mtHSP70 Monoclonal Antibody (JG1) | Invitrogen MA3-028 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

mtHSP70 Monoclonal Antibody (JG1)

catalog :

MA3-028

quantity :

100 µL

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

JG1

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 125

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig 1e	Zhou S, Zhang W, Cai G, Ding Y, Wei C, Li S, et al. Myofiber necroptosis promotes muscle stem cell proliferation via releasing Tenascin-C during regeneration. Cell Res. 2020;30:1063-1077 pubmed publisher
western blot; mouse; fig 7a	Lim J, Lim J, Kim G, Levine R. Myristoylated methionine sulfoxide reductase A is a late endosomal protein. J Biol Chem. 2018;293:7355-7366 pubmed publisher
immunocytochemistry; human; loading ...; fig 2c	Terao Y, Fujita H, Horibe S, Sato J, Minami S, Kobayashi M, et al. Interaction of FAM5C with UDP-glucose:glycoprotein glucosyltransferase 1 (UGGT1): Implication of N-glycosylation in FAM5C secretion. Biochem Biophys Res Commun. 2017;486:811-816 pubmed publisher
western blot; human; fig 1a	Liu T, Krysiak K, Shirai C, Kim S, Shao J, Ndonwi M, et al. Knockdown of HSPA9 induces TP53-dependent apoptosis in human hematopoietic progenitor cells. PLoS ONE. 2017;12:e0170470 pubmed publisher
western blot; human; loading ...; fig 3c	Zaganelli S, Rebelo Guiomar P, Maundrell K, Rozanska A, Pierredon S, Powell C, et al. The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules. J Biol Chem. 2017;292:4519-4532 pubmed publisher
immunohistochemistry; rat; 1:50; loading ... western blot; rat; 1:500; loading ...; fig 8b	de la Fuente S, Fernandez Sanz C, Vail C, Agra E, Holmström K, Sun J, et al. Strategic Positioning and Biased Activity of the Mitochondrial Calcium Uniporter in Cardiac Muscle. J Biol Chem. 2016;291:23343-23362 pubmed
western blot; mouse; loading ...; fig 4h	Lacroix M, Rodier G, Kirsh O, Houles T, Delpech H, Seyran B, et al. E4F1 controls a transcriptional program essential for pyruvate dehydrogenase activity. Proc Natl Acad Sci U S A. 2016;113:10998-1003 pubmed publisher
western blot; human; loading ...; fig 1c	BRANDT C, McFie P, Stone S. Diacylglycerol acyltransferase-2 and monoacylglycerol acyltransferase-2 are ubiquitinated proteins that are degraded by the 26S proteasome. Biochem J. 2016;473:3621-3637 pubmed
western blot; human; fig 2	Vanderperre B, Cermakova K, Escoffier J, Kaba M, Bender T, Nef S, et al. MPC1-like Is a Placental Mammal-specific Mitochondrial Pyruvate Carrier Subunit Expressed in Postmeiotic Male Germ Cells. J Biol Chem. 2016;291:16448-61 pubmed publisher
western blot; human; loading ...; fig 2f	Akabane S, Matsuzaki K, Yamashita S, Arai K, Okatsu K, Kanki T, et al. Constitutive Activation of PINK1 Protein Leads to Proteasome-mediated and Non-apoptotic Cell Death Independently of Mitochondrial Autophagy. J Biol Chem. 2016;291:16162-74 pubmed publisher
western blot; human; fig 5	Lim S, Liu H, Madeira da Silva L, Arora R, Liu Z, Phillips J, et al. Immunoregulatory Protein B7-H3 Reprograms Glucose Metabolism in Cancer Cells by ROS-Mediated Stabilization of HIF1?. Cancer Res. 2016;76:2231-42 pubmed publisher
western blot; mouse; fig 6	Martin G, Chung S, Landrock D, Landrock K, Huang H, Dangott L, et al. FABP-1 gene ablation impacts brain endocannabinoid system in male mice. J Neurochem. 2016;138:407-22 pubmed publisher
western blot; human; 1:1000; fig 4	Gao S, Chen X, Jin H, Ren S, Liu Z, Fang X, et al. Overexpression of ErbB2 renders breast cancer cells susceptible to 3-BrPA through the increased dissociation of hexokinase II from mitochondrial outer membrane. Oncol Lett. 2016;11:1567-1573 pubmed
immunocytochemistry; human; fig 2	Min S, Kady J, Nam M, Rojas Rodriguez R, Berkenwald A, Kim J, et al. Human 'brite/beige' adipocytes develop from capillary networks, and their implantation improves metabolic homeostasis in mice. Nat Med. 2016;22:312-8 pubmed publisher
western blot; rat; 1:1000; fig 1	Torres G, Morales P, GarcÃa Miguel M, Norambuena Soto I, Cartes Saavedra B, Vidal PeÃ±a G, et al. Glucagon-like peptide-1 inhibits vascular smooth muscle cell dedifferentiation through mitochondrial dynamics regulation. Biochem Pharmacol. 2016;104:52-61 pubmed publisher
immunocytochemistry; human; 1:500; fig s1 western blot; human; 1:500; fig 1	Mills K, Brocardo M, Henderson B. APC binds the Miro/Milton motor complex to stimulate transport of mitochondria to the plasma membrane. Mol Biol Cell. 2016;27:466-82 pubmed publisher
western blot; mouse; fig 4	Isbel L, Srivastava R, Oey H, Spurling A, Daxinger L, Puthalakath H, et al. Trim33 Binds and Silences a Class of Young Endogenous Retroviruses in the Mouse Testis; a Novel Component of the Arms Race between Retrotransposons and the Host Genome. PLoS Genet. 2015;11:e1005693 pubmed publisher
western blot; rat	StanojloviÄ‡ M, GuÅ¡evac I, GrkoviÄ‡ I, ZlatkoviÄ‡ J, MitroviÄ‡ N, ZariÄ‡ M, et al. Effects of chronic cerebral hypoperfusion and low-dose progesterone treatment on apoptotic processes, expression and subcellular localization of key elements within Akt and Erk signaling pathways in rat hippocampus. Neuroscience. 2015;311:308-21 pubmed publisher
western blot; mouse; fig 1d	Naghdi S, VÃ¡rnai P, HajnÃ³czky G. Motifs of VDAC2 required for mitochondrial Bak import and tBid-induced apoptosis. Proc Natl Acad Sci U S A. 2015;112:E5590-9 pubmed publisher
immunocytochemistry; human; 1:100; fig 7	Nehra S, Bhardwaj V, Ganju L, Saraswat D. Nanocurcumin Prevents Hypoxia Induced Stress in Primary Human Ventricular Cardiomyocytes by Maintaining Mitochondrial Homeostasis. PLoS ONE. 2015;10:e0139121 pubmed publisher
western blot; human; fig 2a	Hilton B, Li Z, Musich P, Wang H, Cartwright B, SERRANO M, et al. ATR Plays a Direct Antiapoptotic Role at Mitochondria, which Is Regulated by Prolyl Isomerase Pin1. Mol Cell. 2015;60:35-46 pubmed publisher
immunocytochemistry; human	Vertii A, Zimmerman W, Ivshina M, Doxsey S. Centrosome-intrinsic mechanisms modulate centrosome integrity during fever. Mol Biol Cell. 2015;26:3451-63 pubmed publisher
western blot; human; 1:300	Tembe V, Martino Echarri E, Marzec K, Mok M, Brodie K, Mills K, et al. The BARD1 BRCT domain contributes to p53 binding, cytoplasmic and mitochondrial localization, and apoptotic function. Cell Signal. 2015;27:1763-71 pubmed publisher
western blot; mouse; fig 1	Randles M, Woolf A, Huang J, Byron A, Humphries J, Price K, et al. Genetic Background is a Key Determinant of Glomerular Extracellular Matrix Composition and Organization. J Am Soc Nephrol. 2015;26:3021-34 pubmed publisher
western blot; human	Yang N, Han F, Cui H, Huang J, Wang T, Zhou Y, et al. Matrine suppresses proliferation and induces apoptosis in human cholangiocarcinoma cells through suppression of JAK2/STAT3 signaling. Pharmacol Rep. 2015;67:388-93 pubmed publisher
western blot; mouse; 1:1000; fig 1	Robertson J, Jacquemet G, Byron A, Jones M, Warwood S, Selley J, et al. Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling. Nat Commun. 2015;6:6265 pubmed publisher
western blot; mouse; fig 1d	Krysiak K, Tibbitts J, Shao J, Liu T, Ndonwi M, Walter M. Reduced levels of Hspa9 attenuate Stat5 activation in mouse B cells. Exp Hematol. 2015;43:319-30.e10 pubmed publisher
western blot; human	Hu Z, Zeng Q, Zhang B, Liu H, Wang W. Promotion of p53 expression and reactive oxidative stress production is involved in zerumbone-induced cisplatin sensitization of non-small cell lung cancer cells. Biochimie. 2014;107 Pt B:257-62 pubmed publisher
immunocytochemistry; human; fig 5	Odendall C, Dixit E, Stavru F, Bierne H, Franz K, Durbin A, et al. Diverse intracellular pathogens activate type III interferon expression from peroxisomes. Nat Immunol. 2014;15:717-26 pubmed publisher
western blot; mouse	Rahimi A, Lee Y, Abdella H, Doerflinger M, Gangoda L, Srivastava R, et al. Role of p53 in cAMP/PKA pathway mediated apoptosis. Apoptosis. 2013;18:1492-9 pubmed publisher
western blot; rat	Adzic M, Lukic I, Mitic M, Djordjevic J, Elaković I, Djordjevic A, et al. Brain region- and sex-specific modulation of mitochondrial glucocorticoid receptor phosphorylation in fluoxetine treated stressed rats: effects on energy metabolism. Psychoneuroendocrinology. 2013;38:2914-24 pubmed publisher
western blot; mouse; 1:1000	Nair R, Lauks J, Jung S, Cooke N, de Wit H, Brose N, et al. Neurobeachin regulates neurotransmitter receptor trafficking to synapses. J Cell Biol. 2013;200:61-80 pubmed publisher
western blot; human; fig 2	Ding Y, Liu Z, Desai S, Zhao Y, Liu H, Pannell L, et al. Receptor tyrosine kinase ErbB2 translocates into mitochondria and regulates cellular metabolism. Nat Commun. 2012;3:1271 pubmed publisher
western blot; mouse	Brooks R, Williamson R, Bass M. Syndecan-4 independently regulates multiple small GTPases to promote fibroblast migration during wound healing. Small Gtpases. 2012;3:73-9 pubmed publisher
immunoprecipitation; human western blot; human	Gestl E, Anne Böttger S. Cytoplasmic sequestration of the tumor suppressor p53 by a heat shock protein 70 family member, mortalin, in human colorectal adenocarcinoma cell lines. Biochem Biophys Res Commun. 2012;423:411-6 pubmed publisher
western blot; human	Rinaldo C, Moncada A, Gradi A, Ciuffini L, D Eliseo D, Siepi F, et al. HIPK2 controls cytokinesis and prevents tetraploidization by phosphorylating histone H2B at the midbody. Mol Cell. 2012;47:87-98 pubmed publisher
western blot; mouse	Jeanes A, Wang P, Moreno Layseca P, Paul N, Cheung J, Tsang R, et al. Specific ?-containing integrins exert differential control on proliferation and two-dimensional collective cell migration in mammary epithelial cells. J Biol Chem. 2012;287:24103-12 pubmed publisher
western blot; mouse; 1:5000	Djordjevic A, Djordjevic J, Elaković I, Adzic M, Matić G, Radojcic M. Fluoxetine affects hippocampal plasticity, apoptosis and depressive-like behavior of chronically isolated rats. Prog Neuropsychopharmacol Biol Psychiatry. 2012;36:92-100 pubmed publisher
western blot; human	Chung Y, Kim H, Kim I, Yim M, Chock P. Dual function of protein kinase C (PKC) in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced manganese superoxide dismutase (MnSOD) expression: activation of CREB and FOXO3a by PKC-alpha phosphorylation and by PKC-mediated inactivation of Akt, respe. J Biol Chem. 2011;286:29681-90 pubmed publisher
immunocytochemistry; human	Vanderperre B, Staskevicius A, Tremblay G, McCoy M, O Neill M, Cashman N, et al. An overlapping reading frame in the PRNP gene encodes a novel polypeptide distinct from the prion protein. FASEB J. 2011;25:2373-86 pubmed publisher
immunocytochemistry; human western blot; human	Trinh D, Elwi A, Kim S. Direct interaction between p53 and Tid1 proteins affects p53 mitochondrial localization and apoptosis. Oncotarget. 2010;1:396-404 pubmed
western blot; human	Chen X, Wong J, Wong P, Radany E. Low-dose valproic acid enhances radiosensitivity of prostate cancer through acetylated p53-dependent modulation of mitochondrial membrane potential and apoptosis. Mol Cancer Res. 2011;9:448-61 pubmed publisher
western blot; mouse western blot; human	Chen T, Kambal A, Krysiak K, Walshauser M, Raju G, Tibbitts J, et al. Knockdown of Hspa9, a del(5q31.2) gene, results in a decrease in hematopoietic progenitors in mice. Blood. 2011;117:1530-9 pubmed publisher
western blot; human	Amadoro G, Corsetti V, Stringaro A, Colone M, D Aguanno S, Meli G, et al. A NH2 tau fragment targets neuronal mitochondria at AD synapses: possible implications for neurodegeneration. J Alzheimers Dis. 2010;21:445-70 pubmed publisher
western blot; human; fig 1c	Irvine M, Philipsz S, Frausto M, Mijatov B, Gallagher S, Fung C, et al. Amino terminal hydrophobic import signals target the p14(ARF) tumor suppressor to the mitochondria. Cell Cycle. 2010;9:829-39 pubmed
immunocytochemistry; mouse; 1:250 western blot; mouse	Cole N, Daniels M, Levine R, Kim G. Oxidative stress causes reversible changes in mitochondrial permeability and structure. Exp Gerontol. 2010;45:596-602 pubmed publisher
western blot; rat; 2.32 ug/ml	Alvarez Delgado C, Mendoza Rodriguez C, Picazo O, Cerbón M. Different expression of alpha and beta mitochondrial estrogen receptors in the aging rat brain: interaction with respiratory complex V. Exp Gerontol. 2010;45:580-5 pubmed publisher
western blot; rat	Djordjevic J, Djordjevic A, Adzic M, Radojcic M. Chronic social isolation compromises the activity of both glutathione peroxidase and catalase in hippocampus of male wistar rats. Cell Mol Neurobiol. 2010;30:693-700 pubmed publisher
western blot; human	Chen X, Wong P, Radany E, Wong J. HDAC inhibitor, valproic acid, induces p53-dependent radiosensitization of colon cancer cells. Cancer Biother Radiopharm. 2009;24:689-99 pubmed publisher
immunocytochemistry; mouse western blot; mouse	Owens T, Foster F, Valentijn A, Gilmore A, Streuli C. Role for X-linked Inhibitor of apoptosis protein upstream of mitochondrial permeabilization. J Biol Chem. 2010;285:1081-8 pubmed publisher
western blot; rat	Adzic M, Djordjevic A, Demonacos C, Krstic Demonacos M, Radojcic M. The role of phosphorylated glucocorticoid receptor in mitochondrial functions and apoptotic signalling in brain tissue of stressed Wistar rats. Int J Biochem Cell Biol. 2009;41:2181-8 pubmed publisher
western blot; rat	Li Y, Bondada V, Joshi A, Geddes J. Calpain 1 and Calpastatin expression is developmentally regulated in rat brain. Exp Neurol. 2009;220:316-9 pubmed publisher
western blot; human	Humphries J, Byron A, Bass M, Craig S, Pinney J, Knight D, et al. Proteomic analysis of integrin-associated complexes identifies RCC2 as a dual regulator of Rac1 and Arf6. Sci Signal. 2009;2:ra51 pubmed publisher
western blot; human; 1:10000	Jodoin J, Misiewicz M, Makhijani P, Giannopoulos P, Hammond J, Goodyer C, et al. Loss of anti-Bax function in Gerstmann-Sträussler-Scheinker syndrome-associated prion protein mutants. PLoS ONE. 2009;4:e6647 pubmed publisher
western blot; rat	Djordjevic A, Adzic M, Djordjevic J, Radojcic M. Chronic social isolation is related to both upregulation of plasticity genes and initiation of proapoptotic signaling in Wistar rat hippocampus. J Neural Transm (Vienna). 2009;116:1579-89 pubmed publisher
immunocytochemistry; mouse western blot; mouse; fig 2d	Owens T, Valentijn A, Upton J, Keeble J, Zhang L, Lindsay J, et al. Apoptosis commitment and activation of mitochondrial Bax during anoikis is regulated by p38MAPK. Cell Death Differ. 2009;16:1551-62 pubmed publisher
western blot; mouse	Mancini F, Di Conza G, Pellegrino M, Rinaldo C, Prodosmo A, Giglio S, et al. MDM4 (MDMX) localizes at the mitochondria and facilitates the p53-mediated intrinsic-apoptotic pathway. EMBO J. 2009;28:1926-39 pubmed publisher
western blot; human	Ngo J, Davies K. Mitochondrial Lon protease is a human stress protein. Free Radic Biol Med. 2009;46:1042-8 pubmed publisher
western blot; human; 1:1000	Laroche Pierre S, Jodoin J, LeBlanc A. Helix 3 is necessary and sufficient for prion protein's anti-Bax function. J Neurochem. 2009;108:1019-31 pubmed publisher
immunocytochemistry; human western blot; human	Parcellier A, Tintignac L, Zhuravleva E, Cron P, Schenk S, Bozulic L, et al. Carboxy-Terminal Modulator Protein (CTMP) is a mitochondrial protein that sensitizes cells to apoptosis. Cell Signal. 2009;21:639-50 pubmed publisher
