PMCA ATPase Monoclonal Antibody (5F10) | Invitrogen MA3-914 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PMCA ATPase Monoclonal Antibody (5F10)

catalog :

MA3-914

quantity :

100 µL

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5F10

reactivity :

Pacific electric ray, African green monkey, human, mouse, rat, dogs, chicken, bovine, pigs , cat, domestic sheep, Xenopus laevis, domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 115

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:2000; loading ...; fig 3s1	Abildgaard A, Stein A, Nielsen S, Schultz Knudsen K, Papaleo E, Shrikhande A, et al. Computational and cellular studies reveal structural destabilization and degradation of MLH1 variants in Lynch syndrome. elife. 2019;8: pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500; loading ...; tbl 2	Chaney S, Mukherjee S, Giddabasappa A, Rueda E, Hamilton W, Johnson J, et al. Increased proliferation of late-born retinal progenitor cells by gestational lead exposure delays rod and bipolar cell differentiation. Mol Vis. 2016;22:1468-1489 pubmed
western blot; mouse; loading ...; fig 3j	PÃ©rez CaÃ±amÃ¡s A, BenvegnÃ¹ S, Rueda C, RÃ¡bano A, SatrÃºstegui J, Ledesma M. Sphingomyelin-induced inhibition of the plasma membrane calcium ATPase causes neurodegeneration in type A Niemann-Pick disease. Mol Psychiatry. 2017;22:711-723 pubmed publisher
western blot; mouse; loading ...; fig 1a	Albarrán L, Regodón S, Salido G, Lopez J, Rosado J. Role of STIM1 in the surface expression of SARAF. Channels (Austin). 2017;11:84-88 pubmed publisher
immunoprecipitation; pigs ; fig 9 western blot; pigs ; fig 9	Tran Q, Firkins R, Giles J, Francis S, Matnishian V, Tran P, et al. Estrogen Enhances Linkage in the Vascular Endothelial Calmodulin Network via a Feedforward Mechanism at the G Protein-coupled Estrogen Receptor 1. J Biol Chem. 2016;291:10805-23 pubmed publisher
western blot; mouse; 1:250; loading ...; fig s8a	Nelson B, Makarewich C, Anderson D, Winders B, Troupes C, Wu F, et al. A peptide encoded by a transcript annotated as long noncoding RNA enhances SERCA activity in muscle. Science. 2016;351:271-5 pubmed publisher
immunohistochemistry; mouse; 1:500; loading ...; fig s5	Valenza M, Chen J, Di Paolo E, Ruozi B, Belletti D, Ferrari Bardile C, et al. Cholesterol-loaded nanoparticles ameliorate synaptic and cognitive function in Huntington's disease mice. EMBO Mol Med. 2015;7:1547-64 pubmed publisher
western blot; human; 1:1000; fig 1	Sharma P, Abbasi C, Lazic S, Teng A, Wang D, Dubois N, et al. Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function. Nat Commun. 2015;6:8391 pubmed publisher
immunocytochemistry; human; 1:100 western blot; human; 1:1000	Cali T, Lopreiato R, Shimony J, Vineyard M, Frizzarin M, Zanni G, et al. A Novel Mutation in Isoform 3 of the Plasma Membrane Ca2+ Pump Impairs Cellular Ca2+ Homeostasis in a Patient with Cerebellar Ataxia and Laminin Subunit 1α Mutations. J Biol Chem. 2015;290:16132-41 pubmed publisher
immunoprecipitation; human; fig 7 immunocytochemistry; human; fig 3 western blot; human; fig 2	Tran Q, Vermeer M, Burgard M, Hassan A, Giles J. Hetero-oligomeric Complex between the G Protein-coupled Estrogen Receptor 1 and the Plasma Membrane Ca2+-ATPase 4b. J Biol Chem. 2015;290:13293-307 pubmed publisher
immunoprecipitation; human; 1:500 western blot; human; 1:1000	Philippe R, Antigny F, Buscaglia P, Norez C, Becq F, Frieden M, et al. SERCA and PMCA pumps contribute to the deregulation of Ca2+ homeostasis in human CF epithelial cells. Biochim Biophys Acta. 2015;1853:892-903 pubmed publisher
immunohistochemistry - paraffin section; human; 1:1000 western blot; human; 1:1000	Abad C, Vallejos C, de Gregorio N, DÃaz P, Chiarello D, Mendoza M, et al. Naâº, Kâº-ATPase and CaÂ²âº-ATPase activities in basal and microvillous syncytiotrophoblast membranes from preeclamptic human term placenta. Hypertens Pregnancy. 2015;34:65-79 pubmed publisher
