Calsequestrin Monoclonal Antibody (VIIID12) | Invitrogen MA3-913 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Calsequestrin Monoclonal Antibody (VIIID12)

catalog :

MA3-913

quantity :

100 µL

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

VIIID12

reactivity :

human, mouse, rat, dogs, pigs , domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Invitrogen product webpage

citations: 65

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; 1:200; loading ...; fig 5ab	Tammineni E, Kraeva N, Figueroa L, Manno C, Ibarra C, Klip A, et al. Intracellular calcium leak lowers glucose storage in human muscle, promoting hyperglycemia and diabetes. elife. 2020;9: pubmed publisher
western blot; mouse; loading ...; fig 5a	Eshima H, Tamura Y, Kakehi S, Kurebayashi N, Murayama T, Nakamura K, et al. Long-term, but not short-term high-fat diet induces fiber composition changes and impaired contractile force in mouse fast-twitch skeletal muscle. Physiol Rep. 2017;5: pubmed publisher
western blot; mouse; loading ...; fig 2f	Fajardo V, Gamu D, Mitchell A, Bloemberg D, Bombardier E, Chambers P, et al. Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice. PLoS ONE. 2017;12:e0173708 pubmed publisher
western blot; mouse; 1:2000; fig 3	Bal N, Maurya S, Singh S, Wehrens X, Periasamy M. Increased Reliance on Muscle-based Thermogenesis upon Acute Minimization of Brown Adipose Tissue Function. J Biol Chem. 2016;291:17247-57 pubmed publisher
western blot; rat; 1:3000; fig 3	de Andrade P, Neff L, Strosova M, Arsenijevic D, Patthey Vuadens O, Scapozza L, et al. Caloric restriction induces energy-sparing alterations in skeletal muscle contraction, fiber composition and local thyroid hormone metabolism that persist during catch-up fat upon refeeding. Front Physiol. 2015;6:254 pubmed publisher
immunohistochemistry; mouse; 1:300	Rossi D, Vezzani B, Galli L, Paolini C, Toniolo L, Pierantozzi E, et al. A mutation in the CASQ1 gene causes a vacuolar myopathy with accumulation of sarcoplasmic reticulum protein aggregates. Hum Mutat. 2014;35:1163-70 pubmed publisher
western blot; mouse	Carberry S, Zweyer M, Swandulla D, Ohlendieck K. Application of fluorescence two-dimensional difference in-gel electrophoresis as a proteomic biomarker discovery tool in muscular dystrophy research. Biology (Basel). 2013;2:1438-64 pubmed publisher
western blot; mouse	Rossi D, Bencini C, Maritati M, Benini F, Lorenzini S, Pierantozzi E, et al. Distinct regions of triadin are required for targeting and retention at the junctional domain of the sarcoplasmic reticulum. Biochem J. 2014;458:407-17 pubmed publisher
western blot; rat	Vitadello M, Gherardini J, Gorza L. The stress protein/chaperone Grp94 counteracts muscle disuse atrophy by stabilizing subsarcolemmal neuronal nitric oxide synthase. Antioxid Redox Signal. 2014;20:2479-96 pubmed publisher
western blot; mouse; 1:1000	Manno C, Figueroa L, Royer L, Pouvreau S, Lee C, Volpe P, et al. Altered Ca2+ concentration, permeability and buffering in the myofibre Ca2+ store of a mouse model of malignant hyperthermia. J Physiol. 2013;591:4439-57 pubmed publisher
western blot; mouse	Amoasii L, Hnia K, Chicanne G, Brech A, Cowling B, Müller M, et al. Myotubularin and PtdIns3P remodel the sarcoplasmic reticulum in muscle in vivo. J Cell Sci. 2013;126:1806-19 pubmed publisher
western blot; mouse; 1:3000	Dorchies O, Reutenauer Patte J, Dahmane E, Ismail H, Petermann O, Patthey Vuadens O, et al. The anticancer drug tamoxifen counteracts the pathology in a mouse model of duchenne muscular dystrophy. Am J Pathol. 2013;182:485-504 pubmed
western blot; human	Middlekauff H, Vigna C, Verity M, Fonarow G, Horwich T, Hamilton M, et al. Abnormalities of calcium handling proteins in skeletal muscle mirror those of the heart in humans with heart failure: a shared mechanism?. J Card Fail. 2012;18:724-33 pubmed publisher