western blot; human; 1:500	Klaiman G, Petzke T, Hammond J, LeBlanc A. Targets of caspase-6 activity in human neurons and Alzheimer disease. Mol Cell Proteomics. 2008;7:1541-55 pubmed publisher
immunocytochemistry; mouse western blot; mouse	Valentijn A, Upton J, Bates N, Gilmore A. Bax targeting to mitochondria occurs via both tail anchor-dependent and -independent mechanisms. Cell Death Differ. 2008;15:1243-54 pubmed publisher
western blot; human; 1:500	Fujita Y, Nakanishi T, Miyamoto Y, Hiramatsu M, Mabuchi H, Miyamoto A, et al. Proteomics-based identification of autoantibody against heat shock protein 70 as a diagnostic marker in esophageal squamous cell carcinoma. Cancer Lett. 2008;263:280-90 pubmed publisher
immunocytochemistry; human	Tang X, Zhu Y, Han L, Kim A, Kopelovich L, Bickers D, et al. CP-31398 restores mutant p53 tumor suppressor function and inhibits UVB-induced skin carcinogenesis in mice. J Clin Invest. 2007;117:3753-64 pubmed
western blot; rat; 1:5000	Sirk D, Zhu Z, Wadia J, Shulyakova N, Phan N, Fong J, et al. Chronic exposure to sub-lethal beta-amyloid (Abeta) inhibits the import of nuclear-encoded proteins to mitochondria in differentiated PC12 cells. J Neurochem. 2007;103:1989-2003 pubmed
immunocytochemistry; mouse; 1:300	Dowling A, Waterfield N, Hares M, Le Goff G, Streuli C, Ffrench Constant R. The Mcf1 toxin induces apoptosis via the mitochondrial pathway and apoptosis is attenuated by mutation of the BH3-like domain. Cell Microbiol. 2007;9:2470-84 pubmed
western blot; human	Ahuja N, Schwer B, Carobbio S, Waltregny D, North B, Castronovo V, et al. Regulation of insulin secretion by SIRT4, a mitochondrial ADP-ribosyltransferase. J Biol Chem. 2007;282:33583-92 pubmed
western blot; human; 1:1000	Jodoin J, Laroche Pierre S, Goodyer C, LeBlanc A. Defective retrotranslocation causes loss of anti-Bax function in human familial prion protein mutants. J Neurosci. 2007;27:5081-91 pubmed
immunocytochemistry; mouse	Upton J, Valentijn A, Zhang L, Gilmore A. The N-terminal conformation of Bax regulates cell commitment to apoptosis. Cell Death Differ. 2007;14:932-42 pubmed
western blot; mouse; 1:500	Cheung E, Joza N, Steenaart N, McClellan K, Neuspiel M, McNamara S, et al. Dissociating the dual roles of apoptosis-inducing factor in maintaining mitochondrial structure and apoptosis. EMBO J. 2006;25:4061-73 pubmed
immunocytochemistry; human; 1:50	Grenier C, Bissonnette C, Volkov L, Roucou X. Molecular morphology and toxicity of cytoplasmic prion protein aggregates in neuronal and non-neuronal cells. J Neurochem. 2006;97:1456-66 pubmed
western blot; human	Nemajerova A, Wolff S, Petrenko O, Moll U. Viral and cellular oncogenes induce rapid mitochondrial translocation of p53 in primary epithelial and endothelial cells early in apoptosis. FEBS Lett. 2005;579:6079-83 pubmed
western blot; human	Roucou X, Giannopoulos P, Zhang Y, Jodoin J, Goodyer C, Leblanc A. Cellular prion protein inhibits proapoptotic Bax conformational change in human neurons and in breast carcinoma MCF-7 cells. Cell Death Differ. 2005;12:783-95 pubmed
western blot; human	Kobayashi S, Kajino S, Takahashi N, Kanazawa S, Imai K, Hibi Y, et al. 53BP2 induces apoptosis through the mitochondrial death pathway. Genes Cells. 2005;10:253-60 pubmed
western blot; human; fig 4	Desagher S, Osen Sand A, Nichols A, Eskes R, Montessuit S, Lauper S, et al. Bid-induced conformational change of Bax is responsible for mitochondrial cytochrome c release during apoptosis. J Cell Biol. 1999;144:891-901 pubmed
	Monsálves Álvarez M, Morales P, Castro Sepúlveda M, Sepúlveda C, Rodriguez J, Chiong M, et al. β-Hydroxybutyrate Increases Exercise Capacity Associated with Changes in Mitochondrial Function in Skeletal Muscle. Nutrients. 2020;12: pubmed publisher
