SERCA1 ATPase Monoclonal Antibody (VE121G9) | Invitrogen MA3-912 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

SERCA1 ATPase Monoclonal Antibody (VE121G9)

catalog :

MA3-912

quantity :

100 µg

price :

US 412.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

VE121G9

reactivity :

human, mouse, rat, dogs, pigs , zebrafish , domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, proximity ligation assay

more info or order :

Invitrogen product webpage

citations: 66

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; fig 3d	Pan S, Zhao N, Feng X, Jie Y, Jin Z. Conversion of mouse embryonic fibroblasts into neural crest cells and functional corneal endothelia by defined small molecules. Sci Adv. 2021;7: pubmed publisher
western blot; mouse; 1:1000; fig 5a	Huang M, Lee K, Kim K, Ahn M, Cho C, Kim D, et al. The maintenance ability and Ca2+ availability of skeletal muscle are enhanced by sildenafil. Exp Mol Med. 2016;48:e278 pubmed publisher
western blot; human; loading ...; fig 4	Skovgaard C, Almquist N, Bangsbo J. Effect of increased and maintained frequency of speed endurance training on performance and muscle adaptations in runners. J Appl Physiol (1985). 2017;122:48-59 pubmed publisher
proximity ligation assay; human; 1:500; fig 5 immunohistochemistry - frozen section; human; 1:500; fig 3 immunoprecipitation; human; fig 5 western blot; human; fig 2 western blot; mouse; fig 1	Toral Ojeda I, Aldanondo G, Lasa Elgarresta J, Lasa Fernández H, Fernandez Torron R, Lopez de Munain A, et al. Calpain 3 deficiency affects SERCA expression and function in the skeletal muscle. Expert Rev Mol Med. 2016;18:e7 pubmed publisher
western blot; zebrafish ; fig 2	Furlan S, Mosole S, Murgia M, Nagaraj N, Argenton F, Volpe P, et al. Calsequestrins in skeletal and cardiac muscle from adult Danio rerio. J Muscle Res Cell Motil. 2016;37:27-39 pubmed publisher
western blot; human; fig 1	Mohr M, Thomassen M, Girard O, Racinais S, Nybo L. Muscle variables of importance for physiological performance in competitive football. Eur J Appl Physiol. 2016;116:251-62 pubmed publisher
immunohistochemistry - frozen section; human; 1:2500; fig 2 western blot; human; 1:2500; fig 1b	Zhao Y, Ogawa H, Yonekura S, Mitsuhashi H, Mitsuhashi S, Nishino I, et al. Functional analysis of SERCA1b, a highly expressed SERCA1 variant in myotonic dystrophy type 1 muscle. Biochim Biophys Acta. 2015;1852:2042-7 pubmed publisher
western blot; human; fig 3g	Hostrup M, Kalsen A, Onslev J, Jessen S, Haase C, Habib S, et al. Mechanisms underlying enhancements in muscle force and power output during maximal cycle ergometer exercise induced by chronic Î²2-adrenergic stimulation in men. J Appl Physiol (1985). 2015;119:475-86 pubmed publisher
western blot; human; 1:10,000; fig 3	Lane Donovan C, Philips G, Wasser C, Durakoglugil M, Masiulis I, Upadhaya A, et al. Reelin protects against amyloid Î² toxicity in vivo. Sci Signal. 2015;8:ra67 pubmed publisher
western blot; human; 1:10,000; fig 3	Christensen P, Gunnarsson T, Thomassen M, Wilkerson D, Nielsen J, Bangsbo J. Unchanged content of oxidative enzymes in fast-twitch muscle fibers and VË™O2 kinetics after intensified training in trained cyclists. Physiol Rep. 2015;3: pubmed publisher
western blot; mouse; 1:1000; fig 1b	TÃ³th A, Fodor J, Vincze J, OlÃ¡h T, JuhÃ¡sz T, ZÃ¡kÃ¡ny R, et al. The Effect of SERCA1b Silencing on the Differentiation and Calcium Homeostasis of C2C12 Skeletal Muscle Cells. PLoS ONE. 2015;10:e0123583 pubmed publisher
immunohistochemistry - frozen section; human; fig 3	Zimmermann C, Kalepu R, Ponfick M, Reichel H, Cakir B, Zierz S, et al. Histological characterization and biochemical analysis of paraspinal muscles in neuromuscularly healthy subjects. Muscle Nerve. 2015;52:45-54 pubmed publisher
western blot; human	Skovgaard C, Christensen P, Larsen S, Andersen T, Thomassen M, Bangsbo J. Concurrent speed endurance and resistance training improves performance, running economy, and muscle NHE1 in moderately trained runners. J Appl Physiol (1985). 2014;117:1097-109 pubmed publisher
