p23 Monoclonal Antibody (JJ3) | Invitrogen MA3-414 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

p23 Monoclonal Antibody (JJ3)

catalog :

MA3-414

quantity :

100 µL

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

JJ3

reactivity :

guinea pig, human, mouse, rat, chicken, domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, RNA immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 40

Published Application/Species/Sample/Dilution	Reference
western blot; human	Knapp R, Wong M, Kollmannsberger L, Gassen N, Kretzschmar A, Zschocke J, et al. Hsp70 cochaperones HspBP1 and BAG-1M differentially regulate steroid hormone receptor function. PLoS ONE. 2014;9:e85415 pubmed publisher
western blot; human; fig 3	Caligiuri I, Toffoli G, Giordano A, Rizzolio F. pRb controls estrogen receptor alpha protein stability and activity. Oncotarget. 2013;4:875-83 pubmed
immunoprecipitation; human western blot; human; fig 7b	Pare J, LaPointe P, Hobman T. Hsp90 cochaperones p23 and FKBP4 physically interact with hAgo2 and activate RNA interference-mediated silencing in mammalian cells. Mol Biol Cell. 2013;24:2303-10 pubmed publisher
western blot; human	Donnelly B, Needham P, Snyder A, Roy A, Khadem S, Brodsky J, et al. Hsp70 and Hsp90 multichaperone complexes sequentially regulate thiazide-sensitive cotransporter endoplasmic reticulum-associated degradation and biogenesis. J Biol Chem. 2013;288:13124-35 pubmed publisher
western blot; human; 1:200	Nguyen P, Wang D, Wang Y, Li Y, Uchizono J, Chan W. p23 co-chaperone protects the aryl hydrocarbon receptor from degradation in mouse and human cell lines. Biochem Pharmacol. 2012;84:838-50 pubmed publisher
western blot; human	Elkoreh G, Blais V, Béliveau E, Guillemette G, Denault J. Type 1 inositol-1,4,5-trisphosphate receptor is a late substrate of caspases during apoptosis. J Cell Biochem. 2012;113:2775-84 pubmed publisher
western blot; human	Walsh J, Logue S, Lüthi A, Martin S. Caspase-1 promiscuity is counterbalanced by rapid inactivation of processed enzyme. J Biol Chem. 2011;286:32513-24 pubmed publisher
western blot; human; 1:2000	Boucher D, Blais V, Drag M, Denault J. Molecular determinants involved in activation of caspase 7. Biosci Rep. 2011;31:283-94 pubmed publisher
immunohistochemistry; human western blot; human	Simpson N, Lambert W, Watkins R, Giashuddin S, Huang S, Oxelmark E, et al. High levels of Hsp90 cochaperone p23 promote tumor progression and poor prognosis in breast cancer by increasing lymph node metastases and drug resistance. Cancer Res. 2010;70:8446-56 pubmed publisher
immunocytochemistry; mouse western blot; mouse	Quintá H, Maschi D, Gomez Sanchez C, Piwien Pilipuk G, Galigniana M. Subcellular rearrangement of hsp90-binding immunophilins accompanies neuronal differentiation and neurite outgrowth. J Neurochem. 2010;115:716-34 pubmed publisher
western blot; human	Walerych D, Gutkowska M, Klejman M, Wawrzynow B, Tracz Z, Wiech M, et al. ATP binding to Hsp90 is sufficient for effective chaperoning of p53 protein. J Biol Chem. 2010;285:32020-8 pubmed publisher
western blot; mouse western blot; human	Schülke J, Wochnik G, Lang Rollin I, Gassen N, Knapp R, Berning B, et al. Differential impact of tetratricopeptide repeat proteins on the steroid hormone receptors. PLoS ONE. 2010;5:e11717 pubmed publisher
western blot; rat; 1:2000	Martinez J, Zhang Z, Svetlov S, Hayes R, Wang K, Larner S. Calpain and caspase processing of caspase-12 contribute to the ER stress-induced cell death pathway in differentiated PC12 cells. Apoptosis. 2010;15:1480-93 pubmed publisher
western blot; mouse	Caro Maldonado A, Tait S, Ramírez Peinado S, Ricci J, Fabregat I, Green D, et al. Glucose deprivation induces an atypical form of apoptosis mediated by caspase-8 in Bax-, Bak-deficient cells. Cell Death Differ. 2010;17:1335-44 pubmed publisher
western blot; mouse	Galigniana M, Erlejman A, Monte M, Gomez Sanchez C, Piwien Pilipuk G. The hsp90-FKBP52 complex links the mineralocorticoid receptor to motor proteins and persists bound to the receptor in early nuclear events. Mol Cell Biol. 2010;30:1285-98 pubmed publisher
immunoprecipitation; human	Echeverria P, Mazaira G, Erlejman A, Gomez Sanchez C, Piwien Pilipuk G, Galigniana M. Nuclear import of the glucocorticoid receptor-hsp90 complex through the nuclear pore complex is mediated by its interaction with Nup62 and importin beta. Mol Cell Biol. 2009;29:4788-97 pubmed publisher
immunohistochemistry; human	McDowell C, Bryan Sutton R, Obermann W. Expression of Hsp90 chaperone [corrected] proteins in human tumor tissue. Int J Biol Macromol. 2009;45:310-4 pubmed publisher
