PDI Monoclonal Antibody (RL77) | Invitrogen MA3-018 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PDI Monoclonal Antibody (RL77)

catalog :

MA3-018

quantity :

100 µL

price :

US 425.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

RL77

reactivity :

African green monkey, Chinese hamsters, human, mouse, rat, dogs, pigs , Xenopus laevis, rhesus macaque

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 40

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 2d	Braganza A, Li J, Zeng X, Yates N, Dey N, Andrews J, et al. UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase. J Biol Chem. 2017;292:2470-2484 pubmed publisher
immunocytochemistry; mouse; 1:100; loading ...; fig s3b	Matsunaga K, Taoka M, Isobe T, Izumi T. Rab2a and Rab27a cooperatively regulate the transition from granule maturation to exocytosis through the dual effector Noc2. J Cell Sci. 2017;130:541-550 pubmed publisher
western blot; human; loading ...; fig s4	Van Damme P, Kalvik T, Starheim K, Jonckheere V, Myklebust L, Menschaert G, et al. A Role for Human N-alpha Acetyltransferase 30 (Naa30) in Maintaining Mitochondrial Integrity. Mol Cell Proteomics. 2016;15:3361-3372 pubmed
immunocytochemistry; African green monkey; 1:200; fig 4	Stoica R, De Vos K, Paillusson S, Mueller S, Sancho R, Lau K, et al. ER-mitochondria associations are regulated by the VAPB-PTPIP51 interaction and are disrupted by ALS/FTD-associated TDP-43. Nat Commun. 2014;5:3996 pubmed publisher
immunoprecipitation; mouse; 35 ug/500 ug	Kenche H, Baty C, Vedagiri K, Shapiro S, Blumental Perry A. Cigarette smoking affects oxidative protein folding in endoplasmic reticulum by modifying protein disulfide isomerase. FASEB J. 2013;27:965-77 pubmed publisher
blocking or activating experiments; rhesus macaque	Calderon M, Guerrero C, Acosta O, Lopez S, Arias C. Inhibiting rotavirus infection by membrane-impermeant thiol/disulfide exchange blockers and antibodies against protein disulfide isomerase. Intervirology. 2012;55:451-64 pubmed publisher
western blot; human	De Vos K, MÃ³rotz G, Stoica R, Tudor E, Lau K, Ackerley S, et al. VAPB interacts with the mitochondrial protein PTPIP51 to regulate calcium homeostasis. Hum Mol Genet. 2012;21:1299-311 pubmed publisher
immunocytochemistry; human	Mahoney D, Lefebvre C, Allan K, Brun J, Sanaei C, Baird S, et al. Virus-tumor interactome screen reveals ER stress response can reprogram resistant cancers for oncolytic virus-triggered caspase-2 cell death. Cancer Cell. 2011;20:443-56 pubmed publisher
immunocytochemistry; rat	Hao Y, Hao C, Perkinton M, Chan W, Chan H, Miller C, et al. GULP1 is a novel APP-interacting protein that alters APP processing. Biochem J. 2011;436:631-9 pubmed publisher
immunoprecipitation; human; 10 ug/ml western blot; human; 1 ug/ml	Swiatkowska M, Padula G, Michalec L, Stasiak M, Skurzynski S, Cierniewski C. Ero1alpha is expressed on blood platelets in association with protein-disulfide isomerase and contributes to redox-controlled remodeling of alphaIIbbeta3. J Biol Chem. 2010;285:29874-83 pubmed publisher
immunocytochemistry; human; 1:100	Bernasconi R, Pertel T, Luban J, Molinari M. A dual task for the Xbp1-responsive OS-9 variants in the mammalian endoplasmic reticulum: inhibiting secretion of misfolded protein conformers and enhancing their disposal. J Biol Chem. 2008;283:16446-54 pubmed publisher
flow cytometry; human immunoprecipitation; human western blot; human	Swiatkowska M, Szymanski J, Padula G, Cierniewski C. Interaction and functional association of protein disulfide isomerase with alphaVbeta3 integrin on endothelial cells. FEBS J. 2008;275:1813-23 pubmed publisher
immunocytochemistry; human	Garver W, Jelinek D, Francis G, Murphy B. The Niemann-Pick C1 gene is downregulated by feedback inhibition of the SREBP pathway in human fibroblasts. J Lipid Res. 2008;49:1090-102 pubmed publisher
western blot; human	Labriola L, Ferreira G, Montor W, Demasi M, Pimenta D, Lojudice F, et al. Prolactin-induced changes in protein expression in human pancreatic islets. Mol Cell Endocrinol. 2007;264:16-27 pubmed
flow cytometry; human; 10 ug/ml immunocytochemistry; human; 1:100	Ou W, Silver J. Role of protein disulfide isomerase and other thiol-reactive proteins in HIV-1 envelope protein-mediated fusion. Virology. 2006;350:406-17 pubmed
immunocytochemistry; human; 1:200	O Donnell V, LaRocco M, Duque H, Baxt B. Analysis of foot-and-mouth disease virus internalization events in cultured cells. J Virol. 2005;79:8506-18 pubmed
western blot; rat	Stillemark Billton P, Beck C, Boren J, Olofsson S. Relation of the size and intracellular sorting of apoB to the formation of VLDL 1 and VLDL 2. J Lipid Res. 2005;46:104-14 pubmed
