ARF1/ARF3/ARF5/ARF6 Monoclonal Antibody (1D9) | Invitrogen MA3-060 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

ARF1/ARF3/ARF5/ARF6 Monoclonal Antibody (1D9)

catalog :

MA3-060

quantity :

100 µL

price :

US 425.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1D9

reactivity :

Chinese hamsters, human, mouse, rat, dogs

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Invitrogen product webpage

citations: 21

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; fig 9 western blot; human; fig 3	Inoue H, Ha V, Prekeris R, Randazzo P. Arf GTPase-activating protein ASAP1 interacts with Rab11 effector FIP3 and regulates pericentrosomal localization of transferrin receptor-positive recycling endosome. Mol Biol Cell. 2008;19:4224-37 pubmed publisher
western blot; human; 1:100	Kumari S, Mayor S. ARF1 is directly involved in dynamin-independent endocytosis. Nat Cell Biol. 2008;10:30-41 pubmed
western blot; human	Belov G, Habbersett C, Franco D, Ehrenfeld E. Activation of cellular Arf GTPases by poliovirus protein 3CD correlates with virus replication. J Virol. 2007;81:9259-67 pubmed
western blot; human	Shen X, Xu K, Fan Q, Pacheco Rodriguez G, Moss J, Vaughan M. Association of brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2) with recycling endosomes during transferrin uptake. Proc Natl Acad Sci U S A. 2006;103:2635-40 pubmed
western blot; human	Choi W, Karim Z, Whiteheart S. Arf6 plays an early role in platelet activation by collagen and convulxin. Blood. 2006;107:3145-52 pubmed
western blot; human	Belov G, Fogg M, Ehrenfeld E. Poliovirus proteins induce membrane association of GTPase ADP-ribosylation factor. J Virol. 2005;79:7207-16 pubmed
immunocytochemistry; human; fig 5 western blot; human; fig 8	Dell Angelica E, Puertollano R, Mullins C, Aguilar R, Vargas J, Hartnell L, et al. GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex. J Cell Biol. 2000;149:81-94 pubmed
	Yamanaka T, Tosaki A, Kurosawa M, Matsumoto G, Koike M, Uchiyama Y, et al. NF-Y inactivation causes atypical neurodegeneration characterized by ubiquitin and p62 accumulation and endoplasmic reticulum disorganization. Nat Commun. 2014;5:3354 pubmed publisher
	Wang J, Sun H, Macia E, Kirchhausen T, Watson H, Bonifacino J, et al. PI4P promotes the recruitment of the GGA adaptor proteins to the trans-Golgi network and regulates their recognition of the ubiquitin sorting signal. Mol Biol Cell. 2007;18:2646-55 pubmed
	Hashimoto S, Onodera Y, Hashimoto A, Tanaka M, Hamaguchi M, Yamada A, et al. Requirement for Arf6 in breast cancer invasive activities. Proc Natl Acad Sci U S A. 2004;101:6647-52 pubmed
	Chan D, Strang M, Judson B, Brown W. Inhibition of membrane tubule formation and trafficking by isotetrandrine, an antagonist of G-protein-regulated phospholipase A2 enzymes. Mol Biol Cell. 2004;15:1871-80 pubmed
	Spitzenberger F, Pietropaolo S, Verkade P, Habermann B, Lacas Gervais S, Mziaut H, et al. Islet cell autoantigen of 69 kDa is an arfaptin-related protein associated with the Golgi complex of insulinoma INS-1 cells. J Biol Chem. 2003;278:26166-73 pubmed
	Pullikuth A, Weidman P. In vitro transport on cis and trans sides of the Golgi involves two distinct types of coatomer and ADP-ribosylation factor-independent transport intermediates. J Biol Chem. 2002;277:50355-64 pubmed
	Lu L, Horstmann H, Ng C, Hong W. Regulation of Golgi structure and function by ARF-like protein 1 (Arl1). J Cell Sci. 2001;114:4543-55 pubmed
	Ahluwalia J, Topp J, Weirather K, Zimmerman M, Stamnes M. A role for calcium in stabilizing transport vesicle coats. J Biol Chem. 2001;276:34148-55 pubmed
	Mazaki Y, Hashimoto S, Okawa K, Tsubouchi A, Nakamura K, Yagi R, et al. An ADP-ribosylation factor GTPase-activating protein Git2-short/KIAA0148 is involved in subcellular localization of paxillin and actin cytoskeletal organization. Mol Biol Cell. 2001;12:645-62 pubmed
	Ooi C, Dell Angelica E, Bonifacino J. ADP-Ribosylation factor 1 (ARF1) regulates recruitment of the AP-3 adaptor complex to membranes. J Cell Biol. 1998;142:391-402 pubmed
	Happe S, Weidman P. Cell-free transport to distinct Golgi cisternae is compartment specific and ARF independent. J Cell Biol. 1998;140:511-23 pubmed
	Cavenagh M, Whitney J, Carroll K, Zhang C, Boman A, Rosenwald A, et al. Intracellular distribution of Arf proteins in mammalian cells. Arf6 is uniquely localized to the plasma membrane. J Biol Chem. 1996;271:21767-74 pubmed
	Serafini T, Orci L, Amherdt M, Brunner M, Kahn R, Rothman J. ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding protein. Cell. 1991;67:239-53 pubmed
	Zeuzem S, Feick P, Zimmermann P, Haase W, Kahn R, Schulz I. Intravesicular acidification correlates with binding of ADP-ribosylation factor to microsomal membranes. Proc Natl Acad Sci U S A. 1992;89:6619-23 pubmed

image

image 1 :

Immunofluorescent analysis of ADP-Ribosylation Factor using ADP-Ribosylation Factor Monoclonal Antibody (1D9) (Product# MA3-060 ) shows staining in MCF-7 Cells. ADP-Ribosylation Factor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing ADP-Ribosylation Factor (Product# MA3-060 ) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

ARF1/ARF3/ARF5/ARF6 Monoclonal Antibody (1D9)

Catalog # :

MA3-060

Quantity :

100 µL

Price :

US 425.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Canine, Hamster, Human, Mouse, Rat

Applications :

Flow Cytometry: 1/20, Immunocytochemistry: 1:100, Immunohistochemistry: 1:100, Immunoprecipitation: Assay-dependent, Western Blot: 1:3000

Species :

Canine, Hamster, Human, Mouse, Rat

Clone :

1D9

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Immunogen :

Recombinant human ARF1.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1/20, Immunocytochemistry: 1:100, Immunohistochemistry: 1:100, Immunoprecipitation: Assay-dependent, Western Blot: 1:3000

Aliases :

5430400P17Rik; AA407803; Ac1-253; ADP ribosylation factor 1; ADP ribosylation factor 3; ADP ribosylation factor 4; ADP ribosylation factor 5; ADP ribosylation factor 6; ADP-ribosylation factor 1; ADP-ribosylation factor 2; ADP-ribosylation factor 3; ADP-ribosylation factor 4; ADP-ribosylation factor 4-like protein; ADP-ribosylation factor 5; ADP-ribosylation factor 6; AI788669; AI854770; ARF; Arf1; Arf2; ARF3; ARF4; Arf5; ARF6; AW496366; I79_003112; Liver regeneration-related protein LRRG202; pan ARF; small GTP binding protein

more info or order :

Invitrogen product webpage