NFATC1 Monoclonal Antibody (7A6) | Invitrogen MA3-024 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

NFATC1 Monoclonal Antibody (7A6)

catalog :

MA3-024

quantity :

100 µL

price :

US 412.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7A6

reactivity :

human, mouse, rat, domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 82

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...	Huang J, Hu M, Niu H, Wang J, Si Y, Cheng S, et al. Osteopontin isoform c promotes the survival of cisplatin-treated NSCLC cells involving NFATc2-mediated suppression on calcium-induced ROS levels. BMC Cancer. 2021;21:750 pubmed publisher
immunocytochemistry; mouse; loading ...; fig 4a western blot; mouse; loading ...; fig 4c immunocytochemistry; human; loading ...; fig 4e western blot; human; loading ...; fig 4g	Polavaram N, Dutta S, Islam R, Bag A, Roy S, Poitz D, et al. Tumor- and osteoclast-derived NRP2 in prostate cancer bone metastases. Bone Res. 2021;9:24 pubmed publisher
immunohistochemistry; mouse; 1:100; loading ...	Moore K, Fulmer D, Guo L, Koren N, Glover J, Moore R, et al. PDGFRα: Expression and Function during Mitral Valve Morphogenesis. J Cardiovasc Dev Dis. 2021;8: pubmed publisher
western blot; mouse; loading ...; fig 5a	Fajardo V, Gamu D, Mitchell A, Bloemberg D, Bombardier E, Chambers P, et al. Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice. PLoS ONE. 2017;12:e0173708 pubmed publisher
chromatin immunoprecipitation; mouse western blot; mouse; fig 5d	Hui X, Zhang M, Gu P, Li K, Gao Y, Wu D, et al. Adipocyte SIRT1 controls systemic insulin sensitivity by modulating macrophages in adipose tissue. EMBO Rep. 2017;18:645-657 pubmed publisher
western blot; human; loading ...; fig 1a	Liu H, Wang K, Chen S, Sun Q, Zhang Y, Chen L, et al. NFATc1 phosphorylation by DYRK1A increases its protein stability. PLoS ONE. 2017;12:e0172985 pubmed publisher
western blot; mouse; loading ...; fig s6b	Keller M, Paul P, Rabaglia M, Stapleton D, Schueler K, Broman A, et al. The Transcription Factor Nfatc2 Regulates β-Cell Proliferation and Genes Associated with Type 2 Diabetes in Mouse and Human Islets. PLoS Genet. 2016;12:e1006466 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:100; fig 4 western blot; mouse; 1:1000; fig 4	Liu W, Zhou L, Zhou C, Zhang S, Jing J, Xie L, et al. GDF11 decreases bone mass by stimulating osteoclastogenesis and inhibiting osteoblast differentiation. Nat Commun. 2016;7:12794 pubmed publisher
western blot; mouse; 1:2000; fig s6	Fajardo V, Smith I, Bombardier E, Chambers P, Quadrilatero J, Tupling A. Diaphragm assessment in mice overexpressing phospholamban in slow-twitch type I muscle fibers. Brain Behav. 2016;6:e00470 pubmed publisher
chromatin immunoprecipitation; mouse; fig 4	Hajibeigi A, Dioum E, Guo J, Ã–z O. Identification of novel regulatory NFAT and TFII-I binding elements in the calbindin-D28k promoter in response to serum deprivation. Biochem Biophys Res Commun. 2015;465:414-420 pubmed publisher
western blot; mouse	Alam M, Gaida M, Ogawa Y, Kolios A, Lasitschka F, Ashwell J. Counter-regulation of T cell effector function by differentially activated p38. J Exp Med. 2014;211:1257-70 pubmed publisher
immunocytochemistry; human western blot; human	Kundumani Sridharan V, Singh N, Kumar S, Gadepalli R, Rao G. Nuclear factor of activated T cells c1 mediates p21-activated kinase 1 activation in the modulation of chemokine-induced human aortic smooth muscle cell F-actin stress fiber formation, migration, and proliferation and injury-induced vascular wall rem. J Biol Chem. 2013;288:22150-62 pubmed publisher
immunocytochemistry; mouse	Tourneur E, Ben Mkaddem S, Chassin C, Bens M, Goujon J, Charles N, et al. Cyclosporine A impairs nucleotide binding oligomerization domain (Nod1)-mediated innate antibacterial renal defenses in mice and human transplant recipients. PLoS Pathog. 2013;9:e1003152 pubmed publisher
