Endothelin 1 Monoclonal Antibody (TR.ET.48.5) | Invitrogen MA3-005 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Endothelin 1 Monoclonal Antibody (TR.ET.48.5)

catalog :

MA3-005

quantity :

100 µL

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

TR.ET.48.5

reactivity :

human, mouse, rat, pigs , domestic sheep

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 41

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:1000; fig 5g	Wang T, Liu C, Pan L, Liu Z, Li C, Lin J, et al. Inhibition of p38 MAPK Mitigates Lung Ischemia Reperfusion Injury by Reducing Blood-Air Barrier Hyperpermeability. Front Pharmacol. 2020;11:569251 pubmed publisher
immunohistochemistry - paraffin section; rat; 1:250; loading ...; fig 2i	Hirako S, Tsuda H, Kotani T, Sumigama S, Mano Y, Nakano T, et al. Antenatal Saireito (TJ-114) Can Improve Pulmonary Hypoplasia and Pulmonary Vascular Remodeling in Nitrofen-Induced Congenital Diaphragmatic Hernia. Phytother Res. 2016;30:1474-80 pubmed publisher
immunohistochemistry; mouse; 1:200; loading ...; fig 10	Chang F, Flavahan S, Flavahan N. Immature endothelial cells initiate endothelin-mediated constriction of newborn arteries. J Physiol. 2016;594:4933-44 pubmed publisher
immunohistochemistry; human; 1:250	Tamkus D, Sikorskii A, Gallo K, Wiese D, Leece C, Madhukar B, et al. Endothelin-1 enriched tumor phenotype predicts breast cancer recurrence. ISRN Oncol. 2013;2013:385398 pubmed publisher
immunohistochemistry - paraffin section; human; 1:250	Larson S, Zhang X, Dumpit R, Coleman I, Lakely B, Roudier M, et al. Characterization of osteoblastic and osteolytic proteins in prostate cancer bone metastases. Prostate. 2013;73:932-40 pubmed publisher
immunohistochemistry; rat	Cetinkaya Demir B, Uyar Y, Ozbilgin K, Kose C. Effect of raloxifene and atorvastatin in atherosclerotic process in ovariectomized rats. J Obstet Gynaecol Res. 2013;39:229-36 pubmed publisher
immunohistochemistry; pigs	Hessheimer A, Fondevila C, Taura P, Munoz J, Sanchez O, Fuster J, et al. Decompression of the portal bed and twice-baseline portal inflow are necessary for the functional recovery of a "small-for-size" graft. Ann Surg. 2011;253:1201-10 pubmed publisher
immunohistochemistry; human	Taghizadeh R, Noh M, Huh Y, Ciusani E, Sigalotti L, Maio M, et al. CXCR6, a newly defined biomarker of tissue-specific stem cell asymmetric self-renewal, identifies more aggressive human melanoma cancer stem cells. PLoS ONE. 2010;5:e15183 pubmed publisher
western blot; rat; 1:3000	Xu E, Kantores C, Ivanovska J, Engelberts D, Kavanagh B, McNamara P, et al. Rescue treatment with a Rho-kinase inhibitor normalizes right ventricular function and reverses remodeling in juvenile rats with chronic pulmonary hypertension. Am J Physiol Heart Circ Physiol. 2010;299:H1854-64 pubmed publisher
immunohistochemistry; human; 1:500	Lee H, Lim Y, Kim B, Kim M, Min H, Hwang J, et al. Clinicopathologic efficacy of copper bromide plus/yellow laser (578 nm with 511 nm) for treatment of melasma in Asian patients. Dermatol Surg. 2010;36:885-93 pubmed publisher
immunohistochemistry; human; 1:250	Sato Y, Sawada S, Kozaka K, Harada K, Sasaki M, Matsui O, et al. Significance of enhanced expression of nitric oxide syntheses in splenic sinus lining cells in altered portal hemodynamics of idiopathic portal hypertension. Dig Dis Sci. 2007;52:1987-94 pubmed
immunohistochemistry; human; 1:1000	Clausen H, Larsen L, Jørgensen A, Bzorek M. The human placenta from heavy smokers: evaluation of vasoactive peptides by immunohistochemistry. APMIS. 2007;115:22-9 pubmed
immunohistochemistry; human; 1:25	Tse G, Chaiwun B, Lau K, Scolyer R, Lee C, Karim R, et al. Endothelin-1 expression correlates with atypical histological features in mammary phyllodes tumours. J Clin Pathol. 2007;60:1051-6 pubmed
western blot; human	Spinella F, Rosanò L, Di Castro V, Decandia S, Albini A, Nicotra M, et al. Green tea polyphenol epigallocatechin-3-gallate inhibits the endothelin axis and downstream signaling pathways in ovarian carcinoma. Mol Cancer Ther. 2006;5:1483-92 pubmed
immunohistochemistry; human	Frank K, Zeier M, Gross M, Waldherr R, Ritz E, Amann K. Comprehensive immunohistological analysis of the endothelin system in human kidney grafts. Nephrol Dial Transplant. 2006;21:1365-72 pubmed
immunohistochemistry; human	Rosanò L, Spinella F, Di Castro V, Nicotra M, Dedhar S, De Herreros A, et al. Endothelin-1 promotes epithelial-to-mesenchymal transition in human ovarian cancer cells. Cancer Res. 2005;65:11649-57 pubmed
immunohistochemistry; human; 1:500	Wulfing C, Eltze E, Yamini J, Wülfing P, Bierer S, Bocker W, et al. Expression of the endothelin axis in bladder cancer: relationship to clinicopathologic parameters and long-term survival. Eur Urol. 2005;47:593-600 pubmed
immunohistochemistry; human; 1:200	Levy M, Maurey C, Chailley Heu B, Martinovic J, Jaubert F, Israel Biet D. Developmental changes in endothelial vasoactive and angiogenic growth factors in the human perinatal lung. Pediatr Res. 2005;57:248-53 pubmed
western blot; domestic sheep	Ross G, Oishi P, Azakie A, Fratz S, Fitzgerald R, Johengen M, et al. Endothelial alterations during inhaled NO in lambs with pulmonary hypertension: implications for rebound hypertension. Am J Physiol Lung Cell Mol Physiol. 2005;288:L27-35 pubmed
	Steven S, Oelze M, Hausding M, Roohani S, Kashani F, Kröller Schön S, et al. The Endothelin Receptor Antagonist Macitentan Improves Isosorbide-5-Mononitrate (ISMN) and Isosorbide Dinitrate (ISDN) Induced Endothelial Dysfunction, Oxidative Stress, and Vascular Inflammation. Oxid Med Cell Longev. 2018;2018:7845629 pubmed publisher
	Rogers N, Yao M, Sembrat J, George M, Knupp H, Ross M, et al. Cellular, pharmacological, and biophysical evaluation of explanted lungs from a patient with sickle cell disease and severe pulmonary arterial hypertension. Pulm Circ. 2013;3:936-51 pubmed publisher
	Xie G, Choi S, Syn W, Michelotti G, Swiderska M, Karaca G, et al. Hedgehog signalling regulates liver sinusoidal endothelial cell capillarisation. Gut. 2013;62:299-309 pubmed publisher
	Saunders R, Scheiner Bobis G. Ouabain stimulates endothelin release and expression in human endothelial cells without inhibiting the sodium pump. Eur J Biochem. 2004;271:1054-62 pubmed
	Fried G, Sand A, Ostlund E, Andersson E, Bystrom B, Ståbi B. Endothelin-1 and macrophage colony-stimulating factor are co-localized in human amnion membrane cells and secreted into amniotic fluid. Mol Hum Reprod. 2003;9:719-24 pubmed
	Black S, Mata Greenwood E, Dettman R, Ovadia B, Fitzgerald R, Reinhartz O, et al. Emergence of smooth muscle cell endothelin B-mediated vasoconstriction in lambs with experimental congenital heart disease and increased pulmonary blood flow. Circulation. 2003;108:1646-54 pubmed
	Kondo T, Vicent D, Suzuma K, Yanagisawa M, King G, Holzenberger M, et al. Knockout of insulin and IGF-1 receptors on vascular endothelial cells protects against retinal neovascularization. J Clin Invest. 2003;111:1835-42 pubmed
