Acetylcholinesterase Monoclonal Antibody (HR2) | Invitrogen MA3-042 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Acetylcholinesterase Monoclonal Antibody (HR2)

catalog :

MA3-042

quantity :

200 µL

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HR2

reactivity :

African green monkey, guinea pig, human, mouse, bovine, cat, domestic rabbit

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
ELISA; human	Darreh Shori T, Vijayaraghavan S, Aeinehband S, Piehl F, Lindblom R, Nilsson B, et al. Functional variability in butyrylcholinesterase activity regulates intrathecal cytokine and astroglial biomarker profiles in patients with Alzheimer's disease. Neurobiol Aging. 2013;34:2465-81 pubmed publisher
immunohistochemistry; human	Hayashi M, Ohto T, Shioda K, Fukatsu R. Lesions of cortical GABAergic interneurons and acetylcholine neurons in xeroderma pigmentosum group A. Brain Dev. 2012;34:287-92 pubmed publisher
immunohistochemistry; human; 1:250	Hayashi M, Miyata R, Tanuma N. Decrease in acetylcholinergic neurons in the pedunculopontine tegmental nucleus in a patient with Prader-Willi syndrome. Neuropathology. 2011;31:280-5 pubmed publisher
immunoprecipitation; human	García Ayllón M, Riba Llena I, Serra Basante C, Alom J, Boopathy R, Saez Valero J. Altered levels of acetylcholinesterase in Alzheimer plasma. PLoS ONE. 2010;5:e8701 pubmed publisher
western blot; human; 1:1000	Nordberg A, Darreh Shori T, Peskind E, Soininen H, Mousavi M, Eagle G, et al. Different cholinesterase inhibitor effects on CSF cholinesterases in Alzheimer patients. Curr Alzheimer Res. 2009;6:4-14 pubmed
immunoprecipitation; human immunocytochemistry; human; 1:200 western blot; human; 1:200 immunocytochemistry; African green monkey	Silveyra M, Evin G, Montenegro M, Vidal C, Martinez S, Culvenor J, et al. Presenilin 1 interacts with acetylcholinesterase and alters its enzymatic activity and glycosylation. Mol Cell Biol. 2008;28:2908-19 pubmed publisher
immunoprecipitation; human ELISA; human; 1:1000	Darreh Shori T, Kadir A, Almkvist O, Grut M, Wall A, Blomquist G, et al. Inhibition of acetylcholinesterase in CSF versus brain assessed by 11C-PMP PET in AD patients treated with galantamine. Neurobiol Aging. 2008;29:168-84 pubmed
immunoprecipitation; human ELISA; human; 1:1000 western blot; human	Darreh Shori T, Meurling L, Pettersson T, Hugosson K, Hellström Lindahl E, Andreasen N, et al. Changes in the activity and protein levels of CSF acetylcholinesterases in relation to cognitive function of patients with mild Alzheimer's disease following chronic donepezil treatment. J Neural Transm (Vienna). 2006;113:1791-801 pubmed
immunohistochemistry; human; 1:30000	McDonald T, Li C, Vincent S, Nijland M. Fetal fornix transection and gestation length in sheep. Exp Neurol. 2006;200:532-7 pubmed
	Alvarez Carbonell D, Ye F, Ramanath N, Garcia Mesa Y, Knapp P, Hauser K, et al. Cross-talk between microglia and neurons regulates HIV latency. PLoS Pathog. 2019;15:e1008249 pubmed publisher
	Gu W, Ni Z, Tan Y, Deng J, Zhang S, Lv Z, et al. Adventitial Cell Atlas of wt (Wild Type) and ApoE (Apolipoprotein E)-Deficient Mice Defined by Single-Cell RNA Sequencing. Arterioscler Thromb Vasc Biol. 2019;39:1055-1071 pubmed publisher
	Napon G, Dafferner A, Saxena A, Lockridge O. Identification of Carboxylesterase, Butyrylcholinesterase, Acetylcholinesterase, Paraoxonase, and Albumin Pseudoesterase in Guinea Pig Plasma through Nondenaturing Gel Electrophoresis. Comp Med. 2018;68:367-374 pubmed publisher
	Dafferner A, Schopfer L, Xiao G, Cashman J, Yerramalla U, Johnson R, et al. Immunopurification of Acetylcholinesterase from Red Blood Cells for Detection of Nerve Agent Exposure. Chem Res Toxicol. 2017;30:1897-1910 pubmed publisher
	Rakonczay Z, Brimijoin S. Monoclonal antibodies to human brain acetylcholinesterase: properties and applications. Cell Mol Neurobiol. 1988;8:85-93 pubmed
	Mesulam M, Geula C, Cosgrove R, Mash D, Brimijoin S. Immunocytochemical demonstration of axonal and perikaryal acetylcholinesterase in human cerebral cortex. Brain Res. 1991;539:233-8 pubmed

image

image 1 :

Immunofluorescent analysis of Acetylcholinesterase using Anti-Acetylcholinesterase Monoclonal Antibody (HR2) (Product# MA3-042) shows staining in Hela Cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase (Product# MA3-042) at a dilution of 1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

Acetylcholinesterase Monoclonal Antibody (HR2)

Catalog # :

MA3-042

Quantity :

200 µL

Price :

US 399.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Bovine, Feline, Guinea Pig, Human, Mouse, Rabbit

Applications :

ELISA: Assay-dependent, Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Frozen): 1:50, Immunohistochemistry (Paraffin): 1:20-1:200, Immunoprecipitation: Assay-dependent

Species :

Bovine, Feline, Guinea Pig, Human, Mouse, Rabbit

Clone :

HR2

Isotype :

IgG2b

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. The Protein is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits.

Immunogen :

Purified human cerebellar acetylcholinesterase.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Frozen): 1:50, Immunohistochemistry (Paraffin): 1:20-1:200, Immunoprecipitation: Assay-dependent

Aliases :

ACE-1; ACEE; Acetylcholinesterase; acetylcholinesterase (Cartwright blood group); acetylcholinesterase (Yt blood group); acetylcholinesterase T-subunit; ACHE; ache protein; apoptosis-related acetylcholinesterase; ARACHE; EC 3.1.1.7; glycolipid-anchored form of acetylcholinesterase; N-ACHE; OTTHUMP00000211349; OTTHUMP00000211356; YT; Yt blood group; zgc:92550; ziehharmonika; zim

more info or order :

Invitrogen product webpage