western blot; mouse; 1:2000	Salbach J, Kliemt S, Rauner M, Rachner T, Goettsch C, Kalkhof S, et al. The effect of the degree of sulfation of glycosaminoglycans on osteoclast function and signaling pathways. Biomaterials. 2012;33:8418-29 pubmed publisher
EMSA; mouse	Hermann Kleiter N, Meisel M, Fresser F, Thuille N, Muller M, Roth L, et al. Nuclear orphan receptor NR2F6 directly antagonizes NFAT and ROR?t binding to the Il17a promoter. J Autoimmun. 2012;39:428-40 pubmed publisher
immunocytochemistry; human western blot; human	Singh N, Kundumani Sridharan V, Kumar S, Verma S, Kotla S, Mukai H, et al. Protein kinase N1 is a novel substrate of NFATc1-mediated cyclin D1-CDK6 activity and modulates vascular smooth muscle cell division and migration leading to inward blood vessel wall remodeling. J Biol Chem. 2012;287:36291-304 pubmed publisher
western blot; mouse	Li T, FINCH E, Graham V, Zhang Z, Ding J, Burch J, et al. STIM1-Ca(2+) signaling is required for the hypertrophic growth of skeletal muscle in mice. Mol Cell Biol. 2012;32:3009-17 pubmed publisher
chromatin immunoprecipitation; rat	Kundumani Sridharan V, Van Quyen D, Subramani J, Singh N, Chin Y, Rao G. Novel interactions between NFATc1 (Nuclear Factor of Activated T cells c1) and STAT-3 (Signal Transducer and Activator of Transcription-3) mediate G protein-coupled receptor agonist, thrombin-induced biphasic expression of cyclin D1, with first phase. J Biol Chem. 2012;287:22463-82 pubmed publisher
chromatin immunoprecipitation; mouse western blot; mouse	Li Q, Shakya A, Guo X, Zhang H, Tantin D, Jensen P, et al. Constitutive nuclear localization of NFAT in Foxp3+ regulatory T cells independent of calcineurin activity. J Immunol. 2012;188:4268-77 pubmed publisher
western blot; mouse	Kinugawa S, Koide M, Kobayashi Y, Mizoguchi T, Ninomiya T, Muto A, et al. Tetracyclines convert the osteoclastic-differentiation pathway of progenitor cells to produce dendritic cell-like cells. J Immunol. 2012;188:1772-81 pubmed publisher
EMSA; human	Wang Q, Zhou Y, Weiss H, Chow C, Evers B. NFATc1 regulation of TRAIL expression in human intestinal cells. PLoS ONE. 2011;6:e19882 pubmed publisher
EMSA; human western blot; human	Balkan W, Rodríguez Gonzalez M, Pang M, Fernandez I, Troen B. Retinoic acid inhibits NFATc1 expression and osteoclast differentiation. J Bone Miner Metab. 2011;29:652-61 pubmed publisher
western blot; human	Wang Q, Zhou Y, Jackson L, Johnson S, Chow C, Evers B. Nuclear factor of activated T cells (NFAT) signaling regulates PTEN expression and intestinal cell differentiation. Mol Biol Cell. 2011;22:412-20 pubmed publisher
immunoprecipitation; human	Chan M, Weisman A, Kang H, Nguyen P, Hickman T, Mecker S, et al. The amiloride derivative phenamil attenuates pulmonary vascular remodeling by activating NFAT and the bone morphogenetic protein signaling pathway. Mol Cell Biol. 2011;31:517-30 pubmed publisher
immunoprecipitation; mouse	Oh M, Dey A, Gerard R, Hill J, Rothermel B. The CCAAT/enhancer binding protein beta (C/EBPbeta) cooperates with NFAT to control expression of the calcineurin regulatory protein RCAN1-4. J Biol Chem. 2010;285:16623-31 pubmed publisher
western blot; human	Karpurapu M, Wang D, Van Quyen D, Kim T, Kundumani Sridharan V, Pulusani S, et al. Cyclin D1 is a bona fide target gene of NFATc1 and is sufficient in the mediation of injury-induced vascular wall remodeling. J Biol Chem. 2010;285:3510-23 pubmed publisher
western blot; rat; 1:500	Chow W, Hou G, Bendeck M. Glycogen synthase kinase 3beta regulation of nuclear factor of activated T-cells isoform c1 in the vascular smooth muscle cell response to injury. Exp Cell Res. 2008;314:2919-29 pubmed publisher
western blot; mouse	Matza D, Badou A, Kobayashi K, Goldsmith Pestana K, Masuda Y, Komuro A, et al. A scaffold protein, AHNAK1, is required for calcium signaling during T cell activation. Immunity. 2008;28:64-74 pubmed publisher
western blot; rat	Nakazato K, Song H. Increased oxidative properties of gastrocnemius in rats fed on a high-protein diet. J Nutr Biochem. 2008;19:26-32 pubmed
western blot; mouse	Miyazaki M, Hitomi Y, Kizaki T, Ohno H, Katsumura T, Haga S, et al. Calcineurin-mediated slow-type fiber expression and growth in reloading condition. Med Sci Sports Exerc. 2006;38:1065-72 pubmed
western blot; mouse	Pfeifhofer C, Gruber T, Letschka T, Thuille N, Lutz Nicoladoni C, Hermann Kleiter N, et al. Defective IgG2a/2b class switching in PKC alpha-/- mice. J Immunol. 2006;176:6004-11 pubmed
immunocytochemistry; domestic rabbit; 1:1000 western blot; domestic rabbit; 1:1000	Larrieu D, Thiebaud P, Duplaa C, Sibon I, Theze N, Lamazière J. Activation of the Ca(2+)/calcineurin/NFAT2 pathway controls smooth muscle cell differentiation. Exp Cell Res. 2005;310:166-75 pubmed
western blot; mouse	Kaplan B, Ouyang Y, Rockwell C, Rao G, Kaminski N. 2-Arachidonoyl-glycerol suppresses interferon-gamma production in phorbol ester/ionomycin-activated mouse splenocytes independent of CB1 or CB2. J Leukoc Biol. 2005;77:966-74 pubmed
western blot; rat	De Arcangelis V, Coletti D, Canato M, Molinaro M, Adamo S, Reggiani C, et al. Hypertrophy and transcriptional regulation induced in myogenic cell line L6-C5 by an increase of extracellular calcium. J Cell Physiol. 2005;202:787-95 pubmed
	Lin Q, Zhang Y, Bai J, Liu J, Li H. VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis. Clin Transl Med. 2021;11:e374 pubmed publisher
	Sheng H, Lao Y, Zhang S, Ding W, Lu D, Xu B. Combined Pharmacotherapy with Alendronate and Desferoxamine Regulate the Bone Resorption and Bone Regeneration for Preventing Glucocorticoids-Induced Osteonecrosis of the Femoral Head. Biomed Res Int. 2020;2020:3120458 pubmed publisher
	Govatati S, Pichavaram P, Janjanam J, Guo L, Virmani R, Rao G. Myristoylation of LMCD1 Leads to Its Species-Specific Derepression of E2F1 and NFATc1 in the Modulation of CDC6 and IL-33 Expression During Development of Vascular Lesions. Arterioscler Thromb Vasc Biol. 2020;40:1256-1274 pubmed publisher
	Igari K, Kelly M, Yamanouchi D. Cigarette Smoke Extract Activates Tartrate-Resistant Acid Phosphatase-Positive Macrophage. J Vasc Res. 2019;:1-13 pubmed publisher
	Pujol J, Heikkila E, Savoia C, Hajibeigi A, De Marchi U, Battiprolu P, et al. Isx9 Regulates Calbindin D28K Expression in Pancreatic β Cells and Promotes β Cell Survival and Function. Int J Mol Sci. 2018;19: pubmed publisher
	Fujita M, Yagi T, Okura U, Tanaka J, Hirashima N, Tanaka M. Calcineurin B1 Deficiency in Glial Cells Induces Mucosal Degeneration and Inflammation in Mouse Small Intestine. Biol Pharm Bull. 2018;41:786-796 pubmed publisher
	Kotla S, Singh N, Kirchhofer D, Rao G. Heterodimers of the transcriptional factors NFATc3 and FosB mediate tissue factor expression for 15(S)-hydroxyeicosatetraenoic acid-induced monocyte trafficking. J Biol Chem. 2017;292:14885-14901 pubmed publisher
	Singh N, Janjanam J, Rao G. p115 RhoGEF activates the Rac1 GTPase signaling cascade in MCP1 chemokine-induced vascular smooth muscle cell migration and proliferation. J Biol Chem. 2017;292:14080-14091 pubmed publisher
	Fajardo V, Rietze B, Chambers P, Bellissimo C, Bombardier E, Quadrilatero J, et al. Effects of sarcolipin deletion on skeletal muscle adaptive responses to functional overload and unload. Am J Physiol Cell Physiol. 2017;313:C154-C161 pubmed publisher
	Tang Y, Abe N, Yoshimoto M, Zhu B, Murata Y, Nakamura Y. Benzyl isothiocyanate inhibits IL-13 expression in human basophilic KU812 cells. Biosci Biotechnol Biochem. 2015;79:159-63 pubmed publisher
	Torgerson T, Genin A, Chen C, Zhang M, Zhou B, Anover Sombke S, et al. FOXP3 inhibits activation-induced NFAT2 expression in T cells thereby limiting effector cytokine expression. J Immunol. 2009;183:907-15 pubmed publisher
