HSP60 Monoclonal Antibody (A57-B9) | Invitrogen MA3-023 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HSP60 Monoclonal Antibody (A57-B9)

catalog :

MA3-023

quantity :

100 µL

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A57-B9

reactivity :

Chlamydia pneumoniae, Chlamydia trachomatis, human, pigs , Chlamydia trachomatis D/UW-3/CX

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
western blot; Chlamydia trachomatis; fig 3b	Parrett C, Lenoci R, Nguyen B, Russell L, Jewett T. Targeted Disruption of Chlamydia trachomatis Invasion by in Trans Expression of Dominant Negative Tarp Effectors. Front Cell Infect Microbiol. 2016;6:84 pubmed publisher
immunocytochemistry; Chlamydia trachomatis; fig 3	Messinger J, Nelton E, Feeney C, Gondek D. Chlamydia Infection Across Host Species Boundaries Promotes Distinct Sets of Transcribed Anti-Apoptotic Factors. Front Cell Infect Microbiol. 2015;5:96 pubmed publisher
immunocytochemistry; human	Boncompain G, Müller C, Meas Yedid V, Schmitt Kopplin P, Lazarow P, Subtil A. The intracellular bacteria Chlamydia hijack peroxisomes and utilize their enzymatic capacity to produce bacteria-specific phospholipids. PLoS ONE. 2014;9:e86196 pubmed publisher
immunohistochemistry; Chlamydia trachomatis	Atik B, Johnston S, Dean D. Association of carotid plaque Lp-PLA(2) with macrophages and Chlamydia pneumoniae infection among patients at risk for stroke. PLoS ONE. 2010;5:e11026 pubmed publisher
western blot; pigs	Deniset J, Cheung P, Dibrov E, Lee K, Steigerwald S, Pierce G. Chlamydophila pneumoniae infection leads to smooth muscle cell proliferation and thickening in the coronary artery without contributions from a host immune response. Am J Pathol. 2010;176:1028-37 pubmed publisher
immunohistochemistry; Chlamydia pneumoniae	Arno G, Kaski J, Smith D, Akiyu J, Hughes S, Baboonian C. Matrix metalloproteinase-9 expression is associated with the presence of Chlamydia pneumoniae in human coronary atherosclerotic plaques. Heart. 2005;91:521-5 pubmed
	Cossé M, Barta M, Fisher D, Oesterlin L, Niragire B, Perrinet S, et al. The Loss of Expression of a Single Type 3 Effector (CT622) Strongly Reduces Chlamydia trachomatis Infectivity and Growth. Front Cell Infect Microbiol. 2018;8:145 pubmed publisher
	Gieffers J, Füllgraf H, Jahn J, Klinger M, Dalhoff K, Katus H, et al. Chlamydia pneumoniae infection in circulating human monocytes is refractory to antibiotic treatment. Circulation. 2001;103:351-6 pubmed
	Meijer A, van der Vliet J, Roholl P, Gielis Proper S, de Vries A, Ossewaarde J. Chlamydia pneumoniae in abdominal aortic aneurysms: abundance of membrane components in the absence of heat shock protein 60 and DNA. Arterioscler Thromb Vasc Biol. 1999;19:2680-6 pubmed
	Beatty W, Byrne G, Morrison R. Morphologic and antigenic characterization of interferon gamma-mediated persistent Chlamydia trachomatis infection in vitro. Proc Natl Acad Sci U S A. 1993;90:3998-4002 pubmed
	Beatty W, Belanger T, Desai A, Morrison R, Byrne G. Tryptophan depletion as a mechanism of gamma interferon-mediated chlamydial persistence. Infect Immun. 1994;62:3705-11 pubmed
	Beatty W, Morrison R, Byrne G. Reactivation of persistent Chlamydia trachomatis infection in cell culture. Infect Immun. 1995;63:199-205 pubmed
	Yuan Y, Lyng K, Zhang Y, Rockey D, Morrison R. Monoclonal antibodies define genus-specific, species-specific, and cross-reactive epitopes of the chlamydial 60-kilodalton heat shock protein (hsp60): specific immunodetection and purification of chlamydial hsp60. Infect Immun. 1992;60:2288-96 pubmed

image

image 1 :

Immunofluorescent analysis of Heat Shock Protein 60 using Heat Shock Protein 60 Monoclonal Antibody (A57-B9) (Product# MA3-023 ) shows staining in 293Cells. Heat Shock Protein 60 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Heat Shock Protein 60 (Product# MA3-023 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

image 2 :

Immunofluorescent analysis of Heat Shock Protein 60 using Heat Shock Protein 60 Monoclonal Antibody (A57-B9) (Product# MA3-023 ) shows staining in BEAS-2B Cells. Heat Shock Protein 60 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Heat Shock Protein 60 (Product# MA3-023 ) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

image 3 :

Immunofluorescent analysis of Heat Shock Protein 60 using Heat Shock Protein 60 Monoclonal Antibody (A57-B9) (Product# MA3-023 ) shows staining in Hela Cells. Heat Shock Protein 60 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Heat Shock Protein 60 (Product# MA3-023 ) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

HSP60 Monoclonal Antibody (A57-B9)

Catalog # :

MA3-023

Quantity :

100 µL

Price :

US 399.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Bacteria

Applications :

Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Species :

Bacteria

Clone :

A57-B9

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.

Immunogen :

Recombinant Chlamydia trachomatis HSP60 protein.

Format :

Liquid

Applications w/Dilutions :

Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Aliases :

60 kDa chaperonin; 60 kDa heat shock protein; 60 kDa heat shock protein, mitochondrial; 60kDa; Chaperonin 60; CPN60; GROEL; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1; heat shock 60kDa protein 1 (chaperonin); Heat shock protein; heat shock protein 1 (chaperonin); heat shock protein 60; heat shock protein 60 (liver); heat shock protein 65; heat shock protein family D (Hsp60) member 1; heat shock protein, 60 kDa; HLD4; HSP; Hsp60; HSP-60; Hsp60s1; HSP65; HSP-65; Hspd1; Hspd1-30p; HuCHA60; hypothetical protein; I79_011398; mitochondrial matrix protein P1; P1 protein; P60 lymphocyte protein; RCJMB04_7g5; short heat shock protein 60 Hsp60s1; SPG13; Unknown (protein for IMAGE:7943409)

more info or order :

Invitrogen product webpage