SERCA1 ATPase Monoclonal Antibody (IIH11) | Invitrogen MA3-911 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

SERCA1 ATPase Monoclonal Antibody (IIH11)

catalog :

MA3-911

quantity :

100 µL

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

IIH11

reactivity :

guinea pig, human, mouse, rat, dogs, domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 90

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:1000; loading ...	Laitila J, McNamara E, Wingate C, Goullee H, Ross J, Taylor R, et al. Nebulin nemaline myopathy recapitulated in a compound heterozygous mouse model with both a missense and a nonsense mutation in Neb. Acta Neuropathol Commun. 2020;8:18 pubmed publisher
western blot; mouse; loading ...; fig 5a	Eshima H, Tamura Y, Kakehi S, Kurebayashi N, Murayama T, Nakamura K, et al. Long-term, but not short-term high-fat diet induces fiber composition changes and impaired contractile force in mouse fast-twitch skeletal muscle. Physiol Rep. 2017;5: pubmed publisher
western blot; mouse; 1:1000; loading ...; fig s2f	Lee C, Hanna A, Wang H, Dagnino Acosta A, Joshi A, Knoblauch M, et al. A chemical chaperone improves muscle function in mice with a RyR1 mutation. Nat Commun. 2017;8:14659 pubmed publisher
western blot; rat; loading ...; fig 2e	Himori K, Abe M, Tatebayashi D, Lee J, Westerblad H, Lanner J, et al. Superoxide dismutase/catalase mimetic EUK-134 prevents diaphragm muscle weakness in monocrotalin-induced pulmonary hypertension. PLoS ONE. 2017;12:e0169146 pubmed publisher
western blot; rat; 1:5000; loading ...; fig 4a	Kanzaki K, Watanabe D, Kuratani M, Yamada T, Matsunaga S, Wada M. Role of calpain in eccentric contraction-induced proteolysis of Ca2+-regulatory proteins and force depression in rat fast-twitch skeletal muscle. J Appl Physiol (1985). 2017;122:396-405 pubmed publisher
western blot; rat; 1:2000; fig 3	de Andrade P, Neff L, Strosova M, Arsenijevic D, Patthey Vuadens O, Scapozza L, et al. Caloric restriction induces energy-sparing alterations in skeletal muscle contraction, fiber composition and local thyroid hormone metabolism that persist during catch-up fat upon refeeding. Front Physiol. 2015;6:254 pubmed publisher
western blot; domestic rabbit	Desmond P, Muriel J, Markwardt M, Rizzo M, Bloch R. Identification of Small Ankyrin 1 as a Novel Sarco(endo)plasmic Reticulum Ca2+-ATPase 1 (SERCA1) Regulatory Protein in Skeletal Muscle. J Biol Chem. 2015;290:27854-67 pubmed publisher
western blot; rat; fig 1	Yamada T, Abe M, Lee J, Tatebayashi D, Himori K, Kanzaki K, et al. Muscle dysfunction associated with adjuvant-induced arthritis is prevented by antioxidant treatment. Skelet Muscle. 2015;5:20 pubmed publisher
western blot; rat	Ferretti R, Marques M, Khurana T, Santo Neto H. Expression of calcium-buffering proteins in rat intrinsic laryngeal muscles. Physiol Rep. 2015;3: pubmed publisher
western blot; mouse	Van B, Nishi M, Komazaki S, Ichimura A, Kakizawa S, Nakanaga K, et al. Mitsugumin 56 (hedgehog acyltransferase-like) is a sarcoplasmic reticulum-resident protein essential for postnatal muscle maturation. FEBS Lett. 2015;589:1095-104 pubmed publisher
western blot; rat; 1:1000; fig 2	Ramos S, Macpherson R, Turnbull P, Bott K, LeBlanc P, Ward W, et al. Higher PLIN5 but not PLIN3 content in isolated skeletal muscle mitochondria following acute in vivo contraction in rat hindlimb. Physiol Rep. 2014;2: pubmed publisher
western blot; mouse; 1:1000	Burr A, Millay D, Goonasekera S, Park K, Sargent M, Collins J, et al. Na+ dysregulation coupled with Ca2+ entry through NCX1 promotes muscular dystrophy in mice. Mol Cell Biol. 2014;34:1991-2002 pubmed publisher
