HSP70 Monoclonal Antibody (4G4) | Invitrogen MA3-009 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HSP70 Monoclonal Antibody (4G4)

catalog :

MA3-009

quantity :

50 µL

price :

US 425.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4G4

reactivity :

human, mouse, rhesus macaque

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 18

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:5000	Klaić L, Morimoto R, Silverman R. Celastrol analogues as inducers of the heat shock response. Design and synthesis of affinity probes for the identification of protein targets. ACS Chem Biol. 2012;7:928-37 pubmed publisher
western blot; human; 1:10000	Calamini B, Silva M, Madoux F, Hutt D, Khanna S, Chalfant M, et al. Small-molecule proteostasis regulators for protein conformational diseases. Nat Chem Biol. 2011;8:185-96 pubmed publisher
blocking or activating experiments; rhesus macaque	Gutierrez M, Isa P, Sánchez San Martín C, Pérez Vargas J, Espinosa R, Arias C, et al. Different rotavirus strains enter MA104 cells through different endocytic pathways: the role of clathrin-mediated endocytosis. J Virol. 2010;84:9161-9 pubmed publisher
western blot; human	Pardue S, Wang S, Miller M, Morrison Bogorad M. Elevated levels of inducible heat shock 70 proteins in human brain. Neurobiol Aging. 2007;28:314-24 pubmed
	Beltrán Hernández I, Angelier M, Del Buono D Ondes T, Di Maggio A, Yu Y, Oliveira S. The Potential of Nanobody-Targeted Photodynamic Therapy to Trigger Immune Responses. Cancers (Basel). 2020;12: pubmed publisher
	Liu P, Bao H, Jin C, Zhou J, Hua F, Li K, et al. Targeting Extracellular Heat Shock Protein 70 Ameliorates Doxorubicin-Induced Heart Failure Through Resolution of Toll-Like Receptor 2-Mediated Myocardial Inflammation. J Am Heart Assoc. 2019;8:e012338 pubmed publisher
	Chutipongtanate S, Greis K. Multiplex Biomarker Screening Assay for Urinary Extracellular Vesicles Study: A Targeted Label-Free Proteomic Approach. Sci Rep. 2018;8:15039 pubmed publisher
	Wang J, De Veirman K, De Beule N, Maes K, De Bruyne E, Van Valckenborgh E, et al. The bone marrow microenvironment enhances multiple myeloma progression by exosome-mediated activation of myeloid-derived suppressor cells. Oncotarget. 2015;6:43992-4004 pubmed publisher
	Sashchenko L, Dukhanina E, Shatalov Y, Yashin D, Lukyanova T, Kabanova O, et al. Cytotoxic T lymphocytes carrying a pattern recognition protein Tag7 can detect evasive, HLA-negative but Hsp70-exposing tumor cells, thereby ensuring FasL/Fas-mediated contact killing. Blood. 2007;110:1997-2004 pubmed
	Westerheide S, Bosman J, Mbadugha B, Kawahara T, Matsumoto G, Kim S, et al. Celastrols as inducers of the heat shock response and cytoprotection. J Biol Chem. 2004;279:56053-60 pubmed
	Alastalo T, Hellesuo M, Sandqvist A, Hietakangas V, Kallio M, Sistonen L. Formation of nuclear stress granules involves HSF2 and coincides with the nucleolar localization of Hsp70. J Cell Sci. 2003;116:3557-70 pubmed
	Barnes J, Dix D, Collins B, Luft C, Allen J. Expression of inducible Hsp70 enhances the proliferation of MCF-7 breast cancer cells and protects against the cytotoxic effects of hyperthermia. Cell Stress Chaperones. 2001;6:316-25 pubmed
	Fishelson Z, Hochman I, Greene L, Eisenberg E. Contribution of heat shock proteins to cell protection from complement-mediated lysis. Int Immunol. 2001;13:983-91 pubmed
	Pirkkala L, Alastalo T, Nykanen P, Seppa L, Sistonen L. Differentiation lineage-specific expression of human heat shock transcription factor 2. FASEB J. 1999;13:1089-98 pubmed
	Botzler C, Li G, Issels R, Multhoff G. Definition of extracellular localized epitopes of Hsp70 involved in an NK immune response. Cell Stress Chaperones. 1998;3:6-11 pubmed
	Suzuki K, Sawa Y, Kaneda Y, Ichihara H, Shirakura R, Matsuda H. [In vivo gene transfection with heat shock protein 70 enhances myocardial tolerance to ischemia-reperfusion injury in rat]. J Cardiol. 1998;31:187-8 pubmed
	Leppa S, Pirkkala L, Chow S, Eriksson J, Sistonen L. Thioredoxin is transcriptionally induced upon activation of heat shock factor 2. J Biol Chem. 1997;272:30400-4 pubmed
	Leppa S, Pirkkala L, Saarento H, Sarge K, Sistonen L. Overexpression of HSF2-beta inhibits hemin-induced heat shock gene expression and erythroid differentiation in K562 cells. J Biol Chem. 1997;272:15293-8 pubmed

product information

Product Type :

Antibody

Product Name :

HSP70 Monoclonal Antibody (4G4)

Catalog # :

MA3-009

Quantity :

50 µL

Price :

US 425.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

Flow Cytometry: 1:100 - 1:200, Immunocytochemistry: 1:100, Immunohistochemistry: 1:20 - 50, Immunoprecipitation: 1:1,000, Inhibition Assays: Assay-dependent, Western Blot: 1:500

Species :

Human, Mouse

Clone :

4G4

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Immunogen :

Human recombinant HSP70 over expressed in E. coli.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1:100 - 1:200, Immunocytochemistry: 1:100, Immunohistochemistry: 1:20 - 50, Immunoprecipitation: 1:1,000, Inhibition Assays: Assay-dependent, Western Blot: 1:500

Aliases :

68 kDa heat shock protein; 70 kda heat shock protein-2; 70 kilodalton heat shock protein; 87A; 87A locus; 87C locus; Bb; CG18743; CG31359; CG31359-PA; CG31366; CG31366-PA; CG31449; CG6489; chaperone; dHSP70; dhsp70Aa; Dmel CG31359; Dmel CG31366; Dmel_CG31359; Dmel_CG31366; Dm-hsp70; DmHSP70AA; DMHSP7D1; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; Hcp70.1; heat shock 10kDa protein 1-like; heat shock 70; heat shock 70 kD protein 1; heat shock 70 kD protein 2; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1-Hom; Heat shock 70 kDa protein 1L; heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; heat shock 70 kDa-like protein 1; heat shock 70kD protein 1A; heat shock 70kD protein 1B; heat shock 70kD protein 1B (mapped); heat shock 70kD protein-like 1; heat shock 70kDa protein 1A; heat shock 70kDa protein 1B; heat shock 70kDa protein 1-like; heat shock cognate 70-kd protein, tandem duplicate 2; Heat shock protein; heat shock protein 1A; heat shock protein 1B; heat shock protein 1-like; heat shock protein 70; heat shock protein 70.1; heat shock protein 70.2; heat shock protein 70-1; Heat shock protein 70-1l; Heat shock protein 70Aa; Heat shock protein 70Ab; Heat shock protein 70Ba; Heat shock protein 70Bb; Heat shock protein 70Bc; heat shock protein cognate 70, testis; heat shock protein family A (Hsp70) member 1 like; heat shock protein family A (Hsp70) member 1A; heat shock protein family A (Hsp70) member 1B; heat shock protein, 70 kDa 1; heat shock protein, 70 kDa 3; heat shock protein-70; heat shock-induced protein; heat shock-related protein hsc70t; heat-shock 70-kilodalton protein 1A; heat-shock 70-kilodalton protein 1B; heat-shock protein 70; Heat-shock-protein-70Aa; Heat-shock-protein-70Bb; HEL S 103; HEL-S-103; hs70; Hsc70t; HSP; Hsp 70; hsp68; Hsp70; Hsp-70; HSP70 1; HSP70 1B; HSP70 2; hsp70 87A; hsp70 87C; hsp70 Aa; HSP70 AceyltK77; hsp70(87A); Hsp70(87C); HSP70.1; HSP70.1/2; HSP70.1/HSP70.2; HSP70.2; Hsp70.3; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-1B; HSP70-1L; HSP70-2; Hsp70-3; HSP70-87A7; HSP70-87C1; hsp70a; hsp70A1; Hsp70A7d; Hsp70Aa; Hsp-70Aa; Hsp70Aa-PA; Hsp70Ab; Hsp70B; Hsp70Ba; Hsp70Bb; Hsp70Bbc; Hsp70Bb-PA; Hsp70Bc; HSP70-HOM; HSP70I; HSP70T; HSP72; Hspa1; Hspa1a; HSPA1B; Hspa1l; Hspa2; Hspa70; HSX70; hum70t; inducible heat shock protein 70; LP05203p; LP05233p; LP08776p; Major heat shock 70 kDa protein Aa; Major heat shock 70 kDa protein Ab; Major heat shock 70 kDa protein Ba; Major heat shock 70 kDa protein Bb; Major heat shock 70 kDa protein Bc; Msh5; protein 70; si:ch73-46h18.6; si:dkeyp-69e1.1; Spermatid-specific heat shock protein 70; zgc:174006

more info or order :

Invitrogen product webpage