HSC70 Monoclonal Antibody (13D3) | Invitrogen MA3-014 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HSC70 Monoclonal Antibody (13D3)

catalog :

MA3-014

quantity :

50 µL

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13D3

reactivity :

hamsters, African green monkey, human, mouse, rat, cat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 24

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:5000; loading ...; fig 2b	Ormeño F, Hormazabal J, Moreno J, Riquelme F, Rios J, Criollo A, et al. Chaperone Mediated Autophagy Degrades TDP-43 Protein and Is Affected by TDP-43 Aggregation. Front Mol Neurosci. 2020;13:19 pubmed publisher
western blot; mouse; loading ...; tbl 1	Cao M, Wu Y, Ashrafi G, McCartney A, Wheeler H, Bushong E, et al. Parkinson Sac Domain Mutation in Synaptojanin 1 Impairs Clathrin Uncoating at Synapses and Triggers Dystrophic Changes in Dopaminergic Axons. Neuron. 2017;93:882-896.e5 pubmed publisher
western blot; human; 1:1000; fig 6	NÃ¼ÃŸe J, Mirastschijski U, Waespy M, Oetjen J, Brandes N, Rebello O, et al. Two new isoforms of the human hepatoma-derived growth factor interact with components of the cytoskeleton. Biol Chem. 2016;397:417-36 pubmed publisher
western blot; human; 1:1000; fig 5	Yao Y, Wei W, Sun J, Chen L, Deng X, Ma L, et al. Proteomic analysis of exosomes derived from human lymphoma cells. Eur J Med Res. 2015;20:8 pubmed publisher
western blot; rat; 1:500	Reddy V, Kumar C, Raghu G, Reddy G. Expression and induction of small heat shock proteins in rat heart under chronic hyperglycemic conditions. Arch Biochem Biophys. 2014;558:1-9 pubmed publisher
immunohistochemistry; rat; 1:200 western blot; rat; 1:500	Reddy V, Raghu G, Reddy S, Pasupulati A, Suryanarayana P, Reddy G. Response of small heat shock proteins in diabetic rat retina. Invest Ophthalmol Vis Sci. 2013;54:7674-82 pubmed publisher
flow cytometry; African green monkey	Isa P, Sanchez Aleman M, Lopez S, Arias C. Dissecting the role of integrin subunits alpha 2 and beta 3 in rotavirus cell entry by RNA silencing. Virus Res. 2009;145:251-9 pubmed publisher
immunohistochemistry; human; 1:100 western blot; human; 1:500	David D, Ittner L, Gehrig P, Nergenau D, Shepherd C, Halliday G, et al. Beta-amyloid treatment of two complementary P301L tau-expressing Alzheimer's disease models reveals similar deregulated cellular processes. Proteomics. 2006;6:6566-77 pubmed
blocking or activating experiments; hamsters; 1:100 flow cytometry; hamsters; 1:25 blocking or activating experiments; African green monkey; 1:100 flow cytometry; African green monkey; 1:25	López T, Lopez S, Arias C. Heat shock enhances the susceptibility of BHK cells to rotavirus infection through the facilitation of entry and post-entry virus replication steps. Virus Res. 2006;121:74-83 pubmed
	Taguwa S, Yeh M, Rainbolt T, Nayak A, Shao H, Gestwicki J, et al. Zika Virus Dependence on Host Hsp70 Provides a Protective Strategy against Infection and Disease. Cell Rep. 2019;26:906-920.e3 pubmed publisher
	Ghosh A, Desai A, Ravi V, Narayanappa G, Tyagi B. Chikungunya Virus Interacts with Heat Shock Cognate 70 Protein to Facilitate Its Entry into Mosquito Cell Line. Intervirology. 2017;60:247-262 pubmed publisher
	Reeg S, Jung T, Castro J, Davies K, Henze A, Grune T. The molecular chaperone Hsp70 promotes the proteolytic removal of oxidatively damaged proteins by the proteasome. Free Radic Biol Med. 2016;99:153-166 pubmed publisher
	Hegmans J, Bard M, Hemmes A, Luider T, Kleijmeer M, Prins J, et al. Proteomic analysis of exosomes secreted by human mesothelioma cells. Am J Pathol. 2004;164:1807-15 pubmed
	Guerrero C, Bouyssounade D, Zárate S, Isa P, López T, Espinosa R, et al. Heat shock cognate protein 70 is involved in rotavirus cell entry. J Virol. 2002;76:4096-102 pubmed
	Thompson H, Clarkson P, Scordilis S. The repeated bout effect and heat shock proteins: intramuscular HSP27 and HSP70 expression following two bouts of eccentric exercise in humans. Acta Physiol Scand. 2002;174:47-56 pubmed
	Fourie A, Peterson P, Yang Y. Characterization and regulation of the major histocompatibility complex-encoded proteins Hsp70-Hom and Hsp70-1/2. Cell Stress Chaperones. 2001;6:282-95 pubmed
	Bernstein S, Liu A, Hansen B, Somiari R. Heat shock cognate-70 gene expression declines during normal aging of the primate retina. Invest Ophthalmol Vis Sci. 2000;41:2857-62 pubmed
	Polanowska Grabowska R, Simon C, Falchetto R, Shabanowitz J, Hunt D, Gear A. Platelet adhesion to collagen under flow causes dissociation of a phosphoprotein complex of heat-shock proteins and protein phosphatase 1. Blood. 1997;90:1516-26 pubmed
	Nakano M, Mann D, Knowlton A. Blocking the endogenous increase in HSP 72 increases susceptibility to hypoxia and reoxygenation in isolated adult feline cardiocytes. Circulation. 1997;95:1523-31 pubmed
	Leung S, Hightower L. A 16-kDa protein functions as a new regulatory protein for Hsc70 molecular chaperone and is identified as a member of the Nm23/nucleoside diphosphate kinase family. J Biol Chem. 1997;272:2607-14 pubmed
	Nunes S, Calderwood S. Heat shock factor-1 and the heat shock cognate 70 protein associate in high molecular weight complexes in the cytoplasm of NIH-3T3 cells. Biochem Biophys Res Commun. 1995;213:1-6 pubmed
	Allen R, O Brien D, Eddy E. A novel hsp70-like protein (P70) is present in mouse spermatogenic cells. Mol Cell Biol. 1988;8:828-32 pubmed
	Maekawa M, O Brien D, Allen R, Eddy E. Heat-shock cognate protein (hsc71) and related proteins in mouse spermatogenic cells. Biol Reprod. 1989;40:843-52 pubmed
	Terlecky S, Chiang H, Olson T, Dice J. Protein and peptide binding and stimulation of in vitro lysosomal proteolysis by the 73-kDa heat shock cognate protein. J Biol Chem. 1992;267:9202-9 pubmed

image

image 1 :

Western blot analysis of Heat Shock Protein 70 (HSP70) was performed by loading 50µg of the indicated whole cell lysates and 15µl of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP70 monoclonal antibody (Product # MA3-014) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgM-HRP secondary antibody (Product # 31440) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

image 2 :

Immunofluorescent analysis of Heat Shock Protein 70 using Heat Shock Protein 70 Monoclonal antibody (13D3) (Product# MA3-014) shows staining in NIH-3T3 cells. Heat Shock Protein 70 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 70 (Product# MA3-014) at a dilution of 1:20-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

HSC70 Monoclonal Antibody (13D3)

Catalog # :

MA3-014

Quantity :

50 µL

Price :

US 399.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Feline, Human, Mouse, Non-human primate, Rat

Applications :

Immunocytochemistry: 1:250, Immunohistochemistry (Frozen): 1:20-1:200, Immunoprecipitation: 2 µL, Western Blot: 1:1,000-1:4,000

Species :

Feline, Human, Mouse, Non-human primate, Rat

Clone :

13D3

Isotype :

IgM

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. HSC70 (also known as HSC71, HSC73, HSP73, p72, prp73) is expressed constitutively and is slightly heat-inducible. HSC70 binds to the exposed loop of clathrin light chains to promote uncoating and can also bind the cytoskeleton which may facilitate cytoskeletal rearrangements. HSC70 has been shown to stimulate lysosomal degradation of intracellular proteins and to retard both aggregation and folding of mitochondrial precursor proteins in vitro.

Immunogen :

Mouse spermatogenic cell protein.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:250, Immunohistochemistry (Frozen): 1:20-1:200, Immunoprecipitation: 2 µL, Western Blot: 1:1,000-1:4,000

Aliases :

2410008N15Rik; AAG2; constitutive heat shock protein 70; constitutively expressed heat shock protein; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 72p; FAM10A1; Heat shock 70 kDa protein 8; heat shock 70kd protein 10; heat shock 70kd protein 10 (HSC71); heat shock 70kD protein 8; heat shock 70kDa protein 8; heat shock cognate; heat shock cognate 70; heat shock cognate 71 kD protein; heat shock cognate 71 kDa protein; heat shock cognate 71 kDa protein-like protein; heat shock cognate 71-kd protein; heat shock cognate hsc73; heat shock cognate protein 54; Heat shock cognate protein 70; heat shock protein 70 cognate; heat shock protein 8; heat shock protein A8; heat shock protein cognate 70; heat shock protein family A (Hsp70) member 8; HEL-33; HEL-S-72p; HIP; HSC54; Hsc70; Hsc70 ATPase; HSC70 protein; Hsc71; Hsc73; HSP70; HSP71; HSP73; Hspa10; Hspa8; hspa8 protein; I79_022974; LAP; LAP1; LAP-1; lipopolysaccharide-associated protein 1; LPS-associated protein 1; MGC131511; MGC29929; NIP71; N-myristoyltransferase inhibitor protein 71; Protein FAM10A1; similar to 7O KDa heat shock cognate protein; SNC6; unnamed protein product; wu:fb01g06; wu:fi48b06

more info or order :

Invitrogen product webpage