immunoprecipitation; rat western blot; rat	de Juan Sanz J, NÃºÃ±ez E, Zafra F, Berrocal M, Corbacho I, IbÃ¡Ã±ez I, et al. Presynaptic control of glycine transporter 2 (GlyT2) by physical and functional association with plasma membrane Ca2+-ATPase (PMCA) and Na+-Ca2+ exchanger (NCX). J Biol Chem. 2014;289:34308-24 pubmed publisher
western blot; mouse	Albarrán L, Berna Erro A, Dionisio N, Redondo P, Lopez E, Lopez J, et al. TRPC6 participates in the regulation of cytosolic basal calcium concentration in murine resting platelets. Biochim Biophys Acta. 2014;1843:789-96 pubmed publisher
western blot; mouse	Groenke S, Larson E, Alber S, Zhang R, Lamp S, Ren X, et al. Complete atrial-specific knockout of sodium-calcium exchange eliminates sinoatrial node pacemaker activity. PLoS ONE. 2013;8:e81633 pubmed publisher
western blot; African green monkey	Küch E, Vellaramkalayil R, Zhang I, Lehnen D, Brugger B, Sreemmel W, et al. Differentially localized acyl-CoA synthetase 4 isoenzymes mediate the metabolic channeling of fatty acids towards phosphatidylinositol. Biochim Biophys Acta. 2014;1841:227-39 pubmed
immunoprecipitation; human	Albarrán L, Dionisio N, Lopez E, Salido G, Rosado J. The membrane potential modulates thrombin-stimulated Ca²? mobilization and platelet aggregation. Arch Biochem Biophys. 2013;538:130-7 pubmed publisher
western blot; rat; 1:80	Dauner K, Möbus C, Frings S, Möhrlen F. Targeted expression of anoctamin calcium-activated chloride channels in rod photoreceptor terminals of the rodent retina. Invest Ophthalmol Vis Sci. 2013;54:3126-36 pubmed publisher
immunocytochemistry; human; 1:100	Zanni G, Cali T, Kalscheuer V, Ottolini D, Barresi S, Lebrun N, et al. Mutation of plasma membrane Ca2+ ATPase isoform 3 in a family with X-linked congenital cerebellar ataxia impairs Ca2+ homeostasis. Proc Natl Acad Sci U S A. 2012;109:14514-9 pubmed publisher
western blot; mouse	Leanza L, Zoratti M, Gulbins E, Szabo I. Induction of apoptosis in macrophages via Kv1.3 and Kv1.5 potassium channels. Curr Med Chem. 2012;19:5394-404 pubmed
western blot; human; 1:2000	Curry M, Luk N, Kenny P, Roberts Thomson S, Monteith G. Distinct regulation of cytoplasmic calcium signals and cell death pathways by different plasma membrane calcium ATPase isoforms in MDA-MB-231 breast cancer cells. J Biol Chem. 2012;287:28598-608 pubmed publisher
immunohistochemistry; rat; 1:400	Chen Q, Mahendrasingam S, Tickle J, Hackney C, Furness D, Fettiplace R. The development, distribution and density of the plasma membrane calcium ATPase 2 calcium pump in rat cochlear hair cells. Eur J Neurosci. 2012;36:2302-10 pubmed publisher
western blot; human	Leanza L, Henry B, Sassi N, Zoratti M, Chandy K, Gulbins E, et al. Inhibitors of mitochondrial Kv1.3 channels induce Bax/Bak-independent death of cancer cells. EMBO Mol Med. 2012;4:577-93 pubmed publisher
immunohistochemistry; Xenopus laevis; 1:5000	Harvey W, Xiang M. K+ pump: from caterpillar midgut to human cochlea. J Insect Physiol. 2012;58:590-8 pubmed publisher
western blot; human; 1:1000	Giacomello M, De Mario A, Lopreiato R, Primerano S, Campeol M, Brini M, et al. Mutations in PMCA2 and hereditary deafness: a molecular analysis of the pump defect. Cell Calcium. 2011;50:569-76 pubmed publisher
western blot; mouse	Digel M, Staffer S, Ehehalt F, Stremmel W, Ehehalt R, Fullekrug J. FATP4 contributes as an enzyme to the basal and insulin-mediated fatty acid uptake of C?C?? muscle cells. Am J Physiol Endocrinol Metab. 2011;301:E785-96 pubmed publisher
immunohistochemistry; mouse; 1:500	Billig G, Pál B, Fidzinski P, Jentsch T. Ca2+-activated Cl? currents are dispensable for olfaction. Nat Neurosci. 2011;14:763-9 pubmed publisher