	Karpurapu M, Wang D, Singh N, Li Q, Rao G. NFATc1 targets cyclin A in the regulation of vascular smooth muscle cell multiplication during restenosis. J Biol Chem. 2008;283:26577-90 pubmed publisher
	Wu W, Misra R, Russell J, Flavell R, Rincon M, Budd R. Proteolytic regulation of nuclear factor of activated T (NFAT) c2 cells and NFAT activity by caspase-3. J Biol Chem. 2006;281:10682-90 pubmed
	Shen T, Liu Y, Cseresnyes Z, Hawkins A, Randall W, Schneider M. Activity- and calcineurin-independent nuclear shuttling of NFATc1, but not NFATc3, in adult skeletal muscle fibers. Mol Biol Cell. 2006;17:1570-82 pubmed
	Scicchitano B, Spath L, Musaro A, Molinaro M, Rosenthal N, Nervi C, et al. Vasopressin-dependent myogenic cell differentiation is mediated by both Ca2+/calmodulin-dependent kinase and calcineurin pathways. Mol Biol Cell. 2005;16:3632-41 pubmed
	Kim H, Korn L, Gamero A, Leonard W. Calcium-dependent activation of interleukin-21 gene expression in T cells. J Biol Chem. 2005;280:25291-7 pubmed
	Liu Z, Zhang C, Dronadula N, Li Q, Rao G. Blockade of nuclear factor of activated T cells activation signaling suppresses balloon injury-induced neointima formation in a rat carotid artery model. J Biol Chem. 2005;280:14700-8 pubmed
	Miyazaki M, Hitomi Y, Kizaki T, Ohno H, Haga S, Takemasa T. Contribution of the calcineurin signaling pathway to overload-induced skeletal muscle fiber-type transition. J Physiol Pharmacol. 2004;55:751-64 pubmed
	Minami T, Horiuchi K, Miura M, Abid M, Takabe W, Noguchi N, et al. Vascular endothelial growth factor- and thrombin-induced termination factor, Down syndrome critical region-1, attenuates endothelial cell proliferation and angiogenesis. J Biol Chem. 2004;279:50537-54 pubmed
	Barlic J, McDermott D, Merrell M, Gonzales J, Via L, Murphy P. Interleukin (IL)-15 and IL-2 reciprocally regulate expression of the chemokine receptor CX3CR1 through selective NFAT1- and NFAT2-dependent mechanisms. J Biol Chem. 2004;279:48520-34 pubmed
	Cannon J, Burkhardt J. Differential roles for Wiskott-Aldrich syndrome protein in immune synapse formation and IL-2 production. J Immunol. 2004;173:1658-62 pubmed
	McCullagh K, Calabria E, Pallafacchina G, Ciciliot S, Serrano A, Argentini C, et al. NFAT is a nerve activity sensor in skeletal muscle and controls activity-dependent myosin switching. Proc Natl Acad Sci U S A. 2004;101:10590-5 pubmed
	Frazer Abel A, Baksh S, Fosmire S, Willis D, Pierce A, Meylemans H, et al. Nicotine activates nuclear factor of activated T cells c2 (NFATc2) and prevents cell cycle entry in T cells. J Pharmacol Exp Ther. 2004;311:758-69 pubmed
	Monticelli S, Solymar D, Rao A. Role of NFAT proteins in IL13 gene transcription in mast cells. J Biol Chem. 2004;279:36210-8 pubmed
	Rosenberg P, Hawkins A, Stiber J, Shelton J, Hutcheson K, Bassel Duby R, et al. TRPC3 channels confer cellular memory of recent neuromuscular activity. Proc Natl Acad Sci U S A. 2004;101:9387-92 pubmed
	Pfeifhofer C, Kofler K, Gruber T, Tabrizi N, Lutz C, Maly K, et al. Protein kinase C theta affects Ca2+ mobilization and NFAT cell activation in primary mouse T cells. J Exp Med. 2003;197:1525-35 pubmed
	Yellaturu C, Ghosh S, Rao R, Jennings L, Hassid A, Rao G. A potential role for nuclear factor of activated T-cells in receptor tyrosine kinase and G-protein-coupled receptor agonist-induced cell proliferation. Biochem J. 2002;368:183-90 pubmed
	Diehl S, Chow C, Weiss L, Palmetshofer A, Twardzik T, Rounds L, et al. Induction of NFATc2 expression by interleukin 6 promotes T helper type 2 differentiation. J Exp Med. 2002;196:39-49 pubmed
	Kim H, Leonard W. The basis for TCR-mediated regulation of the IL-2 receptor alpha chain gene: role of widely separated regulatory elements. EMBO J. 2002;21:3051-9 pubmed
	Dupont Versteegden E, Knox M, Gurley C, Houle J, Peterson C. Maintenance of muscle mass is not dependent on the calcineurin-NFAT pathway. Am J Physiol Cell Physiol. 2002;282:C1387-95 pubmed
	Jan T, Rao G, Kaminski N. Cannabinol enhancement of interleukin-2 (IL-2) expression by T cells is associated with an increase in IL-2 distal nuclear factor of activated T cell activity. Mol Pharmacol. 2002;61:446-54 pubmed
	Kehoe K, Brown M, Imani F. Double-stranded RNA regulates IL-4 expression. J Immunol. 2001;167:2496-501 pubmed
	Mukerjee N, McGinnis K, Gnegy M, Wang K. Caspase-mediated calcineurin activation contributes to IL-2 release during T cell activation. Biochem Biophys Res Commun. 2001;285:1192-9 pubmed
	Keen J, Sholl L, Wills Karp M, Georas S. Preferential activation of nuclear factor of activated T cells c correlates with mouse strain susceptibility to allergic responses and interleukin-4 gene expression. Am J Respir Cell Mol Biol. 2001;24:58-65 pubmed
	Swoap S, Hunter R, Stevenson E, Felton H, Kansagra N, Lang J, et al. The calcineurin-NFAT pathway and muscle fiber-type gene expression. Am J Physiol Cell Physiol. 2000;279:C915-24 pubmed
	Chow C, Dong C, Flavell R, Davis R. c-Jun NH(2)-terminal kinase inhibits targeting of the protein phosphatase calcineurin to NFATc1. Mol Cell Biol. 2000;20:5227-34 pubmed
	Chen R, Burke T, Cumberland J, Brummet M, Beck L, Casolaro V, et al. Glucocorticoids inhibit calcium- and calcineurin-dependent activation of the human IL-4 promoter. J Immunol. 2000;164:825-32 pubmed
	Wierenga E, Walchner M, Kick G, Kapsenberg M, Weiss E, Messer G. Evidence for suppressed activity of the transcription factor NFAT1 at its proximal binding element P0 in the IL-4 promoter associated with enhanced IL-4 gene transcription in T cells of atopic patients. Int Immunol. 1999;11:297-306 pubmed
	Li Weber M, Giasi M, Krammer P. Involvement of Jun and Rel proteins in up-regulation of interleukin-4 gene activity by the T cell accessory molecule CD28. J Biol Chem. 1998;273:32460-6 pubmed
	Abbott K, Friday B, Thaloor D, Murphy T, Pavlath G. Activation and cellular localization of the cyclosporine A-sensitive transcription factor NF-AT in skeletal muscle cells. Mol Biol Cell. 1998;9:2905-16 pubmed
	Boss V, Wang X, Koppelman L, Xu K, Murphy T. Histamine induces nuclear factor of activated T cell-mediated transcription and cyclosporin A-sensitive interleukin-8 mRNA expression in human umbilical vein endothelial cells. Mol Pharmacol. 1998;54:264-72 pubmed
	Li Weber M, Salgame P, Hu C, Davydov I, Laur O, Klevenz S, et al. Th2-specific protein/DNA interactions at the proximal nuclear factor-AT site contribute to the functional activity of the human IL-4 promoter. J Immunol. 1998;161:1380-9 pubmed
	Boss V, Abbott K, Wang X, Pavlath G, Murphy T. The cyclosporin A-sensitive nuclear factor of activated T cells (NFAT) proteins are expressed in vascular smooth muscle cells. Differential localization of NFAT isoforms and induction of NFAT-mediated transcription by phospholipase C-coupled cell sur. J Biol Chem. 1998;273:19664-71 pubmed
	Latinis K, Norian L, Eliason S, Koretzky G. Two NFAT transcription factor binding sites participate in the regulation of CD95 (Fas) ligand expression in activated human T cells. J Biol Chem. 1997;272:31427-34 pubmed
	Chow C, Rincon M, Cavanagh J, Dickens M, Davis R. Nuclear accumulation of NFAT4 opposed by the JNK signal transduction pathway. Science. 1997;278:1638-41 pubmed
	Lyakh L, Ghosh P, Rice N. Expression of NFAT-family proteins in normal human T cells. Mol Cell Biol. 1997;17:2475-84 pubmed
	Northrop J, Ho S, Chen L, Thomas D, Timmerman L, Nolan G, et al. NF-AT components define a family of transcription factors targeted in T-cell activation. Nature. 1994;369:497-502 pubmed
	Ho S, Thomas D, Timmerman L, Li X, Francke U, Crabtree G. NFATc3, a lymphoid-specific NFATc family member that is calcium-regulated and exhibits distinct DNA binding specificity. J Biol Chem. 1995;270:19898-907 pubmed

