Lamin A/C Monoclonal Antibody (mab636) | Invitrogen MA3-1000 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Lamin A/C Monoclonal Antibody (mab636)

catalog :

MA3-1000

quantity :

200 µL

price :

US 395.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

mab636

reactivity :

human, bovine, pigs

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 31

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; bovine; 1:100; loading ...; fig 3a western blot; human; loading ...; fig 3c	Heo S, Driscoll T, Thorpe S, Nerurkar N, Baker B, Yang M, et al. Differentiation alters stem cell nuclear architecture, mechanics, and mechano-sensitivity. elife. 2016;5: pubmed publisher
western blot; human; fig 2	Derussy B, Boland M, Tandon R. Human Cytomegalovirus pUL93 Links Nucleocapsid Maturation and Nuclear Egress. J Virol. 2016;90:7109-7117 pubmed publisher
western blot; human; fig 9	Eisch V, Lu X, Gabriel D, Djabali K. Progerin impairs chromosome maintenance by depleting CENP-F from metaphase kinetochores in Hutchinson-Gilford progeria fibroblasts. Oncotarget. 2016;7:24700-18 pubmed publisher
western blot; human; 1:1000; fig 4	Liu R, Li S, Garcia E, Glubrecht D, Poon H, Easaw J, et al. Association between cytoplasmic CRABP2, altered retinoic acid signaling, and poor prognosis in glioblastoma. Glia. 2016;64:963-76 pubmed publisher
immunocytochemistry; human	Sörgel S, Fraedrich K, Votteler J, Thomas M, Stamminger T, Schubert U. Perinuclear localization of the HIV-1 regulatory protein Vpr is important for induction of G2-arrest. Virology. 2012;432:444-51 pubmed publisher
western blot; human; 1:200	Lee J, Zhou S, Smas C. Identification of RANBP16 and RANBP17 as novel interaction partners for the bHLH transcription factor E12. J Cell Biochem. 2010;111:195-206 pubmed publisher
western blot; human	Lindsay A, McCaffrey M. Myosin Vb localises to nucleoli and associates with the RNA polymerase I transcription complex. Cell Motil Cytoskeleton. 2009;66:1057-72 pubmed publisher
	Betlej G, Z x105 bek T, Lewi x144 ska A, B x142 oniarz D, Rzeszutek I, Wnuk M. RNA 5-methylcytosine status is associated with DNMT2/TRDMT1 nuclear localization in osteosarcoma cell lines. J Bone Oncol. 2022;36:100448 pubmed publisher
	Li D, Fu Z, Dong C, Song Y. Downregulation of circATXN7 represses non-small cell lung cancer growth by releasing miR-7-5p. Thorac Cancer. 2022;13:1597-1610 pubmed publisher
	Guo D, Daman K, Chen J, Shi M, Yan J, Matijasevic Z, et al. iMyoblasts for ex vivo and in vivo investigations of human myogenesis and disease modeling. elife. 2022;11: pubmed publisher
	Ning J, Yang R, Wang H, Cui L. HMGB1 enhances chemotherapy resistance in multiple myeloma cells by activating the nuclear factor-κB pathway. Exp Ther Med. 2021;22:705 pubmed publisher
	Bentebbal S, Meqbel B, Salter A, Allan V, Burke B, Horn H. A human infertility-associated KASH5 variant promotes mitochondrial localization. Sci Rep. 2021;11:10133 pubmed publisher
	Gallego Valle J, Gil Manso S, Pita A, Bernaldo de Quirós E, López Esteban R, Martínez Bonet M, et al. Ectopic FOXP3 Expression in Combination with TGF-β1 and IL-2 Stimulation Generates Limited Suppressive Function in Human Primary Activated Thymocytes Ex Vivo. Biomedicines. 2021;9: pubmed publisher
	Yang F, Liu Q, Chen Y, Ye H, Wang H, Zeng S. Integrative Proteomic and Phosphoproteomic Analyses of Granulosa Cells During Follicular Atresia in Porcine. Front Cell Dev Biol. 2020;8:624985 pubmed publisher
	Rannou A, Toumaniantz G, Larcher T, Leroux I, Ledevin M, Hivonnait A, et al. Human MuStem Cell Grafting into Infarcted Rat Heart Attenuates Adverse Tissue Remodeling and Preserves Cardiac Function. Mol Ther Methods Clin Dev. 2020;18:446-463 pubmed publisher
	Alghanem B, Ali R, Nehdi A, Al Zahrani H, Altolayyan A, Shaibah H, et al. Proteomics Profiling of KAIMRC1 in Comparison to MDA-MB231 and MCF-7. Int J Mol Sci. 2020;21: pubmed publisher
	Kwon J, Vankara A, Ettyreddy A, Bohning J, Gersbach C. Myogenic Progenitor Cell Lineage Specification by CRISPR/Cas9-Based Transcriptional Activators. Stem Cell Reports. 2020;14:755-769 pubmed publisher
	Hou Y, Xie W, Yu L, Camacho L, Nie C, Zhang M, et al. Surface Roughness Gradients Reveal Topography-Specific Mechanosensitive Responses in Human Mesenchymal Stem Cells. Small. 2020;16:e1905422 pubmed publisher
	Joshi R, Stadler C, Lehmann R, Lehtiö J, Tegner J, Schmidt A, et al. TcellSubC: An Atlas of the Subcellular Proteome of Human T Cells. Front Immunol. 2019;10:2708 pubmed publisher
	Liu C, Arnold R, Henriques G, Djabali K. Inhibition of JAK-STAT Signaling with Baricitinib Reduces Inflammation and Improves Cellular Homeostasis in Progeria Cells. Cells. 2019;8: pubmed publisher
	Vo T, Jain S, Burchett R, Monckton E, Godbout R. A positive feedback loop involving nuclear factor IB and calpain 1 suppresses glioblastoma cell migration. J Biol Chem. 2019;294:12638-12654 pubmed publisher
	Sakowicz A, Lisowska M, Biesiada L, Płuciennik E, Gach A, Rybak Krzyszkowska M, et al. Placental Expression of NEMO Protein in Normal Pregnancy and Preeclampsia. Dis Markers. 2019;2019:8418379 pubmed publisher
	Adam I, Dewi D, Mooiweer J, Sadik A, Mohapatra S, Berdel B, et al. Upregulation of tryptophanyl-tRNA synthethase adapts human cancer cells to nutritional stress caused by tryptophan degradation. Oncoimmunology. 2018;7:e1486353 pubmed publisher
	Xu J, Francis A, Meuser M, Mankowski M, Ptak R, Rashad A, et al. Exploring Modifications of an HIV-1 Capsid Inhibitor: Design, Synthesis, and Mechanism of Action. J Drug Des Res. 2018;5: pubmed
	Koufaris C, Nilsson R. Protein interaction and functional data indicate MTHFD2 involvement in RNA processing and translation. Cancer Metab. 2018;6:12 pubmed publisher
	Ibba G, Piu C, Uleri E, Serra C, Dolei A. Disruption by SaCas9 Endonuclease of HERV-Kenv, a Retroviral Gene with Oncogenic and Neuropathogenic Potential, Inhibits Molecules Involved in Cancer and Amyotrophic Lateral Sclerosis. Viruses. 2018;10: pubmed publisher
	Wang X, Liu H, Zhu M, Cao C, Xu Z, Tsatskis Y, et al. Mechanical stability of the cell nucleus - roles played by the cytoskeleton in nuclear deformation and strain recovery. J Cell Sci. 2018;131: pubmed publisher
	Amador Cañizares Y, Bernier A, Wilson J, Sagan S. miR-122 does not impact recognition of the HCV genome by innate sensors of RNA but rather protects the 5' end from the cellular pyrophosphatases, DOM3Z and DUSP11. Nucleic Acids Res. 2018;46:5139-5158 pubmed publisher
	Naranjo J, Zhang H, Villar D, GonzÃ¡lez P, Dopazo X, MorÃ³n Oset J, et al. Activating transcription factor 6 derepression mediates neuroprotection in Huntington disease. J Clin Invest. 2016;126:627-38 pubmed publisher
	Elbashir S, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 2001;411:494-8 pubmed
	Röber R, Gieseler R, Peters J, Weber K, Osborn M. Induction of nuclear lamins A/C in macrophages in in vitro cultures of rat bone marrow precursor cells and human blood monocytes, and in macrophages elicited in vivo by thioglycollate stimulation. Exp Cell Res. 1990;190:185-94 pubmed

image

image 1 :

Immunofluorescent analysis of Lamin A/C using Lamin A/C Monoclonal Antibody (mab636) (Product# MA3-1000 ) shows staining in Hela Cells. Lamin A/C (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Lamin A/C (Product# MA3-1000 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

Lamin A/C Monoclonal Antibody (mab636)

Catalog # :

MA3-1000

Quantity :

200 µL

Price :

US 395.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Bovine, Human, Porcine

Applications :

Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): 1:100, Western Blot: 1:100-1:2,000

Species :

Bovine, Human, Porcine

Clone :

mab636

Isotype :

IgG2b

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).

Immunogen :

Porcine lamin preparation.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): 1:100, Western Blot: 1:100-1:2,000

Aliases :

70 kDa lamin; cb948; CDCD1; CDDC; CMD1A; CMT2B1; Dhe; EMD2; FPL; FPLD; FPLD2; HGPS; I79_009616; IDC; lamin; lamin A; lamin A/C; lamin A/C L homeolog; lamin A/C-like 1; Lamin A+C mutant; Lamin AC; lamin a-c; lamin C; lamin C2; lamin-A; Lamin-A/C; LDP1; LFP; LGMD1B; LMN1; lmna; LMNA protein; lmna.L; lmna-A; LMNC; LMNL1; mutant 453W; Mutant lamin A/C; nuclear intermediate filament protein; nuclear lamin A; prelamin-A/C; PRO1; progerin mutant; Renal carcinoma antigen NY-REN-32; RP11-54H19.1; unnamed protein product; wu:fk66d12; XELAEV_18040808mg

more info or order :

Invitrogen product webpage