	Debattisti V, Horn A, Singh R, Seifert E, Hogarth M, Mazala D, et al. Dysregulation of Mitochondrial Ca2+ Uptake and Sarcolemma Repair Underlie Muscle Weakness and Wasting in Patients and Mice Lacking MICU1. Cell Rep. 2019;29:1274-1286.e6 pubmed publisher
	Ivanova I, Perkins N. Hypoxia induces rapid, STAT3 and ROS dependent, mitochondrial translocation of RelA(p65) and IκBα. Biosci Rep. 2019;39: pubmed publisher
	Lomash R, Petralia R, Holtzclaw L, Tsuda M, Wang Y, Badger J, et al. Neurolastin, a dynamin family GTPase, translocates to mitochondria upon neuronal stress and alters mitochondrial morphology in vivo. J Biol Chem. 2019;294:11498-11512 pubmed publisher
	Patten D, Ouellet M, Allan D, Germain M, Baird S, Harper M, et al. Mitochondrial adaptation in human mesenchymal stem cells following ionizing radiation. FASEB J. 2019;33:9263-9278 pubmed publisher
	BRANDT C, McFie P, Vu H, Chumala P, Katselis G, Stone S. Identification of calnexin as a diacylglycerol acyltransferase-2 interacting protein. PLoS ONE. 2019;14:e0210396 pubmed publisher
	Patil H, Yoon D, Bhowmick R, Cai Y, Cho K, Ferreira P. Impairments in age-dependent ubiquitin proteostasis and structural integrity of selective neurons by uncoupling Ran GTPase from the Ran-binding domain 3 of Ranbp2 and identification of novel mitochondrial isoforms of ubiquitin-conjugating enzyme E2I . Small Gtpases. 2019;10:146-161 pubmed publisher
	Naghdi S, Slovinsky W, Madesh M, Rubin E, Hajnoczky G. Mitochondrial fusion and Bid-mediated mitochondrial apoptosis are perturbed by alcohol with distinct dependence on its metabolism. Cell Death Dis. 2018;9:1028 pubmed publisher
	Delcourt V, Brunelle M, Roy A, Jacques J, Salzet M, Fournier I, et al. The Protein Coded by a Short Open Reading Frame, Not by the Annotated Coding Sequence, Is the Main Gene Product of the Dual-Coding Gene MIEF1. Mol Cell Proteomics. 2018;17:2402-2411 pubmed publisher
	Dunham J, van de Vis R, Bauer J, Wubben J, van Driel N, Laman J, et al. Severe oxidative stress in an acute inflammatory demyelinating model in the rhesus monkey. PLoS ONE. 2017;12:e0188013 pubmed publisher
	Hou S, Madoux F, Scampavia L, Janovick J, Conn P, Spicer T. Drug Library Screening for the Identification of Ionophores That Correct the Mistrafficking Disorder Associated with Oxalosis Kidney Disease. SLAS Discov. 2017;22:887-896 pubmed publisher
	Rohlenova K, Sachaphibulkij K, Stursa J, Bezawork Geleta A, Blecha J, Endaya B, et al. Selective Disruption of Respiratory Supercomplexes as a New Strategy to Suppress Her2high Breast Cancer. Antioxid Redox Signal. 2017;26:84-103 pubmed publisher
	Jin S, Youle R. The accumulation of misfolded proteins in the mitochondrial matrix is sensed by PINK1 to induce PARK2/Parkin-mediated mitophagy of polarized mitochondria. Autophagy. 2013;9:1750-7 pubmed publisher
	Grishchuk Y, Ginet V, Truttmann A, Clarke P, Puyal J. Beclin 1-independent autophagy contributes to apoptosis in cortical neurons. Autophagy. 2011;7:1115-31 pubmed publisher
	Rardin M, Wiley S, Murphy A, Pagliarini D, Dixon J. Dual specificity phosphatases 18 and 21 target to opposing sides of the mitochondrial inner membrane. J Biol Chem. 2008;283:15440-50 pubmed publisher
	Yi H, Friedman J, Ferreira P. The cyclophilin-like domain of Ran-binding protein-2 modulates selectively the activity of the ubiquitin-proteasome system and protein biogenesis. J Biol Chem. 2007;282:34770-8 pubmed
	Goetz J, Genty H, St Pierre P, Dang T, Joshi B, Sauve R, et al. Reversible interactions between smooth domains of the endoplasmic reticulum and mitochondria are regulated by physiological cytosolic Ca2+ levels. J Cell Sci. 2007;120:3553-64 pubmed
	Widodo N, Kaur K, Shrestha B, Takagi Y, Ishii T, Wadhwa R, et al. Selective killing of cancer cells by leaf extract of Ashwagandha: identification of a tumor-inhibitory factor and the first molecular insights to its effect. Clin Cancer Res. 2007;13:2298-306 pubmed
	Schwer B, Bunkenborg J, Verdin R, Andersen J, Verdin E. Reversible lysine acetylation controls the activity of the mitochondrial enzyme acetyl-CoA synthetase 2. Proc Natl Acad Sci U S A. 2006;103:10224-10229 pubmed publisher
	Lu B, Garrido N, Spelbrink J, Suzuki C. Tid1 isoforms are mitochondrial DnaJ-like chaperones with unique carboxyl termini that determine cytosolic fate. J Biol Chem. 2006;281:13150-8 pubmed
	Tomita Y, Marchenko N, Erster S, Nemajerova A, Dehner A, Klein C, et al. WT p53, but not tumor-derived mutants, bind to Bcl2 via the DNA binding domain and induce mitochondrial permeabilization. J Biol Chem. 2006;281:8600-6 pubmed
	Korenaga M, Wang T, Li Y, Showalter L, Chan T, Sun J, et al. Hepatitis C virus core protein inhibits mitochondrial electron transport and increases reactive oxygen species (ROS) production. J Biol Chem. 2005;280:37481-8 pubmed
	Jia Z, Barbier L, Stuart H, Amraei M, Pelech S, Dennis J, et al. Tumor cell pseudopodial protrusions. Localized signaling domains coordinating cytoskeleton remodeling, cell adhesion, glycolysis, RNA translocation, and protein translation. J Biol Chem. 2005;280:30564-73 pubmed
	Xie B, Li H, Wang Q, Xie S, Rahmeh A, Dai W, et al. Further characterization of human DNA polymerase delta interacting protein 38. J Biol Chem. 2005;280:22375-84 pubmed
	Wang J, Ladrech S, Pujol R, Brabet P, Van De Water T, Puel J. Caspase inhibitors, but not c-Jun NH2-terminal kinase inhibitor treatment, prevent cisplatin-induced hearing loss. Cancer Res. 2004;64:9217-24 pubmed
	Kaur K, Rani G, Widodo N, Nagpal A, Taira K, Kaul S, et al. Evaluation of the anti-proliferative and anti-oxidative activities of leaf extract from in vivo and in vitro raised Ashwagandha. Food Chem Toxicol. 2004;42:2015-20 pubmed
	Ugalde C, Vogel R, Huijbens R, van den Heuvel B, Smeitink J, Nijtmans L. Human mitochondrial complex I assembles through the combination of evolutionary conserved modules: a framework to interpret complex I deficiencies. Hum Mol Genet. 2004;13:2461-72 pubmed
	Erster S, Mihara M, Kim R, Petrenko O, Moll U. In vivo mitochondrial p53 translocation triggers a rapid first wave of cell death in response to DNA damage that can precede p53 target gene activation. Mol Cell Biol. 2004;24:6728-41 pubmed
	Schwer B, Ren S, Pietschmann T, Kartenbeck J, Kaehlcke K, Bartenschlager R, et al. Targeting of hepatitis C virus core protein to mitochondria through a novel C-terminal localization motif. J Virol. 2004;78:7958-68 pubmed
	Valentijn A, Gilmore A. Translocation of full-length Bid to mitochondria during anoikis. J Biol Chem. 2004;279:32848-57 pubmed
	Lo J, Hayashi M, Woo Kim S, Tian B, Huang J, Fearns C, et al. Tid1, a cochaperone of the heat shock 70 protein and the mammalian counterpart of the Drosophila tumor suppressor l(2)tid, is critical for early embryonic development and cell survival. Mol Cell Biol. 2004;24:2226-36 pubmed
	Nishitai G, Shimizu N, Negishi T, Kishimoto H, Nakagawa K, Kitagawa D, et al. Stress induces mitochondria-mediated apoptosis independent of SAPK/JNK activation in embryonic stem cells. J Biol Chem. 2004;279:1621-6 pubmed
	Ventura A, Maccarana M, Raker V, Pelicci P. A cryptic targeting signal induces isoform-specific localization of p46Shc to mitochondria. J Biol Chem. 2004;279:2299-306 pubmed
	Ribeiro C, Paradiso A, Livraghi A, Boucher R. The mitochondrial barriers segregate agonist-induced calcium-dependent functions in human airway epithelia. J Gen Physiol. 2003;122:377-87 pubmed
	Valentijn A, Metcalfe A, Kott J, Streuli C, Gilmore A. Spatial and temporal changes in Bax subcellular localization during anoikis. J Cell Biol. 2003;162:599-612 pubmed
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image

