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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
PDI Monoclonal Antibody (RL90)
catalog :
MA3-019
quantity :
100 µL
price :
US 425.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
RL90
reactivity :
Chinese hamsters, African green monkey, human, mouse, rat, pigs , rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments
more info or order :
Invitrogen product webpage
citations: 80
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; loading ...; fig 7c
Haag A, Walser M, Henggeler A, Hajnal A. The CHORD protein CHP-1 regulates EGF receptor trafficking and signaling in C. elegans and in human cells. elife. 2020;9: pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 6a
Fukushima M, Dasgupta D, Mauer A, Kakazu E, Nakao K, Malhi H. StAR-related lipid transfer domain 11 (STARD11)-mediated ceramide transport mediates extracellular vesicle biogenesis. J Biol Chem. 2018;293:15277-15289 pubmed publisher
  • western blot; mouse; loading ...; fig 6a
Moretti A, Pavanelli J, Nolasco P, Leisegang M, Tanaka L, Fernandes C, et al. Conserved Gene Microsynteny Unveils Functional Interaction Between Protein Disulfide Isomerase and Rho Guanine-Dissociation Inhibitor Families. Sci Rep. 2017;7:17262 pubmed publisher
  • immunocytochemistry; human; 1:300; loading ...; fig 6b
Waters A, Ozkan Dagliyan I, Vaseva A, Fer N, Strathern L, Hobbs G, et al. Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies. Sci Signal. 2017;10: pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig 4a
Yamashita Y, Anczurowski M, Nakatsugawa M, Tanaka M, Kagoya Y, Sinha A, et al. HLA-DP84Gly constitutively presents endogenous peptides generated by the class I antigen processing pathway. Nat Commun. 2017;8:15244 pubmed publisher
  • western blot; rat; loading ...; fig 2c
Wang H, Tri Anggraini F, Chen X, DeGracia D. Embryonic lethal abnormal vision proteins and adenine and uridine-rich element mRNAs after global cerebral ischemia and reperfusion in the rat. J Cereb Blood Flow Metab. 2017;37:1494-1507 pubmed publisher
  • immunocytochemistry; human; 50 ug/ml; fig 4
Pacello F, D Orazio M, Battistoni A. An ERp57-mediated disulphide exchange promotes the interaction between Burkholderia cenocepacia and epithelial respiratory cells. Sci Rep. 2016;6:21140 pubmed publisher
  • immunocytochemistry; human; fig s9
Rost B, Schneider F, Grauel M, Wozny C, Bentz C, Blessing A, et al. Optogenetic acidification of synaptic vesicles and lysosomes. Nat Neurosci. 2015;18:1845-1852 pubmed publisher
  • immunocytochemistry; rat
Cheng D, Weckerle A, Yu Y, Ma L, Zhu X, Murea M, et al. Biogenesis and cytotoxicity of APOL1 renal risk variant proteins in hepatocytes and hepatoma cells. J Lipid Res. 2015;56:1583-93 pubmed publisher
  • western blot; human; 1:2000; fig 1b
Janes K. An analysis of critical factors for quantitative immunoblotting. Sci Signal. 2015;8:rs2 pubmed publisher
  • immunocytochemistry; rhesus macaque; fig 2
van Bergeijk P, Adrian M, Hoogenraad C, Kapitein L. Optogenetic control of organelle transport and positioning. Nature. 2015;518:111-114 pubmed publisher
  • western blot; human; fig 1,2,3,4,5,6
Cherepanova N, Shrimal S, Gilmore R. Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins. J Cell Biol. 2014;206:525-39 pubmed publisher
  • western blot; human
Saito R, Kaneko M, Kitamura Y, Takata K, Kawada K, Okuma Y, et al. Effects of oxidative stress on the solubility of HRD1, a ubiquitin ligase implicated in Alzheimer's disease. PLoS ONE. 2014;9:e94576 pubmed publisher
  • western blot; rat; 1:20000, 1:100000
Wlcek K, Hofstetter L, Stieger B. Transport of estradiol-17?-glucuronide, estrone-3-sulfate and taurocholate across the endoplasmic reticulum membrane: evidence for different transport systems. Biochem Pharmacol. 2014;88:106-18 pubmed publisher
  • immunocytochemistry; human
Matsumoto M, Katsuyama M, Iwata K, Ibi M, Zhang J, Zhu K, et al. Characterization of N-glycosylation sites on the extracellular domain of NOX1/NADPH oxidase. Free Radic Biol Med. 2014;68:196-204 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:200
  • immunohistochemistry; rat; 1:200
Lewis M, Jamison J, Dunbar J, DeGracia D. mRNA redistribution during permanent focal cerebral ischemia. Transl Stroke Res. 2013;4:604-17 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Numata Y, Morimura T, Nakamura S, Hirano E, Kure S, Goto Y, et al. Depletion of molecular chaperones from the endoplasmic reticulum and fragmentation of the Golgi apparatus associated with pathogenesis in Pelizaeus-Merzbacher disease. J Biol Chem. 2013;288:7451-66 pubmed publisher
  • flow cytometry; rhesus macaque
Calderon M, Guerrero C, Acosta O, Lopez S, Arias C. Inhibiting rotavirus infection by membrane-impermeant thiol/disulfide exchange blockers and antibodies against protein disulfide isomerase. Intervirology. 2012;55:451-64 pubmed publisher
  • western blot; human
Buschow S, Lasonder E, Szklarczyk R, Oud M, de Vries I, Figdor C. Unraveling the human dendritic cell phagosome proteome by organellar enrichment ranking. J Proteomics. 2012;75:1547-62 pubmed publisher
  • immunocytochemistry; human; 1:100
Messaed C, Akoury E, Djuric U, Zeng J, Saleh M, Gilbert L, et al. NLRP7, a nucleotide oligomerization domain-like receptor protein, is required for normal cytokine secretion and co-localizes with Golgi and the microtubule-organizing center. J Biol Chem. 2011;286:43313-23 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:200
  • immunohistochemistry; rat; 1:200
  • western blot; rat; 1:1000
Jamison J, Szymanski J, DeGracia D. Organelles do not colocalize with mRNA granules in post-ischemic neurons. Neuroscience. 2011;199:394-400 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Matsushita E, Asai N, Enomoto A, Kawamoto Y, Kato T, Mii S, et al. Protective role of Gipie, a Girdin family protein, in endoplasmic reticulum stress responses in endothelial cells. Mol Biol Cell. 2011;22:736-47 pubmed publisher
  • immunocytochemistry; human; 1:1000
Wen X, Abe T, Kukihara H, Taguwa S, Mori Y, Tani H, et al. Elimination of hepatitis C virus from hepatocytes by a selective activation of therapeutic molecules. PLoS ONE. 2011;6:e15967 pubmed publisher
  • immunocytochemistry; rat
Nevalainen M, Kaisto T, Metsikkö K. Mobile ER-to-Golgi but not post-Golgi membrane transport carriers disappear during the terminal myogenic differentiation. Cell Tissue Res. 2010;342:107-16 pubmed publisher
  • immunocytochemistry; African green monkey; 1:100
Yuasa N, Zhang W, Goto T, Sakaue H, Matsumoto Takasaki A, Kimura M, et al. Production of anti-carbohydrate antibodies by phage display technologies: potential impairment of cell growth as a result of endogenous expression. J Biol Chem. 2010;285:30587-97 pubmed publisher
  • immunocytochemistry; mouse
Carnegie J, Robert Cooperman C, Wu J, Young R, Wolf B, Burkhardt B. Characterization of the expression, localization, and secretion of PANDER in alpha-cells. Mol Cell Endocrinol. 2010;325:36-45 pubmed publisher
  • immunoprecipitation; human; 10 ug/ml
  • western blot; human; 1 ug/ml
Swiatkowska M, Padula G, Michalec L, Stasiak M, Skurzynski S, Cierniewski C. Ero1alpha is expressed on blood platelets in association with protein-disulfide isomerase and contributes to redox-controlled remodeling of alphaIIbbeta3. J Biol Chem. 2010;285:29874-83 pubmed publisher
  • immunocytochemistry; human; 1:100
Eleveld Trancikova D, Sanecka A, van Hout Kuijer M, Looman M, Hendriks I, Jansen B, et al. DC-STAMP interacts with ER-resident transcription factor LUMAN which becomes activated during DC maturation. Mol Immunol. 2010;47:1963-73 pubmed publisher
  • immunocytochemistry; human; 1:1000
Schmidt J, Yvone G, Brown W. Membrane topology of human AGPAT3 (LPAAT3). Biochem Biophys Res Commun. 2010;397:661-7 pubmed publisher
  • western blot; human
Unsworth H, Raguz S, Edwards H, Higgins C, Yague E. mRNA escape from stress granule sequestration is dictated by localization to the endoplasmic reticulum. FASEB J. 2010;24:3370-80 pubmed publisher
  • western blot; human
Kaneko M, Koike H, Saito R, Kitamura Y, Okuma Y, Nomura Y. Loss of HRD1-mediated protein degradation causes amyloid precursor protein accumulation and amyloid-beta generation. J Neurosci. 2010;30:3924-32 pubmed publisher
  • immunohistochemistry; human
Hino S, Kondo S, Yoshinaga K, Saito A, Murakami T, Kanemoto S, et al. Regulation of ER molecular chaperone prevents bone loss in a murine model for osteoporosis. J Bone Miner Metab. 2010;28:131-8 pubmed publisher
  • immunoprecipitation; mouse
Santos C, Stolf B, Takemoto P, Amanso A, Lopes L, Souza E, et al. Protein disulfide isomerase (PDI) associates with NADPH oxidase and is required for phagocytosis of Leishmania chagasi promastigotes by macrophages. J Leukoc Biol. 2009;86:989-98 pubmed publisher
  • immunocytochemistry; human; 1:100
Pyle A, Li B, Maupin A, Guzman R, Crimmins D, Olson S, et al. Biomechanical stress induces novel arterial intima-enriched genes: implications for vascular adaptation to stress. Cardiovasc Pathol. 2010;19:e13-20 pubmed publisher
  • immunocytochemistry; human
Ludwig A, Blume J, Diep T, Yuan J, Mateos J, Leuthäuser K, et al. Calsyntenins mediate TGN exit of APP in a kinesin-1-dependent manner. Traffic. 2009;10:572-89 pubmed publisher
  • flow cytometry; human
  • immunoprecipitation; human
  • western blot; human
Swiatkowska M, Szymanski J, Padula G, Cierniewski C. Interaction and functional association of protein disulfide isomerase with alphaVbeta3 integrin on endothelial cells. FEBS J. 2008;275:1813-23 pubmed publisher
  • western blot; mouse
Gramolini A, Kislinger T, Alikhani Koopaei R, Fong V, Thompson N, Isserlin R, et al. Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses. Mol Cell Proteomics. 2008;7:519-33 pubmed
  • blocking or activating experiments; human; 1:500
  • immunoprecipitation; human
  • western blot; human
Manickam N, Sun X, Li M, Gazitt Y, Essex D. Protein disulphide isomerase in platelet function. Br J Haematol. 2008;140:223-9 pubmed
  • immunoprecipitation; human
  • immunoprecipitation; mouse
Mohler P, Zhu M, Blade A, Ham A, Shelness G, Swift L. Identification of a novel isoform of microsomal triglyceride transfer protein. J Biol Chem. 2007;282:26981-8 pubmed
  • immunohistochemistry; human; 1:300
Csaba Z, Lelouvier B, Viollet C, El Ghouzzi V, Toyama K, Videau C, et al. Activated somatostatin type 2 receptors traffic in vivo in central neurons from dendrites to the trans Golgi before recycling. Traffic. 2007;8:820-34 pubmed
  • immunohistochemistry; rat
Nissinen M, Kaisto T, Salmela P, Peltonen J, Metsikkö K. Restricted distribution of mRNAs encoding a sarcoplasmic reticulum or transverse tubule protein in skeletal myofibers. J Histochem Cytochem. 2005;53:217-27 pubmed
  • immunocytochemistry; human
Eleveld Trancikova D, Triantis V, Moulin V, Looman M, Wijers M, Fransen J, et al. The dendritic cell-derived protein DC-STAMP is highly conserved and localizes to the endoplasmic reticulum. J Leukoc Biol. 2005;77:337-43 pubmed
Chen M, Amado N, Tan J, Reis A, Ge M, Abreu J, et al. TMEM79/MATTRIN defines a pathway for Frizzled regulation and is required for Xenopus embryogenesis. elife. 2020;9: pubmed publisher
Lin H, Lo Y, Wang W, Huang K, I K, Chang G. Overexpression of FAM46A, a Non-canonical Poly(A) Polymerase, Promotes Hemin-Induced Hemoglobinization in K562 Cells. Front Cell Dev Biol. 2020;8:414 pubmed publisher
Ma Lauer Y, Zheng Y, Malesevic M, von Brunn B, Fischer G, von Brunn A. Influences of cyclosporin A and non-immunosuppressive derivatives on cellular cyclophilins and viral nucleocapsid protein during human coronavirus 229E replication. Antiviral Res. 2020;173:104620 pubmed publisher
Alenquer M, Vale Costa S, Etibor T, Ferreira F, Sousa A, Amorim M. Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites. Nat Commun. 2019;10:1629 pubmed publisher
Komura H, Kakio S, Sasahara T, Arai Y, Takino N, Sato M, et al. Alzheimer Aβ Assemblies Accumulate in Excitatory Neurons upon Proteasome Inhibition and Kill Nearby NAKα3 Neurons by Secretion. iScience. 2019;: pubmed publisher
De Bessa T, Pagano A, Moretti A, Oliveira P, Mendonça S, Kovacic H, et al. Subverted regulation of Nox1 NADPH oxidase-dependent oxidant generation by protein disulfide isomerase A1 in colon carcinoma cells with overactivated KRas. Cell Death Dis. 2019;10:143 pubmed publisher
Gao Q, Yang Z, Xu S, Li X, Yang X, Jin P, et al. Heterotypic CAF-tumor spheroids promote early peritoneal metastatis of ovarian cancer. J Exp Med. 2019;216:688-703 pubmed publisher
Araujo T, Venturini G, Moretti A, Tanaka L, Pereira A, Laurindo F. Cell-surface HSP70 associates with thrombomodulin in endothelial cells. Cell Stress Chaperones. 2019;24:273-282 pubmed publisher
Bravo Sagua R, Parra V, Ortiz Sandoval C, Navarro Marquez M, Rodriguez A, Diaz Valdivia N, et al. Caveolin-1 impairs PKA-DRP1-mediated remodelling of ER-mitochondria communication during the early phase of ER stress. Cell Death Differ. 2019;26:1195-1212 pubmed publisher
Lambert L, Dubayle D, Fafouri A, Herzog E, Csaba Z, Dournaud P, et al. Endocytosis of Activated Muscarinic m2 Receptor (m2R) in Live Mouse Hippocampal Neurons Occurs via a Clathrin-Dependent Pathway. Front Cell Neurosci. 2018;12:450 pubmed publisher
Lobet E, Willemart K, Ninane N, Demazy C, Sedzicki J, Lelubre C, et al. Mitochondrial fragmentation affects neither the sensitivity to TNFα-induced apoptosis of Brucella-infected cells nor the intracellular replication of the bacteria. Sci Rep. 2018;8:5173 pubmed publisher
Weidner J, Jarenbäck L, Åberg I, Westergren Thorsson G, Ankerst J, Bjermer L, et al. Endoplasmic reticulum, Golgi, and lysosomes are disorganized in lung fibroblasts from chronic obstructive pulmonary disease patients. Physiol Rep. 2018;6: pubmed publisher
Yi M, Melkonian A, Ousey J, Khosla C. Endoplasmic reticulum-resident protein 57 (ERp57) oxidatively inactivates human transglutaminase 2. J Biol Chem. 2018;293:2640-2649 pubmed publisher
Yen J, Wei L, Chen L, Chen L, Hung C, Wang S, et al. Subcellular Localization and Functional Characterization of GII.4 Norovirus-Encoded NTPase. J Virol. 2018;92: pubmed publisher
Janickova H, Prado V, Prado M, El Mestikawy S, Bernard V. Vesicular acetylcholine transporter (VAChT) over-expression induces major modifications of striatal cholinergic interneuron morphology and function. J Neurochem. 2017;142:857-875 pubmed publisher
Manuel A, Walla M, Faccenda A, Martin S, Tanis R, Piroli G, et al. Succination of Protein Disulfide Isomerase Links Mitochondrial Stress and Endoplasmic Reticulum Stress in the Adipocyte During Diabetes. Antioxid Redox Signal. 2017;27:1281-1296 pubmed publisher
Tanaka L, Araújo H, Hironaka G, Araujo T, Takimura C, Rodriguez A, et al. Peri/Epicellular Protein Disulfide Isomerase Sustains Vascular Lumen Caliber Through an Anticonstrictive Remodeling Effect. Hypertension. 2016;67:613-22 pubmed publisher
Yamamoto T, Shimojima K, Sangu N, Komoike Y, Ishii A, Abe S, et al. Single nucleotide variations in CLCN6 identified in patients with benign partial epilepsies in infancy and/or febrile seizures. PLoS ONE. 2015;10:e0118946 pubmed publisher
Suzuki M, Otsuka T, Ohsaki Y, Cheng J, Taniguchi T, Hashimoto H, et al. Derlin-1 and UBXD8 are engaged in dislocation and degradation of lipidated ApoB-100 at lipid droplets. Mol Biol Cell. 2012;23:800-10 pubmed publisher
Wu Y, Ahmad S, Zhou J, Wang L, Cully M, Essex D. The disulfide isomerase ERp57 mediates platelet aggregation, hemostasis, and thrombosis. Blood. 2012;119:1737-46 pubmed publisher
Lelouvier B, Tamagno G, Kaindl A, Roland A, Lelievre V, Le Verche V, et al. Dynamics of somatostatin type 2A receptor cargoes in living hippocampal neurons. J Neurosci. 2008;28:4336-49 pubmed publisher
Yamaguchi Y, Larkin D, Lara Lemus R, Ramos Castaneda J, Liu M, Arvan P. Endoplasmic reticulum (ER) chaperone regulation and survival of cells compensating for deficiency in the ER stress response kinase, PERK. J Biol Chem. 2008;283:17020-9 pubmed publisher
Blagoveshchenskaya A, Cheong F, Rohde H, Glover G, Knödler A, Nicolson T, et al. Integration of Golgi trafficking and growth factor signaling by the lipid phosphatase SAC1. J Cell Biol. 2008;180:803-12 pubmed publisher
Janiszewski M, Lopes L, Carmo A, Pedro M, Brandes R, Santos C, et al. Regulation of NAD(P)H oxidase by associated protein disulfide isomerase in vascular smooth muscle cells. J Biol Chem. 2005;280:40813-9 pubmed
Jordan P, Stevens J, Hubbard G, Barrett N, Sage T, Authi K, et al. A role for the thiol isomerase protein ERP5 in platelet function. Blood. 2005;105:1500-7 pubmed
Mizrachi D, Segaloff D. Intracellularly located misfolded glycoprotein hormone receptors associate with different chaperone proteins than their cognate wild-type receptors. Mol Endocrinol. 2004;18:1768-77 pubmed
Flores Díaz M, Higuita J, Florin I, Okada T, Pollesello P, Bergman T, et al. A cellular UDP-glucose deficiency causes overexpression of glucose/oxygen-regulated proteins independent of the endoplasmic reticulum stress elements. J Biol Chem. 2004;279:21724-31 pubmed
Markovic I, Stantchev T, Fields K, Tiffany L, Tomic M, Weiss C, et al. Thiol/disulfide exchange is a prerequisite for CXCR4-tropic HIV-1 envelope-mediated T-cell fusion during viral entry. Blood. 2004;103:1586-94 pubmed
Lahav J, Wijnen E, Hess O, Hamaia S, Griffiths D, Makris M, et al. Enzymatically catalyzed disulfide exchange is required for platelet adhesion to collagen via integrin alpha2beta1. Blood. 2003;102:2085-92 pubmed
Davis C, Raulston J, Wyrick P. Protein disulfide isomerase, a component of the estrogen receptor complex, is associated with Chlamydia trachomatis serovar E attached to human endometrial epithelial cells. Infect Immun. 2002;70:3413-8 pubmed
Nies A, Konig J, Cui Y, Brom M, Spring H, Keppler D. Structural requirements for the apical sorting of human multidrug resistance protein 2 (ABCC2). Eur J Biochem. 2002;269:1866-76 pubmed
Vranka J, Mokashi A, Keene D, Tufa S, Corson G, Sussman M, et al. Selective intracellular retention of extracellular matrix proteins and chaperones associated with pseudoachondroplasia. Matrix Biol. 2001;20:439-50 pubmed
Barkalow F, Barkalow K, Mayadas T. Dimerization of P-selectin in platelets and endothelial cells. Blood. 2000;96:3070-7 pubmed
Bennett T, Edwards B, Sklar L, Rogelj S. Sulfhydryl regulation of L-selectin shedding: phenylarsine oxide promotes activation-independent L-selectin shedding from leukocytes. J Immunol. 2000;164:4120-9 pubmed
Tanaka S, Uehara T, Nomura Y. Up-regulation of protein-disulfide isomerase in response to hypoxia/brain ischemia and its protective effect against apoptotic cell death. J Biol Chem. 2000;275:10388-93 pubmed
Mezghrani A, Courageot J, Mani J, Pugniere M, Bastiani P, Miquelis R. Protein-disulfide isomerase (PDI) in FRTL5 cells. pH-dependent thyroglobulin/PDI interactions determine a novel PDI function in the post-endoplasmic reticulum of thyrocytes. J Biol Chem. 2000;275:1920-9 pubmed
Ryser H, Levy E, Mandel R, DiSciullo G. Inhibition of human immunodeficiency virus infection by agents that interfere with thiol-disulfide interchange upon virus-receptor interaction. Proc Natl Acad Sci U S A. 1994;91:4559-63 pubmed
Kaetzel C, Rao C, Lamm M. Protein disulphide-isomerase from human placenta and rat liver. Purification and immunological characterization with monoclonal antibodies. Biochem J. 1987;241:39-47 pubmed
image
image 1 :
Invitrogen MA3-019 image 1
Immunofluorescent analysis of Phalloidin (blue) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 (Product # 37525) for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with Dylight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin (Product # 21830) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (red) were stained with DRAQ5 (Product # 62254) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
product information
Product Type :
Antibody
Product Name :
PDI Monoclonal Antibody (RL90)
Catalog # :
MA3-019
Quantity :
100 µL
Price :
US 425.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Hamster, Human, Mouse, Porcine, Rat
Applications :
Flow Cytometry: 0.5-1 µg/test, Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): 1:100, Immunohistochemistry (Paraffin): 1:100, Immunomicroscopy: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:200-1:2,000
Species :
Hamster, Human, Mouse, Porcine, Rat
Clone :
RL90
Isotype :
IgG2a
Storage :
-20° C, Avoid Freeze/Thaw Cycles
Description :
Protein disulfide isomerase (PDI) is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, the protein seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. It may therefore cause structural modifications of exofacial proteins. Inside the cell, PDI seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, it functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). PDI may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.
Immunogen :
Purified rat PDI protein.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5-1 µg/test, Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): 1:100, Immunohistochemistry (Paraffin): 1:100, Immunomicroscopy: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:200-1:2,000
Aliases :
1810041F13Rik; AI661267; CaO19.12595; cellular thyroid hormone binding protein; cellular thyroid hormone-binding protein; CLCRP1; collagen prolyl 4-hydroxylase beta; DSI; Endoplasmic reticulum resident protein 59; Eps1p; ER protein 59; ERBA2L; ERp59; GIT; glutathione-insulin transhydrogenase; I79_002399; LOW QUALITY PROTEIN: protein disulfide-isomerase A2; MFP1; P4HB; p4hb.L; P4Hbeta; p55; p58; Pancreas-specific protein disulfide isomerase; pancreatic protein disulfide isomerase; PDA2; PDI; PDI (E.C.5.3.4.1); PDI1; PDI1P; Pdia1; pdia2; pdia2.L; PDIP; Pdipl; PDIR; PHDB; PO4DB; PO4HB; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; PROHB; prolyl 4-hydroxylase beta polypeptide; Prolyl 4-hydroxylase subunit beta; prolyl 4-hydroxylase subunit beta L homeolog; prolyl 4-hydroxylase subunit beta L homeolog; prolyl 4-hydroxylase, beta polypeptide L homeolog; prolyl 4-hydroxylase, beta polypeptide; prolyl 4-hydroxylase, beta subunit; protein disulfide isomerase; protein disulfide isomerase (pancreas) like; Protein disulfide isomerase (Prolyl 4-hydroxylase, beta polypeptide); protein disulfide isomerase A2; protein disulfide isomerase associated 2; protein disulfide isomerase family A member 2; protein disulfide isomerase family A member 2 L homeolog; protein disulfide isomerase family A, member 1; protein disulfide isomerase family A, member 2; protein disulfide isomerase PDI1; protein disulfide isomerase, pancreatic; protein disulfide isomerase/oxidoreductase; protein disulfide isomerase-associated 1; protein disulfide isomerase-associated 2; protein disulfide-isomerase; Protein disulfide-isomerase A2; protocollagen hydroxylase; Rho GDP dissociation inhibitor gamma; testicular secretory protein Li 32; Thbp; Thioredoxin-related glycoprotein 1; thyroid hormone-binding protein p55; TRG1; XELAEV_18043753mg; XELAEV_18045582mg; XPDIp; YCL043C; YCL313; YCL43C
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA