, Lamboley C, Aughey R, Stepto N, et al. Single-fiber expression and fiber-specific adaptability to short-term intense exercise training of Na+-K+-ATPase Î±- and Î²-isoforms in human skeletal muscle. J Appl Physiol (1985). 2015;118:699-706 pubmed publisher
	Ramamurthy V, Jolicoeur C, Koutroumbas D, MÃ¼hlhans J, Le Y, Hauswirth W, et al. Numb regulates the polarized delivery of cyclic nucleotide-gated ion channels in rod photoreceptor cilia. J Neurosci. 2014;34:13976-87 pubmed publisher
	Paul D, Soignier R, Minor L, Tau H, Songu Mize E, Gould H. Regulation and pharmacological blockade of sodium-potassium ATPase: a novel pathway to neuropathy. J Neurol Sci. 2014;340:139-43 pubmed publisher
	Hou X, Theriault S, Dostanic Larson I, Moseley A, Lingrel J, Wu H, et al. Enhanced pressor response to increased CSF sodium concentration and to central ANG I in heterozygous alpha2 Na+ -K+ -ATPase knockout mice. Am J Physiol Regul Integr Comp Physiol. 2009;296:R1427-38 pubmed publisher
	Dora K, Gallagher N, McNeish A, Garland C. Modulation of endothelial cell KCa3.1 channels during endothelium-derived hyperpolarizing factor signaling in mesenteric resistance arteries. Circ Res. 2008;102:1247-55 pubmed publisher
	Moseley A, Williams M, Schaefer T, Bohanan C, Neumann J, Behbehani M, et al. Deficiency in Na,K-ATPase alpha isoform genes alters spatial learning, motor activity, and anxiety in mice. J Neurosci. 2007;27:616-26 pubmed
	Lunde P, Sejersted O, Thorud H, Tønnessen T, Henriksen U, Christensen G, et al. Effects of congestive heart failure on Ca2+ handling in skeletal muscle during fatigue. Circ Res. 2006;98:1514-9 pubmed
	Dostanic I, Schultz J, Lorenz J, Lingrel J. The alpha 1 isoform of Na,K-ATPase regulates cardiac contractility and functionally interacts and co-localizes with the Na/Ca exchanger in heart. J Biol Chem. 2004;279:54053-61 pubmed
	de Carvalho Aguiar P, Sweadner K, Penniston J, Zaremba J, Liu L, Caton M, et al. Mutations in the Na+/K+ -ATPase alpha3 gene ATP1A3 are associated with rapid-onset dystonia parkinsonism. Neuron. 2004;43:169-75 pubmed
	Radzyukevich T, Moseley A, Shelly D, Redden G, Behbehani M, Lingrel J, et al. The Na(+)-K(+)-ATPase alpha2-subunit isoform modulates contractility in the perinatal mouse diaphragm. Am J Physiol Cell Physiol. 2004;287:C1300-10 pubmed
	Murphy K, Snow R, Petersen A, Murphy R, Mollica J, Lee J, et al. Intense exercise up-regulates Na+,K+-ATPase isoform mRNA, but not protein expression in human skeletal muscle. J Physiol. 2004;556:507-19 pubmed
	Hansen O. Quantification of alpha-subunit isoforms of Na,K-ATPase in rat resistance vessels. Acta Physiol Scand. 2004;180:49-56 pubmed
	Shelly D, He S, Moseley A, Weber C, Stegemeyer M, Lynch R, et al. Na(+) pump alpha 2-isoform specifically couples to contractility in vascular smooth muscle: evidence from gene-targeted neonatal mice. Am J Physiol Cell Physiol. 2004;286:C813-20 pubmed
	Ferguson S, Savchenko V, Apparsundaram S, Zwick M, Wright J, Heilman C, et al. Vesicular localization and activity-dependent trafficking of presynaptic choline transporters. J Neurosci. 2003;23:9697-709 pubmed
	Dostanic I, Lorenz J, Schultz J, Grupp I, Neumann J, Wani M, et al. The alpha2 isoform of Na,K-ATPase mediates ouabain-induced cardiac inotropy in mice. J Biol Chem. 2003;278:53026-34 pubmed
	Wetzel R, Sweadner K. Phospholemman expression in extraglomerular mesangium and afferent arteriole of the juxtaglomerular apparatus. Am J Physiol Renal Physiol. 2003;285:F121-9 pubmed
	Dobretsov M, Hastings S, Sims T, Stimers J, Romanovsky D. Stretch receptor-associated expression of alpha 3 isoform of the Na+, K+-ATPase in rat peripheral nervous system. Neuroscience. 2003;116:1069-80 pubmed
	Moseley A, Lieske S, Wetzel R, James P, He S, Shelly D, et al. The Na,K-ATPase alpha 2 isoform is expressed in neurons, and its absence disrupts neuronal activity in newborn mice. J Biol Chem. 2003;278:5317-24 pubmed
	Hoffman J, Wickrema A, Potapova O, Milanick M, Yingst D. Na pump isoforms in human erythroid progenitor cells and mature erythrocytes. Proc Natl Acad Sci U S A. 2002;99:14572-7 pubmed
	Brickley D, Mikosz C, Hagan C, Conzen S. Ubiquitin modification of serum and glucocorticoid-induced protein kinase-1 (SGK-1). J Biol Chem. 2002;277:43064-70 pubmed
	Cui G, Dean W, Delamere N. The influence of cycloheximide on Na,K-ATPase activity in cultured human lens epithelial cells. Invest Ophthalmol Vis Sci. 2002;43:2714-20 pubmed
	Moseley A, Graw J, Delamere N. Altered Na,K-ATPase pattern in gamma-crystallin mutant mice. Invest Ophthalmol Vis Sci. 2002;43:1517-9 pubmed
	Fedorova O, Dorofeeva N, Lopatin D, Lakatta E, Bagrov A. Phorbol diacetate potentiates na(+)-k(+) ATPase inhibition by a putative endogenous ligand, marinobufagenin. Hypertension. 2002;39:298-302 pubmed
	He S, Shelly D, Moseley A, James P, James J, Paul R, et al. The alpha(1)- and alpha(2)-isoforms of Na-K-ATPase play different roles in skeletal muscle contractility. Am J Physiol Regul Integr Comp Physiol. 2001;281:R917-25 pubmed
	Kong J, Meng J, Biser P, Fleming W, Taylor D. Cellular depolarization of neurons in the locus ceruleus region of the guinea pig associated with the development of tolerance to opioids. J Pharmacol Exp Ther. 2001;298:909-16 pubmed
	Sencer S, Papineni R, Halling D, Pate P, Krol J, Zhang J, et al. Coupling of RYR1 and L-type calcium channels via calmodulin binding domains. J Biol Chem. 2001;276:38237-41 pubmed
	Wetzel R, Sweadner K. Immunocytochemical localization of NaK-ATPase isoforms in the rat and mouse ocular ciliary epithelium. Invest Ophthalmol Vis Sci. 2001;42:763-9 pubmed
	Fedorova O, Lakatta E, Bagrov A. Endogenous Na,K pump ligands are differentially regulated during acute NaCl loading of Dahl rats. Circulation. 2000;102:3009-14 pubmed
	Biser P, Thayne K, Kong J, Fleming W, Taylor D. Quantification of the alpha(3) subunit of the Na(+)/K(+)-ATPase in developing rat cerebellum. Brain Res Dev Brain Res. 2000;123:165-72 pubmed
	Tao Q, Hollenberg N, Graves S. Sodium pump inhibition and regional expression of sodium pump alpha-isoforms in lens. Hypertension. 1999;34:1168-74 pubmed
	James P, Grupp I, Grupp G, Woo A, Askew G, Croyle M, et al. Identification of a specific role for the Na,K-ATPase alpha 2 isoform as a regulator of calcium in the heart. Mol Cell. 1999;3:555-63 pubmed
	Pfeiffer R, Beron J, Verrey F. Regulation of Na+ pump function by aldosterone is alpha-subunit isoform specific. J Physiol. 1999;516 ( Pt 3):647-55 pubmed
	Hansen O. Heterogeneity of Na+/K+-ATPase from rectal gland of Squalus acanthias is not due to alpha isoform diversity. Pflugers Arch. 1999;437:517-22 pubmed
	Peng L, Martin Vasallo P, Sweadner K. Isoforms of Na,K-ATPase alpha and beta subunits in the rat cerebellum and in granule cell cultures. J Neurosci. 1997;17:3488-502 pubmed
	Arystarkhova E, Sweadner K. Isoform-specific monoclonal antibodies to Na,K-ATPase alpha subunits. Evidence for a tissue-specific post-translational modification of the alpha subunit. J Biol Chem. 1996;271:23407-17 pubmed