image 1 :

Immunofluorescent analysis of Heat Shock Protein 70 using anti-Heat Shock Protein 70 monoclonal antibody (Product# MA3-028) shows staining in HMVEC Cells.

product information

Product Type :

Antibody

Product Name :

mtHSP70 Monoclonal Antibody (JG1)

Catalog # :

MA3-028

Quantity :

100 µL

Price :

US 399.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Canine, Human, Mouse, Non-human primate

Applications :

Immunocytochemistry: 1:50, Immunohistochemistry (Paraffin): 1 µg/mL, Immunoprecipitation: Assay-dependent, Western Blot: 1:500

Species :

Canine, Human, Mouse, Non-human primate

Clone :

JG1

Isotype :

IgG3

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a 70 kDa protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein which is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa constitutively expressed protein which is involved in uncoating clathrin-coated vesicles; GRP78, or BiP, is a glucose-regulated 78 kDa protein localized to the endoplasmic reticulum; and mitochondrial HSP70 (mtHSP70, GRP75 or mortalin) a 75 kDa protein that is found within the mitochondria. mtHSP70 is a mitochondrial resident protein that is involved in protein translocation into the mitochondria. Preproteins cross the mitochondrial membranes in an extended conformation. This requires unfolding of preproteins before entering translocation pores in the mitochondrial outer membrane. Preprotein unfolding is thought to be mediated by mtHSP70 and other inner membrane proteins of the mitochondria.

Immunogen :

Synthetic peptide corresponding to residues G(615) S G S S G T G E Q K E D Q K E E K Q(633) of mouse mtHSP70.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:50, Immunohistochemistry (Paraffin): 1 µg/mL, Immunoprecipitation: Assay-dependent, Western Blot: 1:500

Aliases :

68 kDa heat shock protein; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock protein 1A; heat shock protein family A (Hsp70) member 1A; heat shock protein, 70 kDa 3; heat shock-induced protein; HEL-S-103; Hsp68; HSP70.1; HSP70.1/HSP70.2; Hsp70.3; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-2; Hsp70-3; Hsp70A1; HSP70I; Hsp72; HSPA1; Hspa1a; HSPA1B; HSX70; inducible heat shock protein 70

more info or order :

Invitrogen product webpage