immunocytochemistry; mouse; 1:100	Tajika Y, Takahashi M, Khairani A, Ueno H, Murakami T, Yorifuji H. Vesicular transport system in myotubes: ultrastructural study and signposting with vesicle-associated membrane proteins. Histochem Cell Biol. 2014;141:441-54 pubmed publisher
immunohistochemistry; mouse; 1:15	Luo Y, Johnsen R, Griffiths L, Needham M, Fabian V, Fletcher S, et al. Primary over-expression of A?PP in muscle does not lead to the development of inclusion body myositis in a new lineage of the MCK-A?PP transgenic mouse. Int J Exp Pathol. 2013;94:418-25 pubmed publisher
western blot; human; 1:1000	Kiyonaka S, Kajimoto T, Sakaguchi R, Shinmi D, Omatsu Kanbe M, Matsuura H, et al. Genetically encoded fluorescent thermosensors visualize subcellular thermoregulation in living cells. Nat Methods. 2013;10:1232-8 pubmed publisher
western blot; rat; 1:2500	Eshima H, Tanaka Y, Sonobe T, Inagaki T, Nakajima T, Poole D, et al. In vivo imaging of intracellular Ca2+ after muscle contractions and direct Ca2+ injection in rat skeletal muscle in diabetes. Am J Physiol Regul Integr Comp Physiol. 2013;305:R610-8 pubmed publisher
immunohistochemistry; rat; 1:500	Shortt C, Fredsted A, Bradford A, O Halloran K. Diaphragm muscle remodeling in a rat model of chronic intermittent hypoxia. J Histochem Cytochem. 2013;61:487-99 pubmed publisher
immunohistochemistry; mouse western blot; mouse	Amoasii L, Hnia K, Chicanne G, Brech A, Cowling B, Müller M, et al. Myotubularin and PtdIns3P remodel the sarcoplasmic reticulum in muscle in vivo. J Cell Sci. 2013;126:1806-19 pubmed publisher
western blot; rat	Fajardo V, McMeekin L, Basic A, Lamb G, Murphy R, Leblanc P. Isolation of sarcolemmal plasma membranes by mechanically skinning rat skeletal muscle fibers for phospholipid analysis. Lipids. 2013;48:421-30 pubmed publisher
western blot; mouse; 1:2000	Dorchies O, Reutenauer Patte J, Dahmane E, Ismail H, Petermann O, Patthey Vuadens O, et al. The anticancer drug tamoxifen counteracts the pathology in a mouse model of duchenne muscular dystrophy. Am J Pathol. 2013;182:485-504 pubmed
western blot; domestic rabbit; 1:5000	Prasad A, Inesi G. Regulation and rate limiting mechanisms of Ca2+ ATPase (SERCA2) expression in cardiac myocytes. Mol Cell Biochem. 2012;361:85-96 pubmed publisher
western blot; rat; 1:500	Furieri L, Fioresi M, Junior R, Bartolome M, Fernandes A, Cachofeiro V, et al. Exposure to low mercury concentration in vivo impairs myocardial contractile function. Toxicol Appl Pharmacol. 2011;255:193-9 pubmed publisher
western blot; mouse; 1:2500	Goonasekera S, Lam C, Millay D, Sargent M, Hajjar R, Kranias E, et al. Mitigation of muscular dystrophy in mice by SERCA overexpression in skeletal muscle. J Clin Invest. 2011;121:1044-52 pubmed publisher
immunoprecipitation; mouse western blot; mouse	Arruda A, Milanski M, Coope A, Torsoni A, Ropelle E, Carvalho D, et al. Low-grade hypothalamic inflammation leads to defective thermogenesis, insulin resistance, and impaired insulin secretion. Endocrinology. 2011;152:1314-26 pubmed publisher
immunohistochemistry; mouse	Toussaint A, Cowling B, Hnia K, Mohr M, Oldfors A, Schwab Y, et al. Defects in amphiphysin 2 (BIN1) and triads in several forms of centronuclear myopathies. Acta Neuropathol. 2011;121:253-66 pubmed publisher
western blot; mouse	Zhao X, Min C, Ko J, Parness J, Kim D, Weisleder N, et al. Increased store-operated Ca2+ entry in skeletal muscle with reduced calsequestrin-1 expression. Biophys J. 2010;99:1556-64 pubmed publisher