western blot; human	Nordsborg N, Siebenmann C, Jacobs R, Rasmussen P, Diaz V, Robach P, et al. Four weeks of normobaric "live high-train low" do not alter muscular or systemic capacity for maintaining pH and K? homeostasis during intense exercise. J Appl Physiol (1985). 2012;112:2027-36 pubmed publisher
western blot; rat	Dremina E, Sharov V, SCHONEICH C. Heat-shock proteins attenuate SERCA inactivation by the anti-apoptotic protein Bcl-2: possible implications for the ER Ca2+-mediated apoptosis. Biochem J. 2012;444:127-39 pubmed publisher
western blot; human; 1:2500	Majerczak J, Korostynski M, Nieckarz Z, Szkutnik Z, Duda K, Zoladz J. Endurance training decreases the non-linearity in the oxygen uptake-power output relationship in humans. Exp Physiol. 2012;97:386-99 pubmed publisher
immunohistochemistry; mouse	Wrede A, Margraf N, Goebel H, Deuschl G, Schulz Schaeffer W. Myofibrillar disorganization characterizes myopathy of camptocormia in Parkinson's disease. Acta Neuropathol. 2012;123:419-32 pubmed publisher
western blot; rat; 1:2500	James R, Walter I, Seebacher F. Variation in expression of calcium-handling proteins is associated with inter-individual differences in mechanical performance of rat (Rattus norvegicus) skeletal muscle. J Exp Biol. 2011;214:3542-8 pubmed publisher
immunohistochemistry; mouse western blot; mouse	Sjåland C, Lunde P, Swift F, Munkvik M, Ericsson M, Lunde M, et al. Slowed relaxation and preserved maximal force in soleus muscles of mice with targeted disruption of the Serca2 gene in skeletal muscle. J Physiol. 2011;589:6139-55 pubmed publisher
western blot; mouse	Staunton L, Jockusch H, Wiegand C, Albrecht T, Ohlendieck K. Identification of secondary effects of hyperexcitability by proteomic profiling of myotonic mouse muscle. Mol Biosyst. 2011;7:2480-9 pubmed publisher
western blot; mouse	Lewis C, Ohlendieck K. Proteomic profiling of naturally protected extraocular muscles from the dystrophin-deficient mdx mouse. Biochem Biophys Res Commun. 2010;396:1024-9 pubmed publisher
immunohistochemistry; dogs; 1:1000	Summerfield N, Peters M, Hercock C, Mobasheri A, Young I. Immunohistochemical evidence for expression of fast-twitch type sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA1) in German shepherd dogs with dilated cardiomyopathy myocardium. J Vet Cardiol. 2010;12:17-23 pubmed publisher
western blot; human	Munkvik M, Rehn T, Slettaløkken G, Hasic A, Hallen J, Sjaastad I, et al. Training effects on skeletal muscle calcium handling in human chronic heart failure. Med Sci Sports Exerc. 2010;42:847-55 pubmed publisher
immunohistochemistry; rat western blot; rat	O CONNELL K, Ohlendieck K. Proteomic DIGE analysis of the mitochondria-enriched fraction from aged rat skeletal muscle. Proteomics. 2009;9:5509-24 pubmed publisher
western blot; mouse	Ottenheijm C, Hidalgo C, Rost K, Gotthardt M, Granzier H. Altered contractility of skeletal muscle in mice deficient in titin's M-band region. J Mol Biol. 2009;393:10-26 pubmed publisher
western blot; domestic rabbit	Mahaney J, Thomas D, Farrance I, Froehlich J. Intermolecular interactions in the mechanism of skeletal muscle sarcoplasmic reticulum Ca(2+)-ATPase (SERCA1): evidence for a triprotomer. Biochemistry. 2008;47:13711-25 pubmed publisher
western blot; human; 1:2,500	Majerczak J, Karasinski J, Zoladz J. Training induced decrease in oxygen cost of cycling is accompanied by down-regulation of SERCA expression in human vastus lateralis muscle. J Physiol Pharmacol. 2008;59:589-602 pubmed
western blot; rat	O CONNELL K, Gannon J, Doran P, Ohlendieck K. Reduced expression of sarcalumenin and related Ca2+ -regulatory proteins in aged rat skeletal muscle. Exp Gerontol. 2008;43:958-61 pubmed publisher
western blot; domestic rabbit	Inesi G, Lewis D, Toyoshima C, Hirata A, de Meis L. Conformational fluctuations of the Ca2+-ATPase in the native membrane environment. Effects of pH, temperature, catalytic substrates, and thapsigargin. J Biol Chem. 2008;283:1189-96 pubmed publisher
immunohistochemistry; rat	Goel M, Zuo C, Sinkins W, Schilling W. TRPC3 channels colocalize with Na+/Ca2+ exchanger and Na+ pump in axial component of transverse-axial tubular system of rat ventricle. Am J Physiol Heart Circ Physiol. 2007;292:H874-83 pubmed
