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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
HSP70 Monoclonal Antibody (3A3)
catalog :
MA3-006
quantity :
50 µg
price :
US 425.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3A3
reactivity :
Artemia franciscana, Chlamydomonas reinhardtii, common limpet, roach minnow, Eyprepocnemis plorans, eastern oyster, Asian green mussel, lobsters, red rock crab, Marenzelleria viridis, giant tiger prawn, holothurians, Japanese rice fish, sea monkeys, Saccharomycetales, Pacific oyster, Meganyctiphanes norvegica, snow fleas, crucian carp, human, mouse, rat, dogs, pigs , fruit fly , roundworm , rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, blocking or activating experiments
more info or order :
Invitrogen product webpage
citations: 84
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:200; loading ...; fig 5
Solárová Z, Kello M, Varinska L, Budovská M, Solar P. Inhibition of heat shock protein (Hsp) 90 potentiates the antiproliferative and pro-apoptotic effects of 2-(4'fluoro-phenylamino)-4H-1,3-thiazine[6,5-b]indole in A2780cis cells. Biomed Pharmacother. 2017;85:463-471 pubmed publisher
  • western blot; Artemia franciscana; 1:5000; fig 2a
  • western blot; human; 1:5000
Pestana J, Novais S, Norouzitallab P, Vandegehuchte M, Bossier P, De Schamphelaere K. Non-lethal heat shock increases tolerance to metal exposure in brine shrimp. Environ Res. 2016;151:663-670 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 3c
Sclip A, Bacaj T, Giam L, Sudhof T. Extended Synaptotagmin (ESyt) Triple Knock-Out Mice Are Viable and Fertile without Obvious Endoplasmic Reticulum Dysfunction. PLoS ONE. 2016;11:e0158295 pubmed publisher
  • western blot; Pacific oyster; 1:2500; fig 1
Yang C, Sierp M, Abbott C, Li Y, Qin J. Responses to thermal and salinity stress in wild and farmed Pacific oysters Crassostrea gigas. Comp Biochem Physiol A Mol Integr Physiol. 2016;201:22-29 pubmed publisher
  • western blot; mouse; 1:5000; fig 1
Schachtner H, Weimershaus M, Stache V, Plewa N, Legler D, Höpken U, et al. Loss of Gadkin Affects Dendritic Cell Migration In Vitro. PLoS ONE. 2015;10:e0143883 pubmed publisher
  • western blot; common limpet; 1:1000
Lima F, Gomes F, Seabra R, Wethey D, Seabra M, Cruz T, et al. Loss of thermal refugia near equatorial range limits. Glob Chang Biol. 2016;22:254-63 pubmed publisher
  • western blot; Asian green mussel; 1:5000; fig 2
Aleng N, Sung Y, MacRae T, Abd Wahid M. Non-Lethal Heat Shock of the Asian Green Mussel, Perna viridis, Promotes Hsp70 Synthesis, Induces Thermotolerance and Protects Against Vibrio Infection. PLoS ONE. 2015;10:e0135603 pubmed publisher
  • immunohistochemistry; holothurians; 1:200
  • western blot; holothurians; 1:1000
Xu D, Sun L, Liu S, Zhang L, Yang H. Histological, ultrastructural and heat shock protein 70 (HSP70) responses to heat stress in the sea cucumber Apostichopus japonicus. Fish Shellfish Immunol. 2015;45:321-6 pubmed publisher
  • western blot; mouse; fig 2
Cho O, Mallappa C, Hernández Hernández J, Rivera Pérez J, Imbalzano A. Contrasting roles for MyoD in organizing myogenic promoter structures during embryonic skeletal muscle development. Dev Dyn. 2015;244:43-55 pubmed publisher
  • immunocytochemistry; mouse
Meakin C, Qin J, Pogson L, Abbott C. Thermal tolerance in juvenile King George whiting (Sillaginodes punctata) reduces as fish age and this reduction coincides with migration to deeper colder water. Comp Biochem Physiol A Mol Integr Physiol. 2014;172:46-51 pubmed publisher
  • western blot; Meganyctiphanes norvegica; 1:5000