western blot; mouse	Linde C, Baryshnikov S, Mazzocco Spezzia A, Golovina V. Dysregulation of Ca2+ signaling in astrocytes from mice lacking amyloid precursor protein. Am J Physiol Cell Physiol. 2011;300:C1502-12 pubmed publisher
western blot; bovine	Lock J, Sinkins W, Schilling W. Effect of protein S-glutathionylation on Ca2+ homeostasis in cultured aortic endothelial cells. Am J Physiol Heart Circ Physiol. 2011;300:H493-506 pubmed publisher
immunohistochemistry; domestic rabbit; 1:5000 western blot; domestic rabbit; 1:10000	Talarico E. Plasma membrane calcium-ATPase isoform four distribution changes during corneal epithelial wound healing. Mol Vis. 2010;16:2259-72 pubmed
western blot; pigs	Kuszczak I, Samson S, Pande J, Shen D, Grover A. Sodium-calcium exchanger and lipid rafts in pig coronary artery smooth muscle. Biochim Biophys Acta. 2011;1808:589-96 pubmed publisher
western blot; domestic rabbit; 1:1000	Szewczyk M, Pande J, Akolkar G, Grover A. Caloxin 1b3: a novel plasma membrane Ca(2+)-pump isoform 1 selective inhibitor that increases cytosolic Ca(2+) in endothelial cells. Cell Calcium. 2010;48:352-7 pubmed publisher
western blot; human; 1:2000	Grice D, Vetter I, Faddy H, Kenny P, Roberts Thomson S, Monteith G. Golgi calcium pump secretory pathway calcium ATPase 1 (SPCA1) is a key regulator of insulin-like growth factor receptor (IGF1R) processing in the basal-like breast cancer cell line MDA-MB-231. J Biol Chem. 2010;285:37458-66 pubmed publisher
western blot; rat; 1:1000	Zulian A, Baryshnikov S, Linde C, Hamlyn J, Ferrari P, Golovina V. Upregulation of Na+/Ca2+ exchanger and TRPC6 contributes to abnormal Ca2+ homeostasis in arterial smooth muscle cells from Milan hypertensive rats. Am J Physiol Heart Circ Physiol. 2010;299:H624-33 pubmed publisher
western blot; mouse	Pritchard T, Bowman P, Jefferson A, Tosun M, Lynch R, Paul R. Na(+)-K(+)-ATPase and Ca(2+) clearance proteins in smooth muscle: a functional unit. Am J Physiol Heart Circ Physiol. 2010;299:H548-56 pubmed publisher
western blot; human	Sassi N, De Marchi U, Fioretti B, Biasutto L, Gulbins E, Franciolini F, et al. An investigation of the occurrence and properties of the mitochondrial intermediate-conductance Ca2+-activated K+ channel mtKCa3.1. Biochim Biophys Acta. 2010;1797:1260-7 pubmed publisher
western blot; mouse	Louch W, Hougen K, Mørk H, Swift F, Aronsen J, Sjaastad I, et al. Sodium accumulation promotes diastolic dysfunction in end-stage heart failure following Serca2 knockout. J Physiol. 2010;588:465-78 pubmed publisher
western blot; mouse immunocytochemistry; human	Sinkins W, Estacion M, Prasad V, Goel M, Shull G, Kunze D, et al. Maitotoxin converts the plasmalemmal Ca(2+) pump into a Ca(2+)-permeable nonselective cation channel. Am J Physiol Cell Physiol. 2009;297:C1533-43 pubmed publisher
western blot; rat; 1:400	Zaidi A, Fernandes D, Bean J, Michaelis M. Effects of paraquat-induced oxidative stress on the neuronal plasma membrane Ca(2+)-ATPase. Free Radic Biol Med. 2009;47:1507-14 pubmed publisher
western blot; human	De Marchi U, Sassi N, Fioretti B, Catacuzzeno L, Cereghetti G, Szabo I, et al. Intermediate conductance Ca2+-activated potassium channel (KCa3.1) in the inner mitochondrial membrane of human colon cancer cells. Cell Calcium. 2009;45:509-16 pubmed publisher
immunocytochemistry; Xenopus laevis western blot; Xenopus laevis	El Jouni W, Haun S, Machaca K. Internalization of plasma membrane Ca2+-ATPase during Xenopus oocyte maturation. Dev Biol. 2008;324:99-107 pubmed publisher
western blot; rat	O CONNELL K, Gannon J, Doran P, Ohlendieck K. Reduced expression of sarcalumenin and related Ca2+ -regulatory proteins in aged rat skeletal muscle. Exp Gerontol. 2008;43:958-61 pubmed publisher
immunohistochemistry; pigs ; 1:500	Sepulveda M, Berrocal M, Marcos D, Wuytack F, Mata A. Functional and immunocytochemical evidence for the expression and localization of the secretory pathway Ca2+-ATPase isoform 1 (SPCA1) in cerebellum relative to other Ca2+ pumps. J Neurochem. 2007;103:1009-18 pubmed
western blot; human; 1:100	Brown C, Dean W. Regulation of plasma membrane Ca2+-ATPase in human platelets by calpain. Platelets. 2007;18:207-11 pubmed
western blot; bovine; 1:1500	Mas Oliva J, Delgado Coello B. Protein stability and the evolution of the cell membrane. Comp Biochem Physiol C Toxicol Pharmacol. 2007;146:207-13 pubmed
immunocytochemistry; human	Weeraratne S, Valentine M, Cusick M, Delay R, Van Houten J. Plasma membrane calcium pumps in mouse olfactory sensory neurons. Chem Senses. 2006;31:725-30 pubmed
western blot; human	Chaabane C, Corvazier E, Bredoux R, Dally S, Raies A, Villemain A, et al. Sarco/endoplasmic reticulum Ca2+ATPase type 3 isoforms (SERCA3b and SERCA3f): distinct roles in cell adhesion and ER stress. Biochem Biophys Res Commun. 2006;345:1377-85 pubmed
western blot; human	Nurden P, Debili N, Vainchenker W, Bobe R, Bredoux R, Corvazier E, et al. Impaired megakaryocytopoiesis in type 2B von Willebrand disease with severe thrombocytopenia. Blood. 2006;108:2587-95 pubmed
western blot; mouse	Liu L, Ishida Y, Okunade G, Shull G, Paul R. Role of plasma membrane Ca2+-ATPase in contraction-relaxation processes of the bladder: evidence from PMCA gene-ablated mice. Am J Physiol Cell Physiol. 2006;290:C1239-47 pubmed
immunoprecipitation; human	Rimessi A, Coletto L, Pinton P, Rizzuto R, Brini M, Carafoli E. Inhibitory interaction of the 14-3-3{epsilon} protein with isoform 4 of the plasma membrane Ca(2+)-ATPase pump. J Biol Chem. 2005;280:37195-203 pubmed
immunohistochemistry; chicken; 1:500 western blot; chicken; 1:3000	Sepulveda M, Hidalgo Sanchez M, Mata A. A developmental profile of the levels of calcium pumps in chick cerebellum. J Neurochem. 2005;95:673-83 pubmed
western blot; Pacific electric ray	Valverde R, Tortelote G, Lemos T, Mintz E, Vieyra A. Ca2+/calmodulin-dependent protein kinase II is an essential mediator in the coordinated regulation of electrocyte Ca2+-ATPase by calmodulin and protein kinase A. J Biol Chem. 2005;280:30611-8 pubmed
western blot; mouse; 1:1000	Hurtado C, Ander B, Maddaford T, Lukas A, Hryshko L, Pierce G. Adenovirally delivered shRNA strongly inhibits Na+-Ca2+ exchanger expression but does not prevent contraction of neonatal cardiomyocytes. J Mol Cell Cardiol. 2005;38:647-54 pubmed
	Sritangos P, Pena Alarcon E, James A, Sultan A, Richardson D, Bruce J. Plasma Membrane Ca2+ ATPase Isoform 4 (PMCA4) Has an Important Role in Numerous Hallmarks of Pancreatic Cancer. Cancers (Basel). 2020;12: pubmed publisher
	Roest G, Hesemans E, Welkenhuyzen K, Luyten T, Engedal N, Bultynck G, et al. The ER Stress Inducer l-Azetidine-2-Carboxylic Acid Elevates the Levels of Phospho-eIF2α and of LC3-II in a Ca2+-Dependent Manner. Cells. 2018;7: pubmed publisher
	Olgar Y, Durak A, Tuncay E, Bitirim C, Ozcinar E, Inan M, et al. Increased free Zn2+ correlates induction of sarco(endo)plasmic reticulum stress via altered expression levels of Zn2+ -transporters in heart failure. J Cell Mol Med. 2018;22:1944-1956 pubmed publisher
	Schmidt N, Kollewe A, Constantin C, Henrich S, Ritzau Jost A, Bildl W, et al. Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca2+-ATPases and Key Regulators of Ca2+ Clearance. Neuron. 2017;96:827-838.e9 pubmed publisher
	Lisek M, Ferenc B, Studzian M, Pulaski L, Guo F, Zylinska L, et al. Glutamate Deregulation in Ketamine-Induced Psychosis-A Potential Role of PSD95, NMDA Receptor and PMCA Interaction. Front Cell Neurosci. 2017;11:181 pubmed publisher
	Groten C, Rebane J, Hodgson H, Chauhan A, Blohm G, Magoski N. Ca2+ removal by the plasma membrane Ca2+-ATPase influences the contribution of mitochondria to activity-dependent Ca2+ dynamics in Aplysia neuroendocrine cells. J Neurophysiol. 2016;115:2615-34 pubmed publisher
	Berrocal M, Corbacho I, VÃ¡zquez HernÃ¡ndez M, Ãvila J, SepÃºlveda M, Mata A. Inhibition of PMCA activity by tau as a function of aging and Alzheimer's neuropathology. Biochim Biophys Acta. 2015;1852:1465-76 pubmed publisher
	Kruger W, Yun C, Monteith G, Poronnik P. Muscarinic-induced recruitment of plasma membrane Ca2+-ATPase involves PSD-95/Dlg/Zo-1-mediated interactions. J Biol Chem. 2009;284:1820-30 pubmed publisher
	Johnson J, Perkins G, Giddabasappa A, Chaney S, Xiao W, White A, et al. Spatiotemporal regulation of ATP and Ca2+ dynamics in vertebrate rod and cone ribbon synapses. Mol Vis. 2007;13:887-919 pubmed
	Hill J, Williams D, LeMasurier M, Dumont R, Strehler E, Gillespie P. Splice-site A choice targets plasma-membrane Ca2+-ATPase isoform 2 to hair bundles. J Neurosci. 2006;26:6172-80 pubmed
	Li X, Kiedrowski L, Tremblay F, Fernandez F, Perizzolo M, Winkfein R, et al. Importance of K+-dependent Na+/Ca2+-exchanger 2, NCKX2, in motor learning and memory. J Biol Chem. 2006;281:6273-82 pubmed
	Lee W, Robinson J, Holman N, McCall M, Roberts Thomson S, Monteith G. Antisense-mediated Inhibition of the plasma membrane calcium-ATPase suppresses proliferation of MCF-7 cells. J Biol Chem. 2005;280:27076-84 pubmed
	Talarico E, Kennedy B, Marfurt C, Loeffler K, Mangini N. Expression and immunolocalization of plasma membrane calcium ATPase isoforms in human corneal epithelium. Mol Vis. 2005;11:169-78 pubmed
	Burlando B, Bonomo M, Caprì F, Mancinelli G, Pons G, Viarengo A. Different effects of Hg2+ and Cu2+ on mussel (Mytilus galloprovincialis) plasma membrane Ca2+-ATPase: Hg2+ induction of protein expression. Comp Biochem Physiol C Toxicol Pharmacol. 2004;139:201-7 pubmed
	Luo X, Shin D, Wang X, Konieczny S, Muallem S. Aberrant localization of intracellular organelles, Ca2+ signaling, and exocytosis in Mist1 null mice. J Biol Chem. 2005;280:12668-75 pubmed
	Malmberg E, Andersson C, Gentzsch M, Chen J, Mengos A, Cui L, et al. Bcr (breakpoint cluster region) protein binds to PDZ-domains of scaffold protein PDZK1 and vesicle coat protein Mint3. J Cell Sci. 2004;117:5535-41 pubmed
	Rosado J, Saavedra F, Redondo P, Hernández Cruz J, Salido G, Pariente J. Reduced plasma membrane Ca2+-ATPase function in platelets from patients with non-insulin-dependent diabetes mellitus. Haematologica. 2004;89:1142-4 pubmed
	Molina A, Verzi M, Birnbaum A, Yamoah E, Hammar K, Smith P, et al. Neurotransmitter modulation of extracellular H+ fluxes from isolated retinal horizontal cells of the skate. J Physiol. 2004;560:639-57 pubmed
	Krizaj D, Liu X, Copenhagen D. Expression of calcium transporters in the retina of the tiger salamander (Ambystoma tigrinum). J Comp Neurol. 2004;475:463-80 pubmed
	Okunade G, Miller M, Pyne G, Sutliff R, O Connor K, Neumann J, et al. Targeted ablation of plasma membrane Ca2+-ATPase (PMCA) 1 and 4 indicates a major housekeeping function for PMCA1 and a critical role in hyperactivated sperm motility and male fertility for PMCA4. J Biol Chem. 2004;279:33742-50 pubmed
	Rivas F, O Keefe J, Alegre M, Gajewski T. Actin cytoskeleton regulates calcium dynamics and NFAT nuclear duration. Mol Cell Biol. 2004;24:1628-39 pubmed
	Peluso J. Basic fibroblast growth factor (bFGF) regulation of the plasma membrane calcium ATPase (PMCA) as part of an anti-apoptotic mechanism of action. Biochem Pharmacol. 2003;66:1363-9 pubmed
	Shin D, Dehoff M, Luo X, Kang S, Tu J, Nayak S, et al. Homer 2 tunes G protein-coupled receptors stimulus intensity by regulating RGS proteins and PLCbeta GAP activities. J Cell Biol. 2003;162:293-303 pubmed
	Cook L, Zhu C, Hinkle P. Thyrotropin-releasing hormone receptor processing: role of ubiquitination and proteasomal degradation. Mol Endocrinol. 2003;17:1777-91 pubmed
	Reuter H, Henderson S, Han T, Mottino G, Frank J, Ross R, et al. Cardiac excitation-contraction coupling in the absence of Na(+) - Ca2+ exchange. Cell Calcium. 2003;34:19-26 pubmed
	Champion E, Glazier J, Greenwood S, Mann S, Rawlings J, Sibley C, et al. Localization of alkaline phosphatase and Ca2+-ATPase in the cat placenta. Placenta. 2003;24:453-61 pubmed
	Bruce J, Yule D, Shuttleworth T. Ca2+-dependent protein kinase--a modulation of the plasma membrane Ca2+-ATPase in parotid acinar cells. J Biol Chem. 2002;277:48172-81 pubmed
	Magyar C, White K, Rojas R, Apodaca G, Friedman P. Plasma membrane Ca2+-ATPase and NCX1 Na+/Ca2+ exchanger expression in distal convoluted tubule cells. Am J Physiol Renal Physiol. 2002;283:F29-40 pubmed
	Martin V, Bredoux R, Corvazier E, Van Gorp R, Kovacs T, Gelebart P, et al. Three novel sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) 3 isoforms. Expression, regulation, and function of the membranes of the SERCA3 family. J Biol Chem. 2002;277:24442-52 pubmed
	Yarbrough T, Lu T, Lee H, Shibata E. Localization of cardiac sodium channels in caveolin-rich membrane domains: regulation of sodium current amplitude. Circ Res. 2002;90:443-9 pubmed
	Schuh K, Uldrijan S, Telkamp M, Rothlein N, Neyses L. The plasmamembrane calmodulin-dependent calcium pump: a major regulator of nitric oxide synthase I. J Cell Biol. 2001;155:201-5 pubmed
	Daniel E, Jury J, Wang Y. nNOS in canine lower esophageal sphincter: colocalized with Cav-1 and Ca2+-handling proteins?. Am J Physiol Gastrointest Liver Physiol. 2001;281:G1101-14 pubmed
	Dumont R, Lins U, Filoteo A, Penniston J, Kachar B, Gillespie P. Plasma membrane Ca2+-ATPase isoform 2a is the PMCA of hair bundles. J Neurosci. 2001;21:5066-78 pubmed
	Zabe M, Dean W. Plasma membrane Ca(2+)-ATPase associates with the cytoskeleton in activated platelets through a PDZ-binding domain. J Biol Chem. 2001;276:14704-9 pubmed
	Antoine S, Pinet C, Coulombe A. Are B-type Ca2+ channels of cardiac myocytes akin to the passive ion channel in the plasma membrane Ca2+ pump?. J Membr Biol. 2001;179:37-50 pubmed
	Darby P, Kwan C, Daniel E. Caveolae from canine airway smooth muscle contain the necessary components for a role in Ca(2+) handling. Am J Physiol Lung Cell Mol Physiol. 2000;279:L1226-35 pubmed
	Gentzsch M, Riordan J. Localization of sequences within the C-terminal domain of the cystic fibrosis transmembrane conductance regulator which impact maturation and stability. J Biol Chem. 2001;276:1291-8 pubmed
	Sheikh Hamad D, Youker K, Truong L, Nielsen S, Entman M. Osmotically relevant membrane signaling complex: association between HB-EGF, beta(1)-integrin, and CD9 in mTAL. Am J Physiol Cell Physiol. 2000;279:C136-46 pubmed
	Rosado J, Sage S. Regulation of plasma membrane Ca2+-ATPase by small GTPases and phosphoinositides in human platelets. J Biol Chem. 2000;275:19529-35 pubmed
	Blankenship K, Dawson C, Aronoff G, Dean W. Tyrosine phosphorylation of human platelet plasma membrane Ca(2+)-ATPase in hypertension. Hypertension. 2000;35:103-7 pubmed
	Martin V, Bredoux R, Corvazier E, Papp B, Enouf J. Platelet Ca(2+)ATPases : a plural, species-specific, and multiple hypertension-regulated expression system. Hypertension. 2000;35:91-102 pubmed
	Hammes A, Oberdorf Maass S, Rother T, Nething K, Gollnick F, Linz K, et al. Overexpression of the sarcolemmal calcium pump in the myocardium of transgenic rats. Circ Res. 1998;83:877-88 pubmed
	Yamoah E, Lumpkin E, Dumont R, Smith P, Hudspeth A, Gillespie P. Plasma membrane Ca2+-ATPase extrudes Ca2+ from hair cell stereocilia. J Neurosci. 1998;18:610-24 pubmed
	Filoteo A, Elwess N, Enyedi A, Caride A, Aung H, Penniston J. Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies. J Biol Chem. 1997;272:23741-7 pubmed
	Lee M, Xu X, Zeng W, Diaz J, Kuo T, Wuytack F, et al. Polarized expression of Ca2+ pumps in pancreatic and salivary gland cells. Role in initiation and propagation of [Ca2+]i waves. J Biol Chem. 1997;272:15771-6 pubmed
	Dean W, Chen D, Brandt P, Vanaman T. Regulation of platelet plasma membrane Ca2+-ATPase by cAMP-dependent and tyrosine phosphorylation. J Biol Chem. 1997;272:15113-9 pubmed
	White K, Gesek F, Nesbitt T, Drezner M, Friedman P. Molecular dissection of Ca2+ efflux in immortalized proximal tubule cells. J Gen Physiol. 1997;109:217-28 pubmed
	Hammes A, Oberdorf Maass S, Jenatschke S, Pelzer T, Maass A, Gollnick F, et al. Expression of the plasma membrane Ca2+-ATPase in myogenic cells. J Biol Chem. 1996;271:30816-22 pubmed
	Caride A, Filoteo A, Enyedi A, Verma A, Penniston J. Detection of isoform 4 of the plasma membrane calcium pump in human tissues by using isoform-specific monoclonal antibodies. Biochem J. 1996;316 ( Pt 1):353-9 pubmed
	Liu B, Xu X, Fridman R, Muallem S, Kuo T. Consequences of functional expression of the plasma membrane Ca2+ pump isoform 1a. J Biol Chem. 1996;271:5536-44 pubmed
	Schnitzer J, Oh P, Jacobson B, Dvorak A. Caveolae from luminal plasmalemma of rat lung endothelium: microdomains enriched in caveolin, Ca(2+)-ATPase, and inositol trisphosphate receptor. Proc Natl Acad Sci U S A. 1995;92:1759-63 pubmed
	Guerini D, Schroder S, Foletti D, Carafoli E. Isolation and characterization of a stable Chinese hamster ovary cell line overexpressing the plasma membrane Ca(2+)-ATPase. J Biol Chem. 1995;270:14643-50 pubmed
	Poulsen J, Caspersen C, Mathiasen D, East J, Tunwell R, Lai F, et al. Thapsigargin-sensitive Ca(2+)-ATPases account for Ca2+ uptake to inositol 1,4,5-trisphosphate-sensitive and caffeine-sensitive Ca2+ stores in adrenal chromaffin cells. Biochem J. 1995;307 ( Pt 3):749-58 pubmed
	Bokkala S, El Daher S, Kakkar V, Wuytack F, Authi K. Localization and identification of Ca2+ATPases in highly purified human platelet plasma and intracellular membranes. Evidence that the monoclonal antibody PL/IM 430 recognizes the SERCA 3 Ca2+ATPase in human platelets. Biochem J. 1995;306 ( Pt 3):837-42 pubmed
	Dean W, Quinton T. Distribution of plasma membrane Ca(2+)-ATPase and inositol 1,4,5-trisphosphate receptor in human platelet membranes. Cell Calcium. 1995;17:65-70 pubmed
	Borke J, Caride A, Verma A, Penniston J, Kumar R. Plasma membrane calcium pump and 28-kDa calcium binding protein in cells of rat kidney distal tubules. Am J Physiol. 1989;257:F842-9 pubmed
	Borke J, Caride A, Yaksh T, Penniston J, Kumar R. Cerebrospinal fluid calcium homeostasis: evidence for a plasma membrane Ca2+-pump in mammalian choroid plexus. Brain Res. 1989;489:355-60 pubmed
	Kessler F, Bennardini F, Bachs O, Serratosa J, James P, Caride A, et al. Partial purification and characterization of the Ca2(+)-pumping ATPase of the liver plasma membrane. J Biol Chem. 1990;265:16012-9 pubmed
	Kuo T, Wang K, Carlock L, Diglio C, Tsang W. Phorbol ester induces both gene expression and phosphorylation of the plasma membrane Ca2+ pump. J Biol Chem. 1991;266:2520-5 pubmed
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	Wasserman R, Smith C, Smith C, Brindak M, Fullmer C, Krook L, et al. Immunohistochemical localization of a calcium pump and calbindin-D28k in the oviduct of the laying hen. Histochemistry. 1991;96:413-8 pubmed
	Adamo H, Caride A, Penniston J. Use of expression mutants and monoclonal antibodies to map the erythrocyte Ca2+ pump. J Biol Chem. 1992;267:14244-9 pubmed

product information

Product Type :

Antibody

Product Name :

PMCA ATPase Monoclonal Antibody (5F10)

Catalog # :

MA3-914

Quantity :

100 µL

Price :

US 399.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Amphibian, Canine, Chicken, Feline, Human, Mouse, Non-human primate, Ovine, Rabbit, Rat

Applications :

Flow Cytometry: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Frozen): 1:500, Immunohistochemistry (Paraffin): 1:500, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000-1:5,000

Species :

Amphibian, Canine, Chicken, Feline, Human, Mouse, Non-human primate, Ovine, Rabbit, Rat

Clone :

5F10

Isotype :

IgG2a

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The calcium pump of the plasma membrane (PMCA ATPase) pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the sarcoplasmic and endoplasmic reticulum calcium (SERCA) ATPase and the sodium/potassium pump. Studies indicate that there are four known isoforms of the PMCA ATPase which are encoded by a multigene family.

Immunogen :

Purified human erythrocyte calcium ATPase.

Format :

Liquid

Applications w/Dilutions :

Aliases :

2810442I22Rik; 6430519O13Rik; ATP2B; ATP2B1; ATP2B2; ATP2B3; ATP2B4; ATPase isoform 2 Na+K+ transporting beta polypeptide 2; ATPase isoform 2, Na+K+ transporting, beta polypeptide 2; ATPase plasma membrane Ca2+ transporting 1; ATPase plasma membrane Ca2+ transporting 2; ATPase plasma membrane Ca2+ transporting 3; ATPase plasma membrane Ca2+ transporting 4; ATPase, Ca++ transporting, plasma membrane 1; ATPase, Ca++ transporting, plasma membrane 2; ATPase, Ca++ transporting, plasma membrane 3; ATPase, Ca++ transporting, plasma membrane 4; ATP-dependent plasma membrane calcium transporter; Ca2+/Mg2+ ATPase; CFAP39; cilia and flagella associated protein 39; CLA2; D6Abb2e; deaf waddler; dfw; E130111D10Rik; Gena300; jog; Matrix-remodeling-associated protein 1; MXRA1; OPCA; plasma membrane Ca(2+)-ATPase; plasma membrane Ca++-ATPase; plasma membrane Ca2+ pump 2; plasma membrane Ca2+-ATPases 4; plasma membrane calcium ATPase; Plasma membrane calcium ATPase isoform 1; plasma membrane calcium ATPase isoform 1b; Plasma membrane calcium ATPase isoform 2; Plasma membrane calcium ATPase isoform 3; Plasma membrane calcium ATPase isoform 4; plasma membrane calcium ATPase isoform 4a; plasma membrane calcium ATPase isoform 4b; plasma membrane calcium pump; Plasma membrane calcium pump isoform 1; plasma membrane calcium pump isoform 1b; Plasma membrane calcium pump isoform 2; Plasma membrane calcium pump isoform 3; Plasma membrane calcium pump isoform 4; Plasma membrane calcium-transporting ATPase 1; plasma membrane calcium-transporting ATPase 2; Plasma membrane calcium-transporting ATPase 3; plasma membrane calcium-transporting ATPase 4; plasma membrane calcium-transporting ATPase 4; plasma membrane calcium-transporting ATPase 1; PMCA; PMCA1; Pmca1a; Pmca1b; Pmca1c; PMCA1kb; PMCA2; PMCA2a; PMCA2i; Pmca3; PMCA3a; Pmca4; PMCA4a; PMCA4b; PMCA4x; sarcolemmal calcium pump; SCAX1; Tmy; wms; wri; wriggle

more info or order :

Invitrogen product webpage