western blot; pigs	Schulz J, Palmer N, Steckelberg J, Jones S, Zeece M. Microarray profiling of skeletal muscle sarcoplasmic reticulum proteins. Biochim Biophys Acta. 2006;1764:1429-35 pubmed
western blot; human	Duhamel T, Green H, Perco J, Ouyang J. Comparative effects of a low-carbohydrate diet and exercise plus a low-carbohydrate diet on muscle sarcoplasmic reticulum responses in males. Am J Physiol Cell Physiol. 2006;291:C607-17 pubmed
western blot; human	Duhamel T, Perco J, Green H. Manipulation of dietary carbohydrates after prolonged effort modifies muscle sarcoplasmic reticulum responses in exercising males. Am J Physiol Regul Integr Comp Physiol. 2006;291:R1100-10 pubmed
immunohistochemistry; mouse; 1:500	Rudolf R, Magalhães P, Pozzan T. Direct in vivo monitoring of sarcoplasmic reticulum Ca2+ and cytosolic cAMP dynamics in mouse skeletal muscle. J Cell Biol. 2006;173:187-93 pubmed
western blot; rat	Dremina E, Sharov V, SCHONEICH C. Displacement of SERCA from SR lipid caveolae-related domains by Bcl-2: a possible mechanism for SERCA inactivation. Biochemistry. 2006;45:175-84 pubmed
western blot; human; 1:3000	Duhamel T, Green H, Perco J, Ouyang J. Metabolic and sarcoplasmic reticulum Ca2+ cycling responses in human muscle 4 days following prolonged exercise. Can J Physiol Pharmacol. 2005;83:643-55 pubmed
immunohistochemistry; human; 1:200 western blot; human; 1:2500	Nguyen T, Rubinstein N, Vijayasarathy C, Rome L, Kaiser L, Shrager J, et al. Effect of chronic obstructive pulmonary disease on calcium pump ATPase expression in human diaphragm. J Appl Physiol (1985). 2005;98:2004-10 pubmed
	Tammineni E, Kraeva N, Figueroa L, Manno C, Ibarra C, Klip A, et al. Intracellular calcium leak lowers glucose storage in human muscle, promoting hyperglycemia and diabetes. elife. 2020;9: pubmed publisher
	Gunnarsson T, Brandt N, Fiorenza M, Hostrup M, Pilegaard H, Bangsbo J. Inclusion of sprints in moderate intensity continuous training leads to muscle oxidative adaptations in trained individuals. Physiol Rep. 2019;7:e13976 pubmed publisher
	Skovgaard C, Christiansen D, Christensen P, Almquist N, Thomassen M, Bangsbo J. Effect of speed endurance training and reduced training volume on running economy and single muscle fiber adaptations in trained runners. Physiol Rep. 2018;6: pubmed publisher
	Huang Y, Shen Z, Wu C, Teng Y, Liao C, Kao C, et al. Comparative proteomic profiling reveals a role for Cisd2 in skeletal muscle aging. Aging Cell. 2017;: pubmed publisher
	Skovgaard C, Almquist N, Kvorning T, Christensen P, Bangsbo J. Effect of tapering after a period of high-volume sprint interval training on running performance and muscular adaptations in moderately trained runners. J Appl Physiol (1985). 2018;124:259-267 pubmed publisher
	Ravel Chapuis A, Belanger G, Cote J, Michel R, Jasmin B. Misregulation of calcium-handling proteins promotes hyperactivation of calcineurin-NFAT signaling in skeletal muscle of DM1 mice. Hum Mol Genet. 2017;26:2192-2206 pubmed publisher
	Nyberg M, Fiorenza M, Lund A, Christensen M, RÃ¸mer T, Piil P, et al. Adaptations to Speed Endurance Training in Highly Trained Soccer Players. Med Sci Sports Exerc. 2016;48:1355-64 pubmed publisher
	Burr A, Millay D, Goonasekera S, Park K, Sargent M, Collins J, et al. Na+ dysregulation coupled with Ca2+ entry through NCX1 promotes muscular dystrophy in mice. Mol Cell Biol. 2014;34:1991-2002 pubmed publisher
	SCHONEICH C, Dremina E, Galeva N, Sharov V. Apoptosis in differentiating C2C12 muscle cells selectively targets Bcl-2-deficient myotubes. Apoptosis. 2014;19:42-57 pubmed publisher
	Thomassen M, Murphy R, Bangsbo J. Fibre type-specific change in FXYD1 phosphorylation during acute intense exercise in humans. J Physiol. 2013;591:1523-33 pubmed publisher
	Zoladz J, Grassi B, Majerczak J, Szkutnik Z, Korostyński M, Karasinski J, et al. Training-induced acceleration of O(2) uptake on-kinetics precedes muscle mitochondrial biogenesis in humans. Exp Physiol. 2013;98:883-98 pubmed publisher
	Tadini Buoninsegni F, Bartolommei G, Moncelli M, Tal D, Lewis D, Inesi G. Effects of high-affinity inhibitors on partial reactions, charge movements, and conformational States of the Ca2+ transport ATPase (sarco-endoplasmic reticulum Ca2+ ATPase). Mol Pharmacol. 2008;73:1134-40 pubmed publisher
	Lunde P, Sejersted O, Thorud H, Tønnessen T, Henriksen U, Christensen G, et al. Effects of congestive heart failure on Ca2+ handling in skeletal muscle during fatigue. Circ Res. 2006;98:1514-9 pubmed
	Radzyukevich T, Moseley A, Shelly D, Redden G, Behbehani M, Lingrel J, et al. The Na(+)-K(+)-ATPase alpha2-subunit isoform modulates contractility in the perinatal mouse diaphragm. Am J Physiol Cell Physiol. 2004;287:C1300-10 pubmed
	Dremina E, Sharov V, Kumar K, Zaidi A, Michaelis E, SCHONEICH C. Anti-apoptotic protein Bcl-2 interacts with and destabilizes the sarcoplasmic/endoplasmic reticulum Ca2+-ATPase (SERCA). Biochem J. 2004;383:361-70 pubmed
	Glover L, Heffron J, Ohlendieck K. Increased sensitivity of the ryanodine receptor to halothane-induced oligomerization in malignant hyperthermia-susceptible human skeletal muscle. J Appl Physiol (1985). 2004;96:11-8 pubmed
	Tupling A, Green H, Roy B, Grant S, Ouyang J. Paradoxical effects of prior activity on human sarcoplasmic reticulum Ca2+-ATPase response to exercise. J Appl Physiol (1985). 2003;95:138-44 pubmed
	Minamisawa S, Wang Y, Chen J, Ishikawa Y, Chien K, Matsuoka R. Atrial chamber-specific expression of sarcolipin is regulated during development and hypertrophic remodeling. J Biol Chem. 2003;278:9570-5 pubmed
	Johansson C, Lunde P, Gothe S, Lannergren J, Westerblad H. Isometric force and endurance in skeletal muscle of mice devoid of all known thyroid hormone receptors. J Physiol. 2003;547:789-96 pubmed
	McGowan T, Madesh M, Zhu Y, Wang L, Russo M, Deelman L, et al. TGF-beta-induced Ca(2+) influx involves the type III IP(3) receptor and regulates actin cytoskeleton. Am J Physiol Renal Physiol. 2002;282:F910-20 pubmed
	Daiho T, Yamasaki K, Saino T, Kamidochi M, Satoh K, Iizuka H, et al. Mutations of either or both Cys876 and Cys888 residues of sarcoplasmic reticulum Ca2+-ATPase result in a complete loss of Ca2+ transport activity without a loss of Ca2+-dependent ATPase activity. Role of the CYS876-CYS888 disulfide bond. J Biol Chem. 2001;276:32771-8 pubmed
	Lunde P, Dahlstedt A, Bruton J, Lannergren J, Thoren P, Sejersted O, et al. Contraction and intracellular Ca(2+) handling in isolated skeletal muscle of rats with congestive heart failure. Circ Res. 2001;88:1299-305 pubmed
	Dode L, Van Baelen K, Wuytack F, Dean W. Low temperature molecular adaptation of the skeletal muscle sarco(endo)plasmic reticulum Ca2+-ATPase 1 (SERCA 1) in the wood frog (Rana sylvatica). J Biol Chem. 2001;276:3911-9 pubmed
	Ortenblad N, Lunde P, Levin K, Andersen J, Pedersen P. Enhanced sarcoplasmic reticulum Ca(2+) release following intermittent sprint training. Am J Physiol Regul Integr Comp Physiol. 2000;279:R152-60 pubmed
	Johansson C, Lannergren J, Lunde P, Vennstrom B, Thoren P, Westerblad H. Isometric force and endurance in soleus muscle of thyroid hormone receptor-alpha(1)- or -beta-deficient mice. Am J Physiol Regul Integr Comp Physiol. 2000;278:R598-603 pubmed
	Daiho T, Yamasaki K, Suzuki H, Saino T, Kanazawa T. Deletions or specific substitutions of a few residues in the NH(2)-terminal region (Ala(3) to Thr(9)) of sarcoplasmic reticulum Ca(2+)-ATPase cause inactivation and rapid degradation of the enzyme expressed in COS-1 cells. J Biol Chem. 1999;274:23910-5 pubmed
	Knudson C, Chaudhari N, Sharp A, Powell J, Beam K, Campbell K. Specific absence of the alpha 1 subunit of the dihydropyridine receptor in mice with muscular dysgenesis. J Biol Chem. 1989;264:1345-8 pubmed
	Molnar E, Seidler N, Jona I, Martonosi A. The binding of monoclonal and polyclonal antibodies to the Ca2(+)-ATPase of sarcoplasmic reticulum: effects on interactions between ATPase molecules. Biochim Biophys Acta. 1990;1023:147-67 pubmed
	Molnar E, Varga S, Jona I, Seidler N, Martonosi A. Immunological relatedness of the sarcoplasmic reticulum Ca(2+)-ATPase and the Na+,K(+)-ATPase. Biochim Biophys Acta. 1992;1103:281-95 pubmed

image

image 1 :

Immunofluorescent analysis of SERCA1 ATPase using Anti-SERCA1 ATPase Monoclonal Antibody (VE121G9) (Product# MA3-912) shows staining in A2058 Cells. SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA1 ATPase (Product# MA3-912) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

SERCA1 ATPase Monoclonal Antibody (VE121G9)

Catalog # :

MA3-912

Quantity :

100 µg

Price :

US 412.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Amphibian, Canine, Human, Mouse, Rabbit, Rat

Applications :

Immunocytochemistry: 1:500, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): 1:20, Western Blot: 1 µg/mL

Species :

Amphibian, Canine, Human, Mouse, Rabbit, Rat

Clone :

VE121G9

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.

Immunogen :

Purified rabbit skeletal muscle sarcoplasmic reticulum.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:500, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): 1:20, Western Blot: 1 µg/mL

Aliases :

adult Ca2+ ATPase; ATP2A; ATP2A1; ATP2A3; ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1; ATPase, Ca++ transporting, cardiac muscle, fast twitch 1; ATPase, Ca++ transporting, ubiquitous; Ca2+ ATPase; calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; endoplasmic reticulum class 1/2 Ca(2+) ATPase; neonatal Ca2+ ATPase; sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA; SERCA ATPase; Serca1; SERCA1a; SR Ca(2+)-ATPase 1

more info or order :

Invitrogen product webpage