Loc N, MacRae T, Musa N, Bin Abdullah M, Abdul Wahid M, Sung Y. Non-lethal heat shock increased Hsp70 and immune protein transcripts but not Vibrio tolerance in the white-leg shrimp. PLoS ONE. 2013;8:e73199 pubmed publisher
  • western blot; Japanese rice fish; 1:5000
Sung Y, Roberts R, Bossier P. Enhancement of Hsp70 synthesis protects common carp, Cyprinus carpio L., against lethal ammonia toxicity. J Fish Dis. 2012;35:563-8 pubmed publisher
  • western blot; human
Rueckert C, Haucke V. The oncogenic TBC domain protein USP6/TRE17 regulates cell migration and cytokinesis. Biol Cell. 2012;104:22-33 pubmed publisher
  • immunohistochemistry; Japanese rice fish; 1:1000
  • western blot; Japanese rice fish; 1:5000
Vehniäinen E, Vahakangas K, Oikari A. UV-B exposure causes DNA damage and changes in protein expression in northern pike (Esox lucius) posthatched embryos. Photochem Photobiol. 2012;88:363-70 pubmed publisher
  • western blot; giant tiger prawn; 1:1000
Dorts J, Silvestre F, Tu H, Tyberghein A, Phuong N, Kestemont P. Oxidative stress, protein carbonylation and heat shock proteins in the black tiger shrimp, Penaeus monodon, following exposure to endosulfan and deltamethrin. Environ Toxicol Pharmacol. 2009;28:302-10 pubmed publisher
  • western blot; human
Baruah K, Ranjan J, Sorgeloos P, MacRae T, Bossier P. Priming the prophenoloxidase system of Artemia franciscana by heat shock proteins protects against Vibrio campbellii challenge. Fish Shellfish Immunol. 2011;31:134-41 pubmed publisher
  • western blot; Japanese rice fish
Oikari A, Lahti M, Meriläinen P, Afanasyev S, Krasnov A. Do historical sediments of pulp and paper industry contribute to the exposure of fish caged in receiving waters?. J Environ Monit. 2010;12:1045-54 pubmed
  • western blot; Japanese rice fish; 1:1000
Douxfils J, Mandiki S, Marotte G, Wang N, Silvestre F, Milla S, et al. Does domestication process affect stress response in juvenile Eurasian perch Perca fluviatilis?. Comp Biochem Physiol A Mol Integr Physiol. 2011;159:92-9 pubmed publisher
  • western blot; Eyprepocnemis plorans
Teruel M, Sørensen J, Loeschcke V, Cabrero J, Perfectti F, Camacho J. Level of heat shock proteins decreases in individuals carrying B-chromosomes in the grasshopper Eyprepocnemis plorans. Cytogenet Genome Res. 2011;132:94-9 pubmed publisher
  • western blot; human
Kuliková L, Mikes J, Hyzdalova M, Palumbo G, Fedorocko P. NF-?B is not directly responsible for photoresistance induced by fractionated light delivery in HT-29 colon adenocarcinoma cells. Photochem Photobiol. 2010;86:1285-93 pubmed publisher
  • western blot; lobsters; 1:5000
Baruah K, Ranjan J, Sorgeloos P, Bossier P. Efficacy of heterologous and homologous heat shock protein 70s as protective agents to Artemia franciscana challenged with Vibrio campbellii. Fish Shellfish Immunol. 2010;29:733-9 pubmed publisher
  • blocking or activating experiments; rhesus macaque
Gutierrez M, Isa P, Sánchez San Martín C, Pérez Vargas J, Espinosa R, Arias C, et al. Different rotavirus strains enter MA104 cells through different endocytic pathways: the role of clathrin-mediated endocytosis. J Virol. 2010;84:9161-9 pubmed publisher
  • western blot; human; 1:1000
Mikes J, Kovaľ J, Jendželovský R, Sacková V, Uhrinová I, Kello M, et al. The role of p53 in the efficiency of photodynamic therapy with hypericin and subsequent long-term survival of colon cancer cells. Photochem Photobiol Sci. 2009;8:1558-67 pubmed publisher
  • western blot; mouse
