HSP70 Monoclonal Antibody (5A5) | Invitrogen MA3-007 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HSP70 Monoclonal Antibody (5A5)

catalog :

MA3-007

quantity :

250 µL

price :

US 425.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5A5

reactivity :

California mussel, forest day mosquito, scallops, African green monkey, Pacific oyster, Lycaena tityrus, hamsters, human, mouse, rat, dogs, fruit fly

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 49

Published Application/Species/Sample/Dilution	Reference
western blot; scallops; fig 7	Ivanina A, Nesmelova I, Leamy L, Sokolov E, Sokolova I. Intermittent hypoxia leads to functional reorganization of mitochondria and affects cellular bioenergetics in marine molluscs. J Exp Biol. 2016;219:1659-74 pubmed publisher
western blot; human; fig 1	Mutschelknaus L, Peters C, Winkler K, Yentrapalli R, Heider T, Atkinson M, et al. Exosomes Derived from Squamous Head and Neck Cancer Promote Cell Survival after Ionizing Radiation. PLoS ONE. 2016;11:e0152213 pubmed publisher
western blot; mouse	Azimzadeh O, Sievert W, Sarioglu H, Merl Pham J, Yentrapalli R, Bakshi M, et al. Integrative proteomics and targeted transcriptomics analyses in cardiac endothelial cells unravel mechanisms of long-term radiation-induced vascular dysfunction. J Proteome Res. 2015;14:1203-19 pubmed publisher
ELISA; fruit fly ; 1:1000; fig 3	Calabria G, Dolgova O, Rego C, Castañeda L, Rezende E, Balanya J, et al. Hsp70 protein levels and thermotolerance in Drosophila subobscura: a reassessment of the thermal co-adaptation hypothesis. J Evol Biol. 2012;25:691-700 pubmed publisher
western blot; mouse; 1:2000	Marshall D, Dong Y, McQuaid C, Williams G. Thermal adaptation in the intertidal snail Echinolittorina malaccana contradicts current theory by revealing the crucial roles of resting metabolism. J Exp Biol. 2011;214:3649-57 pubmed publisher
western blot; mouse	Noack R, Frede S, Albrecht P, Henke N, Pfeiffer A, Knoll K, et al. Charcot-Marie-Tooth disease CMT4A: GDAP1 increases cellular glutathione and the mitochondrial membrane potential. Hum Mol Genet. 2012;21:150-62 pubmed publisher
western blot; mouse; 1:1000	Bivi N, Picotti P, Müller L, Romanello M, Moro L, Quadrifoglio F, et al. Shotgun proteomics analysis reveals new unsuspected molecular effectors of nitrogen-containing bisphosphonates in osteocytes. J Proteomics. 2011;74:1113-22 pubmed publisher
ELISA; Lycaena tityrus; 1:700; loading ...; fig 2c	Fischer K, Kölzow N, Höltje H, Karl I. Assay conditions in laboratory experiments: is the use of constant rather than fluctuating temperatures justified when investigating temperature-induced plasticity?. Oecologia. 2011;166:23-33 pubmed publisher
immunohistochemistry; fruit fly ; 1:200	Chen H, Anagnostou G, Chai A, Withers J, Morris A, Adhikaree J, et al. Characterization of the properties of a novel mutation in VAPB in familial amyotrophic lateral sclerosis. J Biol Chem. 2010;285:40266-81 pubmed publisher
western blot; fruit fly	Juge F, Fernando C, Fic W, Tazi J. The SR protein B52/SRp55 is required for DNA topoisomerase I recruitment to chromatin, mRNA release and transcription shutdown. PLoS Genet. 2010;6:e1001124 pubmed publisher
western blot; California mussel; 1:1000	Urian A, Hatle J, Gilg M. Thermal constraints for range expansion of the invasive green mussel, Perna viridis, in the southeastern United States. J Exp Zool A Ecol Genet Physiol. 2011;315:12-21 pubmed publisher
immunocytochemistry; mouse; 1:3000	Cho K, Yi H, Tserentsoodol N, Searle K, Ferreira P. Neuroprotection resulting from insufficiency of RANBP2 is associated with the modulation of protein and lipid homeostasis of functionally diverse but linked pathways in response to oxidative stress. Dis Model Mech. 2010;3:595-604 pubmed publisher
western blot; mouse	Rozich R, Mills D, Brilliant K, Callanan H, Yang D, Tantravahi U, et al. Accumulation of neoplastic traits prior to spontaneous in vitro transformation of rat cholangiocytes determines susceptibility to activated ErbB-2/Neu. Exp Mol Pathol. 2010;89:248-59 pubmed publisher
immunocytochemistry; mouse; 1:50	Paoletti F, Ainger K, Donati I, Scardigli R, Vetere A, Cattaneo A, et al. Novel fluorescent cycloheximide derivatives for the imaging of protein synthesis. Biochem Biophys Res Commun. 2010;396:258-64 pubmed publisher
immunohistochemistry; fruit fly ; 1:500	Nisoli I, Chauvin J, Napoletano F, Calamita P, Zanin V, Fanto M, et al. Neurodegeneration by polyglutamine Atrophin is not rescued by induction of autophagy. Cell Death Differ. 2010;17:1577-87 pubmed publisher
western blot; human	Viteri G, Chung Y, Stadtman E. Effect of progerin on the accumulation of oxidized proteins in fibroblasts from Hutchinson Gilford progeria patients. Mech Ageing Dev. 2010;131:2-8 pubmed publisher
western blot; mouse	Wong S, Leung P, Djurisić A, Leung K. Toxicities of nano zinc oxide to five marine organisms: influences of aggregate size and ion solubility. Anal Bioanal Chem. 2010;396:609-18 pubmed publisher
western blot; mouse	Yuan X, Chen L, Li M, Dong P, Xue J, Wang J, et al. Elevated expression of Foxp3 in tumor-infiltrating Treg cells suppresses T-cell proliferation and contributes to gastric cancer progression in a COX-2-dependent manner. Clin Immunol. 2010;134:277-88 pubmed publisher
immunohistochemistry; human; 1:150 western blot; human	Codarin E, Renzone G, Poz A, Avellini C, Baccarani U, Lupo F, et al. Differential proteomic analysis of subfractioned human hepatocellular carcinoma tissues. J Proteome Res. 2009;8:2273-84 pubmed publisher
western blot; mouse	Tani F, Ohno M, Furukawa Y, Sakamoto M, Masuda S, Kitabatake N. Surface expression of a C-terminal alpha-helix region in heat shock protein 72 on murine LL/2 lung carcinoma can be recognized by innate immune sentinels. Mol Immunol. 2009;46:1326-39 pubmed publisher
western blot; Pacific oyster	Ivanina A, Taylor C, Sokolova I. Effects of elevated temperature and cadmium exposure on stress protein response in eastern oysters Crassostrea virginica (Gmelin). Aquat Toxicol. 2009;91:245-54 pubmed publisher
flow cytometry; human western blot; human	Chavez Salinas S, Ceballos Olvera I, Reyes del Valle J, Medina F, Del Angel R. Heat shock effect upon dengue virus replication into U937 cells. Virus Res. 2008;138:111-8 pubmed publisher
western blot; Pacific oyster	Ivanina A, Sokolova I, Sukhotin A. Oxidative stress and expression of chaperones in aging mollusks. Comp Biochem Physiol B Biochem Mol Biol. 2008;150:53-61 pubmed publisher
western blot; Pacific oyster	Ivanina A, Cherkasov A, Sokolova I. Effects of cadmium on cellular protein and glutathione synthesis and expression of stress proteins in eastern oysters, Crassostrea virginica Gmelin. J Exp Biol. 2008;211:577-86 pubmed publisher
western blot; forest day mosquito; 1:5,000 western blot; hamsters; 1:5,000	Salas Benito J, Reyes del Valle J, Salas Benito M, Ceballos Olvera I, Mosso C, Del Angel R. Evidence that the 45-kD glycoprotein, part of a putative dengue virus receptor complex in the mosquito cell line C6/36, is a heat-shock related protein. Am J Trop Med Hyg. 2007;77:283-90 pubmed
blocking or activating experiments; African green monkey; 1:100 blocking or activating experiments; hamsters; 1:100	López T, Lopez S, Arias C. Heat shock enhances the susceptibility of BHK cells to rotavirus infection through the facilitation of entry and post-entry virus replication steps. Virus Res. 2006;121:74-83 pubmed
immunohistochemistry; fruit fly ; 1:100	Chartier A, Benoit B, Simonelig M. A Drosophila model of oculopharyngeal muscular dystrophy reveals intrinsic toxicity of PABPN1. EMBO J. 2006;25:2253-62 pubmed
western blot; mouse	Klose M, Chu D, Xiao C, Seroude L, Robertson R. Heat shock-mediated thermoprotection of larval locomotion compromised by ubiquitous overexpression of Hsp70 in Drosophila melanogaster. J Neurophysiol. 2005;94:3563-72 pubmed
western blot; fruit fly ; 1:1000	Newman A, Xiao C, Robertson R. Synaptic thermoprotection in a desert-dwelling Drosophila species. J Neurobiol. 2005;64:170-80 pubmed
	Xiao C, Hull D, Qiu S, Yeung J, Zheng J, Barwell T, et al. Expression of Heat Shock Protein 70 Is Insufficient To Extend Drosophila melanogaster Longevity. G3 (Bethesda). 2019;9:4197-4207 pubmed publisher
	Attwood K, Fleyshman D, Prendergast L, Paszkiewicz G, Omilian A, Bshara W, et al. Prognostic value of histone chaperone FACT subunits expression in breast cancer. Breast Cancer (Dove Med Press). 2017;9:301-311 pubmed publisher
	Horikawa I, Fujita K, Jenkins L, Hiyoshi Y, Mondal A, Vojtesek B, et al. Autophagic degradation of the inhibitory p53 isoform ?133p53? as a regulatory mechanism for p53-mediated senescence. Nat Commun. 2014;5:4706 pubmed publisher
	Wong S, Leung K. Temperature-dependent toxicities of nano zinc oxide to marine diatom, amphipod and fish in relation to its aggregation size and ion dissolution. Nanotoxicology. 2014;8 Suppl 1:24-35 pubmed publisher
	Wang L, Baruah K, Fan T, Yu M, Bossier P. Influence of heat shock proteins induction in different yeast cell wall mutants on the protection against Vibrio campbellii infection in gnotobiotically grown Artemia franciscana (Kellogg). J Fish Dis. 2010;33:919-23 pubmed
	Calvert M, Digilio L, Herr J, Coonrod S. Oolemmal proteomics--identification of highly abundant heat shock proteins and molecular chaperones in the mature mouse egg and their localization on the plasma membrane. Reprod Biol Endocrinol. 2003;1:27 pubmed
	Fishelson Z, Hochman I, Greene L, Eisenberg E. Contribution of heat shock proteins to cell protection from complement-mediated lysis. Int Immunol. 2001;13:983-91 pubmed
	Giraldo R, Diaz Orejas R. Similarities between the DNA replication initiators of Gram-negative bacteria plasmids (RepA) and eukaryotes (Orc4p)/archaea (Cdc6p). Proc Natl Acad Sci U S A. 2001;98:4938-43 pubmed
	Thress K, Song J, Morimoto R, Kornbluth S. Reversible inhibition of Hsp70 chaperone function by Scythe and Reaper. EMBO J. 2001;20:1033-41 pubmed
	de Haan P, Vanicky I, Jacobs M, Bakker O, Lips J, Meylaerts S, et al. Effect of ischemic pretreatment on heat shock protein 72, neurologic outcome, and histopathologic outcome in a rabbit model of spinal cord ischemia. J Thorac Cardiovasc Surg. 2000;120:513-9 pubmed
	Bonner J, Carlson T, Fackenthal D, Paddock D, Storey K, Lea K. Complex regulation of the yeast heat shock transcription factor. Mol Biol Cell. 2000;11:1739-51 pubmed
	Kakeya H, Udono H, Maesaki S, Sasaki E, Kawamura S, Hossain M, et al. Heat shock protein 70 (hsp70) as a major target of the antibody response in patients with pulmonary cryptococcosis. Clin Exp Immunol. 1999;115:485-90 pubmed
	Botzler C, Li G, Issels R, Multhoff G. Definition of extracellular localized epitopes of Hsp70 involved in an NK immune response. Cell Stress Chaperones. 1998;3:6-11 pubmed
	Herbertsson H, Kühme T, Evertsson U, Wigren J, Hammarstrom S. Identification of subunits of the 650 kDa 12(S)-HETE binding complex in carcinoma cells. J Lipid Res. 1998;39:237-44 pubmed
	Gabai V, Meriin A, Mosser D, Caron A, Rits S, Shifrin V, et al. Hsp70 prevents activation of stress kinases. A novel pathway of cellular thermotolerance. J Biol Chem. 1997;272:18033-7 pubmed
	van den Berg J, Smets L, Van Rooij H. Agonist-free transformation of the glucocorticoid receptor in human B-lymphoma cells. J Steroid Biochem Mol Biol. 1996;57:239-49 pubmed
	Richter D, Harn D. Candidate vaccine antigens identified by antibodies from mice vaccinated with 15- or 50-kilorad-irradiated cercariae of Schistosoma mansoni. Infect Immun. 1993;61:146-54 pubmed
	Lithgow T, Ryan M, Anderson R, Høj P, Hoogenraad N. A constitutive form of heat-shock protein 70 is located in the outer membranes of mitochondria from rat liver. FEBS Lett. 1993;332:277-81 pubmed
	Mifflin L, Cohen R. hsc70 moderates the heat shock (stress) response in Xenopus laevis oocytes and binds to denatured protein inducers. J Biol Chem. 1994;269:15718-23 pubmed
	Wheeler J, Bieschke E, Tower J. Muscle-specific expression of Drosophila hsp70 in response to aging and oxidative stress. Proc Natl Acad Sci U S A. 1995;92:10408-12 pubmed

image

image 1 :

Western blot analysis of Heat Shock Protein 70 (HSP70) was performed by loading 50µg of the indicated whole cell lysates and 15?l of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP70 monoclonal antibody (Product # MA3-007) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

image 2 :

Immunofluorescent analysis of Heat Shock Protein 70 using Heat Shock Protein 70 Monoclonal antibody (5A5) (Product# MA3-007) shows staining in HeLa cells. Heat Shock Protein 70 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 70 (Product# MA3-007) at a dilution of 1:100-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

image 3 :

Immunohistochemistry was performed on cancer biopsies of deparaffinized Human prostate carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Heat Shock Protein 70 (MA3-007) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

product information

Product Type :

Antibody

Product Name :

HSP70 Monoclonal Antibody (5A5)

Catalog # :

MA3-007

Quantity :

250 µL

Price :

US 425.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Amphibian, Avian, Canine, Drosophila, Fish, Flatworm, Human, Mouse, Non-human primate, Rat, Yeast

Applications :

Gel Shift: Assay-dependent, Immunocytochemistry: 1:200, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:250-1:1,000

Species :

Amphibian, Avian, Canine, Drosophila, Fish, Flatworm, Human, Mouse, Non-human primate, Rat, Yeast

Clone :

5A5

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Immunogen :

Human recombinant HSP70 over expressed in E. coli.

Format :

Liquid

Applications w/Dilutions :

Gel Shift: Assay-dependent, Immunocytochemistry: 1:200, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:250-1:1,000

Aliases :

68 kDa heat shock protein; 70 kda heat shock protein-2; 70 kilodalton heat shock protein; 87A; 87A locus; 87C locus; Bb; CG18743; CG31359; CG31359-PA; CG31366; CG31366-PA; CG31449; CG6489; chaperone; dHSP70; dhsp70Aa; Dmel CG31359; Dmel CG31366; Dmel_CG31359; Dmel_CG31366; Dm-hsp70; DmHSP70AA; DMHSP7D1; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; Hcp70.1; heat shock 10kDa protein 1-like; heat shock 70; heat shock 70 kD protein 1; heat shock 70 kD protein 2; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1-Hom; Heat shock 70 kDa protein 1L; heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; heat shock 70 kDa-like protein 1; heat shock 70kD protein 1A; heat shock 70kD protein 1B; heat shock 70kD protein 1B (mapped); heat shock 70kD protein-like 1; heat shock 70kDa protein 1A; heat shock 70kDa protein 1B; heat shock 70kDa protein 1-like; heat shock cognate 70-kd protein, tandem duplicate 2; Heat shock protein; heat shock protein 1A; heat shock protein 1B; heat shock protein 1-like; heat shock protein 70; heat shock protein 70.1; heat shock protein 70.2; heat shock protein 70-1; Heat shock protein 70-1l; Heat shock protein 70Aa; Heat shock protein 70Ab; Heat shock protein 70Ba; Heat shock protein 70Bb; Heat shock protein 70Bc; heat shock protein cognate 70, testis; heat shock protein family A (Hsp70) member 1 like; heat shock protein family A (Hsp70) member 1A; heat shock protein family A (Hsp70) member 1B; heat shock protein, 70 kDa 1; heat shock protein, 70 kDa 3; heat shock protein-70; heat shock-induced protein; heat shock-related protein hsc70t; heat-shock 70-kilodalton protein 1A; heat-shock 70-kilodalton protein 1B; heat-shock protein 70; Heat-shock-protein-70Aa; Heat-shock-protein-70Bb; HEL S 103; HEL-S-103; hs70; Hsc70t; HSP; Hsp 70; hsp68; Hsp70; Hsp-70; HSP70 1; HSP70 1B; HSP70 2; hsp70 87A; hsp70 87C; hsp70 Aa; HSP70 AceyltK77; hsp70(87A); Hsp70(87C); HSP70.1; HSP70.1/2; HSP70.1/HSP70.2; HSP70.2; Hsp70.3; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-1B; HSP70-1L; HSP70-2; Hsp70-3; HSP70-87A7; HSP70-87C1; hsp70a; hsp70A1; Hsp70A7d; Hsp70Aa; Hsp-70Aa; Hsp70Aa-PA; Hsp70Ab; Hsp70B; Hsp70Ba; Hsp70Bb; Hsp70Bbc; Hsp70Bb-PA; Hsp70Bc; HSP70-HOM; HSP70I; HSP70T; HSP72; Hspa1; Hspa1a; HSPA1B; Hspa1l; Hspa2; Hspa70; HSX70; hum70t; inducible heat shock protein 70; LP05203p; LP05233p; LP08776p; Major heat shock 70 kDa protein Aa; Major heat shock 70 kDa protein Ab; Major heat shock 70 kDa protein Ba; Major heat shock 70 kDa protein Bb; Major heat shock 70 kDa protein Bc; Msh5; protein 70; si:ch73-46h18.6; si:dkeyp-69e1.1; Spermatid-specific heat shock protein 70; zgc:174006

more info or order :

Invitrogen product webpage