Calnexin Monoclonal Antibody (AF18) | Invitrogen MA3-027 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Calnexin Monoclonal Antibody (AF18)

catalog :

MA3-027

quantity :

100 µL

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AF18

reactivity :

African green monkey, human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section, RNA immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 48

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig s9	Torgersen M, Klokk T, Kavaliauskiene S, Klose C, Simons K, Skotland T, et al. The anti-tumor drug 2-hydroxyoleic acid (Minerval) stimulates signaling and retrograde transport. Oncotarget. 2016;7:86871-86888 pubmed publisher
immunocytochemistry; human; fig s4	Kajiho H, Kajiho Y, Frittoli E, Confalonieri S, Bertalot G, Viale G, et al. RAB2A controls MT1-MMP endocytic and E-cadherin polarized Golgi trafficking to promote invasive breast cancer programs. EMBO Rep. 2016;17:1061-80 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:40	Theodorou M, Rauser B, Zhang J, Prakash N, Wurst W, Schick J. Limitations of In Vivo Reprogramming to Dopaminergic Neurons via a Tricistronic Strategy. Hum Gene Ther Methods. 2015;26:107-22 pubmed publisher
western blot; rat; 1:10000	Bejarano E, Yuste A, Patel B, Stout R, Spray D, Cuervo A. Connexins modulate autophagosome biogenesis. Nat Cell Biol. 2014;16:401-14 pubmed publisher
western blot; mouse	Wei P, Hsieh Y, Su M, Jiang X, Hsu P, Lo W, et al. Loss of the oxidative stress sensor NPGPx compromises GRP78 chaperone activity and induces systemic disease. Mol Cell. 2012;48:747-59 pubmed publisher
western blot; human; fig 1	Wei P, Lo W, Su M, Shew J, Lee W. Non-targeting siRNA induces NPGPx expression to cooperate with exoribonuclease XRN2 for releasing the stress. Nucleic Acids Res. 2012;40:323-32 pubmed publisher
western blot; human	Zhong Y, Wang Y, Yang H, Ballar P, Lee J, Ye Y, et al. Importin beta interacts with the endoplasmic reticulum-associated degradation machinery and promotes ubiquitination and degradation of mutant alpha1-antitrypsin. J Biol Chem. 2011;286:33921-30 pubmed publisher
western blot; mouse; 1:1000	Lee J, Yu W, Kumar A, Lee S, Mohan P, Peterhoff C, et al. Lysosomal proteolysis and autophagy require presenilin 1 and are disrupted by Alzheimer-related PS1 mutations. Cell. 2010;141:1146-58 pubmed publisher
western blot; African green monkey	Däubner T, Fink A, Seitz A, Tenzer S, Müller J, Strand D, et al. A novel transmembrane domain mediating retention of a highly motile herpesvirus glycoprotein in the endoplasmic reticulum. J Gen Virol. 2010;91:1524-34 pubmed publisher
immunocytochemistry; human; 1:300	Petersson J, Lönnbro P, Herr A, Morgelin M, Gullberg U, Drott K. The human IFN-inducible p53 target gene TRIM22 colocalizes with the centrosome independently of cell cycle phase. Exp Cell Res. 2010;316:568-79 pubmed publisher
western blot; human	Brasaemle D, Wolins N. Isolation of lipid droplets from cells by density gradient centrifugation. Curr Protoc Cell Biol. 2006;Chapter 3:Unit 3.15 pubmed publisher
western blot; human	Medigeshi G, Lancaster A, Hirsch A, Briese T, Lipkin W, DeFilippis V, et al. West Nile virus infection activates the unfolded protein response, leading to CHOP induction and apoptosis. J Virol. 2007;81:10849-60 pubmed
western blot; human	Marrocco D, Tilley W, Bianco Miotto T, Evdokiou A, Scher H, Rifkind R, et al. Suberoylanilide hydroxamic acid (vorinostat) represses androgen receptor expression and acts synergistically with an androgen receptor antagonist to inhibit prostate cancer cell proliferation. Mol Cancer Ther. 2007;6:51-60 pubmed
immunocytochemistry; human	Zhang H, Schmidt B, Sun F, Condliffe S, Butterworth M, Youker R, et al. Cysteine string protein monitors late steps in cystic fibrosis transmembrane conductance regulator biogenesis. J Biol Chem. 2006;281:11312-21 pubmed
immunohistochemistry; mouse; 1:100	Kerov V, Natochin M, Artemyev N. Interaction of transducin-alpha with LGN, a G-protein modulator expressed in photoreceptor cells. Mol Cell Neurosci. 2005;28:485-95 pubmed
immunocytochemistry; human	Barre L, Magdalou J, Netter P, Fournel Gigleux S, Ouzzine M. The stop transfer sequence of the human UDP-glucuronosyltransferase 1A determines localization to the endoplasmic reticulum by both static retention and retrieval mechanisms. FEBS J. 2005;272:1063-71 pubmed
	Hong X, Li Z, Hou J, Zhang H, Zhang C, Zhang J, et al. Effects of ER-resident and secreted AGR2 on cell proliferation, migration, invasion, and survival in PANC-1 pancreatic cancer cells. BMC Cancer. 2021;21:33 pubmed publisher
	Wang Q, Zhao Y, Zheng H, Wang Q, Wang W, Liu B, et al. CCDC170 affects breast cancer apoptosis through IRE1 pathway. Aging (Albany NY). 2020;13:1332-1356 pubmed publisher
	Solek P, Koszla O, Mytych J, Badura J, Chelminiak Z, Cuprys M, et al. Neuronal life or death linked to depression treatment: the interplay between drugs and their stress-related outcomes relate to single or combined drug therapies. Apoptosis. 2019;24:773-784 pubmed publisher
	Klaver E, Zhao P, May M, Flanagan Steet H, Freeze H, Gilmore R, et al. Selective inhibition of N-linked glycosylation impairs receptor tyrosine kinase processing. Dis Model Mech. 2019;: pubmed publisher
	Han D, Xu L, Liu P, Liu Y, Sun C, Yin Y. Allicin disrupts cardiac Cav1.2 channels via trafficking. Pharm Biol. 2019;57:245-249 pubmed publisher
	Yin Y, Garcia M, Novak A, Saunders A, Ank R, Nam A, et al. Surf4 (Erv29p) binds amino-terminal tripeptide motifs of soluble cargo proteins with different affinities, enabling prioritization of their exit from the endoplasmic reticulum. PLoS Biol. 2018;16:e2005140 pubmed publisher
	Canzoneri R, Rabassa M, Gurruchaga A, Ferretti V, Palma S, Isla Larrain M, et al. Alternative splicing variant of RHBDD2 is associated with cell stress response and breast cancer progression. Oncol Rep. 2018;40:909-915 pubmed publisher
	Ravera S, Signorello M, Bartolucci M, Ferrando S, Manni L, Caicci F, et al. Extramitochondrial energy production in platelets. Biol Cell. 2018;110:97-108 pubmed publisher
	Fan J, Wang Y, Liu L, Zhang H, Zhang F, Shi L, et al. cTAGE5 deletion in pancreatic β cells impairs proinsulin trafficking and insulin biogenesis in mice. J Cell Biol. 2017;216:4153-4164 pubmed publisher
	Willy J, Young S, Mosley A, Gawrieh S, Stevens J, Masuoka H, et al. Function of inhibitor of Bruton's tyrosine kinase isoform ? (IBTK?) in nonalcoholic steatohepatitis links autophagy and the unfolded protein response. J Biol Chem. 2017;292:14050-14065 pubmed publisher
	Horn A, Celic I, Dong C, Martirosyan I, Han J. A conserved role for the ESCRT membrane budding complex in LINE retrotransposition. PLoS Genet. 2017;13:e1006837 pubmed publisher
	Qiu J, Yu K, Fei X, Liu Y, Nakayasu E, Piehowski P, et al. A unique deubiquitinase that deconjugates phosphoribosyl-linked protein ubiquitination. Cell Res. 2017;27:865-881 pubmed publisher
	Serfass J, Takahashi Y, Zhou Z, Kawasawa Y, Liu Y, Tsotakos N, et al. Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection. J Biol Chem. 2017;292:10097-10111 pubmed publisher
	Taylor A, Melton J, Herrero L, Thaa B, Karo Astover L, Gage P, et al. Effects of an In-Frame Deletion of the 6k Gene Locus from the Genome of Ross River Virus. J Virol. 2016;90:4150-4159 pubmed publisher
	Schaefer N, Kluck C, Price K, Meiselbach H, Vornberger N, Schwarzinger S, et al. Disturbed neuronal ER-Golgi sorting of unassembled glycine receptors suggests altered subcellular processing is a cause of human hyperekplexia. J Neurosci. 2015;35:422-37 pubmed publisher
	Chin K, Zhou H, Wong C, Lee J, Chan C, Qiang B, et al. The liver-enriched transcription factor CREB-H is a growth suppressor protein underexpressed in hepatocellular carcinoma. Nucleic Acids Res. 2005;33:1859-73 pubmed
	Khanna R, Lee E, Papazian D. Transient calnexin interaction confers long-term stability on folded K+ channel protein in the ER. J Cell Sci. 2004;117:2897-908 pubmed
	Krumerman A, Gao X, Bian J, Melman Y, Kagan A, McDonald T. An LQT mutant minK alters KvLQT1 trafficking. Am J Physiol Cell Physiol. 2004;286:C1453-63 pubmed
	Enninga J, Levay A, Fontoura B. Sec13 shuttles between the nucleus and the cytoplasm and stably interacts with Nup96 at the nuclear pore complex. Mol Cell Biol. 2003;23:7271-84 pubmed
	Calvert M, Digilio L, Herr J, Coonrod S. Oolemmal proteomics--identification of highly abundant heat shock proteins and molecular chaperones in the mature mouse egg and their localization on the plasma membrane. Reprod Biol Endocrinol. 2003;1:27 pubmed
	Offterdinger M, Schöfer C, Weipoltshammer K, Grunt T. c-erbB-3: a nuclear protein in mammary epithelial cells. J Cell Biol. 2002;157:929-39 pubmed
	Zhang H, Peters K, Sun F, Marino C, Lang J, Burgoyne R, et al. Cysteine string protein interacts with and modulates the maturation of the cystic fibrosis transmembrane conductance regulator. J Biol Chem. 2002;277:28948-58 pubmed
	Desai B, Myers B, Schreiber S. FKBP12-rapamycin-associated protein associates with mitochondria and senses osmotic stress via mitochondrial dysfunction. Proc Natl Acad Sci U S A. 2002;99:4319-24 pubmed
	Li P, Nakanishi A, Clark S, Kasamatsu H. Formation of transitory intrachain and interchain disulfide bonds accompanies the folding and oligomerization of simian virus 40 Vp1 in the cytoplasm. Proc Natl Acad Sci U S A. 2002;99:1353-8 pubmed
	Ridsdale R, Tseu I, Wang J, Post M. CTP:phosphocholine cytidylyltransferase alpha is a cytosolic protein in pulmonary epithelial cells and tissues. J Biol Chem. 2001;276:49148-55 pubmed
	Khanna R, Myers M, Laine M, Papazian D. Glycosylation increases potassium channel stability and surface expression in mammalian cells. J Biol Chem. 2001;276:34028-34 pubmed
	Walenta J, Didier A, Liu X, Kramer H. The Golgi-associated hook3 protein is a member of a novel family of microtubule-binding proteins. J Cell Biol. 2001;152:923-34 pubmed
	Khoo K, Han M, Park J, Chae S, Kim U, Lee H, et al. Localization of the cyclic ADP-ribose-dependent calcium signaling pathway in hepatocyte nucleus. J Biol Chem. 2000;275:24807-17 pubmed
	Meacham G, Lu Z, King S, Sorscher E, Tousson A, Cyr D. The Hdj-2/Hsc70 chaperone pair facilitates early steps in CFTR biogenesis. EMBO J. 1999;18:1492-505 pubmed
	Lindquist J, Jensen O, Mann M, Hammerling G. ER-60, a chaperone with thiol-dependent reductase activity involved in MHC class I assembly. EMBO J. 1998;17:2186-95 pubmed
	Rajagopalan S, Xu Y, Brenner M. Retention of unassembled components of integral membrane proteins by calnexin. Science. 1994;263:387-90 pubmed
	Galvin K, Krishna S, Ponchel F, Frohlich M, Cummings D, Carlson R, et al. The major histocompatibility complex class I antigen-binding protein p88 is the product of the calnexin gene. Proc Natl Acad Sci U S A. 1992;89:8452-6 pubmed

image

image 1 :

Immunohistochemistry was performed on cancer biopsies of deparaffinized Human hepatocarcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Anti-Calnexin (MA3-027) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

product information

Product Type :

Antibody

Product Name :

Calnexin Monoclonal Antibody (AF18)

Catalog # :

MA3-027

Quantity :

100 µL

Price :

US 399.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

Immunocytochemistry: 1:50-1:500, Immunohistochemistry: 1:20, Immunoprecipitation: 5 µg, RNA Immunoprecipitation: Assay-dependent, Western Blot: 1:100-1:1,000

Species :

Human, Mouse

Clone :

AF18

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Calnexin, also referred to as IP90, p88 and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).

Immunogen :

Focus human hepatoma cell lysate.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:50-1:500, Immunohistochemistry: 1:20, Immunoprecipitation: 5 µg, RNA Immunoprecipitation: Assay-dependent, Western Blot: 1:100-1:1,000

Aliases :

1110069N15Rik; AI988026; calmegin; calnexin; canx; canx-a; canx-b; clgn; clnx; cnx; D11Ertd153e; FLJ26570; hypothetical protein; IP90; Major histocompatibility complex class I antigen-binding protein p88; p88; P90; PP90; pp90 precursor

more info or order :

Invitrogen product webpage