Tani F, Ohno M, Furukawa Y, Sakamoto M, Masuda S, Kitabatake N. Surface expression of a C-terminal alpha-helix region in heat shock protein 72 on murine LL/2 lung carcinoma can be recognized by innate immune sentinels. Mol Immunol. 2009;46:1326-39 pubmed publisher
  • western blot; snow fleas; 1:5000
Bahrndorff S, Tunnacliffe A, Wise M, McGee B, Holmstrup M, Loeschcke V. Bioinformatics and protein expression analyses implicate LEA proteins in the drought response of Collembola. J Insect Physiol. 2009;55:210-7 pubmed publisher
  • western blot; Pacific oyster; 1:3000
Meistertzheim A, Lejart M, Le Goïc N, Thébault M. Sex-, gametogenesis, and tidal height-related differences in levels of HSP70 and metallothioneins in the Pacific oyster Crassostrea gigas. Comp Biochem Physiol A Mol Integr Physiol. 2009;152:234-9 pubmed publisher
  • western blot; roach minnow; 1:5000
Korkea aho T, Vehniäinen E, Kukkonen J, Taskinen J. The effects of treated effluents on the intensity of papillomatosis and HSP70 expression in roach. Ecotoxicol Environ Saf. 2008;70:462-8 pubmed publisher
  • immunoprecipitation; mouse
Taherian A, Krone P, Ovsenek N. A comparison of Hsp90alpha and Hsp90beta interactions with cochaperones and substrates. Biochem Cell Biol. 2008;86:37-45 pubmed publisher
  • western blot; Pacific oyster
Li Y, Qin J, Abbott C, Li X, Benkendorff K. Synergistic impacts of heat shock and spawning on the physiology and immune health of Crassostrea gigas: an explanation for summer mortality in Pacific oysters. Am J Physiol Regul Integr Comp Physiol. 2007;293:R2353-62 pubmed
  • western blot; eastern oyster; 1:5000
Encomio V, Chu F. Heat shock protein (hsp70) expression and thermal tolerance in sublethally heat-shocked eastern oysters Crassostrea virginica infected with the parasite Perkinsus marinus. Dis Aquat Organ. 2007;76:251-60 pubmed
  • western blot; Saccharomycetales; 1:1000
Vianna C, Silva C, Neves M, Rosa C. Saccharomyces cerevisiae strains from traditional fermentations of Brazilian cachaça: trehalose metabolism, heat and ethanol resistance. Antonie Van Leeuwenhoek. 2008;93:205-17 pubmed
  • western blot; red rock crab
Silvestre F, Trausch G, Devos P. Is HSP70 involved in acclimation to cadmium in the Chinese crab, Eriocheir sinensis ?. Bull Environ Contam Toxicol. 2007;78:432-5 pubmed
  • western blot; sea monkeys; 1:5000
Yik Sung Y, Van Damme E, Sorgeloos P, Bossier P. Non-lethal heat shock protects gnotobiotic Artemia franciscana larvae against virulent Vibrios. Fish Shellfish Immunol. 2007;22:318-26 pubmed
  • western blot; Marenzelleria viridis; 1:3000; fig 7a
  • western blot; human; 1:3000
Blank M, Bastrop R, Jürss K. Stress protein response in two sibling species of Marenzelleria (Polychaeta: Spionidae): is there an influence of acclimation salinity?. Comp Biochem Physiol B Biochem Mol Biol. 2006;144:451-62 pubmed
  • western blot; human; 1:5000
Mayon N, Bertrand A, Leroy D, Malbrouck C, Mandiki S, Silvestre F, et al. Multiscale approach of fish responses to different types of environmental contaminations: a case study. Sci Total Environ. 2006;367:715-31 pubmed
  • western blot; fruit fly ; 1:10,000
Neal S, Karunanithi S, Best A, So A, Tanguay R, Atwood H, et al. Thermoprotection of synaptic transmission in a Drosophila heat shock factor mutant is accompanied by increased expression of Hsp83 and DnaJ-1. Physiol Genomics. 2006;25:493-501 pubmed
  • western blot; crucian carp; 1:10000
Rissanen E, Tranberg H, Sollid J, Nilsson G, Nikinmaa M. Temperature regulates hypoxia-inducible factor-1 (HIF-1) in a poikilothermic vertebrate, crucian carp (Carassius carassius). J Exp Biol. 2006;209:994-1003 pubmed
  • western blot; rat; loading ...; fig 2a
Krauss M, Haucke V. Functional assay of effectors of ADP ribosylation factor 6 during clathrin/AP-2 coat recruitment to membranes. Methods Enzymol. 2005;404:388-98 pubmed
  • western blot; Chlamydomonas reinhardtii
Yang C, Compton M, Yang P. Dimeric novel HSP40 is incorporated into the radial spoke complex during the assembly process in flagella. Mol Biol Cell. 2005;16:637-48 pubmed
Lant J, Berg M, Sze D, Hoffman K, Akinpelu I, Turk M, et al. Visualizing tRNA-dependent mistranslation in human cells. RNA Biol. 2018;15:567-575 pubmed publisher
Rahlff J, Peters J, Moyano M, Pless O, Claussen C, Peck M. Short-term molecular and physiological responses to heat stress in neritic copepods Acartia tonsa and Eurytemora affinis. Comp Biochem Physiol A Mol Integr Physiol. 2017;203:348-358 pubmed publisher
Schmitz M, Douxfils J, Mandiki S, Morana C, Baekelandt S, Kestemont P. Chronic hyperosmotic stress interferes with immune homeostasis in striped catfish (Pangasianodon hypophthalmus, S.) and leads to excessive inflammatory response during bacterial infection. Fish Shellfish Immunol. 2016;55:550-8 pubmed publisher
Norouzitallab P, Baruah K, Vandegehuchte M, Van Stappen G, Catania F, Vanden Bussche J, et al. Environmental heat stress induces epigenetic transgenerational inheritance of robustness in parthenogenetic Artemia model. FASEB J. 2014;28:3552-63 pubmed publisher
Stork F, Backor M, Klejdus B, Hedbavny J, Kováčik J. Changes of metal-induced toxicity by H2O 2/NO modulators in Scenedesmus quadricauda (Chlorophyceae). Environ Sci Pollut Res Int. 2013;20:5502-11 pubmed publisher
Yang C, Owen H, Yang P. Dimeric heat shock protein 40 binds radial spokes for generating coupled power strokes and recovery strokes of 9 + 2 flagella. J Cell Biol. 2008;180:403-15 pubmed publisher
Shapiro J, Ingram J, Johnson K. Characterization of a molecular chaperone present in the eukaryotic flagellum. Eukaryot Cell. 2005;4:1591-4 pubmed
Mitchell B, Pedersen L, Feely M, Rosenbaum J, Mitchell D. ATP production in Chlamydomonas reinhardtii flagella by glycolytic enzymes. Mol Biol Cell. 2005;16:4509-18 pubmed
Häkkinen J, Oikari A. A field methodology to study effects of UV radiation on fish larvae. Water Res. 2004;38:2891-7 pubmed
Häkkinen J, Vehniäinen E, Oikari A. High sensitivity of northern pike larvae to UV-B but no UV-photoinduced toxicity of retene. Aquat Toxicol. 2004;66:393-404 pubmed
Stavreva D, Müller W, Hager G, Smith C, McNally J. Rapid glucocorticoid receptor exchange at a promoter is coupled to transcription and regulated by chaperones and proteasomes. Mol Cell Biol. 2004;24:2682-97 pubmed
Sashchenko L, Dukhanina E, Yashin D, Shatalov Y, Romanova E, Korobko E, et al. Peptidoglycan recognition protein tag7 forms a cytotoxic complex with heat shock protein 70 in solution and in lymphocytes. J Biol Chem. 2004;279:2117-24 pubmed
Wang G, Zhang J, Moskophidis D, Mivechi N. Targeted disruption of the heat shock transcription factor (hsf)-2 gene results in increased embryonic lethality, neuronal defects, and reduced spermatogenesis. Genesis. 2003;36:48-61 pubmed
Cruz Rodríguez L, Chu F. Heat-shock protein (HSP70) response in the eastern oyster, Crassostrea virginica, exposed to PAHs sorbed to suspended artificial clay particles and to suspended field contaminated sediments. Aquat Toxicol. 2002;60:157-68 pubmed
Lafarga M, Berciano M, Pena E, Mayo I, Castaño J, Bohmann D, et al. Clastosome: a subtype of nuclear body enriched in 19S and 20S proteasomes, ubiquitin, and protein substrates of proteasome. Mol Biol Cell. 2002;13:2771-82 pubmed
