product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Estrogen Receptor alpha Monoclonal Antibody (EVG F9)
catalog :
MA3-310
quantity :
100 µL
price :
US 450.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
EVG F9
reactivity :
human, domestic rabbit
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 9
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; domestic rabbit; 1:150; loading ...; tbl 1
Hernández Aragón L, García Villamar V, Carrasco Ruiz M, Nicolás Toledo L, Ortega A, Cuevas Romero E, et al. Role of Estrogens in the Size of Neuronal Somata of Paravaginal Ganglia in Ovariectomized Rabbits. Biomed Res Int. 2017;2017:2089645 pubmed publisher
  • immunohistochemistry; domestic rabbit
de los Ángeles Carrasco Ruiz M, García Villamar V, López García K, Sánchez García O, Pacheco P, Cuevas E, et al. Aromatase expression is linked to estrogenic sensitivity of periurethral muscles in female rabbits. Cell Biochem Funct. 2015;33:188-95 pubmed publisher
  • western blot; domestic rabbit; 1:1000
Yang X, Chen G, Papp R, DeFranco D, Zeng F, Salama G. Oestrogen upregulates L-type Ca²? channels via oestrogen-receptor- by a regional genomic mechanism in female rabbit hearts. J Physiol. 2012;590:493-508 pubmed publisher
Bhadouria N, Berman A, Wallace J, Holguin N. Raloxifene Stimulates Estrogen Signaling to Protect Against Age- and Sex-Related Intervertebral Disc Degeneration in Mice. Front Bioeng Biotechnol. 2022;10:924918 pubmed publisher
Theodossiou T, Ali M, Grigalavicius M, Grallert B, Dillard P, Schink K, et al. Simultaneous defeat of MCF7 and MDA-MB-231 resistances by a hypericin PDT-tamoxifen hybrid therapy. NPJ Breast Cancer. 2019;5:13 pubmed publisher
Carrasco Ruiz M, Hernández Aragón L, Chávez Ríos J, Rodríguez Antolín J, Pacheco P, Martínez Gómez M, et al. High Estradiol Differentially Affects the Expression of the Glucose Transporter Type 4 in Pelvic Floor Muscles of Rats. Int Neurourol J. 2018;22:161-168 pubmed publisher
Qin S, Ingle J, Liu M, Yu J, Wickerham D, Kubo M, et al. Calmodulin-like protein 3 is an estrogen receptor alpha coregulator for gene expression and drug response in a SNP, estrogen, and SERM-dependent fashion. Breast Cancer Res. 2017;19:95 pubmed publisher
Pavao M, Huang Y, Hafer L, Moreland R, Traish A. Immunodetection of nmt55/p54nrb isoforms in human breast cancer. BMC Cancer. 2001;1:15 pubmed
Joel P, Traish A, Lannigan D. Estradiol-induced phosphorylation of serine 118 in the estrogen receptor is independent of p42/p44 mitogen-activated protein kinase. J Biol Chem. 1998;273:13317-23 pubmed
image
image 1 :
Invitrogen MA3-310 image 1
Immunofluorescent analysis of Estrogen Receptor alpha using Anti-Estrogen Receptor alpha Monoclonal Antibody (EVG F9) (Product# MA3-310) shows staining in Hela Cells. Estrogen Receptor alpha staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Estrogen Receptor alpha (Product# MA3-310) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
product information
Product Type :
Antibody
Product Name :
Estrogen Receptor alpha Monoclonal Antibody (EVG F9)
Catalog # :
MA3-310
Quantity :
100 µL
Price :
US 450.00
Clonality :
Monoclonal
Host :
Mouse
Reactivity :
Human, Rabbit
Applications :
Immunocytochemistry: 1:100-1:200, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:2,000
Species :
Human, Rabbit
Clone :
EVG F9
Isotype :
IgG1
Storage :
-20° C, Avoid Freeze/Thaw Cycles
Description :
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
Immunogen :
Mix of synthetic peptides corresponding to residuesT(140) V R E A G P P A F Y R P N S(154) & E(247) V G M M K G G I R K D R R G G R(263) of human ER alpha.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1:100-1:200, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:2,000
Aliases :
DKFZp686N23123; ER; Er alpha; ER36; ERa; ERalpha; ER-alpha; ERalpha protein; ESR; esr 1; Esr1; esr-1; ESRA; Estr; Estra; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1; estrogen receptor 1 (alpha); estrogen receptor alpha; estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; estrogen receptor alpha variant delta 4; estrogen receptor protein; estrogen receptor, alpha; ESTRR; hER-alpha36; I79_001166; Nr3a1; nuclear receptor; nuclear receptor subfamily 3 group A member 1; RNESTROR; RP1-130E4.1; unnamed protein product
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA