RAB9 Monoclonal Antibody (Mab9) | Invitrogen MA3-067 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

RAB9 Monoclonal Antibody (Mab9)

catalog :

MA3-067

quantity :

100 µL

price :

US 425.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Mab9

reactivity :

Chinese hamsters, human, rat, dogs, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Invitrogen product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:1000; fig 4	Clifford R, Maryon E, Kaplan J. Dynamic internalization and recycling of a metal ion transporter: Cu homeostasis and CTR1, the human Cuâº uptake system. J Cell Sci. 2016;129:1711-21 pubmed publisher
immunohistochemistry; human; 1:50 western blot; human; 1:500	Thoeni C, Vogel G, Tancevski I, Geley S, Lechner S, Pfaller K, et al. Microvillus inclusion disease: loss of Myosin vb disrupts intracellular traffic and cell polarity. Traffic. 2014;15:22-42 pubmed publisher
immunocytochemistry; human western blot; human	Vogt D, Camus G, Herker E, Webster B, Tsou C, Greene W, et al. Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein. PLoS Pathog. 2013;9:e1003302 pubmed publisher
immunohistochemistry; human; 1:200 western blot; human; 1:250	Uemura N, Nakanishi Y, Kato H, Nagino M, Hirohashi S, Kondo T. Antibody-based proteomics for esophageal cancer: Identification of proteins in the nuclear factor-kappaB pathway and mitotic checkpoint. Cancer Sci. 2009;100:1612-22 pubmed publisher
immunohistochemistry; human; 1:200	Cirillo C, Sarnelli G, Esposito G, Grosso M, Petruzzelli R, Izzo P, et al. Increased mucosal nitric oxide production in ulcerative colitis is mediated in part by the enteroglial-derived S100B protein. Neurogastroenterol Motil. 2009;21:1209-e112 pubmed publisher
immunohistochemistry; human; 1:200	Trere D, Brighenti E, Donati G, Ceccarelli C, Santini D, Taffurelli M, et al. High prevalence of retinoblastoma protein loss in triple-negative breast cancers and its association with a good prognosis in patients treated with adjuvant chemotherapy. Ann Oncol. 2009;20:1818-23 pubmed publisher
flow cytometry; human; 1:200	Vernet E, Lundberg E, Friedman M, Rigamonti N, Klausing S, Nygren P, et al. Affibody-mediated retention of the epidermal growth factor receptor in the secretory compartments leads to inhibition of phosphorylation in the kinase domain. N Biotechnol. 2009;25:417-23 pubmed publisher
immunocytochemistry; human; 1:30	Rögelsperger O, Ekmekcioglu C, Jager W, Klimpfinger M, Konigsberg R, Krenbek D, et al. Coexpression of the melatonin receptor 1 and nestin in human breast cancer specimens. J Pineal Res. 2009;46:422-32 pubmed publisher
western blot; human	Belov G, Habbersett C, Franco D, Ehrenfeld E. Activation of cellular Arf GTPases by poliovirus protein 3CD correlates with virus replication. J Virol. 2007;81:9259-67 pubmed
	Kane M, Diamonstein C, Hauser N, Deeken J, Niederhuber J, Vilboux T. Endosomal trafficking defects in patient cells with KIAA1109 biallelic variants. Genes Dis. 2019;6:56-67 pubmed publisher
	Lambert L, Dubayle D, Fafouri A, Herzog E, Csaba Z, Dournaud P, et al. Endocytosis of Activated Muscarinic m2 Receptor (m2R) in Live Mouse Hippocampal Neurons Occurs via a Clathrin-Dependent Pathway. Front Cell Neurosci. 2018;12:450 pubmed publisher
	Cheng X, Xie Y, Zhou B, Huang N, Farfel Becker T, Sheng Z. Characterization of LAMP1-labeled nondegradative lysosomal and endocytic compartments in neurons. J Cell Biol. 2018;217:3127-3139 pubmed publisher
	Janickova H, Prado V, Prado M, El Mestikawy S, Bernard V. Vesicular acetylcholine transporter (VAChT) over-expression induces major modifications of striatal cholinergic interneuron morphology and function. J Neurochem. 2017;142:857-875 pubmed publisher
	Zhang X, Jiang S, Mitok K, Li L, Attie A, Martin T. BAIAP3, a C2 domain-containing Munc13 protein, controls the fate of dense-core vesicles in neuroendocrine cells. J Cell Biol. 2017;216:2151-2166 pubmed publisher
	Ivanov A, Nusrat A, Parkos C. Endocytosis of epithelial apical junctional proteins by a clathrin-mediated pathway into a unique storage compartment. Mol Biol Cell. 2004;15:176-88 pubmed
	Cheng F, Mani K, van den Born J, Ding K, Belting M, Fransson L. Nitric oxide-dependent processing of heparan sulfate in recycling S-nitrosylated glypican-1 takes place in caveolin-1-containing endosomes. J Biol Chem. 2002;277:44431-9 pubmed
	Lorenzo M, Jung J, Ploegh H. Kaposi's sarcoma-associated herpesvirus K3 utilizes the ubiquitin-proteasome system in routing class major histocompatibility complexes to late endocytic compartments. J Virol. 2002;76:5522-31 pubmed
	Soldati T, Riederer M, Pfeffer S. Rab GDI: a solubilizing and recycling factor for rab9 protein. Mol Biol Cell. 1993;4:425-34 pubmed
	Shapiro A, Pfeffer S. Quantitative analysis of the interactions between prenyl Rab9, GDP dissociation inhibitor-alpha, and guanine nucleotides. J Biol Chem. 1995;270:11085-90 pubmed

image

image 1 :

Immunofluorescent analysis of RAB9 using RAB9 Monoclonal Antibody (Mab9) (Product# MA3-067 ) shows staining in A2058 Cells. RAB9 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing RAB9 (Product# MA3-067 ) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

RAB9 Monoclonal Antibody (Mab9)

Catalog # :

MA3-067

Quantity :

100 µL

Price :

US 425.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Bovine, Canine, Hamster, Human, Non-human primate, Rat

Applications :

Immunocytochemistry: 1:200-1:500, Immunohistochemistry: 1:20, Immunomicroscopy: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000-1:5,000

Species :

Bovine, Canine, Hamster, Human, Non-human primate, Rat

Clone :

Mab9

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.

Immunogen :

Recombinant canine rab 9a.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:200-1:500, Immunohistochemistry: 1:20, Immunomicroscopy: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000-1:5,000

Aliases :

2410064E05Rik; AI195561; rab9; rab9 GTP-binding protein; RAB9, member RAS oncogene family; Rab9a; rab-9A; RAB9A, member RAS oncogene family; RAB9-like protein; ras-related protein Rab-9A; Sid 99; Sid6061p; Sid99

more info or order :

Invitrogen product webpage