alpha Adaptin Monoclonal Antibody (AC1-M11) | Invitrogen MA3-061 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

alpha Adaptin Monoclonal Antibody (AC1-M11)

catalog :

MA3-061

quantity :

500 µL

price :

US 425.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AC1-M11

reactivity :

human, mouse, rat, bovine, pigs

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 26

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:500; loading ...; fig 5	Evans H, Bodea L, Götz J. Cell-specific non-canonical amino acid labelling identifies changes in the de novo proteome during memory formation. elife. 2020;9: pubmed publisher
western blot; mouse; loading ...; fig 3a	Richter K, Schmutz I, Darna M, Zander J, Chavan R, Albrecht U, et al. VGLUT1 Binding to Endophilin or Intersectin1 and Dynamin Phosphorylation in a Diurnal Context. Neuroscience. 2018;371:29-37 pubmed publisher
immunoprecipitation; human; fig s1a western blot; human; fig s1a	Kadlecova Z, Spielman S, Loerke D, Mohanakrishnan A, Reed D, Schmid S. Regulation of clathrin-mediated endocytosis by hierarchical allosteric activation of AP2. J Cell Biol. 2017;216:167-179 pubmed publisher
immunocytochemistry; human; loading ...; fig 5a western blot; human; loading ...; fig 4b	Elkin S, Oswald N, Reed D, Mettlen M, Macmillan J, Schmid S. Ikarugamycin: A Natural Product Inhibitor of Clathrin-Mediated Endocytosis. Traffic. 2016;17:1139-49 pubmed publisher
western blot; rat; 1:1000	Rong Y, Liu M, Ma L, Du W, Zhang H, Tian Y, et al. Clathrin and phosphatidylinositol-4,5-bisphosphate regulate autophagic lysosome reformation. Nat Cell Biol. 2012;14:924-34 pubmed publisher
western blot; mouse	Milosevic I, Giovedi S, Lou X, Raimondi A, Collesi C, Shen H, et al. Recruitment of endophilin to clathrin-coated pit necks is required for efficient vesicle uncoating after fission. Neuron. 2011;72:587-601 pubmed publisher
western blot; rat	Zander J, Münster Wandowski A, Brunk I, Pahner I, Gómez Lira G, Heinemann U, et al. Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses. J Neurosci. 2010;30:7634-45 pubmed publisher
western blot; rat	Marin M, Esteban Pretel G, Ponsoda X, Romero A, Ballestín R, Lopez C, et al. Endocytosis in cultured neurons is altered by chronic alcohol exposure. Toxicol Sci. 2010;115:202-13 pubmed publisher
western blot; mouse	Matsuda N, Yamamoto S, Takano K, Kageyama S, Kurobe Y, Yoshihara Y, et al. Silencing of fas-associated death domain protects mice from septic lung inflammation and apoptosis. Am J Respir Crit Care Med. 2009;179:806-15 pubmed publisher
immunocytochemistry; human	Yoon H, Lee J, Randazzo P. ARAP1 regulates endocytosis of EGFR. Traffic. 2008;9:2236-52 pubmed publisher
immunocytochemistry; mouse	Ferguson S, Brasnjo G, Hayashi M, Wölfel M, Collesi C, Giovedi S, et al. A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. Science. 2007;316:570-4 pubmed
immunohistochemistry; human	Kim S, Choi H, Lee K, Hong N, Sung B, Choi K, et al. Interaction of SPIN90 with syndapin is implicated in clathrin-mediated endocytic pathway in fibroblasts. Genes Cells. 2006;11:1197-211 pubmed
immunoprecipitation; human	Austin C, Lawrence D, Peden A, Varfolomeev E, Totpal K, De MaziÃ¨re A, et al. Death-receptor activation halts clathrin-dependent endocytosis. Proc Natl Acad Sci U S A. 2006;103:10283-10288 pubmed publisher
western blot; rat; loading ...; fig 3a	Krauss M, Haucke V. Functional assay of effectors of ADP ribosylation factor 6 during clathrin/AP-2 coat recruitment to membranes. Methods Enzymol. 2005;404:388-98 pubmed
western blot; mouse; loading ...; fig 2c	Shimomura J, Ishibashi O, Ikegame M, Yoshizawa T, Ejiri S, Noda T, et al. Tensile stress induces alpha-adaptin C production in mouse calvariae in an organ culture: possible involvement of endocytosis in mechanical stress-stimulated osteoblast differentiation. J Cell Physiol. 2003;195:488-96 pubmed
	Mandarano M, Bellezza G, Belladonna M, Vannucci J, Gili A, Ferri I, et al. Indoleamine 2,3-Dioxygenase 2 Immunohistochemical Expression in Resected Human Non-small Cell Lung Cancer: A Potential New Prognostic Tool. Front Immunol. 2020;11:839 pubmed publisher
	Wang X, Chen Z, Mettlen M, Noh J, Schmid S, Danuser G. DASC, a sensitive classifier for measuring discrete early stages in clathrin-mediated endocytosis. elife. 2020;9: pubmed publisher
	Wolfram J, Nizzero S, Liu H, Li F, Zhang G, Li Z, et al. A chloroquine-induced macrophage-preconditioning strategy for improved nanodelivery. Sci Rep. 2017;7:13738 pubmed publisher
	Ivanov A, Nusrat A, Parkos C. Endocytosis of epithelial apical junctional proteins by a clathrin-mediated pathway into a unique storage compartment. Mol Biol Cell. 2004;15:176-88 pubmed
	Zhou Y, Zhang J, King M. Xenopus autosomal recessive hypercholesterolemia protein couples lipoprotein receptors with the AP-2 complex in oocytes and embryos and is required for vitellogenesis. J Biol Chem. 2003;278:44584-92 pubmed
	Bild A, Turkson J, Jove R. Cytoplasmic transport of Stat3 by receptor-mediated endocytosis. EMBO J. 2002;21:3255-63 pubmed
	Engqvist Goldstein A, Warren R, Kessels M, Keen J, Heuser J, Drubin D. The actin-binding protein Hip1R associates with clathrin during early stages of endocytosis and promotes clathrin assembly in vitro. J Cell Biol. 2001;154:1209-23 pubmed
	Vleurick L, Pezet A, Kuhn E, Decuypere E, Edery M. A beta-turn endocytic code is required for optimal internalization of the growth hormone receptor but not for alpha-adaptin association. Mol Endocrinol. 1999;13:1823-31 pubmed
	Tebar F, Bohlander S, Sorkin A. Clathrin assembly lymphoid myeloid leukemia (CALM) protein: localization in endocytic-coated pits, interactions with clathrin, and the impact of overexpression on clathrin-mediated traffic. Mol Biol Cell. 1999;10:2687-702 pubmed
	Chang M, Mallet W, Mostov K, Brodsky F. Adaptor self-aggregation, adaptor-receptor recognition and binding of alpha-adaptin subunits to the plasma membrane contribute to recruitment of adaptor (AP2) components of clathrin-coated pits. EMBO J. 1993;12:2169-80 pubmed
	Wong D, Brodsky F. 100-kD proteins of Golgi- and trans-Golgi network-associated coated vesicles have related but distinct membrane binding properties. J Cell Biol. 1992;117:1171-9 pubmed

product information

Product Type :

Antibody

Product Name :

alpha Adaptin Monoclonal Antibody (AC1-M11)

Catalog # :

MA3-061

Quantity :

500 µL

Price :

US 425.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Amphibian, Bovine, Chicken, Human, Mouse, Non-human primate, Porcine, Rat

Applications :

Immunocytochemistry: 1:100, Immunohistochemistry (Paraffin): 1:20, Immunoprecipitation: Assay-dependent, Western Blot: 2 µg/mL

Species :

Amphibian, Bovine, Chicken, Human, Mouse, Non-human primate, Porcine, Rat

Clone :

AC1-M11

Isotype :

IgG2a

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.

Immunogen :

Purified bovine brain adaptor complexes.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:100, Immunohistochemistry (Paraffin): 1:20, Immunoprecipitation: Assay-dependent, Western Blot: 2 µg/mL

Aliases :

100 kDa coated vesicle protein A; adapter-related protein complex 2 alpha-1 subunit; adapter-related protein complex 2 subunit alpha-1; adaptin, alpha A; adaptor protein complex AP-2 subunit alpha-1; adaptor protein complex AP-2, alpha 1 subunit; adaptor related protein complex 2 alpha 1 subunit; adaptor related protein complex 2 subunit alpha 1; adaptor related protein complex 2, alpha 1 subunit; adaptor-related protein complex 2 subunit alpha-1; adaptor-related protein complex 2, alpha 1 subunit; adaptor-related protein complex 2, short form; adaptor-related protein complex AP-2, alpha 1 subunit; Adtaa; ADTAB; alpha1-adaptin; alpha2-adaptin; Alpha-adaptin A; alpha-adaptin C; AP2; AP-2 complex subunit alpha-1; Ap2a1; AP2-ALPHA; CLAPA1; CLAPA2; Clathrin assembly protein complex 2 alpha-A large chain; clathrin-associated/assembly/adaptor protein, large, alpha 1; HIP9; HIP-9; HYPJ; plasma membrane adaptor HA2/AP2 adaptin alpha A subunit

more info or order :

Invitrogen product webpage