CHRNA1 Monoclonal Antibody (88B) | Invitrogen MA3-043 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CHRNA1 Monoclonal Antibody (88B)

catalog :

MA3-043

quantity :

100 µL

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

88B

reactivity :

human, mouse, rat, chicken

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 4d	Xing G, Jing H, Zhang L, Cao Y, Li L, Zhao K, et al. A mechanism in agrin signaling revealed by a prevalent Rapsyn mutation in congenital myasthenic syndrome. elife. 2019;8: pubmed publisher
western blot; rat	Mulvey C, Harno E, Keenan A, Ohlendieck K. Expression of the skeletal muscle dystrophin-dystroglycan complex and syntrophin-nitric oxide synthase complex is severely affected in the type 2 diabetic Goto-Kakizaki rat. Eur J Cell Biol. 2005;84:867-83 pubmed
	Mulvey C, Ohlendieck K. Use of continuous-elution gel electrophoresis as a preparative tool for blot overlay analysis. Anal Biochem. 2003;319:122-30 pubmed
	O Reilly C, Pette D, Ohlendieck K. Increased expression of the nicotinic acetylcholine receptor in stimulated muscle. Biochem Biophys Res Commun. 2003;300:585-91 pubmed
	Sala C, O Malley J, Xu R, Fumagalli G, Salpeter M. Epsilon subunit-containing acetylcholine receptors in myotubes belong to the slowly degrading population. J Neurosci. 1997;17:8937-44 pubmed
	Dmytrenko G, Pumplin D, Bloch R. Dystrophin in a membrane skeletal network: localization and comparison to other proteins. J Neurosci. 1993;13:547-58 pubmed
	Bloch R, Sealock R, Pumplin D, Luther P, Froehner S. Association of acetylcholine receptors with peripheral membrane proteins: evidence from antibody-induced coaggregation. J Membr Biol. 1994;138:13-28 pubmed
	Froehner S, Douville K, Klink S, Culp W. Monoclonal antibodies to cytoplasmic domains of the acetylcholine receptor. J Biol Chem. 1983;258:7112-20 pubmed
	Morton M, Froehner S. Monoclonal antibody identifies a 200-kDa subunit of the dihydropyridine-sensitive calcium channel. J Biol Chem. 1987;262:11904-7 pubmed

image

image 1 :

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse kidney tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Nicotinic Acetylcholine Receptor (MA3-043) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

image 2 :

Immunofluorescent analysis of Nicotinic Acetylcholine Receptor using Anti-Nicotinic Acetylcholine Receptor Monoclonal Antibody (88B) (Product# MA3-043) shows staining in Neuro-2a Cells. Nicotinic Acetylcholine Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Nicotinic Acetylcholine Receptor (Product# MA3-043) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

CHRNA1 Monoclonal Antibody (88B)

Catalog # :

MA3-043

Quantity :

100 µL

Price :

US 399.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Amphibian, Chicken, Fish, Human, Mouse, Rat

Applications :

Immunocytochemistry: 1:20-100, Immunohistochemistry (Frozen): 1:1,000, Immunoprecipitation: Assay-dependent, Western Blot: 1:5,000

Species :

Amphibian, Chicken, Fish, Human, Mouse, Rat

Clone :

88B

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine.The nAChRs fall into two subclasses, the muscle-type and the neuronal-type. The muscle-type receptor is a pentameric glycoprotein of five membrane spanning subunits, (2 alpha 1's, beta 1, gamma or epsilon and delta) which form a ligand gated ion channel. The neuronal nAChR is also a pentamer, but unlike the muscle-type, is made up of only two general types of subunits, alpha and beta of which multiple subtypes have been described (alpha 2 to alpha 8 and beta 2 to beta 4).

Immunogen :

Purified Torpedo californica acetylcholine receptor.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:20-100, Immunohistochemistry (Frozen): 1:1,000, Immunoprecipitation: Assay-dependent, Western Blot: 1:5,000

Aliases :

acetylcholine receptor; Acetylcholine receptor subunit alpha; acetylcholine receptor, nicotinic, alpha 1 (muscle); Achr-1; ACHRA; ACHRD; Acra; AI385656; AI608266; alpha-1 subunit, nicotinic acetylcholine receptor; CHNRA; cholinergic receptor; cholinergic receptor nicotinic alpha 1 subunit; cholinergic receptor nicotinic alpha polypeptide 1; cholinergic receptor, nicotinic alpha 1; cholinergic receptor, nicotinic, alpha 1 (muscle); cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle); CHRNA; CHRNA1; CMS1A; CMS1B; CMS2A; FCCMS; muscle nicotinic acetylcholine receptor; nAChR; NACHRA1; nicotinic acetylcholine receptor alpha subunit; nicotinic acetylcholine receptor, alpha-1 subunit; nicotinic cholinergic receptor alpha 1; SCCMS

more info or order :

Invitrogen product webpage