western blot; human	Inoue S, Browne G, Melino G, Cohen G. Ordering of caspases in cells undergoing apoptosis by the intrinsic pathway. Cell Death Differ. 2009;16:1053-61 pubmed publisher
immunoprecipitation; mouse	Taherian A, Krone P, Ovsenek N. A comparison of Hsp90alpha and Hsp90beta interactions with cochaperones and substrates. Biochem Cell Biol. 2008;86:37-45 pubmed publisher
immunoprecipitation; mouse	Oda T, Hayano T, Miyaso H, Takahashi N, Yamashita T. Hsp90 regulates the Fanconi anemia DNA damage response pathway. Blood. 2007;109:5016-26 pubmed
immunocytochemistry; human western blot; human	Oxelmark E, Roth J, Brooks P, Braunstein S, Schneider R, Garabedian M. The cochaperone p23 differentially regulates estrogen receptor target genes and promotes tumor cell adhesion and invasion. Mol Cell Biol. 2006;26:5205-13 pubmed
western blot; human; 1:1500	Maida M, Hurley S, Daeschner J, Moore A, O Banion M. Cytosolic prostaglandin E2 synthase (cPGES) expression is decreased in discrete cortical regions in psychiatric disease. Brain Res. 2006;1103:164-72 pubmed
western blot; human	Waza M, Adachi H, Katsuno M, Minamiyama M, Sang C, Tanaka F, et al. 17-AAG, an Hsp90 inhibitor, ameliorates polyglutamine-mediated motor neuron degeneration. Nat Med. 2005;11:1088-95 pubmed
	Lei W, Duron D, Stine C, Mishra S, Blagg B, Streicher J. The Alpha Isoform of Heat Shock Protein 90 and the Co-chaperones p23 and Cdc37 Promote Opioid Anti-nociception in the Brain. Front Mol Neurosci. 2019;12:294 pubmed publisher
	Bernadotte A, Kumar R, Winblad B, Pavlov P. In silico identification and biochemical characterization of the human dicarboxylate clamp TPR protein interaction network. FEBS Open Bio. 2018;8:1830-1843 pubmed publisher
	Vottero A, Minari R, Viani I, Tassi F, Bonatti F, Neri T, et al. Evidence for epigenetic abnormalities of the androgen receptor gene in foreskin from children with hypospadias. J Clin Endocrinol Metab. 2011;96:E1953-62 pubmed publisher
	Tokui K, Adachi H, Waza M, Katsuno M, Minamiyama M, Doi H, et al. 17-DMAG ameliorates polyglutamine-mediated motor neuron degeneration through well-preserved proteasome function in an SBMA model mouse. Hum Mol Genet. 2009;18:898-910 pubmed publisher
	Bhangoo M, Tzankov S, Fan A, Dejgaard K, Thomas D, Young J. Multiple 40-kDa heat-shock protein chaperones function in Tom70-dependent mitochondrial import. Mol Biol Cell. 2007;18:3414-28 pubmed
	Pollenz R, Dougherty E. Redefining the role of the endogenous XAP2 and C-terminal hsp70-interacting protein on the endogenous Ah receptors expressed in mouse and rat cell lines. J Biol Chem. 2005;280:33346-56 pubmed
	Meadows J, Pitzer B, Brockman D, Myatt L. Expression and localization of adipophilin and perilipin in human fetal membranes: association with lipid bodies and enzymes involved in prostaglandin synthesis. J Clin Endocrinol Metab. 2005;90:2344-50 pubmed
	Gold B, Zhong Y. FK506 requires stimulation of the extracellular signal-regulated kinase 1/2 and the steroid receptor chaperone protein p23 for neurite elongation. Neurosignals. 2004;13:122-9 pubmed
	Stavreva D, Müller W, Hager G, Smith C, McNally J. Rapid glucocorticoid receptor exchange at a promoter is coupled to transcription and regulated by chaperones and proteasomes. Mol Cell Biol. 2004;24:2682-97 pubmed
	Botos J, Xian W, Smith D, Smith C. Progesterone receptor deficient in chromatin binding has an altered cellular state. J Biol Chem. 2004;279:15231-9 pubmed
	Subbaramaiah K, Yoshimatsu K, Scherl E, Das K, Glazier K, Golijanin D, et al. Microsomal prostaglandin E synthase-1 is overexpressed in inflammatory bowel disease. Evidence for involvement of the transcription factor Egr-1. J Biol Chem. 2004;279:12647-58 pubmed
	Tahbaz N, Carmichael J, Hobman T. GERp95 belongs to a family of signal-transducing proteins and requires Hsp90 activity for stability and Golgi localization. J Biol Chem. 2001;276:43294-9 pubmed
	Felts S, Owen B, Nguyen P, Trepel J, Donner D, Toft D. The hsp90-related protein TRAP1 is a mitochondrial protein with distinct functional properties. J Biol Chem. 2000;275:3305-12 pubmed
	Gilmore R, Coffey M, Lee P. Active participation of Hsp90 in the biogenesis of the trimeric reovirus cell attachment protein sigma1. J Biol Chem. 1998;273:15227-33 pubmed
	Hu J, Toft D, Seeger C. Hepadnavirus assembly and reverse transcription require a multi-component chaperone complex which is incorporated into nucleocapsids. EMBO J. 1997;16:59-68 pubmed
	Johnson J, Beito T, Krco C, Toft D. Characterization of a novel 23-kilodalton protein of unactive progesterone receptor complexes. Mol Cell Biol. 1994;14:1956-63 pubmed
	Johnson J, Toft D. A novel chaperone complex for steroid receptors involving heat shock proteins, immunophilins, and p23. J Biol Chem. 1994;269:24989-93 pubmed

image

image 1 :

Immunofluorescent analysis of p23 using Anti-p23 Monoclonal Antibody (JJ5) (Product# MA3-414) shows staining in C6 Cells. p23 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing p23 (Product# MA3-414) at a dilution of 1:500 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

p23 Monoclonal Antibody (JJ3)

Catalog # :

MA3-414

Quantity :

100 µL

Price :

US 399.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Chicken, Guinea Pig, Human, Mouse, Rabbit, Rat

Applications :

Flow Cytometry: 1/100, Immunocytochemistry: 1:200-1:2,000, Immunohistochemistry (Paraffin): Assay-dependent, Immunoprecipitation: Assay-dependent, RNA Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Species :

Chicken, Guinea Pig, Human, Mouse, Rabbit, Rat

Clone :

JJ3

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Prior to activation, steroid receptors associate with a number of different proteins in both a stable and transient fashion. Steroid receptor complex proteins include heat shock proteins (HSP70 and HSP90), immunosuppressant binding proteins called immunophilins (the FK506 binding proteins, FKBP52 & FKBP54 and the cyclosporin binding protein, CyP-40) and at least three other proteins termed p23, p60 and p48. p23 along with HSP70, HSP90 and p60, combine with progesterone receptor (PR) as members of a transient intermediate complex. Cloned human p23 encodes a protein of 160 amino acids that is highly conserved between species and shows no homology to previously identified proteins. p23 is a highly acidic phosphoprotein with an aspartic acid-rich C-terminal domain and multiple potential phosphorylation sites. In vitro studies have suggested that p23 binds to HSP90 and is necessary for the binding of HSP90 and CyP-40 to PR. While neither its exact function nor mechanism of action have been identified, p23 appears to be an important factor in PR function.

Immunogen :

Recombinant human p23 protein.

Format :

Liquid

Applications w/Dilutions :

Aliases :

5730442A20Rik; Co chaperone p23; cPGES; cytosolic prostaglandin E synthase; cytosolic prostaglandin E2 synthase; Hsp90 co chaperone; Hsp90 co-chaperone; LOC100351480; P23; p23 cochaperone; progesterone receptor; progesterone receptor complex p23; Prostaglandin E synthase 3; prostaglandin E synthase 3 (cytosolic); prostaglandin-E synthase 3; Ptges; PTGES3; ptges3 {ECO:0000250; RGD1561913; sid 3177; Sid3177; Tebp; telomerase binding protein, p23; telomerase-binding protein p23; unactive progesterone receptor, 23 kD; UniProtKB:Q15185}

more info or order :

Invitrogen product webpage