HSP27 Monoclonal Antibody (G3.1) | Invitrogen MA3-015 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HSP27 Monoclonal Antibody (G3.1)

catalog :

MA3-015

quantity :

100 µL

price :

US 425.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

G3.1

reactivity :

human, mouse, rat, dogs

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 32

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000	Frankenberg N, Lamb G, Overgaard K, Murphy R, Vissing K. Small heat shock proteins translocate to the cytoskeleton in human skeletal muscle following eccentric exercise independently of phosphorylation. J Appl Physiol (1985). 2014;116:1463-72 pubmed publisher
western blot; human; 1:500	Calamini B, Silva M, Madoux F, Hutt D, Khanna S, Chalfant M, et al. Small-molecule proteostasis regulators for protein conformational diseases. Nat Chem Biol. 2011;8:185-96 pubmed publisher
immunohistochemistry; human; 1:400	Rantanen T, Honkanen T, Paavonen T, Rantanen L, Oksala N. Altered expression of HSP27 and HSP70 in distal oesophageal mucosa in patients with gastro-oesophageal reflux disease subjected to fundoplication. Eur J Surg Oncol. 2011;37:168-74 pubmed publisher
immunohistochemistry; human	Ozger H, Eralp L, Atalar A, Toker B, Esberk Ateş L, Sungur M, et al. [The effect of resistance-related proteins on the prognosis and survival of patients with osteosarcoma: an immunohistochemical analysis]. Acta Orthop Traumatol Turc. 2009;43:28-34 pubmed publisher
immunohistochemistry; mouse; 1:500	Niwa M, Hara A, Taguchi A, Aoki H, Kozawa O, Mori H. Spatiotemporal expression of Hsp20 and its phosphorylation in hippocampal CA1 pyramidal neurons after transient forebrain ischemia. Neurol Res. 2009;31:721-7 pubmed publisher
immunohistochemistry; mouse; 1:500	Marty B, Maire V, Gravier E, Rigaill G, Vincent Salomon A, Kappler M, et al. Frequent PTEN genomic alterations and activated phosphatidylinositol 3-kinase pathway in basal-like breast cancer cells. Breast Cancer Res. 2008;10:R101 pubmed publisher
western blot; human	Carlson R, Vavricka S, Eloranta J, Musch M, Arvans D, Kles K, et al. fMLP induces Hsp27 expression, attenuates NF-kappaB activation, and confers intestinal epithelial cell protection. Am J Physiol Gastrointest Liver Physiol. 2007;292:G1070-8 pubmed
western blot; human	Liu Y, Liu H, Han B, Zhang J. Identification of 14-3-3sigma as a contributor to drug resistance in human breast cancer cells using functional proteomic analysis. Cancer Res. 2006;66:3248-55 pubmed
western blot; human	Sommer S, Cui Y, Brewer G, Fuqua S. The c-Yes 3'-UTR contains adenine/uridine-rich elements that bind AUF1 and HuR involved in mRNA decay in breast cancer cells. J Steroid Biochem Mol Biol. 2005;97:219-29 pubmed
immunohistochemistry; human; 1:50	Joo M, Chi J, Lee H. Expressions of HSP70 and HSP27 in hepatocellular carcinoma. J Korean Med Sci. 2005;20:829-34 pubmed
western blot; human	Caraglia M, Marra M, Mancinelli F, d Ambrosio G, Massa R, Giordano A, et al. Electromagnetic fields at mobile phone frequency induce apoptosis and inactivation of the multi-chaperone complex in human epidermoid cancer cells. J Cell Physiol. 2005;204:539-48 pubmed
	Hao Q, Wang P, Dutta P, Chung S, Li Q, Wang K, et al. Comp34 displays potent preclinical antitumor efficacy in triple-negative breast cancer via inhibition of NUDT3-AS4, a novel oncogenic long noncoding RNA. Cell Death Dis. 2020;11:1052 pubmed publisher
	Christiansen D, Murphy R, Bangsbo J, Stathis C, Bishop D. Increased FXYD1 and PGC-1α mRNA after blood flow-restricted running is related to fibre type-specific AMPK signalling and oxidative stress in human muscle. Acta Physiol (Oxf). 2018;223:e13045 pubmed publisher
	Nguyen Ho Bouldoires T, ClapÃ©ron A, Mergey M, Wendum D, Desbois Mouthon C, Tahraoui S, et al. Mitogen-activated protein kinase-activated protein kinase 2 mediates resistance to hydrogen peroxide-induced oxidative stress in human hepatobiliary cancer cells. Free Radic Biol Med. 2015;89:34-46 pubmed publisher
	MorlÃ© A, Garrido C, Micheau O. Hyperthermia restores apoptosis induced by death receptors through aggregation-induced c-FLIP cytosolic depletion. Cell Death Dis. 2015;6:e1633 pubmed publisher
	Wen F, Li Y, Tsai H, Lin C, Li C, Liu C, et al. Down-regulation of heat shock protein 27 in neuronal cells and non-neuronal cells expressing mutant ataxin-3. FEBS Lett. 2003;546:307-14 pubmed
	Elpek G, Karaveli S, Simsek T, Keles N, Aksoy N. Expression of heat-shock proteins hsp27, hsp70 and hsp90 in malignant epithelial tumour of the ovaries. APMIS. 2003;111:523-30 pubmed
	Pignatelli D, Ferreira J, Soares P, Costa M, Magalhães M. Immunohistochemical study of heat shock proteins 27, 60 and 70 in the normal human adrenal and in adrenal tumors with suppressed ACTH production. Microsc Res Tech. 2003;61:315-23 pubmed
	Benvenuti S, Cramer R, Quinn C, Bruce J, Zvelebil M, Corless S, et al. Differential proteome analysis of replicative senescence in rat embryo fibroblasts. Mol Cell Proteomics. 2002;1:280-92 pubmed
	Wyttenbach A, Sauvageot O, Carmichael J, Diaz Latoud C, Arrigo A, Rubinsztein D. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin. Hum Mol Genet. 2002;11:1137-51 pubmed
	Wyttenbach A, Swartz J, Kita H, Thykjaer T, Carmichael J, Bradley J, et al. Polyglutamine expansions cause decreased CRE-mediated transcription and early gene expression changes prior to cell death in an inducible cell model of Huntington's disease. Hum Mol Genet. 2001;10:1829-45 pubmed
	Huang P, Liu J, Di A, Robinson N, Musch M, Kaetzel M, et al. Regulation of human CLC-3 channels by multifunctional Ca2+/calmodulin-dependent protein kinase. J Biol Chem. 2001;276:20093-100 pubmed
	Gerner C, Frohwein U, Gotzmann J, Bayer E, Gelbmann D, Bursch W, et al. The Fas-induced apoptosis analyzed by high throughput proteome analysis. J Biol Chem. 2000;275:39018-26 pubmed
	Soldes O, Kuick R, Thompson I, Hughes S, Orringer M, Iannettoni M, et al. Differential expression of Hsp27 in normal oesophagus, Barrett's metaplasia and oesophageal adenocarcinomas. Br J Cancer. 1999;79:595-603 pubmed
	Ciocca D, Green S, Elledge R, Clark G, Pugh R, Ravdin P, et al. Heat shock proteins hsp27 and hsp70: lack of correlation with response to tamoxifen and clinical course of disease in estrogen receptor-positive metastatic breast cancer (a Southwest Oncology Group Study). Clin Cancer Res. 1998;4:1263-6 pubmed
	Ito T, Kawabe R, Kurasono Y, Hara M, Kitamura H, Fujita K, et al. Expression of heat shock proteins in squamous cell carcinoma of the tongue: an immunohistochemical study. J Oral Pathol Med. 1998;27:18-22 pubmed
	Adams D, Hajj H, Edwards D, Bjercke R, McGuire W. Detection of a Mr 24,000 estrogen-regulated protein in human breast cancer by monoclonal antibodies. Cancer Res. 1983;43:4297-301 pubmed
	Ciocca D, Asch R, Adams D, McGuire W. Evidence for modulation of a 24K protein in human endometrium during the menstrual cycle. J Clin Endocrinol Metab. 1983;57:496-9 pubmed
	Adams D, McGuire W. Quantitative enzyme-linked immunosorbent assay for the estrogen-regulated Mr 24,000 protein in human breast tumors: correlation with estrogen and progesterone receptors. Cancer Res. 1985;45:2445-9 pubmed
	Dressler L, Ramzy I, Sledge G, McGuire W. A new marker of maturation in the cervix: the estrogen-regulated 24K protein. Obstet Gynecol. 1986;68:825-31 pubmed
	Moretti Rojas I, Fuqua S, Montgomery R, McGuire W. A cDNA for the estradiol-regulated 24K protein: control of mRNA levels in MCF-7 cells. Breast Cancer Res Treat. 1988;11:155-63 pubmed
	McGuire S, Fuqua S, Naylor S, Helin Davis D, McGuire W. Chromosomal assignments of human 27-kDa heat shock protein gene family. Somat Cell Mol Genet. 1989;15:167-71 pubmed

image

image 1 :

Western blot analysis of Heat Shock Protein 27 (HSP27) was performed by loading 50µg of the indicated whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP27 monoclonal antibody (Product # MA3-015) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

product information

Product Type :

Antibody

Product Name :

HSP27 Monoclonal Antibody (G3.1)

Catalog # :

MA3-015

Quantity :

100 µL

Price :

US 425.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Canine, Human, Non-human primate, Rat

Applications :

ELISA: Assay-dependent, Immunocytochemistry: 1:500, Immunohistochemistry (Paraffin): 1:500, Immunomicroscopy: Assay-dependent, Immunoprecipitation: 2 µL, Western Blot: 1:500-1:1,000

Species :

Canine, Human, Non-human primate, Rat

Clone :

G3.1

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.

Immunogen :

Partially purified human HSP27.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunocytochemistry: 1:500, Immunohistochemistry (Paraffin): 1:500, Immunomicroscopy: Assay-dependent, Immunoprecipitation: 2 µL, Western Blot: 1:500-1:1,000

Aliases :

27kDa; 28 kDa heat shock protein; CMT2F; DKFZp586P1322; epididymis secretory protein Li 102; estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; heat shock 25 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; heat shock protein 1; heat shock protein 27 kDa beta-1; heat shock protein B1; heat shock protein beta-1; heat shock protein family B (small) member 1; heat shock protein, 25 kDa; heat-shock protein; HEL-S-102; HMN2B; HS; HS.76067; HSP; HSP 25; HSP 27; Hsp25; HSP27; HSP28; HSPB1; p25; Phospho-HSP 27; SRP27; Stress-responsive protein 27; truncated hsp25

more info or order :

Invitrogen product webpage