Cimino E, Owens L, Bromage E, Anderson T. A newly developed ELISA showing the effect of environmental stress on levels of hsp86 in Cherax quadricarinatus and Penaeus monodon. Comp Biochem Physiol A Mol Integr Physiol. 2002;132:591-8 pubmed
Huang L, Mivechi N, Moskophidis D. Insights into regulation and function of the major stress-induced hsp70 molecular chaperone in vivo: analysis of mice with targeted gene disruption of the hsp70.1 or hsp70.3 gene. Mol Cell Biol. 2001;21:8575-91 pubmed
King F, Wawrzynow A, Hohfeld J, Zylicz M. Co-chaperones Bag-1, Hop and Hsp40 regulate Hsc70 and Hsp90 interactions with wild-type or mutant p53. EMBO J. 2001;20:6297-305 pubmed
Fishelson Z, Hochman I, Greene L, Eisenberg E. Contribution of heat shock proteins to cell protection from complement-mediated lysis. Int Immunol. 2001;13:983-91 pubmed
Hynes G, Willison K. Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin. J Biol Chem. 2000;275:18985-94 pubmed
Bharadwaj S, Ali A, Ovsenek N. Multiple components of the HSP90 chaperone complex function in regulation of heat shock factor 1 In vivo. Mol Cell Biol. 1999;19:8033-41 pubmed
Ballinger C, Connell P, Wu Y, Hu Z, Thompson L, Yin L, et al. Identification of CHIP, a novel tetratricopeptide repeat-containing protein that interacts with heat shock proteins and negatively regulates chaperone functions. Mol Cell Biol. 1999;19:4535-45 pubmed
Bierkens J, Van de Perre W, Maes J. Effect of different environmental variables on the synthesis of Hsp70 in Raphidocelis subcapitata. Comp Biochem Physiol A Mol Integr Physiol. 1998;120:29-34 pubmed
Mathew A, Mathur S, Morimoto R. Heat shock response and protein degradation: regulation of HSF2 by the ubiquitin-proteasome pathway. Mol Cell Biol. 1998;18:5091-8 pubmed
Anton L, Snyder H, Bennink J, Vinitsky A, Orlowski M, Porgador A, et al. Dissociation of proteasomal degradation of biosynthesized viral proteins from generation of MHC class I-associated antigenic peptides. J Immunol. 1998;160:4859-68 pubmed
Botzler C, Li G, Issels R, Multhoff G. Definition of extracellular localized epitopes of Hsp70 involved in an NK immune response. Cell Stress Chaperones. 1998;3:6-11 pubmed
Stephens R. Synthesis and turnover of embryonic sea urchin ciliary proteins during selective inhibition of tubulin synthesis and assembly. Mol Biol Cell. 1997;8:2187-98 pubmed
Fourie A, Hupp T, Lane D, Sang B, Barbosa M, Sambrook J, et al. HSP70 binding sites in the tumor suppressor protein p53. J Biol Chem. 1997;272:19471-9 pubmed
Piperno G, Mead K. Transport of a novel complex in the cytoplasmic matrix of Chlamydomonas flagella. Proc Natl Acad Sci U S A. 1997;94:4457-62 pubmed
Bloch M, Johnson K. Identification of a molecular chaperone in the eukaryotic flagellum and its localization to the site of microtubule assembly. J Cell Sci. 1995;108 ( Pt 11):3541-5 pubmed
Nihei T, Takahashi S, Sagae S, Sato N, Kikuchi K. Protein interaction of retinoblastoma gene product pRb110 with M(r) 73,000 heat shock cognate protein. Cancer Res. 1993;53:1702-5 pubmed
Sarge K, Murphy S, Morimoto R. Activation of heat shock gene transcription by heat shock factor 1 involves oligomerization, acquisition of DNA-binding activity, and nuclear localization and can occur in the absence of stress. Mol Cell Biol. 1993;13:1392-407 pubmed
Agoff S, Hou J, Linzer D, Wu B. Regulation of the human hsp70 promoter by p53. Science. 1993;259:84-7 pubmed
Lithgow T, Ryan M, Anderson R, Høj P, Hoogenraad N. A constitutive form of heat-shock protein 70 is located in the outer membranes of mitochondria from rat liver. FEBS Lett. 1993;332:277-81 pubmed
van Laack R, Faustman C, Sebranek J. Pork quality and the expression of stress protein Hsp 70 in swine. J Anim Sci. 1993;71:2958-64 pubmed
Srinivasan G, Patel N, Thompson E. Heat shock protein is tightly associated with the recombinant human glucocorticoid receptor:glucocorticoid response element complex. Mol Endocrinol. 1994;8:189-96 pubmed
Huang Q, Alvares K, Chu R, Bradfield C, Reddy J. Association of peroxisome proliferator-activated receptor and Hsp72. J Biol Chem. 1994;269:8493-7 pubmed
Arnold C, Wittrup K. The stress response to loss of signal recognition particle function in Saccharomyces cerevisiae. J Biol Chem. 1994;269:30412-8 pubmed
Rabindran S, Wisniewski J, Li L, Li G, Wu C. Interaction between heat shock factor and hsp70 is insufficient to suppress induction of DNA-binding activity in vivo. Mol Cell Biol. 1994;14:6552-60 pubmed
Bhattacharyya T, Karnezis A, Murphy S, Hoang T, Freeman B, Phillips B, et al. Cloning and subcellular localization of human mitochondrial hsp70. J Biol Chem. 1995;270:1705-10 pubmed
Carr V, Murphy S, Morimoto R, Farbman A. Small subclass of rat olfactory neurons with specific bulbar projections is reactive with monoclonal antibodies to the HSP70 heat shock protein. J Comp Neurol. 1994;348:150-60 pubmed
Saitoh H, Dasso M. The RCC1 protein interacts with Ran, RanBP1, hsc70, and a 340-kDa protein in Xenopus extracts. J Biol Chem. 1995;270:10658-63 pubmed
Inoue A, Torigoe T, Sogahata K, Kamiguchi K, Takahashi S, Sawada Y, et al. 70-kDa heat shock cognate protein interacts directly with the N-terminal region of the retinoblastoma gene product pRb. Identification of a novel region of pRb-mediating protein interaction. J Biol Chem. 1995;270:22571-6 pubmed
Lewis V, Hynes G, Zheng D, Saibil H, Willison K. T-complex polypeptide-1 is a subunit of a heteromeric particle in the eukaryotic cytosol. Nature. 1992;358:249-52 pubmed
Nadler S, Tepper M, Schacter B, Mazzucco C. Interaction of the immunosuppressant deoxyspergualin with a member of the Hsp70 family of heat shock proteins. Science. 1992;258:484-6 pubmed
image
image 1 :
Invitrogen MA3-006 image 1
Immunofluorescent analysis of Heat Shock Protein 70 using Heat Shock Protein 70 Monoclonal antibody (3A3) (Product# MA3-006) shows staining in HeLa cells. Heat Shock Protein 70 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 70 (Product# MA3-006) at a dilution of 1:100-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
image 2 :
Invitrogen MA3-006 image 2
Western blot analysis of Heat Shock Protein 70 (Hsp70) was performed by loading 50ug of the indicated whole cell lysates and 15ul of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a Hsp70 monoclonal antibody (Product # MA3-006) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
product information
Product Type :
Antibody
Product Name :
HSP70 Monoclonal Antibody (3A3)
Catalog # :
MA3-006
Quantity :
50 µg
Price :
US 425.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Amphibian, Arthropod, Avian, Canine, Drosophila, Fish, Human, Mollusca, Mouse, Non-human primate, Plant, Porcine, Rat, Yeast
Applications :
Gel Shift: Assay-dependent, Immunocytochemistry: 1:100, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: 2 µL, Western Blot: 1:1,000-1:5,000
Species :
Amphibian, Arthropod, Avian, Canine, Drosophila, Fish, Human, Mollusca, Mouse, Non-human primate, Plant, Porcine, Rat, Yeast
Clone :
3A3
Isotype :
IgG1
Storage :
-20° C, Avoid Freeze/Thaw Cycles
Description :
HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
Immunogen :
Human recombinant HSP70 overexpressed in E. coli.