image

image 1 :

Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) (Product# MA3-915) shows staining in C6 glioma cells. Sodium/Potassium ATPase alpha-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 (Product# MA3-915) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

ATP1A3 Monoclonal Antibody (XVIF9-G10)

Catalog # :

MA3-915

Quantity :

100 µg

Price :

US 399.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Bovine, Canine, Guinea Pig, Human, Mouse, Non-human primate, Ovine, Rabbit, Rat

Applications :

Flow Cytometry: 1 µg / 10^6 cells, Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Frozen): 3 µg/mL, Western Blot: 1 µg/mL

Species :

Bovine, Canine, Guinea Pig, Human, Mouse, Non-human primate, Ovine, Rabbit, Rat

Clone :

XVIF9-G10

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The alpha-3 subunit has been found in neuronal and to skeletal and cardiac muscle, lung and stomach tissues.

Immunogen :

Canine cardiac microsomes.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1 µg / 10^6 cells, Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Frozen): 3 µg/mL, Western Blot: 1 µg/mL

Aliases :

AHC2; ATP1A1; Atp1a3; ATPa1a3; Atpa-2; ATPase Na+/K+ transporting subunit alpha 3; ATPase, Na+/K+ transporting, alpha 3 polypeptide; ATPase, Na+K+ transporting, alpha 3 subunit; CAPOS; DYT12; EC 3.6.3.9; Na(+)/K(+) ATPase alpha(III) subunit; Na(+)/K(+) ATPase alpha-3 subunit; Na+, K+ activated adenosine triphosphatase alpha subunit; Na+/K+ ATPase 3; Na+/K+ -ATPase alpha 3 subunit; RDP; Sodium Potassium ATPase; Sodium pump 3; sodium pump subunit alpha-3; sodium/potassium-transporting ATPase alpha-3 chain; sodium/potassium-transporting ATPase subunit alpha-3; sodium-potassium ATPase catalytic subunit alpha-3; sodium-potassium-ATPase, alpha 3 polypeptide

more info or order :

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