western blot; rat	Zeiger U, Mitchell C, Khurana T. Superior calcium homeostasis of extraocular muscles. Exp Eye Res. 2010;91:613-22 pubmed publisher
western blot; domestic rabbit	Daiho T, Danko S, Yamasaki K, Suzuki H. Stable structural analog of Ca2+-ATPase ADP-insensitive phosphoenzyme with occluded Ca2+ formed by elongation of A-domain/M1'-linker and beryllium fluoride binding. J Biol Chem. 2010;285:24538-47 pubmed publisher
western blot; mouse; 1:2500	Ferreira J, Bacurau A, Bueno C, Cunha T, Tanaka L, Jardim M, et al. Aerobic exercise training improves Ca2+ handling and redox status of skeletal muscle in mice. Exp Biol Med (Maywood). 2010;235:497-505 pubmed publisher
immunohistochemistry; human; 1:500	Di Blasi C, Blasevich F, Bellafiore E, Mottarelli E, Gibertini S, Zanotti S, et al. Calsequestrin and junctin immunoreactivity in hexagonally cross-linked tubular arrays myopathy. Neuromuscul Disord. 2010;20:326-9 pubmed publisher
immunohistochemistry; rat	de Meis L, Ketzer L, da Costa R, de Andrade I, Benchimol M. Fusion of the endoplasmic reticulum and mitochondrial outer membrane in rats brown adipose tissue: activation of thermogenesis by Ca2+. PLoS ONE. 2010;5:e9439 pubmed publisher
western blot; rat; 1:5000	Aguiar C, Andrade V, Gomes E, Alves M, Ladeira M, Pinheiro A, et al. Succinate modulates Ca(2+) transient and cardiomyocyte viability through PKA-dependent pathway. Cell Calcium. 2010;47:37-46 pubmed publisher
immunocytochemistry; mouse immunohistochemistry; mouse western blot; mouse	Ferretti R, Marques M, Pertille A, Santo Neto H. Sarcoplasmic-endoplasmic-reticulum Ca2+-ATPase and calsequestrin are overexpressed in spared intrinsic laryngeal muscles of dystrophin-deficient mdx mice. Muscle Nerve. 2009;39:609-15 pubmed publisher
immunohistochemistry; rat; 1:1,000	Bleunven C, Treves S, Jinyu X, Leo E, Ronjat M, De Waard M, et al. SRP-27 is a novel component of the supramolecular signalling complex involved in skeletal muscle excitation-contraction coupling. Biochem J. 2008;411:343-9 pubmed
western blot; domestic rabbit	Inesi G, Lewis D, Toyoshima C, Hirata A, de Meis L. Conformational fluctuations of the Ca2+-ATPase in the native membrane environment. Effects of pH, temperature, catalytic substrates, and thapsigargin. J Biol Chem. 2008;283:1189-96 pubmed publisher
western blot; domestic rabbit	Donoghue P, Doran P, Wynne K, Pedersen K, Dunn M, Ohlendieck K. Proteomic profiling of chronic low-frequency stimulated fast muscle. Proteomics. 2007;7:3417-30 pubmed
western blot; rat; 1:2500	Talmadge R, Paalani M. Sarco(endo)plasmic reticulum calcium pump isoforms in paralyzed rat slow muscle. Biochim Biophys Acta. 2007;1770:1187-93 pubmed
western blot; rat	Arruda A, Nigro M, Oliveira G, de Meis L. Thermogenic activity of Ca2+-ATPase from skeletal muscle heavy sarcoplasmic reticulum: the role of ryanodine Ca2+ channel. Biochim Biophys Acta. 2007;1768:1498-505 pubmed
immunocytochemistry; mouse western blot; mouse; 1:20000	Stenoien D, Knyushko T, Londono M, Opresko L, Mayer M, Brady S, et al. Cellular trafficking of phospholamban and formation of functional sarcoplasmic reticulum during myocyte differentiation. Am J Physiol Cell Physiol. 2007;292:C2084-94 pubmed
western blot; rat	Mulvey C, Harno E, Keenan A, Ohlendieck K. Expression of the skeletal muscle dystrophin-dystroglycan complex and syntrophin-nitric oxide synthase complex is severely affected in the type 2 diabetic Goto-Kakizaki rat. Eur J Cell Biol. 2005;84:867-83 pubmed