image

image 1 :

Immunofluorescent analysis of NFATc1 using NFATc1 Monoclonal Antibody (7A6) (Product# MA3-024 ) shows staining in 293Cells. NFATc1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc1 (Product# MA3-024 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

image 2 :

Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing NFATc1 (MA3-024) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

product information

Product Type :

Antibody

Product Name :

NFATC1 Monoclonal Antibody (7A6)

Catalog # :

MA3-024

Quantity :

100 µL

Price :

US 412.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human, Mouse, Non-human primate, Rat

Applications :

ChIP assay: Assay-dependent, Gel Shift: Assay-dependent, Immunocytochemistry: 1:250, Immunohistochemistry (Paraffin): Assay-dependent, Immunohistochemistry (PFA fixed): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:2,000

Species :

Human, Mouse, Non-human primate, Rat

Clone :

7A6

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.

Immunogen :

Bacterially expressed glutathione S-transferase (GST) fusion protein containing NF-ATc1 residues 1-654.

Format :

Liquid

Applications w/Dilutions :

Aliases :

2210017P03Rik; AI449492; AV076380; MGC138448; NFAT tra; NFAT trans; NFAT transcription complex cytosolic component; NFAT2; NFATc; NF-ATC; NFATC1; NF-ATc1; NF-ATc1.2; Nfatcb; nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1; nuclear factor of activated T-cells 1; nuclear factor of activated T-cells 'c'; nuclear factor of activated T-cells, cytoplasmic 1; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1; transcription factor NF-ATc

more info or order :

Invitrogen product webpage