Cdc37 Monoclonal Antibody (C1) | Invitrogen MA3-029 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cdc37 Monoclonal Antibody (C1)

catalog :

MA3-029

quantity :

100 µL

price :

US 425.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

Chinese hamsters, human, mouse, rat, fruit fly , domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 6c	Lopez V, Park B, Nowak D, Sreelatha A, Zembek P, Fernandez J, et al. A Bacterial Effector Mimics a Host HSP90 Client to Undermine Immunity. Cell. 2019;179:205-218.e21 pubmed publisher
western blot; mouse; fig 1	Ono R, Kaisho T, Tanaka T. PDLIM1 inhibits NF-ÎºB-mediated inflammatory signaling by sequestering the p65 subunit of NF-ÎºB in the cytoplasm. Sci Rep. 2015;5:18327 pubmed publisher
western blot; human; fig 4	Pare J, LaPointe P, Hobman T. Hsp90 cochaperones p23 and FKBP4 physically interact with hAgo2 and activate RNA interference-mediated silencing in mammalian cells. Mol Biol Cell. 2013;24:2303-10 pubmed publisher
immunoprecipitation; mouse	Taherian A, Krone P, Ovsenek N. A comparison of Hsp90alpha and Hsp90beta interactions with cochaperones and substrates. Biochem Cell Biol. 2008;86:37-45 pubmed publisher
western blot; fruit fly	Felts S, Karnitz L, Toft D. Functioning of the Hsp90 machine in chaperoning checkpoint kinase I (Chk1) and the progesterone receptor (PR). Cell Stress Chaperones. 2007;12:353-63 pubmed
immunoprecipitation; domestic rabbit western blot; domestic rabbit western blot; human	Prince T, Shao J, Matts R, Hartson S. Evidence for chaperone heterocomplexes containing both Hsp90 and VCP. Biochem Biophys Res Commun. 2005;331:1331-7 pubmed
	Lei W, Duron D, Stine C, Mishra S, Blagg B, Streicher J. The Alpha Isoform of Heat Shock Protein 90 and the Co-chaperones p23 and Cdc37 Promote Opioid Anti-nociception in the Brain. Front Mol Neurosci. 2019;12:294 pubmed publisher
	Narasimhan S. A Commitment to Gender Diversity in Peer Review. Cell. 2019;179:1-2 pubmed publisher
	Anderson I, Low J, Weston S, Weinberger M, Zhyvoloup A, Labokha A, et al. Heat shock protein 90 controls HIV-1 reactivation from latency. Proc Natl Acad Sci U S A. 2014;111:E1528-37 pubmed publisher
	Arlander S, Felts S, Wagner J, Stensgard B, Toft D, Karnitz L. Chaperoning checkpoint kinase 1 (Chk1), an Hsp90 client, with purified chaperones. J Biol Chem. 2006;281:2989-98 pubmed
	Goetz M, Toft D, Reid J, Ames M, Stensgard B, Safgren S, et al. Phase I trial of 17-allylamino-17-demethoxygeldanamycin in patients with advanced cancer. J Clin Oncol. 2005;23:1078-87 pubmed
	Hartson S, Irwin A, Shao J, Scroggins B, Volk L, Huang W, et al. p50(cdc37) is a nonexclusive Hsp90 cohort which participates intimately in Hsp90-mediated folding of immature kinase molecules. Biochemistry. 2000;39:7631-44 pubmed
	Kimura Y, Rutherford S, Miyata Y, Yahara I, Freeman B, Yue L, et al. Cdc37 is a molecular chaperone with specific functions in signal transduction. Genes Dev. 1997;11:1775-85 pubmed
	Lamphere L, Fiore F, Xu X, Brizuela L, Keezer S, Sardet C, et al. Interaction between Cdc37 and Cdk4 in human cells. Oncogene. 1997;14:1999-2004 pubmed
	Perdew G, Wiegand H, Vanden Heuvel J, Mitchell C, Singh S. A 50 kilodalton protein associated with raf and pp60(v-src) protein kinases is a mammalian homolog of the cell cycle control protein cdc37. Biochemistry. 1997;36:3600-7 pubmed
	Milam A, De Leeuw A, Gaur V, Saari J. Immunolocalization of cellular retinoic acid binding protein to Müller cells and/or a subpopulation of GABA-positive amacrine cells in retinas of different species. J Comp Neurol. 1990;296:123-9 pubmed

image

image 1 :

Immunofluorescent analysis of Cdc37 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cdc37 monoclonal antibody (Product # MA3-029) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Cdc37 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

Cdc37 Monoclonal Antibody (C1)

Catalog # :

MA3-029

Quantity :

100 µL

Price :

US 425.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Hamster, Human, Mouse, Rat

Applications :

Flow Cytometry: 1:50, Immunocytochemistry: 1:200, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Species :

Hamster, Human, Mouse, Rat

Clone :

Isotype :

IgG2b

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

In S. cerevisiae, Cdc37 has been shown to be an important regulator of cell division cycle through its action on cyclin-dependent kinases (CDK's). Cdc37 has also been associated with signaling pathways and kinases unrelated to cell cycle control, including v-Src, casein kinase II, MPS1 kinase and the sevenless receptor tyrosine kinase. Interestingly, HSP90 has also been shown to be associated with many of the same cellular targets as Cdc37. It has now been shown that Cdc37 is the same protein previously described as p50 which has been found to be associated with many of the same proteins as HSP90. Among others, Cdc37/p50 has been found complexed with Src-family kinases (Fyn, Yes, Fes, Lck & Fgr), Raf and pp60v-src. More recently, Cdc37 has been shown to be a chaperone independently and in concert with HSP90. In fact, Cdc37 is necessary for the binding of HSP90 to Cdk4.

Immunogen :

Purified mouse Cdc37 fusion protein.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1:50, Immunocytochemistry: 1:200, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Aliases :

CDC 37; CDC37; CDC37 (cell division cycle 37 S. cerevisiae homolog); CDC37 (cell division cycle 37, S. cerevisiae, homolog); CDC37 cell division cycle 37 homolog; Cdc37 homolog; CDC37 protein; CDC37A; Cdc37p; Cell division control protein 37; cell division cycle 37; cell division cycle 37 homolog; hsp90 chaperone protein kinase-targeting subunit; Hsp90 co-chaperone Cdc37; Hsp90 co-chaperone Cdc37, N-terminally processed; p50; P50CDC37; SMO1; YD9489.03; YDR168W

more info or order :

Invitrogen product webpage