western blot; domestic rabbit	Donoghue P, Doran P, Dowling P, Ohlendieck K. Differential expression of the fast skeletal muscle proteome following chronic low-frequency stimulation. Biochim Biophys Acta. 2005;1752:166-76 pubmed
immunocytochemistry; rat western blot; rat	de Meis L, Oliveira G, Arruda A, Santos R, Costa R, Benchimol M. The thermogenic activity of rat brown adipose tissue and rabbit white muscle Ca2+-ATPase. IUBMB Life. 2005;57:337-45 pubmed
western blot; mouse	Perez C, Lopez J, Allen P. Expression levels of RyR1 and RyR3 control resting free Ca2+ in skeletal muscle. Am J Physiol Cell Physiol. 2005;288:C640-9 pubmed
	Yamada T, Himori K, Tatebayashi D, Yamada R, Ashida Y, Imai T, et al. Electrical Stimulation Prevents Preferential Skeletal Muscle Myosin Loss in Steroid-Denervation Rats. Front Physiol. 2018;9:1111 pubmed publisher
	Tinklenberg J, Siebers E, Beatka M, Meng H, Yang L, Zhang Z, et al. Myostatin inhibition using mRK35 produces skeletal muscle growth and tubular aggregate formation in wild type and TgACTA1D286G nemaline myopathy mice. Hum Mol Genet. 2018;27:638-648 pubmed publisher
	Desmond P, Labuza A, Muriel J, Markwardt M, Mancini A, Rizzo M, et al. Interactions between small ankyrin 1 and sarcolipin coordinately regulate activity of the sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA1). J Biol Chem. 2017;292:10961-10972 pubmed publisher
	Lee C, Dagnino Acosta A, Yarotskyy V, Hanna A, Lyfenko A, Knoblauch M, et al. Ca(2+) permeation and/or binding to CaV1.1 fine-tunes skeletal muscle Ca(2+) signaling to sustain muscle function. Skelet Muscle. 2015;5:4 pubmed publisher
	Chen Y, Xue S, Zou J, Lopez J, Yang J, Perez C. Myoplasmic resting Ca2+ regulation by ryanodine receptors is under the control of a novel Ca2+-binding region of the receptor. Biochem J. 2014;460:261-71 pubmed publisher
	McKeehen J, Novotny S, Baltgalvis K, Call J, Nuckley D, Lowe D. Adaptations of mouse skeletal muscle to low-intensity vibration training. Med Sci Sports Exerc. 2013;45:1051-9 pubmed publisher
	Al Qusairi L, Weiss N, Toussaint A, Berbey C, Messaddeq N, Kretz C, et al. T-tubule disorganization and defective excitation-contraction coupling in muscle fibers lacking myotubularin lipid phosphatase. Proc Natl Acad Sci U S A. 2009;106:18763-8 pubmed publisher
	Buj Bello A, Fougerousse F, Schwab Y, Messaddeq N, Spehner D, Pierson C, et al. AAV-mediated intramuscular delivery of myotubularin corrects the myotubular myopathy phenotype in targeted murine muscle and suggests a function in plasma membrane homeostasis. Hum Mol Genet. 2008;17:2132-43 pubmed publisher
	Tadini Buoninsegni F, Bartolommei G, Moncelli M, Tal D, Lewis D, Inesi G. Effects of high-affinity inhibitors on partial reactions, charge movements, and conformational States of the Ca2+ transport ATPase (sarco-endoplasmic reticulum Ca2+ ATPase). Mol Pharmacol. 2008;73:1134-40 pubmed publisher
	Ukropec J, Anunciado R, Ravussin Y, Hulver M, Kozak L. UCP1-independent thermogenesis in white adipose tissue of cold-acclimated Ucp1-/- mice. J Biol Chem. 2006;281:31894-908 pubmed
	de Meis L, Arruda A, da Costa R, Benchimol M. Identification of a Ca2+-ATPase in brown adipose tissue mitochondria: regulation of thermogenesis by ATP and Ca2+. J Biol Chem. 2006;281:16384-90 pubmed
	Hua S, Xu C, Ma H, Inesi G. Interference with phosphoenzyme isomerization and inhibition of the sarco-endoplasmic reticulum Ca2+ ATPase by 1,3-dibromo-2,4,6-tris(methylisothiouronium) benzene. J Biol Chem. 2005;280:17579-83 pubmed
	Doran P, Dowling P, Lohan J, McDonnell K, Poetsch S, Ohlendieck K. Subproteomics analysis of Ca+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle. Eur J Biochem. 2004;271:3943-52 pubmed
	Dowling P, Doran P, Ohlendieck K. Drastic reduction of sarcalumenin in Dp427 (dystrophin of 427 kDa)-deficient fibres indicates that abnormal calcium handling plays a key role in muscular dystrophy. Biochem J. 2004;379:479-88 pubmed
	Kjellgren D, Ryan M, Ohlendieck K, Thornell L, Pedrosa Domellöf F. Sarco(endo)plasmic reticulum Ca2+ ATPases (SERCA1 and -2) in human extraocular muscles. Invest Ophthalmol Vis Sci. 2003;44:5057-62 pubmed
	Lees S, Williams J. Skeletal muscle sarcoplasmic reticulum glycogen status influences Ca2+ uptake supported by endogenously synthesized ATP. Am J Physiol Cell Physiol. 2004;286:C97-104 pubmed
	de Meis L. Brown adipose tissue Ca2+-ATPase: uncoupled ATP hydrolysis and thermogenic activity. J Biol Chem. 2003;278:41856-61 pubmed
	Arruda A, da Silva W, Carvalho D, de Meis L. Hyperthyroidism increases the uncoupled ATPase activity and heat production by the sarcoplasmic reticulum Ca2+-ATPase. Biochem J. 2003;375:753-60 pubmed
	Mulvey C, Ohlendieck K. Use of continuous-elution gel electrophoresis as a preparative tool for blot overlay analysis. Anal Biochem. 2003;319:122-30 pubmed
	Dowling P, Lohan J, Ohlendieck K. Comparative analysis of Dp427-deficient mdx tissues shows that the milder dystrophic phenotype of extraocular and toe muscle fibres is associated with a persistent expression of beta-dystroglycan. Eur J Cell Biol. 2003;82:222-30 pubmed
	Kim D, Zhu J, Kozyak B, Burkman J, Rubinstein N, Lankford E, et al. Myosin heavy chain and physiological adaptation of the rat diaphragm in elastase-induced emphysema. Respir Res. 2003;4:1 pubmed
	O Reilly C, Pette D, Ohlendieck K. Increased expression of the nicotinic acetylcholine receptor in stimulated muscle. Biochem Biophys Res Commun. 2003;300:585-91 pubmed
	Schertzer J, Green H, Duhamel T, Tupling A. Mechanisms underlying increases in SR Ca2+-ATPase activity after exercise in rat skeletal muscle. Am J Physiol Endocrinol Metab. 2003;284:E597-610 pubmed
	Glover L, Quinn S, Ryan M, Pette D, Ohlendieck K. Supramolecular calsequestrin complex. Eur J Biochem. 2002;269:4607-16 pubmed
	McGowan T, Madesh M, Zhu Y, Wang L, Russo M, Deelman L, et al. TGF-beta-induced Ca(2+) influx involves the type III IP(3) receptor and regulates actin cytoskeleton. Am J Physiol Renal Physiol. 2002;282:F910-20 pubmed
	Gagelin C, Constantin B, Deprette C, Ludosky M, Recouvreur M, Cartaud J, et al. Identification of Ank(G107), a muscle-specific ankyrin-G isoform. J Biol Chem. 2002;277:12978-87 pubmed
	Culligan K, Banville N, Dowling P, Ohlendieck K. Drastic reduction of calsequestrin-like proteins and impaired calcium binding in dystrophic mdx muscle. J Appl Physiol (1985). 2002;92:435-45 pubmed
	Spangenburg E, Lees S, Otis J, Musch T, Talmadge R, Williams J. Effects of moderate heart failure and functional overload on rat plantaris muscle. J Appl Physiol (1985). 2002;92:18-24 pubmed
	Glover L, Culligan K, Cala S, Mulvey C, Ohlendieck K. Calsequestrin binds to monomeric and complexed forms of key calcium-handling proteins in native sarcoplasmic reticulum membranes from rabbit skeletal muscle. Biochim Biophys Acta. 2001;1515:120-32 pubmed
	Froemming G, Ohlendieck K. Native skeletal muscle dihydropyridine receptor exists as a supramolecular triad complex. Cell Mol Life Sci. 2001;58:312-20 pubmed
	Cho J, Bandyopadhyay J, Lee J, Park C, Ahnn J. Two isoforms of sarco/endoplasmic reticulum calcium ATPase (SERCA) are essential in Caenorhabditis elegans. Gene. 2000;261:211-9 pubmed
	Harmon S, Froemming G, Leisner E, Pette D, Ohlendieck K. Low-frequency stimulation of fast muscle affects the abundance of Ca(2+)-ATPase but not its oligomeric status. J Appl Physiol (1985). 2001;90:371-9 pubmed
	Froemming G, Murray B, Harmon S, Pette D, Ohlendieck K. Comparative analysis of the isoform expression pattern of Ca(2+)-regulatory membrane proteins in fast-twitch, slow-twitch, cardiac, neonatal and chronic low-frequency stimulated muscle fibers. Biochim Biophys Acta. 2000;1466:151-68 pubmed
	Hirata Y, Nakahata N, Ohizumi Y. Identification of a 97-kDa mastoparan-binding protein involving in Ca(2+) release from skeletal muscle sarcoplasmic reticulum. Mol Pharmacol. 2000;57:1235-42 pubmed
	Flucher B, Conti A, Takeshima H, Sorrentino V. Type 3 and type 1 ryanodine receptors are localized in triads of the same mammalian skeletal muscle fibers. J Cell Biol. 1999;146:621-30 pubmed
	Viner R, Ferrington D, Williams T, Bigelow D, SCHONEICH C. Protein modification during biological aging: selective tyrosine nitration of the SERCA2a isoform of the sarcoplasmic reticulum Ca2+-ATPase in skeletal muscle. Biochem J. 1999;340 ( Pt 3):657-69 pubmed
	Buck E, Nguyen H, Pessah I, Allen P. Dyspedic mouse skeletal muscle expresses major elements of the triadic junction but lacks detectable ryanodine receptor protein and function. J Biol Chem. 1997;272:7360-7 pubmed
	Zhou D, Birkenmeier C, Williams M, Sharp J, Barker J, Bloch R. Small, membrane-bound, alternatively spliced forms of ankyrin 1 associated with the sarcoplasmic reticulum of mammalian skeletal muscle. J Cell Biol. 1997;136:621-31 pubmed
	Knudson C, Stang K, Jorgensen A, Campbell K. Biochemical characterization of ultrastructural localization of a major junctional sarcoplasmic reticulum glycoprotein (triadin). J Biol Chem. 1993;268:12637-45 pubmed
	Guo W, Jorgensen A, Campbell K. Characterization and ultrastructural localization of a novel 90-kDa protein unique to skeletal muscle junctional sarcoplasmic reticulum. J Biol Chem. 1994;269:28359-65 pubmed
	Hu P, Yin C, Zhang K, Wright L, Nixon T, Wechsler A, et al. Transcriptional regulation of phospholamban gene and translational regulation of SERCA2 gene produces coordinate expression of these two sarcoplasmic reticulum proteins during skeletal muscle phenotype switching. J Biol Chem. 1995;270:11619-22 pubmed
	Jorgensen A, Arnold W, Pepper D, Kahl S, Mandel F, Campbell K. A monoclonal antibody to the Ca2+-ATPase of cardiac sarcoplasmic reticulum cross-reacts with slow type I but not with fast type II canine skeletal muscle fibers: an immunocytochemical and immunochemical study. Cell Motil Cytoskeleton. 1988;9:164-74 pubmed
	Briggs F, Lee K, Feher J, Wechsler A, Ohlendieck K, Campbell K. Ca-ATPase isozyme expression in sarcoplasmic reticulum is altered by chronic stimulation of skeletal muscle. FEBS Lett. 1990;259:269-72 pubmed
	Ohlendieck K, Ervasti J, Snook J, Campbell K. Dystrophin-glycoprotein complex is highly enriched in isolated skeletal muscle sarcolemma. J Cell Biol. 1991;112:135-48 pubmed
	Molnar E, Seidler N, Jona I, Martonosi A. The binding of monoclonal and polyclonal antibodies to the Ca2(+)-ATPase of sarcoplasmic reticulum: effects on interactions between ATPase molecules. Biochim Biophys Acta. 1990;1023:147-67 pubmed
	Ohlendieck K, Briggs F, Lee K, Wechsler A, Campbell K. Analysis of excitation-contraction-coupling components in chronically stimulated canine skeletal muscle. Eur J Biochem. 1991;202:739-47 pubmed
	Molnar E, Varga S, Jona I, Seidler N, Martonosi A. Immunological relatedness of the sarcoplasmic reticulum Ca(2+)-ATPase and the Na+,K(+)-ATPase. Biochim Biophys Acta. 1992;1103:281-95 pubmed

image

image 1 :

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse lymph node tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing SERCA1 ATPase (MA3-911) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

image 2 :

Immunofluorescent analysis of SERCA1 ATPase using Anti-SERCA1 ATPase Monoclonal Antibody (IIH11) (Product# MA3-911) shows staining in C6 Cells. SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA1 ATPase (Product# MA3-911) at a dilution of 1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

image 3 :

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing SERCA1 ATPase (MA3-911) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

product information

Product Type :

Antibody

Product Name :

SERCA1 ATPase Monoclonal Antibody (IIH11)

Catalog # :

MA3-911

Quantity :

100 µL

Price :

US 399.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Canine, Guinea Pig, Human, Mouse, Rabbit, Rat

Applications :

Immunocytochemistry: 1:500, Immunohistochemistry (Frozen): 1:20-200, Western Blot: 1:500-1:5,000

Species :

Canine, Guinea Pig, Human, Mouse, Rabbit, Rat

Clone :

IIH11

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.

Immunogen :

Purified rabbit skeletal muscle sarcoplasmic reticulum.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:500, Immunohistochemistry (Frozen): 1:20-200, Western Blot: 1:500-1:5,000

Aliases :

adult Ca2+ ATPase; ATP2A; ATP2A1; ATP2A3; ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1; ATPase, Ca++ transporting, cardiac muscle, fast twitch 1; ATPase, Ca++ transporting, ubiquitous; Ca2+ ATPase; calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; endoplasmic reticulum class 1/2 Ca(2+) ATPase; neonatal Ca2+ ATPase; sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA; SERCA ATPase; Serca1; SERCA1a; SR Ca(2+)-ATPase 1

more info or order :

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