Format :
Liquid
Applications w/Dilutions :
Gel Shift: Assay-dependent, Immunocytochemistry: 1:100, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: 2 µL, Western Blot: 1:1,000-1:5,000
Aliases :
68 kDa heat shock protein; 70 kda heat shock protein-2; 70 kilodalton heat shock protein; 87A; 87A locus; 87C locus; Bb; CG18743; CG31359; CG31359-PA; CG31366; CG31366-PA; CG31449; CG6489; chaperone; dHSP70; dhsp70Aa; Dmel CG31359; Dmel CG31366; Dmel_CG31359; Dmel_CG31366; Dm-hsp70; DmHSP70AA; DMHSP7D1; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; Hcp70.1; heat shock 10kDa protein 1-like; heat shock 70; heat shock 70 kD protein 1; heat shock 70 kD protein 2; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1-Hom; Heat shock 70 kDa protein 1L; heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; heat shock 70 kDa-like protein 1; heat shock 70kD protein 1A; heat shock 70kD protein 1B; heat shock 70kD protein 1B (mapped); heat shock 70kD protein-like 1; heat shock 70kDa protein 1A; heat shock 70kDa protein 1B; heat shock 70kDa protein 1-like; heat shock cognate 70-kd protein, tandem duplicate 2; Heat shock protein; heat shock protein 1A; heat shock protein 1B; heat shock protein 1-like; heat shock protein 70; heat shock protein 70.1; heat shock protein 70.2; heat shock protein 70-1; Heat shock protein 70-1l; Heat shock protein 70Aa; Heat shock protein 70Ab; Heat shock protein 70Ba; Heat shock protein 70Bb; Heat shock protein 70Bc; heat shock protein cognate 70, testis; heat shock protein family A (Hsp70) member 1 like; heat shock protein family A (Hsp70) member 1A; heat shock protein family A (Hsp70) member 1B; heat shock protein, 70 kDa 1; heat shock protein, 70 kDa 3; heat shock protein-70; heat shock-induced protein; heat shock-related protein hsc70t; heat-shock 70-kilodalton protein 1A; heat-shock 70-kilodalton protein 1B; heat-shock protein 70; Heat-shock-protein-70Aa; Heat-shock-protein-70Bb; HEL S 103; HEL-S-103; hs70; Hsc70t; HSP; Hsp 70; hsp68; Hsp70; Hsp-70; HSP70 1; HSP70 1B; HSP70 2; hsp70 87A; hsp70 87C; hsp70 Aa; HSP70 AceyltK77; hsp70(87A); Hsp70(87C); HSP70.1; HSP70.1/2; HSP70.1/HSP70.2; HSP70.2; Hsp70.3; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-1B; HSP70-1L; HSP70-2; Hsp70-3; HSP70-87A7; HSP70-87C1; hsp70a; hsp70A1; Hsp70A7d; Hsp70Aa; Hsp-70Aa; Hsp70Aa-PA; Hsp70Ab; Hsp70B; Hsp70Ba; Hsp70Bb; Hsp70Bbc; Hsp70Bb-PA; Hsp70Bc; HSP70-HOM; HSP70I; HSP70T; HSP72; Hspa1; Hspa1a; HSPA1B; Hspa1l; Hspa2; Hspa70; HSX70; hum70t; inducible heat shock protein 70; LP05203p; LP05233p; LP08776p; Major heat shock 70 kDa protein Aa; Major heat shock 70 kDa protein Ab; Major heat shock 70 kDa protein Ba; Major heat shock 70 kDa protein Bb; Major heat shock 70 kDa protein Bc; Msh5; protein 70; si:ch73-46h18.6; si:dkeyp-69e1.1; Spermatid-specific heat shock protein 70; zgc:174006
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Invitrogen
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