Importin beta-1 Monoclonal Antibody (3E9) | Invitrogen MA3-070 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Importin beta-1 Monoclonal Antibody (3E9)

catalog :

MA3-070

quantity :

100 µg

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3E9

reactivity :

human, mouse, rat, dogs, bovine, pigs

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments, RNA immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 24

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; human; 1:100 immunocytochemistry; human	Azar W, Zivkovic S, Werther G, Russo V. IGFBP-2 nuclear translocation is mediated by a functional NLS sequence and is essential for its pro-tumorigenic actions in cancer cells. Oncogene. 2014;33:578-88 pubmed publisher
immunocytochemistry; human; 1:300	Fujimura K, Sasaki A, Anderson P. Selenite targets eIF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules. Nucleic Acids Res. 2012;40:8099-110 pubmed publisher
western blot; human	Zhong Y, Wang Y, Yang H, Ballar P, Lee J, Ye Y, et al. Importin beta interacts with the endoplasmic reticulum-associated degradation machinery and promotes ubiquitination and degradation of mutant alpha1-antitrypsin. J Biol Chem. 2011;286:33921-30 pubmed publisher
western blot; human	Hurd T, Fan S, Margolis B. Localization of retinitis pigmentosa 2 to cilia is regulated by Importin beta2. J Cell Sci. 2011;124:718-26 pubmed publisher
western blot; human; 1:5000	Smith E, Cai K, Smedberg J, Ribeiro M, Rula M, Slater C, et al. Nuclear entry of activated MAPK is restricted in primary ovarian and mammary epithelial cells. PLoS ONE. 2010;5:e9295 pubmed publisher
immunocytochemistry; human	Nakrieko K, Vespa A, Mason D, Irvine T, D Souza S, Dagnino L. Modulation of integrin-linked kinase nucleo-cytoplasmic shuttling by ILKAP and CRM1. Cell Cycle. 2008;7:2157-66 pubmed
blocking or activating experiments; human	Ivanova I, Vespa A, Dagnino L. A novel mechanism of E2F1 regulation via nucleocytoplasmic shuttling: determinants of nuclear import and export. Cell Cycle. 2007;6:2186-95 pubmed
immunocytochemistry; rat	Burry R, Smith C. HuD distribution changes in response to heat shock but not neurotrophic stimulation. J Histochem Cytochem. 2006;54:1129-38 pubmed
western blot; human; 1:2000	Stochaj U, Bański P, Kodiha M, Matusiewicz N. The N-terminal domain of the mammalian nucleoporin p62 interacts with other nucleoporins of the FXFG family during interphase. Exp Cell Res. 2006;312:2490-9 pubmed
blocking or activating experiments; human; 0.5 mg/ml	Mesika A, Kiss V, Brumfeld V, Ghosh G, Reich Z. Enhanced intracellular mobility and nuclear accumulation of DNA plasmids associated with a karyophilic protein. Hum Gene Ther. 2005;16:200-8 pubmed
immunoprecipitation; rat immunocytochemistry; rat western blot; rat immunoprecipitation; mouse immunocytochemistry; mouse western blot; mouse	Perlson E, Hanz S, Ben Yaakov K, Segal Ruder Y, Seger R, Fainzilber M. Vimentin-dependent spatial translocation of an activated MAP kinase in injured nerve. Neuron. 2005;45:715-26 pubmed
	Li H, Bi Y, Li Y, Fu R, Lv W, Jiang N, et al. A potent CBP/p300-Snail interaction inhibitor suppresses tumor growth and metastasis in wild-type p53-expressing cancer. Sci Adv. 2020;6:eaaw8500 pubmed publisher
	Zhang X, Zhou Y, Yu X, Huang Q, Fang W, Li J, et al. Differential Roles of Cysteinyl Cathepsins in TGF-β Signaling and Tissue Fibrosis. iScience. 2019;19:607-622 pubmed publisher
	Smith E, Zhang X, Capo Chichi C, Chen X, Xu X. Increased expression of Syne1/nesprin-1 facilitates nuclear envelope structure changes in embryonic stem cell differentiation. Dev Dyn. 2011;240:2245-55 pubmed publisher
	Arnold M, Nath A, Hauber J, Kehlenbach R. Multiple importins function as nuclear transport receptors for the Rev protein of human immunodeficiency virus type 1. J Biol Chem. 2006;281:20883-90 pubmed
	Hoyt C, Bouchard R, Tyler K. Novel nuclear herniations induced by nuclear localization of a viral protein. J Virol. 2004;78:6360-9 pubmed
	Rondanino C, Bousser M, Monsigny M, Roche A. Sugar-dependent nuclear import of glycosylated proteins in living cells. Glycobiology. 2003;13:509-19 pubmed
	Wamhoff B, Bowles D, Dietz N, Hu Q, Sturek M. Exercise training attenuates coronary smooth muscle phenotypic modulation and nuclear Ca2+ signaling. Am J Physiol Heart Circ Physiol. 2002;283:H2397-410 pubmed
	Brelje T, Svensson A, Stout L, Bhagroo N, Sorenson R. An immunohistochemical approach to monitor the prolactin-induced activation of the JAK2/STAT5 pathway in pancreatic islets of Langerhans. J Histochem Cytochem. 2002;50:365-83 pubmed
	Reilly J, Maher P. Importin beta-mediated nuclear import of fibroblast growth factor receptor: role in cell proliferation. J Cell Biol. 2001;152:1307-12 pubmed
	Ojala P, Sodeik B, Ebersold M, Kutay U, Helenius A. Herpes simplex virus type 1 entry into host cells: reconstitution of capsid binding and uncoating at the nuclear pore complex in vitro. Mol Cell Biol. 2000;20:4922-31 pubmed
	Takizawa C, Weis K, Morgan D. Ran-independent nuclear import of cyclin B1-Cdc2 by importin beta. Proc Natl Acad Sci U S A. 1999;96:7938-43 pubmed
	Kehlenbach R, Dickmanns A, Gerace L. Nucleocytoplasmic shuttling factors including Ran and CRM1 mediate nuclear export of NFAT In vitro. J Cell Biol. 1998;141:863-74 pubmed
	Chi N, Adam E, Adam S. Sequence and characterization of cytoplasmic nuclear protein import factor p97. J Cell Biol. 1995;130:265-74 pubmed

image

image 1 :

Immunolocalization of NTF97 in PTK cells using MA3-070

image 2 :

Immunofluorescent analysis of Importin beta-1 using anti-Importin beta-1 monoclonal antibody (Product# MA3-070) shows staining in HMVEC Cells.

product information

Product Type :

Antibody

Product Name :

Importin beta-1 Monoclonal Antibody (3E9)

Catalog # :

MA3-070

Quantity :

100 µg

Price :

US 399.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Bovine, Canine, Human, Mouse, Porcine, Rat

Applications :

Flow Cytometry: 1 µg/test, Immunocytochemistry: 1:1,000, Immunoprecipitation: Assay-dependent, Inhibition Assays: Assay-dependent, RNA Immunoprecipitation: Assay-dependent, Western Blot: 1:5,000

Species :

Bovine, Canine, Human, Mouse, Porcine, Rat

Clone :

3E9

Isotype :

IgG2a

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

Immunogen :

Purified NTF97 from bovine erythrocytes.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1 µg/test, Immunocytochemistry: 1:1,000, Immunoprecipitation: Assay-dependent, Inhibition Assays: Assay-dependent, RNA Immunoprecipitation: Assay-dependent, Western Blot: 1:5,000

Aliases :

AA409963; HGNC:6400; IMB1; Impnb; importin 1; importin 90; importin beta; importin beta-1 subunit; importin subunit beta-1; Importin-90; IPO1; IPOB; KapB1; karyopherin (importin) beta 1; karyopherin beta 1; karyopherin subunit beta 1; karyopherin subunit beta-1; karyopherin,beta 1; Kpnb1; MGC2155; MGC2156; MGC2157; NTF97; Nuclear factor p97; pore targeting complex 97 kDa subunit; PTAC97; SCG

more info or order :

Invitrogen product webpage