	Lopes da Silva M, McCormack J, Burden J, Harrison Lavoie K, Ferraro F, Cutler D. A GBF1-Dependent Mechanism for Environmentally Responsive Regulation of ER-Golgi Transport. Dev Cell. 2019;49:786-801.e6 pubmed publisher
	Talebi A, Dehairs J, Rambow F, Rogiers A, Nittner D, Derua R, et al. Sustained SREBP-1-dependent lipogenesis as a key mediator of resistance to BRAF-targeted therapy. Nat Commun. 2018;9:2500 pubmed publisher
	Sun J, Jiang J, Lu K, Chen Q, Tao D, Chen Z. Therapeutic potential of ADAM17 modulation in gastric cancer through regulation of the EGFR and TNF-? signalling pathways. Mol Cell Biochem. 2017;426:17-26 pubmed publisher
	Kenche H, Ye Z, Vedagiri K, Richards D, Gao X, Tew K, et al. Adverse Outcomes Associated with Cigarette Smoke Radicals Related to Damage to Protein-disulfide Isomerase. J Biol Chem. 2016;291:4763-78 pubmed publisher
	Gladue D, O Donnell V, Baker Branstetter R, Holinka L, Pacheco J, Fernandez Sainz I, et al. Foot-and-mouth disease virus nonstructural protein 2C interacts with Beclin1, modulating virus replication. J Virol. 2012;86:12080-90 pubmed publisher
	Wang Y, Yao Y, Tang X, Wang Z. Mouse RIC-3, an endoplasmic reticulum chaperone, promotes assembly of the alpha7 acetylcholine receptor through a cytoplasmic coiled-coil domain. J Neurosci. 2009;29:12625-35 pubmed publisher
	Petrucelli A, Rak M, Grainger L, Goodrum F. Characterization of a novel Golgi apparatus-localized latency determinant encoded by human cytomegalovirus. J Virol. 2009;83:5615-29 pubmed publisher
	Schultz Norton J, McDonald W, Yates J, Nardulli A. Protein disulfide isomerase serves as a molecular chaperone to maintain estrogen receptor alpha structure and function. Mol Endocrinol. 2006;20:1982-95 pubmed
	Minogue P, Liu X, Ebihara L, Beyer E, Berthoud V. An aberrant sequence in a connexin46 mutant underlies congenital cataracts. J Biol Chem. 2005;280:40788-95 pubmed
	Wierenga C, Ibata K, Turrigiano G. Postsynaptic expression of homeostatic plasticity at neocortical synapses. J Neurosci. 2005;25:2895-905 pubmed
	Tao Y, Johnson N, Segaloff D. Constitutive and agonist-dependent self-association of the cell surface human lutropin receptor. J Biol Chem. 2004;279:5904-14 pubmed
	Knoblach B, Keller B, Groenendyk J, Aldred S, Zheng J, Lemire B, et al. ERp19 and ERp46, new members of the thioredoxin family of endoplasmic reticulum proteins. Mol Cell Proteomics. 2003;2:1104-19 pubmed
	Shames I, Fraser A, Colby J, Orfali W, Snipes G. Phenotypic differences between peripheral myelin protein-22 (PMP22) and myelin protein zero (P0) mutations associated with Charcot-Marie-Tooth-related diseases. J Neuropathol Exp Neurol. 2003;62:751-64 pubmed
	Clark R, Li S, Pearson D, Leidal K, Clark J, Denning G, et al. Regulation of calreticulin expression during induction of differentiation in human myeloid cells. Evidence for remodeling of the endoplasmic reticulum. J Biol Chem. 2002;277:32369-78 pubmed
	Sargsyan E, Baryshev M, Szekely L, Sharipo A, Mkrtchian S. Identification of ERp29, an endoplasmic reticulum lumenal protein, as a new member of the thyroglobulin folding complex. J Biol Chem. 2002;277:17009-15 pubmed
	Li P, Nakanishi A, Clark S, Kasamatsu H. Formation of transitory intrachain and interchain disulfide bonds accompanies the folding and oligomerization of simian virus 40 Vp1 in the cytoplasm. Proc Natl Acad Sci U S A. 2002;99:1353-8 pubmed
	Torres V, Cai Y, Chen X, Wu G, Geng L, Cleghorn K, et al. Vascular expression of polycystin-2. J Am Soc Nephrol. 2001;12:1-9 pubmed
	Colby J, Nicholson R, Dickson K, Orfali W, Naef R, Suter U, et al. PMP22 carrying the trembler or trembler-J mutation is intracellularly retained in myelinating Schwann cells. Neurobiol Dis. 2000;7:561-73 pubmed
	Berryman M, Bretscher A. Identification of a novel member of the chloride intracellular channel gene family (CLIC5) that associates with the actin cytoskeleton of placental microvilli. Mol Biol Cell. 2000;11:1509-21 pubmed
	Bennett T, Edwards B, Sklar L, Rogelj S. Sulfhydryl regulation of L-selectin shedding: phenylarsine oxide promotes activation-independent L-selectin shedding from leukocytes. J Immunol. 2000;164:4120-9 pubmed
	Basu S, Srivastava P. Calreticulin, a peptide-binding chaperone of the endoplasmic reticulum, elicits tumor- and peptide-specific immunity. J Exp Med. 1999;189:797-802 pubmed
	Kuznetsov G, Chen L, Nigam S. Several endoplasmic reticulum stress proteins, including ERp72, interact with thyroglobulin during its maturation. J Biol Chem. 1994;269:22990-5 pubmed
	Kaetzel C, Rao C, Lamm M. Protein disulphide-isomerase from human placenta and rat liver. Purification and immunological characterization with monoclonal antibodies. Biochem J. 1987;241:39-47 pubmed

image

image 1 :

Immunofluorescent analysis of PDI using anti-PDI monoclonal antibody (Product# MA3-018) shows staining in HMVEC Cells.

product information

Product Type :

Antibody

Product Name :

PDI Monoclonal Antibody (RL77)

Catalog # :

MA3-018

Quantity :

100 µL

Price :

US 425.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Canine, Hamster, Human, Mouse, Porcine, Rat, Xenopus laevis

Applications :

Flow Cytometry: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry: 1:100, Immunoprecipitation: Assay-dependent, Western Blot: 1:200-1:2,000

Species :

Canine, Hamster, Human, Mouse, Porcine, Rat, Xenopus laevis

Clone :

RL77

Isotype :

IgG2b

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Protein disulfide isomerase (PDI) is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, the protein seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. It may therefore cause structural modifications of exofacial proteins. Inside the cell, PDI seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, it functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). PDI may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.

Immunogen :

Purified rat PDI protein.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry: 1:100, Immunoprecipitation: Assay-dependent, Western Blot: 1:200-1:2,000

Aliases :

1810041F13Rik; AI661267; CaO19.12595; cellular thyroid hormone binding protein; cellular thyroid hormone-binding protein; CLCRP1; collagen prolyl 4-hydroxylase beta; DSI; Endoplasmic reticulum resident protein 59; Eps1p; ER protein 59; ERBA2L; ERp59; GIT; glutathione-insulin transhydrogenase; I79_002399; LOW QUALITY PROTEIN: protein disulfide-isomerase A2; MFP1; P4HB; p4hb.L; P4Hbeta; p55; p58; Pancreas-specific protein disulfide isomerase; pancreatic protein disulfide isomerase; PDA2; PDI; PDI (E.C.5.3.4.1); PDI1; PDI1P; Pdia1; pdia2; pdia2.L; PDIP; Pdipl; PDIR; PHDB; PO4DB; PO4HB; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; PROHB; prolyl 4-hydroxylase beta polypeptide; Prolyl 4-hydroxylase subunit beta; prolyl 4-hydroxylase subunit beta L homeolog; prolyl 4-hydroxylase subunit beta L homeolog; prolyl 4-hydroxylase, beta polypeptide L homeolog; prolyl 4-hydroxylase, beta polypeptide; prolyl 4-hydroxylase, beta subunit; protein disulfide isomerase; protein disulfide isomerase (pancreas) like; Protein disulfide isomerase (Prolyl 4-hydroxylase, beta polypeptide); protein disulfide isomerase A2; protein disulfide isomerase associated 2; protein disulfide isomerase family A member 2; protein disulfide isomerase family A member 2 L homeolog; protein disulfide isomerase family A, member 1; protein disulfide isomerase family A, member 2; protein disulfide isomerase PDI1; protein disulfide isomerase, pancreatic; protein disulfide isomerase/oxidoreductase; protein disulfide isomerase-associated 1; protein disulfide isomerase-associated 2; protein disulfide-isomerase; Protein disulfide-isomerase A2; protocollagen hydroxylase; Rho GDP dissociation inhibitor gamma; testicular secretory protein Li 32; Thbp; Thioredoxin-related glycoprotein 1; thyroid hormone-binding protein p55; TRG1; XELAEV_18043753mg; XELAEV_18045582mg; XPDIp; YCL043C; YCL313; YCL43C

more info or order :

Invitrogen product webpage