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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
MGMT Monoclonal Antibody (MT 23.2)
catalog :
MA3-16537
quantity :
100 µL
price :
US 456.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MT 23.2
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 12
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human; fig 3
Munthe S, Petterson S, Dahlrot R, Poulsen F, Hansen S, Kristensen B. Glioma Cells in the Tumor Periphery Have a Stem Cell Phenotype. PLoS ONE. 2016;11:e0155106 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig 4
Jakacki R, Cohen K, Buxton A, Krailo M, Burger P, Rosenblum M, et al. Phase 2 study of concurrent radiotherapy and temozolomide followed by temozolomide and lomustine in the treatment of children with high-grade glioma: a report of the Children's Oncology Group ACNS0423 study. Neuro Oncol. 2016;18:1442-50 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig 1
Bengtsson D, Schrøder H, Andersen M, Maiter D, Berinder K, Feldt Rasmussen U, et al. Long-term outcome and MGMT as a predictive marker in 24 patients with atypical pituitary adenomas and pituitary carcinomas given treatment with temozolomide. J Clin Endocrinol Metab. 2015;100:1689-98 pubmed publisher
  • immunocytochemistry; human; 1:50
  • western blot; human; 1:50
Castro G, Cayado Gutiérrez N, Zoppino F, Fanelli M, Cuello Carrión F, Sottile M, et al. Effects of temozolomide (TMZ) on the expression and interaction of heat shock proteins (HSPs) and DNA repair proteins in human malignant glioma cells. Cell Stress Chaperones. 2015;20:253-65 pubmed publisher
  • immunohistochemistry; human
Jakob J, Hille M, Sauer C, Strobel P, Wenz F, Hohenberger P. O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation is a rare event in soft tissue sarcoma. Radiat Oncol. 2012;7:180 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50; fig 4
Castro G, Cayado Gutiérrez N, Moncalero V, Lima P, De Angelis R, Chavez V, et al. Hsp27 (HSPB1): a possible surrogate molecular marker for loss of heterozygosity (LOH) of chromosome 1p in oligodendrogliomas but not in astrocytomas. Cell Stress Chaperones. 2012;17:779-90 pubmed publisher
  • immunohistochemistry; human
McCormack A, Kaplan W, Gill A, Little N, Cook R, Robinson B, et al. MGMT expression and pituitary tumours: relationship to tumour biology. Pituitary. 2013;16:208-19 pubmed publisher
  • flow cytometry; human; tbl 1
  • immunohistochemistry; human
Brell M, Ibanez J, Tortosa A. O6-Methylguanine-DNA methyltransferase protein expression by immunohistochemistry in brain and non-brain systemic tumours: systematic review and meta-analysis of correlation with methylation-specific polymerase chain reaction. BMC Cancer. 2011;11:35 pubmed publisher
  • immunohistochemistry; human
Losa M, Mazza E, Terreni M, McCormack A, Gill A, Motta M, et al. Salvage therapy with temozolomide in patients with aggressive or metastatic pituitary adenomas: experience in six cases. Eur J Endocrinol. 2010;163:843-51 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig 6
Horbinski C, Hamilton R, Lovell C, Burnham J, Pollack I. Impact of morphology, MIB-1, p53 and MGMT on outcome in pilocytic astrocytomas. Brain Pathol. 2010;20:581-8 pubmed publisher
  • immunohistochemistry; human
McCormack A, McDonald K, Gill A, Clark S, Burt M, Campbell K, et al. Low O6-methylguanine-DNA methyltransferase (MGMT) expression and response to temozolomide in aggressive pituitary tumours. Clin Endocrinol (Oxf). 2009;71:226-33 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 3
Cooper W, Kohonen Corish M, Chan C, Kwun S, McCaughan B, Kennedy C, et al. Prognostic significance of DNA repair proteins MLH1, MSH2 and MGMT expression in non-small-cell lung cancer and precursor lesions. Histopathology. 2008;52:613-22 pubmed publisher
image
image 1 :
Invitrogen MA3-16537 image 1
Detection of MGMT (Green) in Hela cells using MA3-16537 at a 1:50 dilution. Nuclei (Blue) are counterstained using Hoechst 33258.
image 2 :
Invitrogen MA3-16537 image 2
Western Blot detection of MGMT in CEM whole cell extract using MA3-16537 at 1:100 dilution.
product information
Product Type :
Antibody
Product Name :
MGMT Monoclonal Antibody (MT 23.2)
Catalog # :
MA3-16537
Quantity :
100 µL
Price :
US 456.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Human, Mouse
Applications :
Flow Cytometry: 1-2 µg/mL, Immunocytochemistry: 1:500, Immunohistochemistry (Paraffin): 1:500-1:1,000, Western Blot: 1:1,000
Species :
Human, Mouse
Clone :
MT 23.2
Isotype :
IgG2b
Storage :
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Description :
O6-Methylguanine-DNA Methyltransferase (MGMT) is an important DNA repair protein involved in tumor cell resistance to the cytostatic activity of chemotherapeutic alkylating agents. This protein is also effective in protecting normal cells against the genotoxic and carcinogenic effects of DNA alkylation. The alkylating drug resistance is caused by MGMT's ability to remove DNA alkyl groups introduced in the O6 position of guanine. MGMT is expressed in highly variable amounts, depending upon the cell and tissue type, species, and cellular growth characteristics. In addition, MGMT activity varies among groups of tumors and within a particular type of tumor.
Immunogen :
Recombinant human MGMT (O6-methylguanine-DNA methyltransferase) purified from E. coli
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 1-2 µg/mL, Immunocytochemistry: 1:500, Immunohistochemistry (Paraffin): 1:500-1:1,000, Western Blot: 1:1,000
Aliases :
0-6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase; Agat; AGT; AI267024; methylated DNA protein cysteine methyltransferase; methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; MGMT; O(6)-alkylguanine-DNA alkyltransferase; O-6-alkylguanine-DNA alkyltransferase; O6-alkylguanine-DNA alkyltransferase; O-6-methylguan; O6-methylguanine-DNA methyltranferase; O-6-methylguanine-DNA methyltransferase; O6-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA