alpha Internexin Monoclonal Antibody (2E3) | Invitrogen MA3-16726 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

alpha Internexin Monoclonal Antibody (2E3)

catalog :

MA3-16726

quantity :

100 µL

price :

US 446.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2E3

reactivity :

human, mouse, rat, bovine, pigs

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; 1:800; loading ...	van der Zee J, Gijselinck I, Van Mossevelde S, Perrone F, Dillen L, Heeman B, et al. TBK1 Mutation Spectrum in an Extended European Patient Cohort with Frontotemporal Dementia and Amyotrophic Lateral Sclerosis. Hum Mutat. 2017;38:297-309 pubmed publisher
immunohistochemistry - paraffin section; human; 1:800; loading ...	Gelpi E, Hoftberger R, Graus F, Ling H, Holton J, Dawson T, et al. Neuropathological criteria of anti-IgLON5-related tauopathy. Acta Neuropathol. 2016;132:531-43 pubmed publisher
immunohistochemistry - paraffin section; human; fig 3	Inoue K, Fujimura H, Ueda K, Matsumura T, Itoh K, Sakoda S. An autopsy case of neuronal intermediate filament inclusion disease with regard to immunophenotypic and topographical analysis of the neuronal inclusions. Neuropathology. 2015;35:545-52 pubmed publisher
immunohistochemistry - paraffin section; human	Ishida M, Iwai M, Yoshida K, Kagotani A, Okabe H. Signet-ring cell melanoma with sentinel lymph node metastasis: A case report with immunohistochemical analysis and review of the clinicopathological features. Oncol Lett. 2014;7:65-68 pubmed
immunohistochemistry; human; 1:250	Shintaku M, Yoneda H, Hirato J, Nagaishi M, Okabe H. Gliosarcoma with ependymal and PNET-like differentiation. Clin Neuropathol. 2013;32:508-14 pubmed publisher
immunohistochemistry; human; 1:50	Okabe H, Nagata A, Katsura K, Ishida M, Osaka Y, Tenjin H. Cytoplasmic non-epithelial mucin accumulation associated with CD44 in an astrocytic tumor with signet ring features. Brain Tumor Pathol. 2014;31:124-30 pubmed publisher
immunohistochemistry; human	Ishida M, Shiomi H, Naka S, Tani T, Okabe H. Concomitant intraductal papillary mucinous neoplasm and neuroendocrine tumor of the pancreas. Oncol Lett. 2013;5:63-67 pubmed
	Shaw G, Morse S, Ararat M, Graham F. Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells. FASEB J. 2002;16:869-71 pubmed

image

image 1 :

Rat facial nucleus before (left) and after (right) facial nerve lesion. Note the profound upregulation of a-internexin in the facial nucleus motor neurons.

image 2 :

Hippocampal neurons in tissue culture with neurofilament alpha internexin (red), using MA3-16726 and GFAP (green).

image 3 :

Western blot of homogenate of rat facial nucleus incubated with MA3-16726, which reveals a single clean band running at approx. 66kDa.

product information

Product Type :

Antibody

Product Name :

alpha Internexin Monoclonal Antibody (2E3)

Catalog # :

MA3-16726

Quantity :

100 µL

Price :

US 446.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Bovine, Human, Mouse, Porcine, Rat

Applications :

Immunocytochemistry: 1:5,000, Immunohistochemistry (Paraffin): 1:5,000, Western Blot: 1:10,000

Species :

Bovine, Human, Mouse, Porcine, Rat

Clone :

2E3

Isotype :

IgG1

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

alpha-Internexin is a Class IV intermediate filament subunit expressed very early in neuronal development. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest.

Immunogen :

Purified recombinant rat a-internexin.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:5,000, Immunohistochemistry (Paraffin): 1:5,000, Western Blot: 1:10,000

Aliases :

66 kDa neurofilament protein; Alpha-internexin; alpha-Inx; AV028420; FLJ18662; FLJ57501; Ina; Inexa; internexin neuronal intermediate filament protein alpha; internexin neuronal intermediate filament protein, alpha; internexin, alpha; Intlaa; LOW QUALITY PROTEIN: alpha-internexin; MGC12702; NEF5; Neuro; Neurofilament 5; neurofilament 5 (66kD); Neurofilament 66; neurofilament-66; neurofilament-66, tax-binding protein; Nf66; NF-66; TXBP-1

more info or order :

Invitrogen product webpage