GRP94 Monoclonal Antibody (9G10) | Invitrogen MA3-016 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

GRP94 Monoclonal Antibody (9G10)

catalog :

MA3-016

quantity :

100 µL

price :

US 425.00

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

9G10

reactivity :

Chinese hamsters, human, mouse, rat, chicken, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human	Zhao Y, Zhang H, Lam C, Hao Q, Lee H. Cytosolic CD38 protein forms intact disulfides and is active in elevating intracellular cyclic ADP-ribose. J Biol Chem. 2011;286:22170-7 pubmed publisher
western blot; mouse	Panaretakis T, Kepp O, Brockmeier U, Tesniere A, Bjorklund A, Chapman D, et al. Mechanisms of pre-apoptotic calreticulin exposure in immunogenic cell death. EMBO J. 2009;28:578-90 pubmed publisher
western blot; mouse	Gramolini A, Kislinger T, Alikhani Koopaei R, Fong V, Thompson N, Isserlin R, et al. Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses. Mol Cell Proteomics. 2008;7:519-33 pubmed
immunohistochemistry; mouse western blot; mouse	Wanderling S, Simen B, Ostrovsky O, Ahmed N, Vogen S, Gidalevitz T, et al. GRP94 is essential for mesoderm induction and muscle development because it regulates insulin-like growth factor secretion. Mol Biol Cell. 2007;18:3764-75 pubmed
western blot; mouse	Recktenwald C, Mendler S, Lichtenfels R, Kellner R, Seliger B. Influence of Ki-ras-driven oncogenic transformation on the protein network of murine fibroblasts. Proteomics. 2007;7:385-98 pubmed
western blot; rat	Stillemark Billton P, Beck C, Boren J, Olofsson S. Relation of the size and intracellular sorting of apoB to the formation of VLDL 1 and VLDL 2. J Lipid Res. 2005;46:104-14 pubmed
	Asquith K, Baleato R, McLaughlin E, Nixon B, Aitken R. Tyrosine phosphorylation activates surface chaperones facilitating sperm-zona recognition. J Cell Sci. 2004;117:3645-57 pubmed
	Gidalevitz T, Biswas C, Ding H, Schneidman Duhovny D, Wolfson H, Stevens F, et al. Identification of the N-terminal peptide binding site of glucose-regulated protein 94. J Biol Chem. 2004;279:16543-52 pubmed
	Robert J, Gantress J, Cohen N, Maniero G. Xenopus as an experimental model for studying evolution of hsp--immune system interactions. Methods. 2004;32:42-53 pubmed
	Ishiwatari Hayasaka H, Maruya M, Sreedhar A, Nemoto T, Csermely P, Yahara I. Interaction of neuropeptide Y and Hsp90 through a novel peptide binding region. Biochemistry. 2003;42:12972-80 pubmed
	Calvert M, Digilio L, Herr J, Coonrod S. Oolemmal proteomics--identification of highly abundant heat shock proteins and molecular chaperones in the mature mouse egg and their localization on the plasma membrane. Reprod Biol Endocrinol. 2003;1:27 pubmed
	Khanna R, Myers M, Laine M, Papazian D. Glycosylation increases potassium channel stability and surface expression in mammalian cells. J Biol Chem. 2001;276:34028-34 pubmed
	Mengesdorf T, Althausen S, Oberndorfer I, Paschen W. Response of neurons to an irreversible inhibition of endoplasmic reticulum Ca(2+)-ATPase: relationship between global protein synthesis and expression and translation of individual genes. Biochem J. 2001;356:805-12 pubmed
	Bogan J, Lodish H. Two compartments for insulin-stimulated exocytosis in 3T3-L1 adipocytes defined by endogenous ACRP30 and GLUT4. J Cell Biol. 1999;146:609-20 pubmed
	Gaudin Y. Folding of rabies virus glycoprotein: epitope acquisition and interaction with endoplasmic reticulum chaperones. J Virol. 1997;71:3742-50 pubmed
	Hughes E, Hammond C, Cresswell P. Misfolded major histocompatibility complex class I heavy chains are translocated into the cytoplasm and degraded by the proteasome. Proc Natl Acad Sci U S A. 1997;94:1896-901 pubmed
	Nauseef W, McCormick S, Clark R. Calreticulin functions as a molecular chaperone in the biosynthesis of myeloperoxidase. J Biol Chem. 1995;270:4741-7 pubmed
	Edwards D, Weigel N, Schrader W, O Malley B, McGuire W. Structural analysis of chicken oviduct progesterone receptor using monoclonal antibodies to the subunit B protein. Biochemistry. 1984;23:4427-35 pubmed
	Sargan D, Tsai M, O Malley B. hsp108,,,,,, a novel heat shock inducible protein of chicken. Biochemistry. 1986;25:6252-8 pubmed

image

image 1 :

Immunofluorescent analysis of Glucose-Regulated Protein 94 using Glucose-Regulated Protein 94 Monoclonal Antibody (9G10) (Product# MA3-016 ) shows staining in C6 Cells. Glucose-Regulated Protein 94 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucose-Regulated Protein 94 (Product# MA3-016 ) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

image 2 :

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse breast tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Glucose Regulated Protein 94 (MA3-016) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

product information

Product Type :

Antibody

Product Name :

GRP94 Monoclonal Antibody (9G10)

Catalog # :

MA3-016

Quantity :

100 µL

Price :

US 425.00

Clonality :

Monoclonal

Host :

Rat

Reactivity :

Bovine, Chicken, Hamster, Human, Mouse, Rat

Applications :

Immunocytochemistry: 1:250, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: Assay-dependent, Western Blot: 1:100-1:1,000

Species :

Bovine, Chicken, Hamster, Human, Mouse, Rat

Clone :

9G10

Isotype :

IgG2a

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

GRP94 is a 803 amino acid protein belonging to the heat shock protein 90 family. It acts as a molecular chaperone that functions in the processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulates CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC). It plays a central role in innate as well as acquired immunity, maturation and chemotaxis of dendritic cells, Ab production, cross-priming, as a potential marker in breast cancer and is a peptide acceptor in endoplasmic reticulum and an accessory to peptide loading of MHC class I molecules. Expression of GRP94 suppressed A23187-induced apoptosis and stabilized calcium homeostasis. GRP94 is expressed in melanoma or liver metastases of colon carcinoma cells, human gastric carcinoma BGC-823 cells.

Immunogen :

Chick oviduct GRP94.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:250, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: Assay-dependent, Western Blot: 1:100-1:1,000

Aliases :

108K heat shock protein; 108K HSP; 94 kDa glucose-regulated protein; 94 kDa glucose-regulated protein {ECO:0000250; 98 kDa protein kinase; ecgp; endoplasmic reticulum resident protein 99; Endoplasmin; endoplasmin {ECO:0000250; endoplasmin-like protein; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; ERp99; glucose-regulated protein; glucose-regulated protein GRP94; glucose-regulated protein GRP94 precursor; GP96; gp96 homolog; gp96/GRP94; GRP94; GRP-94; heat shock 108 kDa protein; heat shock protein 108; heat shock protein 108.; heat shock protein 90 beta family member 1; heat shock protein 90 kDa beta member 1; heat shock protein 90 kDa beta member 1 {ECO:0000250; heat shock protein 90, beta (Grp94), member 1; heat shock protein 90, beta, member 1; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; heat shock protein 90kDa beta family member 1 L homeolog; heat shock protein 90kDa beta, member 1; heat shock protein gp96; HEL35; HEL-S-125m; HSP 108; hsp108; HSP90 beta; hsp90b1; hsp90b1 {ECO:0000250; hsp90b1.L; Polymorphic tumor rejection antigen 1; PPK 98; Ppk 98; a protein kinase; ppk98; stress-inducible tumor rejection antigen gp96; TA-3; Targ2; tra1; Tra-1; transferrin-binding protein; transforming growth factor alpha regulated gene 2; tumor rejection antigen; tumor rejection antigen (gp96) 1; tumor rejection antigen 1; Tumor rejection antigen gp96; UniProtKB:P08113}; XELAEV_18017384mg

more info or order :

Invitrogen product webpage