Vimentin Monoclonal Antibody (J144) | Invitrogen MA3-745 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Vimentin Monoclonal Antibody (J144)

catalog :

MA3-745

quantity :

200 µL

price :

US 425.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

J144

reactivity :

human, mouse, rat, dogs

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, other

more info or order :

Invitrogen product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
other; human; loading ...; tbl 5.1	Hu Y, Janitz M. High-throughput subcellular protein localization using transfected-cell arrays. Subcellular protein localization using cell arrays. Methods Mol Biol. 2011;706:53-72 pubmed publisher
immunocytochemistry; mouse	Yanni S, McCollum G, Penn J. Genetic deletion of COX-2 diminishes VEGF production in mouse retinal Müller cells. Exp Eye Res. 2010;91:34-41 pubmed publisher
western blot; human; 1:1000	Sander T, Ou J, Densmore J, Kaul S, Matus I, Twigger S, et al. Protein composition of plasminogen activator inhibitor type 1-derived endothelial microparticles. Shock. 2008;29:504-11 pubmed
western blot; human	Brasaemle D, Wolins N. Isolation of lipid droplets from cells by density gradient centrifugation. Curr Protoc Cell Biol. 2006;Chapter 3:Unit 3.15 pubmed publisher
western blot; mouse	Gramolini A, Kislinger T, Alikhani Koopaei R, Fong V, Thompson N, Isserlin R, et al. Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses. Mol Cell Proteomics. 2008;7:519-33 pubmed
	Lin F, Zeng Z, Song Y, Li L, Wu Z, Zhang X, et al. YBX-1 mediated sorting of miR-133 into hypoxia/reoxygenation-induced EPC-derived exosomes to increase fibroblast angiogenesis and MEndoT. Stem Cell Res Ther. 2019;10:263 pubmed publisher
	Moore L, Keller L, Bushart D, Delatorre R, Li D, McLoughlin H, et al. Antisense oligonucleotide therapy rescues aggresome formation in a novel spinocerebellar ataxia type 3 human embryonic stem cell line. Stem Cell Res. 2019;39:101504 pubmed publisher
	Chu X, Xue Y, Huo X, Wei J, Chen Y, Han R, et al. Establishment and characterization of a novel cell line (cc‑006cpm8) of moderately/poorly differentiated colorectal adenocarcinoma derived from a primary tumor of a patient. Int J Oncol. 2019;55:243-256 pubmed publisher
	Singh M, Tian X, Donnenberg V, Watson A, Zhang J, Stabile L, et al. Targeting the Temporal Dynamics of Hypoxia-Induced Tumor-Secreted Factors Halts Tumor Migration. Cancer Res. 2019;79:2962-2977 pubmed publisher
	Ratnayake W, Apostolatos C, Apostolatos A, Schutte R, Huynh M, Ostrov D, et al. Oncogenic PKC-ι activates Vimentin during epithelial-mesenchymal transition in melanoma; a study based on PKC-ι and PKC-ζ specific inhibitors. Cell Adh Migr. 2018;12:447-463 pubmed publisher
	Ratnayake W, Apostolatos A, Ostrov D, Acevedo Duncan M. Two novel atypical PKC inhibitors; ACPD and DNDA effectively mitigate cell proliferation and epithelial to mesenchymal transition of metastatic melanoma while inducing apoptosis. Int J Oncol. 2017;51:1370-1382 pubmed publisher
	He X, Qian Y, Cai H, Yang S, Cai J, Wang Z. RhoC is essential in TGF-Î²1 induced epithelial-mesenchymal transition in cervical cancer cells. Oncol Lett. 2015;10:985-989 pubmed
	Takenouchi H, Kiyokawa N, Taguchi T, Matsui J, Katagiri Y, Okita H, et al. Shiga toxin binding to globotriaosyl ceramide induces intracellular signals that mediate cytoskeleton remodeling in human renal carcinoma-derived cells. J Cell Sci. 2004;117:3911-22 pubmed

image

image 1 :

Immunofluorescent analysis of Vimentin using Vimentin Monoclonal antibody (J144) (Product# MA3-745) shows staining in HEK293 cells. Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin (Product# MA3-745) at a dilution of 1:100-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

image 2 :

Immunohistochemistry was performed on biopsies of deparaffinized Human melanoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Vimentin (MA3-745) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

image 3 :

Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Vimentin (MA3-745) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

product information

Product Type :

Antibody

Product Name :

Vimentin Monoclonal Antibody (J144)

Catalog # :

MA3-745

Quantity :

200 µL

Price :

US 425.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Canine, Human, Mouse, Rat

Applications :

Immunocytochemistry: 1:20, Immunohistochemistry (Paraffin): 1:20, Western Blot: 1:1,000

Species :

Canine, Human, Mouse, Rat

Clone :

J144

Isotype :

IgM

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

Immunogen :

Human rhabdosarcoma cell line JR1.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:20, Immunohistochemistry (Paraffin): 1:20, Western Blot: 1:1,000

Aliases :

cb28; class-III intermediate microfilament; CTRCT30; epididymis luminal protein 113; FLJ36605; HEL113; intermediate filament; intermediate filament protein; RP11-124N14.1; similar to vimentin; VIM; VIME; Vimentin; vimentin C-terminus

more info or order :

Invitrogen product webpage