product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Aggrecan Monoclonal Antibody (BC-3)
catalog :
MA3-16888
quantity :
100 µg
price :
US 420.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
BC-3
reactivity :
guinea pig, Lyme disease spirochete, horses, human, mouse, rat, dogs, bovine, pigs , zebrafish , cat, domestic sheep, domestic rabbit, domestic horse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 33
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:200; loading ...; fig 1g
Wu J, Song D, Li Z, Guo B, Xiao Y, Liu W, et al. Immunity-and-matrix-regulatory cells derived from human embryonic stem cells safely and effectively treat mouse lung injury and fibrosis. Cell Res. 2020;30:794-809 pubmed publisher
  • immunohistochemistry; horses; 1:30; loading ...; tbl 1
Kremer A, Ribitsch I, Reboredo J, Dürr J, Egerbacher M, Jenner F, et al. Three-Dimensional Coculture of Meniscal Cells and Mesenchymal Stem Cells in Collagen Type I Hydrogel on a Small Intestinal Matrix-A Pilot Study Toward Equine Meniscus Tissue Engineering. Tissue Eng Part A. 2017;23:390-402 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:100; fig 7
  • immunohistochemistry - paraffin section; pigs ; 1:100; fig 7
Beck E, Barragan M, Tadros M, Gehrke S, Detamore M. Approaching the compressive modulus of articular cartilage with a decellularized cartilage-based hydrogel. Acta Biomater. 2016;38:94-105 pubmed publisher
  • immunohistochemistry - frozen section; pigs ; 1:100; fig 8
Beck E, Barragan M, Libeer T, Kieweg S, Converse G, Hopkins R, et al. Chondroinduction from Naturally Derived Cartilage Matrix: A Comparison Between Devitalized and Decellularized Cartilage Encapsulated in Hydrogel Pastes. Tissue Eng Part A. 2016;22:665-79 pubmed publisher
  • immunohistochemistry - paraffin section; zebrafish ; fig 5
Govindan J, Tun K, Iovine M. Cx43-Dependent Skeletal Phenotypes Are Mediated by Interactions between the Hapln1a-ECM and Sema3d during Fin Regeneration. PLoS ONE. 2016;11:e0148202 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:100; fig 9
  • immunohistochemistry - frozen section; pigs ; 1:100; fig 9
Beck E, Barragan M, Tadros M, Kiyotake E, Acosta F, Kieweg S, et al. Chondroinductive Hydrogel Pastes Composed of Naturally Derived Devitalized Cartilage. Ann Biomed Eng. 2016;44:1863-80 pubmed publisher
  • immunohistochemistry - frozen section; zebrafish ; fig 1c
Govindan J, Iovine M. Dynamic remodeling of the extra cellular matrix during zebrafish fin regeneration. Gene Expr Patterns. 2015;19:21-9 pubmed publisher
  • western blot; human
Wanner J, Subbaiah R, Skomorovska Prokvolit Y, Shishani Y, Boilard E, Mohan S, et al. Proteomic profiling and functional characterization of early and late shoulder osteoarthritis. Arthritis Res Ther. 2013;15:R180 pubmed publisher
  • western blot; Lyme disease spirochete; 2 ug/ml
Russell T, Johnson B. Lyme disease spirochaetes possess an aggrecan-binding protease with aggrecanase activity. Mol Microbiol. 2013;90:228-40 pubmed publisher
  • immunocytochemistry; mouse; 1:200
Hiyama A, Sakai D, Arai F, Nakajima D, Yokoyama K, Mochida J. Effects of a glycogen synthase kinase-3? inhibitor (LiCl) on c-myc protein in intervertebral disc cells. J Cell Biochem. 2011;112:2974-86 pubmed publisher
Nativel F, Smith A, Boulestreau J, L xe9 pine C, Baron J, Marquis M, et al. Micromolding-based encapsulation of mesenchymal stromal cells in alginate for intraarticular injection in osteoarthritis. Mater Today Bio. 2023;19:100581 pubmed publisher
Dalle Carbonare L, Bertacco J, Minoia A, Cominacini M, Bhandary L, Elia R, et al. Modulation of miR-204 Expression during Chondrogenesis. Int J Mol Sci. 2022;23: pubmed publisher
Wang Z, Rao Z, Wang X, Jiang C, Yang Y. circPhc3 sponging microRNA‑93‑3p is involved in the regulation of chondrocyte function by mechanical instability in osteoarthritis. Int J Mol Med. 2022;49: pubmed publisher
Deng X, Chen X, Geng F, Tang X, Li Z, Zhang J, et al. Precision 3D printed meniscus scaffolds to facilitate hMSCs proliferation and chondrogenic differentiation for tissue regeneration. J Nanobiotechnology. 2021;19:400 pubmed publisher
Santamaria S, Martin D, Dong X, Yamamoto K, Apte S, Ahnstr xf6 m J. Post-translational regulation and proteolytic activity of the metalloproteinase ADAMTS8. J Biol Chem. 2021;297:101323 pubmed publisher
Wang X, Wang D, Xia P, Cheng K, Wang Q, Wang X, et al. Ultrasound-targeted simvastatin-loaded microbubble destruction promotes OA cartilage repair by modulating the cholesterol efflux pathway mediated by PPARγ in rabbits. Bone Joint Res. 2021;10:693-703 pubmed publisher
Riveiro A, Amorim S, Solanki A, Costa D, Pires R, Quintero F, et al. Hyaluronic acid hydrogels reinforced with laser spun bioactive glass micro- and nanofibres doped with lithium. Mater Sci Eng C Mater Biol Appl. 2021;126:112124 pubmed publisher
Zhang M, Zhang R, Zheng T, Chen Z, Ji G, Peng F, et al. Xanthohumol Attenuated Inflammation and ECM Degradation by Mediating HO-1/C/EBPβ Pathway in Osteoarthritis Chondrocytes. Front Pharmacol. 2021;12:680585 pubmed publisher
Lin J, Du J, Wu X, Xu C, Liu J, Jiang L, et al. SIRT3 mitigates intervertebral disc degeneration by delaying oxidative stress-induced senescence of nucleus pulposus cells. J Cell Physiol. 2021;236:6441-6456 pubmed publisher
Santamaria S, Cuffaro D, Nuti E, Ciccone L, Tuccinardi T, Liva F, et al. Exosite inhibition of ADAMTS-5 by a glycoconjugated arylsulfonamide. Sci Rep. 2021;11:949 pubmed publisher
Calejo I, Costa Almeida R, Reis R, Gomes M. In vitro temporal HIF-mediated deposition of osteochondrogenic matrix governed by hypoxia and osteogenic factors synergy. J Cell Physiol. 2021;236:3991-4007 pubmed publisher
Jandl K, Marsh L, Hoffmann J, Mutgan A, Baum O, Bloch W, et al. Basement Membrane Remodeling Controls Endothelial Function in Idiopathic Pulmonary Arterial Hypertension. Am J Respir Cell Mol Biol. 2020;63:104-117 pubmed publisher
Ji Q, Qiao X, Liu Y, Wang D, Yan J. Silencing of long‑chain non‑coding RNA GAS5 in osteoarthritic chondrocytes is mediated by targeting the miR‑34a/Bcl‑2 axis. Mol Med Rep. 2020;21:1310-1319 pubmed publisher
Bozzelli P, Caccavano A, Avdoshina V, Mocchetti I, Wu J, Conant K. Increased matrix metalloproteinase levels and perineuronal net proteolysis in the HIV-infected brain; relevance to altered neuronal population dynamics. Exp Neurol. 2020;323:113077 pubmed publisher
Xiao D, Bi R, Liu X, Mei J, Jiang N, Zhu S. Notch Signaling Regulates MMP-13 Expression via Runx2 in Chondrocytes. Sci Rep. 2019;9:15596 pubmed publisher
Buken C, Sahana J, Corydon T, Melnik D, Bauer J, Wehland M, et al. Morphological and Molecular Changes in Juvenile Normal Human Fibroblasts Exposed to Simulated Microgravity. Sci Rep. 2019;9:11882 pubmed publisher
Santamaria S, Yamamoto K, Teráz Orosz A, Koch C, Apte S, de Groot R, et al. Exosites in Hypervariable Loops of ADAMTS Spacer Domains control Substrate Recognition and Proteolysis. Sci Rep. 2019;9:10914 pubmed publisher
Cao X, Luo P, Huang J, Liang C, He J, Wang Z, et al. Intraarticular senescent chondrocytes impair the cartilage regeneration capacity of mesenchymal stem cells. Stem Cell Res Ther. 2019;10:86 pubmed publisher
Lee G, Yeh C, Wu J, Lin H, Wang Y, Kuo Y, et al. TLR2 Promotes Vascular Smooth Muscle Cell Chondrogenic Differentiation and Consequent Calcification via the Concerted Actions of Osteoprotegerin Suppression and IL-6-Mediated RANKL Induction. Arterioscler Thromb Vasc Biol. 2019;39:432-445 pubmed publisher
Schulz A, Brendler J, Blaschuk O, Landgraf K, Krueger M, Ricken A. Non-pathological Chondrogenic Features of Valve Interstitial Cells in Normal Adult Zebrafish. J Histochem Cytochem. 2019;67:361-373 pubmed publisher
Zhang L, Chen Q, Wang H, Yang J, Sheng S. Andrographolide mitigates IL‑1β‑induced human nucleus pulposus cells degeneration through the TLR4/MyD88/NF‑κB signaling pathway. Mol Med Rep. 2018;18:5427-5436 pubmed publisher
Li X, Ma R, Gu Q, Liang L, Wang L, Zhang Y, et al. A fully defined static suspension culture system for large-scale human embryonic stem cell production. Cell Death Dis. 2018;9:892 pubmed publisher
Mével E, Merceron C, Vinatier C, Krisa S, Richard T, Masson M, et al. Olive and grape seed extract prevents post-traumatic osteoarthritis damages and exhibits in vitro anti IL-1? activities before and after oral consumption. Sci Rep. 2016;6:33527 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Aggrecan Monoclonal Antibody (BC-3)
Catalog # :
MA3-16888
Quantity :
100 µg
Price :
US 420.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Bovine, Canine, Equine, Feline, Guinea Pig, Human, Ovine, Porcine, Rabbit, Rat
Applications :
ELISA: 1:1,000-1:5,000, Immunocytochemistry: 2 µg/mL, Immunohistochemistry (Frozen): 1:10-1:2,000, Immunohistochemistry (Paraffin): 1:10-1:2,000, Western Blot: 1:500-1:1,000
Species :
Bovine, Canine, Equine, Feline, Guinea Pig, Human, Ovine, Porcine, Rabbit, Rat
Clone :
BC-3
Isotype :
IgG1, kappa
Storage :
-20°C
Description :
Aggrecan also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1 is a protein that is encoded by the ACAN gene. The encoded protein is an integral part of the extracellular matrix in cartilaginous tissue and it withstands compression in cartilage. The human form of the protein is 2316 amino acids long (approximately 250 kDa) and can be expressed in multiple isoforms due to alternative splicing. Aggrecan exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. It contains three interglobular domains (IGDs), G1, G2, and G3 that are involved in aggregation, hyaluronan binding, cell adhesion, and chondrocyte apoptosis. Aggrecan fragments from articular cartilage are released into the synovial fluid at all stages of human osteoarthritis as a result of cleavage by aggrecanase and MMP activity. Antibodies BC-3 and BC-4 can be used to detect these neo epitopes of Aggrecanase (ARGSVXXX) and MMP (XXXVDIPEN), respectively.
Immunogen :
Synthetic peptide corresponding to human aggrecan ARGSV neoepitope.
Format :
Liquid
Applications w/Dilutions :
ELISA: 1:1,000-1:5,000, Immunocytochemistry: 2 µg/mL, Immunohistochemistry (Frozen): 1:10-1:2,000, Immunohistochemistry (Paraffin): 1:10-1:2,000, Western Blot: 1:500-1:1,000
Aliases :
ACAN; Agc; Agc1; AGCAN; aggrecan; aggrecan 1; aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122); aggrecan core protein; Aggrecan core protein 2; aggrecan CS2 domain; aggrecan epidermal growth factor-like domain-1; aggrecan G2 domain; aggrecan interglobular domain; aggrecan keratan sulfate domain; aggrecan, structural proteoglycan of cartilage; articular cartilage aggrecan precursor; b2b183Clo; cartilage aggregating proteoglycan; cartilage-specific proteoglycan core protein; Chondroitin sulfate proteoglycan 1; chondroitin sulfate proteoglycan core protein 1; cmd; CSPCP; CSPG1; CSPGCP; high density cartilage proteoglycan; large aggregating cartilage proteoglycan core protein; large aggregating proteoglycan; large aggregating proteoglycan, antigen; MSK16; SEDK
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA