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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CRABP1 Monoclonal Antibody (C-1)
catalog :
MA3-813
quantity :
100 µL
price :
US 399.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
C-1
reactivity :
human, mouse, rat, chicken, bovine, cat, domestic rabbit
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 16
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000
Honecker F, Rohlfing T, Harder S, Braig M, Gillis A, Glaesener S, et al. Proteome analysis of the effects of all-trans retinoic acid on human germ cell tumor cell lines. J Proteomics. 2014;96:300-13 pubmed publisher
  • immunocytochemistry; human; 1 ug/ml; tbl 1
Hamilton R, Leach L. Isolation and properties of an in vitro human outer blood-retinal barrier model. Methods Mol Biol. 2011;686:401-16 pubmed publisher
  • western blot; mouse
Stavridis M, Collins B, Storey K. Retinoic acid orchestrates fibroblast growth factor signalling to drive embryonic stem cell differentiation. Development. 2010;137:881-90 pubmed publisher
  • western blot; mouse; 1:500
Lane M, Xu J, Wilen E, Sylvester R, Derguini F, Gudas L. LIF removal increases CRABPI and CRABPII transcripts in embryonic stem cells cultured in retinol or 4-oxoretinol. Mol Cell Endocrinol. 2008;280:63-74 pubmed
  • immunohistochemistry; human; 1:250
Hamilton R, Foss A, Leach L. Establishment of a human in vitro model of the outer blood-retinal barrier. J Anat. 2007;211:707-16 pubmed
  • immunohistochemistry; mouse; 1:50
  • western blot; mouse; 1:1000
Tang X, Vivero M, Gudas L. Overexpression of CRABPI in suprabasal keratinocytes enhances the proliferation of epidermal basal keratinocytes in mouse skin topically treated with all-trans retinoic acid. Exp Cell Res. 2008;314:38-51 pubmed
  • immunohistochemistry; chicken; 1:200
  • western blot; chicken; 1:1000
Propping C, Mönig B, Luksch H, Mey J. Distribution of the cellular retinoic acid binding protein CRABP-I in the developing chick optic tectum. Brain Res. 2007;1168:21-31 pubmed
  • western blot; human; fig 3
Erickson R, Dunning L, Olson D, Cohen S, Davis A, Wood W, et al. In cerebrospinal fluid ER chaperones ERp57 and calreticulin bind beta-amyloid. Biochem Biophys Res Commun. 2005;332:50-7 pubmed
Hibbs K, Skubitz K, Pambuccian S, Casey R, Burleson K, Oegema T, et al. Differential gene expression in ovarian carcinoma: identification of potential biomarkers. Am J Pathol. 2004;165:397-414 pubmed
Esteve P, Trousse F, Rodriguez J, Bovolenta P. SFRP1 modulates retina cell differentiation through a beta-catenin-independent mechanism. J Cell Sci. 2003;116:2471-81 pubmed
Mey J, McCaffery P, Klemeit M. Sources and sink of retinoic acid in the embryonic chick retina: distribution of aldehyde dehydrogenase activities, CRABP-I, and sites of retinoic acid inactivation. Brain Res Dev Brain Res. 2001;127:135-48 pubmed
Kelley M, Xu X, Wagner M, Warchol M, Corwin J. The developing organ of Corti contains retinoic acid and forms supernumerary hair cells in response to exogenous retinoic acid in culture. Development. 1993;119:1041-53 pubmed
Kelley M, Turner J, Reh T. Retinoic acid promotes differentiation of photoreceptors in vitro. Development. 1994;120:2091-102 pubmed
Gaur V, De Leeuw A, Milam A, Saari J. Localization of cellular retinoic acid-binding protein to amacrine cells of rat retina. Exp Eye Res. 1990;50:505-11 pubmed
De Leeuw A, Gaur V, Saari J, Milam A. Immunolocalization of cellular retinol-, retinaldehyde- and retinoic acid-binding proteins in rat retina during pre- and postnatal development. J Neurocytol. 1990;19:253-64 pubmed
Milam A, De Leeuw A, Gaur V, Saari J. Immunolocalization of cellular retinoic acid binding protein to Müller cells and/or a subpopulation of GABA-positive amacrine cells in retinas of different species. J Comp Neurol. 1990;296:123-9 pubmed
image
image 1 :
Invitrogen MA3-813 image 1
Immunofluorescent analysis of CRABPI using Anti-CRABPI Monoclonal Antibody (C-1) (Product# MA3-813) shows staining in Hela Cells. CRABPI staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CRABPI (Product# MA3-813) at a dilution of 1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
product information
Product Type :
Antibody
Product Name :
CRABP1 Monoclonal Antibody (C-1)
Catalog # :
MA3-813
Quantity :
100 µL
Price :
US 399.00
Clonality :
Monoclonal
Host :
Mouse
Reactivity :
Bovine, Chicken, Feline, Human, Mouse, Non-human primate, Rabbit, Rat, Reptile
Applications :
ELISA: Assay-dependent, Flow Cytometry: Assay-dependent, Immunocytochemistry: 1:100-1:200, Immunohistochemistry (Frozen): 1:250, Immunohistochemistry (Paraffin): 1:1,000, Western Blot: 1:1,000
Species :
Bovine, Chicken, Feline, Human, Mouse, Non-human primate, Rabbit, Rat, Reptile
Clone :
C-1
Isotype :
IgG2b
Storage :
-20° C, Avoid Freeze/Thaw Cycles
Description :
This gene encodes a specific binding protein for a vitamin A family member and is thought to play an important role in retinoic acid-mediated differentiation and proliferation processes. It is structurally similar to the cellular retinol-binding proteins, but binds only retinoic acid at specific sites within the nucleus, which may contribute to vitamin A-directed differentiation in epithelial tissue.
Immunogen :
Oxidized CRABPI from bovine retina.
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-dependent, Flow Cytometry: Assay-dependent, Immunocytochemistry: 1:100-1:200, Immunohistochemistry (Frozen): 1:250, Immunohistochemistry (Paraffin): 1:1,000, Western Blot: 1:1,000
Aliases :
AI326249; cCRABP-I; cellular retinoic acid binding protein 1; cellular retinoic acid binding protein I; cellular retinoic acid binding protein I (CRABP-I) homologue; cellular retinoic acid-binding protein 1; Cellular retinoic acid-binding protein I; cellular retinoic-acid-binding protein; CRABP; Crabp1; Crabp-1; CrabpI; CRABP-I; RBP5; Rbp-5; retinoic acid-binding protein
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA