HSP60 Monoclonal Antibody (4B9/89) | Invitrogen MA3-012 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HSP60 Monoclonal Antibody (4B9/89)

catalog :

MA3-012

quantity :

100 µg

price :

US 425.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4B9/89

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; fig s3	Zhang T, Du W, Wilson A, Namekawa S, Andreassen P, Meetei A, et al. Fancd2 in vivo interaction network reveals a non-canonical role in mitochondrial function. Sci Rep. 2017;7:45626 pubmed publisher
western blot; human; fig 4	Patra M, Mahata S, Padhan D, Sen M. CCN6 regulates mitochondrial function. J Cell Sci. 2016;129:2841-51 pubmed publisher
western blot; human	Ahuja N, Schwer B, Carobbio S, Waltregny D, North B, Castronovo V, et al. Regulation of insulin secretion by SIRT4, a mitochondrial ADP-ribosyltransferase. J Biol Chem. 2007;282:33583-92 pubmed
blocking or activating experiments; mouse; 15 ug/ml	Osterloh A, Kalinke U, Weiss S, Fleischer B, Breloer M. Synergistic and differential modulation of immune responses by Hsp60 and lipopolysaccharide. J Biol Chem. 2007;282:4669-80 pubmed
	Higashi H, Maejima T, Lee L, Yamazaki Y, Hottiger M, Singh S, et al. A Study into the ADP-Ribosylome of IFN-γ-Stimulated THP-1 Human Macrophage-like Cells Identifies ARTD8/PARP14 and ARTD9/PARP9 ADP-Ribosylation. J Proteome Res. 2019;18:1607-1622 pubmed publisher
	Solis G, Kardakaris R, Valentine E, Bar Peled L, Chen A, Blewett M, et al. Translation attenuation by minocycline enhances longevity and proteostasis in old post-stress-responsive organisms. elife. 2018;7: pubmed publisher
	Schueder F, Lara Gutiérrez J, Beliveau B, Saka S, Sasaki H, Woehrstein J, et al. Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT. Nat Commun. 2017;8:2090 pubmed publisher
	Märker T, Kriebel J, Wohlrab U, Burkart V, Habich C. Adipocytes from New Zealand obese mice exhibit aberrant proinflammatory reactivity to the stress signal heat shock protein 60. J Diabetes Res. 2014;2014:187153 pubmed publisher
	Schwer B, North B, Frye R, Ott M, Verdin E. The human silent information regulator (Sir)2 homologue hSIRT3 is a mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase. J Cell Biol. 2002;158:647-57 pubmed
	Wu R, Zhao Y, Plopper C, Chang M, Chmiel K, Cross J, et al. Differential expression of stress proteins in nonhuman primate lung and conducting airway after ozone exposure. Am J Physiol. 1999;277:L511-22 pubmed publisher
	Bassan M, Zamostiano R, Giladi E, Davidson A, Wollman Y, Pitman J, et al. The identification of secreted heat shock 60 -like protein from rat glial cells and a human neuroblastoma cell line. Neurosci Lett. 1998;250:37-40 pubmed
	Lau S, Patnaik N, Sayen M, Mestril R. Simultaneous overexpression of two stress proteins in rat cardiomyocytes and myogenic cells confers protection against ischemia-induced injury. Circulation. 1997;96:2287-94 pubmed
	Morris S, Cumming D, Latchman D, Yellon D. Specific induction of the 70-kD heat stress proteins by the tyrosine kinase inhibitor herbimycin-A protects rat neonatal cardiomyocytes. A new pharmacological route to stress protein expression?. J Clin Invest. 1996;97:706-12 pubmed
	Soltys B, Gupta R. Immunoelectron microscopic localization of the 60-kDa heat shock chaperonin protein (Hsp60) in mammalian cells. Exp Cell Res. 1996;222:16-27 pubmed
	Jindal S, Dudani A, Singh B, Harley C, Gupta R. Primary structure of a human mitochondrial protein homologous to the bacterial and plant chaperonins and to the 65-kilodalton mycobacterial antigen. Mol Cell Biol. 1989;9:2279-83 pubmed
	Langer T, Pfeifer G, Martin J, Baumeister W, Hartl F. Chaperonin-mediated protein folding: GroES binds to one end of the GroEL cylinder, which accommodates the protein substrate within its central cavity. EMBO J. 1992;11:4757-65 pubmed

image

image 1 :

Immunofluorescent analysis of Heat Shock Protein 60 using Anti-Heat Shock Protein 60 Monoclonal Antibody (4B9/89) (Product# MA3-012) shows staining in Hela Cells. Heat Shock Protein 60 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 60 (Product# MA3-012) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

HSP60 Monoclonal Antibody (4B9/89)

Catalog # :

MA3-012

Quantity :

100 µg

Price :

US 425.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Non-human primate, Rat

Applications :

ELISA: Assay-dependent, Flow Cytometry: 1-20 µg/mL, Immunocytochemistry: 1:50-1:60, Immunohistochemistry: 1:200, Immunoprecipitation: 2 µg, Western Blot: 1:100-1:1,000

Species :

Human, Mouse, Non-human primate, Rat

Clone :

4B9/89

Isotype :

IgG2a

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.

Immunogen :

Human placental HSP60.

Format :

Lyophilized

Applications w/Dilutions :

ELISA: Assay-dependent, Flow Cytometry: 1-20 µg/mL, Immunocytochemistry: 1:50-1:60, Immunohistochemistry: 1:200, Immunoprecipitation: 2 µg, Western Blot: 1:100-1:1,000

Aliases :

60 kDa chaperonin; 60 kDa heat shock protein; 60 kDa heat shock protein, mitochondrial; 60kDa; Chaperonin 60; CPN60; GROEL; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1; heat shock 60kDa protein 1 (chaperonin); Heat shock protein; heat shock protein 1 (chaperonin); heat shock protein 60; heat shock protein 60 (liver); heat shock protein 65; heat shock protein family D (Hsp60) member 1; heat shock protein, 60 kDa; HLD4; HSP; Hsp60; HSP-60; Hsp60s1; HSP65; HSP-65; Hspd1; Hspd1-30p; HuCHA60; hypothetical protein; I79_011398; mitochondrial matrix protein P1; P1 protein; P60 lymphocyte protein; RCJMB04_7g5; short heat shock protein 60 Hsp60s1; SPG13; Unknown (protein for IMAGE:7943409)

more info or order :

Invitrogen product webpage