western blot; mouse	Boncompagni S, Thomas M, Lopez J, Allen P, Yuan Q, Kranias E, et al. Triadin/Junctin double null mouse reveals a differential role for Triadin and Junctin in anchoring CASQ to the jSR and regulating Ca(2+) homeostasis. PLoS ONE. 2012;7:e39962 pubmed publisher
western blot; mouse; 1:500	DiFranco M, Tran P, Quiñonez M, Vergara J. Functional expression of transgenic 1sDHPR channels in adult mammalian skeletal muscle fibres. J Physiol. 2011;589:1421-42 pubmed publisher
western blot; mouse	Eltit J, Feng W, Lopez J, Padilla I, Pessah I, Molinski T, et al. Ablation of skeletal muscle triadin impairs FKBP12/RyR1 channel interactions essential for maintaining resting cytoplasmic Ca2+. J Biol Chem. 2010;285:38453-62 pubmed publisher
immunohistochemistry; mouse western blot; mouse	Vassilopoulos S, Oddoux S, Groh S, Cacheux M, Faure J, Brocard J, et al. Caveolin 3 is associated with the calcium release complex and is modified via in vivo triadin modification. Biochemistry. 2010;49:6130-5 pubmed publisher
western blot; mouse	Lewis C, Ohlendieck K. Proteomic profiling of naturally protected extraocular muscles from the dystrophin-deficient mdx mouse. Biochem Biophys Res Commun. 2010;396:1024-9 pubmed publisher
immunocytochemistry; human	Pertille A, de Carvalho C, Matsumura C, Neto H, Marques M. Calcium-binding proteins in skeletal muscles of the mdx mice: potential role in the pathogenesis of Duchenne muscular dystrophy. Int J Exp Pathol. 2010;91:63-71 pubmed publisher
western blot; mouse	Oddoux S, Brocard J, Schweitzer A, Szentesi P, Giannesini B, Brocard J, et al. Triadin deletion induces impaired skeletal muscle function. J Biol Chem. 2009;284:34918-29 pubmed publisher
western blot; mouse	Ottenheijm C, Hidalgo C, Rost K, Gotthardt M, Granzier H. Altered contractility of skeletal muscle in mice deficient in titin's M-band region. J Mol Biol. 2009;393:10-26 pubmed publisher
immunohistochemistry; mouse western blot; mouse	Ferretti R, Marques M, Pertille A, Santo Neto H. Sarcoplasmic-endoplasmic-reticulum Ca2+-ATPase and calsequestrin are overexpressed in spared intrinsic laryngeal muscles of dystrophin-deficient mdx mice. Muscle Nerve. 2009;39:609-15 pubmed publisher
western blot; mouse	Matsumura C, Pertille A, Albuquerque T, Santo Neto H, Marques M. Diltiazem and verapamil protect dystrophin-deficient muscle fibers of MDX mice from degeneration: a potential role in calcium buffering and sarcolemmal stability. Muscle Nerve. 2009;39:167-76 pubmed publisher
western blot; rat	O CONNELL K, Gannon J, Doran P, Ohlendieck K. Reduced expression of sarcalumenin and related Ca2+ -regulatory proteins in aged rat skeletal muscle. Exp Gerontol. 2008;43:958-61 pubmed publisher
western blot; domestic rabbit; 1:2500; fig 8	Phimister A, Lango J, Lee E, Ernst Russell M, Takeshima H, Ma J, et al. Conformation-dependent stability of junctophilin 1 (JP1) and ryanodine receptor type 1 (RyR1) channel complex is mediated by their hyper-reactive thiols. J Biol Chem. 2007;282:8667-77 pubmed
western blot; rat; 1:1,000	Rodriguez P, Mitton B, Waggoner J, Kranias E. Identification of a novel phosphorylation site in protein phosphatase inhibitor-1 as a negative regulator of cardiac function. J Biol Chem. 2006;281:38599-608 pubmed
western blot; pigs	Schulz J, Palmer N, Steckelberg J, Jones S, Zeece M. Microarray profiling of skeletal muscle sarcoplasmic reticulum proteins. Biochim Biophys Acta. 2006;1764:1429-35 pubmed
western blot; mouse	Doran P, Dowling P, Donoghue P, Buffini M, Ohlendieck K. Reduced expression of regucalcin in young and aged mdx diaphragm indicates abnormal cytosolic calcium handling in dystrophin-deficient muscle. Biochim Biophys Acta. 2006;1764:773-85 pubmed
western blot; rat	Mulvey C, Harno E, Keenan A, Ohlendieck K. Expression of the skeletal muscle dystrophin-dystroglycan complex and syntrophin-nitric oxide synthase complex is severely affected in the type 2 diabetic Goto-Kakizaki rat. Eur J Cell Biol. 2005;84:867-83 pubmed
western blot; domestic rabbit	Donoghue P, Doran P, Dowling P, Ohlendieck K. Differential expression of the fast skeletal muscle proteome following chronic low-frequency stimulation. Biochim Biophys Acta. 2005;1752:166-76 pubmed
immunohistochemistry; rat	Nissinen M, Kaisto T, Salmela P, Peltonen J, Metsikkö K. Restricted distribution of mRNAs encoding a sarcoplasmic reticulum or transverse tubule protein in skeletal myofibers. J Histochem Cytochem. 2005;53:217-27 pubmed
	Wyckelsma V, Venckunas T, Houweling P, Schlittler M, Lauschke V, Tiong C, et al. Loss of α-actinin-3 during human evolution provides superior cold resilience and muscle heat generation. Am J Hum Genet. 2021;108:446-457 pubmed publisher
	Wyckelsma V, Venckunas T, Brazaitis M, Gastaldello S, Snieckus A, Eimantas N, et al. Vitamin C and E Treatment Blunts Sprint Interval Training-Induced Changes in Inflammatory Mediator-, Calcium-, and Mitochondria-Related Signaling in Recreationally Active Elderly Humans. Antioxidants (Basel). 2020;9: pubmed publisher
	Maurya S, Herrera J, Sahoo S, Reis F, Vega R, Kelly D, et al. Sarcolipin Signaling Promotes Mitochondrial Biogenesis and Oxidative Metabolism in Skeletal Muscle. Cell Rep. 2018;24:2919-2931 pubmed publisher
	Tinklenberg J, Siebers E, Beatka M, Meng H, Yang L, Zhang Z, et al. Myostatin inhibition using mRK35 produces skeletal muscle growth and tubular aggregate formation in wild type and TgACTA1D286G nemaline myopathy mice. Hum Mol Genet. 2018;27:638-648 pubmed publisher
	Bal N, Singh S, Reis F, Maurya S, Pani S, Rowland L, et al. Both brown adipose tissue and skeletal muscle thermogenesis processes are activated during mild to severe cold adaptation in mice. J Biol Chem. 2017;292:16616-16625 pubmed publisher
	Ravel Chapuis A, Belanger G, Cote J, Michel R, Jasmin B. Misregulation of calcium-handling proteins promotes hyperactivation of calcineurin-NFAT signaling in skeletal muscle of DM1 mice. Hum Mol Genet. 2017;26:2192-2206 pubmed publisher
	Jayasinghe I, Munro M, Baddeley D, Launikonis B, Soeller C. Observation of the molecular organization of calcium release sites in fast- and slow-twitch skeletal muscle with nanoscale imaging. J R Soc Interface. 2014;11: pubmed publisher
	Chen Y, Xue S, Zou J, Lopez J, Yang J, Perez C. Myoplasmic resting Ca2+ regulation by ryanodine receptors is under the control of a novel Ca2+-binding region of the receptor. Biochem J. 2014;460:261-71 pubmed publisher
	Bal N, Maurya S, Sopariwala D, Sahoo S, Gupta S, Shaikh S, et al. Sarcolipin is a newly identified regulator of muscle-based thermogenesis in mammals. Nat Med. 2012;18:1575-9 pubmed publisher
	Al Qusairi L, Weiss N, Toussaint A, Berbey C, Messaddeq N, Kretz C, et al. T-tubule disorganization and defective excitation-contraction coupling in muscle fibers lacking myotubularin lipid phosphatase. Proc Natl Acad Sci U S A. 2009;106:18763-8 pubmed publisher
	Shen X, Franzini Armstrong C, Lopez J, Jones L, Kobayashi Y, Wang Y, et al. Triadins modulate intracellular Ca(2+) homeostasis but are not essential for excitation-contraction coupling in skeletal muscle. J Biol Chem. 2007;282:37864-74 pubmed
	Vassilopoulos S, Thevenon D, Rezgui S, Brocard J, Chapel A, Lacampagne A, et al. Triadins are not triad-specific proteins: two new skeletal muscle triadins possibly involved in the architecture of sarcoplasmic reticulum. J Biol Chem. 2005;280:28601-9 pubmed
	Doran P, Dowling P, Lohan J, McDonnell K, Poetsch S, Ohlendieck K. Subproteomics analysis of Ca+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle. Eur J Biochem. 2004;271:3943-52 pubmed
	Del Monte F, Dalal R, Tabchy A, Couget J, Bloch K, Peterson R, et al. Transcriptional changes following restoration of SERCA2a levels in failing rat hearts. FASEB J. 2004;18:1474-6 pubmed
	Yatani A, Kim S, Kudej R, Wang Q, Depre C, Irie K, et al. Insights into cardioprotection obtained from study of cellular Ca2+ handling in myocardium of true hibernating mammals. Am J Physiol Heart Circ Physiol. 2004;286:H2219-28 pubmed
	Dowling P, Doran P, Ohlendieck K. Drastic reduction of sarcalumenin in Dp427 (dystrophin of 427 kDa)-deficient fibres indicates that abnormal calcium handling plays a key role in muscular dystrophy. Biochem J. 2004;379:479-88 pubmed
	Glover L, Heffron J, Ohlendieck K. Increased sensitivity of the ryanodine receptor to halothane-induced oligomerization in malignant hyperthermia-susceptible human skeletal muscle. J Appl Physiol (1985). 2004;96:11-8 pubmed
	Mulvey C, Ohlendieck K. Use of continuous-elution gel electrophoresis as a preparative tool for blot overlay analysis. Anal Biochem. 2003;319:122-30 pubmed
	Dowling P, Lohan J, Ohlendieck K. Comparative analysis of Dp427-deficient mdx tissues shows that the milder dystrophic phenotype of extraocular and toe muscle fibres is associated with a persistent expression of beta-dystroglycan. Eur J Cell Biol. 2003;82:222-30 pubmed
	O Reilly C, Pette D, Ohlendieck K. Increased expression of the nicotinic acetylcholine receptor in stimulated muscle. Biochem Biophys Res Commun. 2003;300:585-91 pubmed
	Glover L, Quinn S, Ryan M, Pette D, Ohlendieck K. Supramolecular calsequestrin complex. Eur J Biochem. 2002;269:4607-16 pubmed
	Semsarian C, Ahmad I, Giewat M, Georgakopoulos D, Schmitt J, McConnell B, et al. The L-type calcium channel inhibitor diltiazem prevents cardiomyopathy in a mouse model. J Clin Invest. 2002;109:1013-20 pubmed
	Culligan K, Banville N, Dowling P, Ohlendieck K. Drastic reduction of calsequestrin-like proteins and impaired calcium binding in dystrophic mdx muscle. J Appl Physiol (1985). 2002;92:435-45 pubmed
	Antonucci D, Lim S, Vassanelli S, Trimmer J. Dynamic localization and clustering of dendritic Kv2.1 voltage-dependent potassium channels in developing hippocampal neurons. Neuroscience. 2001;108:69-81 pubmed
	Glover L, Culligan K, Cala S, Mulvey C, Ohlendieck K. Calsequestrin binds to monomeric and complexed forms of key calcium-handling proteins in native sarcoplasmic reticulum membranes from rabbit skeletal muscle. Biochim Biophys Acta. 2001;1515:120-32 pubmed
	del Monte F, Williams E, Lebeche D, Schmidt U, Rosenzweig A, Gwathmey J, et al. Improvement in survival and cardiac metabolism after gene transfer of sarcoplasmic reticulum Ca(2+)-ATPase in a rat model of heart failure. Circulation. 2001;104:1424-9 pubmed
	Hunter R, Mitchell Felton H, Essig D, Kandarian S. Expression of endoplasmic reticulum stress proteins during skeletal muscle disuse atrophy. Am J Physiol Cell Physiol. 2001;281:C1285-90 pubmed
	Thompson C, Dorup I, Ahn J, Leong P, McDonough A. Glucocorticoids increase sodium pump alpha(2)- and beta(1)-subunit abundance and mRNA in rat skeletal muscle. Am J Physiol Cell Physiol. 2001;280:C509-16 pubmed
	Harmon S, Froemming G, Leisner E, Pette D, Ohlendieck K. Low-frequency stimulation of fast muscle affects the abundance of Ca(2+)-ATPase but not its oligomeric status. J Appl Physiol (1985). 2001;90:371-9 pubmed
	Cavagna M, O Donnell J, Sumbilla C, Inesi G, Klein M. Exogenous Ca2+-ATPase isoform effects on Ca2+ transients of embryonic chicken and neonatal rat cardiac myocytes. J Physiol. 2000;528 Pt 1:53-63 pubmed
	Moore R, Nguyen H, Galceran J, Pessah I, Allen P. A transgenic myogenic cell line lacking ryanodine receptor protein for homologous expression studies: reconstitution of Ry1R protein and function. J Cell Biol. 1998;140:843-51 pubmed
	Balagopal P, Rooyackers O, Adey D, Ades P, Nair K. Effects of aging on in vivo synthesis of skeletal muscle myosin heavy-chain and sarcoplasmic protein in humans. Am J Physiol. 1997;273:E790-800 pubmed publisher
	Knudson C, Chaudhari N, Sharp A, Powell J, Beam K, Campbell K. Specific absence of the alpha 1 subunit of the dihydropyridine receptor in mice with muscular dysgenesis. J Biol Chem. 1989;264:1345-8 pubmed
	Ohlendieck K, Briggs F, Lee K, Wechsler A, Campbell K. Analysis of excitation-contraction-coupling components in chronically stimulated canine skeletal muscle. Eur J Biochem. 1991;202:739-47 pubmed

image

image 1 :

Immunofluorescent analysis of Calsequestrin using Anti-Calsequestrin Monoclonal Antibody (VIIID12) (Product# MA3-913) shows staining in MCF-7 Cells. Calsequestrin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calsequestrin (Product# MA3-913) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

Calsequestrin Monoclonal Antibody (VIIID12)

Catalog # :

MA3-913

Quantity :

100 µL

Price :

US 399.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Canine, Chicken, Human, Mouse, Porcine, Rabbit, Rat

Applications :

Immunocytochemistry: 1:50-1:500, Immunohistochemistry: 1:20, Western Blot: 1:1,000

Species :

Canine, Chicken, Human, Mouse, Porcine, Rabbit, Rat

Clone :

VIIID12

Isotype :

IgG2b

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The sarcoplasmic reticulum (SR) is, in part, responsible for maintaining the level of intracellular calcium in cardiac and skeletal muscle by storing and releasing calcium. Several intralumenal SR calcium binding proteins have been identified, the most prominent of these is calsequestrin. Calsequstrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium.

Immunogen :

Purified rabbit skeletal muscle sarcoplasmic reticulum.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:50-1:500, Immunohistochemistry: 1:20, Western Blot: 1:1,000

Aliases :

Aspartactin; calmitin; calmitine; calsequestrin; calsequestrin 1; calsequestrin 1 (fast-twitch, skeletal muscle); calsequestrin, skeletal muscle isoform; Calsequestrin-1; calsquestrin 1; CASQ; Casq1; CSQ; CSQ1; CSQ-1; Laminin-binding protein; PDIB1; sCSQ; skeletal muscle calsequestrin 1; VMCQA

more info or order :

Invitrogen product webpage