	Granchi S, Vannelli G, Vignozzi L, Crescioli C, Ferruzzi P, Mancina R, et al. Expression and regulation of endothelin-1 and its receptors in human penile smooth muscle cells. Mol Hum Reprod. 2002;8:1053-64 pubmed
	Shen J, Nakanishi T, Gu H, Miyagawa Tomita S, Wu G, Momma K, et al. The role of endothelin in oxygen-induced contraction of the ductus arteriosus in rabbit and rat fetuses. Heart Vessels. 2002;16:181-8 pubmed
	Porter K. Use of in vitro organ cultures of human saphenous vein as a model for intimal proliferation. Methods Mol Biol. 2002;206:199-214 pubmed
	Ovadia B, Bekker J, Fitzgerald R, Kon A, Thelitz S, Johengen M, et al. Nitric oxide-endothelin-1 interactions after acute ductal constriction in fetal lambs. Am J Physiol Heart Circ Physiol. 2002;282:H862-71 pubmed
	Lee J, Chun Y, Lee I, Tuder R, Hong S, Shim T, et al. Pathogenic role of endothelin 1 in hemodynamic dysfunction in experimental acute pulmonary thromboembolism. Am J Respir Crit Care Med. 2001;164:1282-7 pubmed
	Bagnato A, Rosano L, Di Castro V, Albini A, Salani D, Varmi M, et al. Endothelin receptor blockade inhibits proliferation of Kaposi's sarcoma cells. Am J Pathol. 2001;158:841-7 pubmed
	McMullan D, Bekker J, Johengen M, Hendricks Munoz K, Gerrets R, Black S, et al. Inhaled nitric oxide-induced rebound pulmonary hypertension: role for endothelin-1. Am J Physiol Heart Circ Physiol. 2001;280:H777-85 pubmed
	Salani D, Di Castro V, Nicotra M, Rosano L, Tecce R, Venuti A, et al. Role of endothelin-1 in neovascularization of ovarian carcinoma. Am J Pathol. 2000;157:1537-47 pubmed
	Aguirre J, Morrell N, Long L, Clift P, Upton P, Polak J, et al. Vascular remodeling and ET-1 expression in rat strains with different responses to chronic hypoxia. Am J Physiol Lung Cell Mol Physiol. 2000;278:L981-7 pubmed
	Bagnato A, Salani D, Di Castro V, Wu Wong J, Tecce R, Nicotra M, et al. Expression of endothelin 1 and endothelin A receptor in ovarian carcinoma: evidence for an autocrine role in tumor growth. Cancer Res. 1999;59:720-7 pubmed
	Peri A, Fantoni G, Granchi S, Vannelli G, Barni T, Amerini S, et al. Gene expression of endothelin-1, endothelin-converting enzyme-1, and endothelin receptors in human epididymis. J Clin Endocrinol Metab. 1997;82:3797-806 pubmed
	Mathieu M, Vittet D, Laliberte M, Laliberte F, Nonotte I, Hamroun D, et al. Endothelin expression in human megakaryoblastic leukemia cell lines and normal platelet precursors. Regul Pept. 1997;68:91-7 pubmed
	Nelson J, Chan Tack K, Hedican S, Magnuson S, Opgenorth T, Bova G, et al. Endothelin-1 production and decreased endothelin B receptor expression in advanced prostate cancer. Cancer Res. 1996;56:663-8 pubmed
	Saenz de Tejada I, Carson M, de las Morenas A, Goldstein I, Traish A. Endothelin: localization, synthesis, activity, and receptor types in human penile corpus cavernosum. Am J Physiol. 1991;261:H1078-85 pubmed
	Traish A, Moran E, Daley J, de las Morenas A, Saenz de Tejada I. Monoclonal antibodies to human endothelin-1: characterization and utilization in radioimmunoassay and immunocytochemistry. Hybridoma. 1992;11:147-63 pubmed

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product information

Product Type :

Antibody

Product Name :

Endothelin 1 Monoclonal Antibody (TR.ET.48.5)

Catalog # :

MA3-005

Quantity :

100 µL

Price :

US 399.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human, Mouse, Ovine, Rat

Applications :

Flow Cytometry: 0.5 µg/test, Immunocytochemistry: 1:200-1:1,000, Immunohistochemistry (Frozen): 1:250, Immunohistochemistry (Paraffin): 1:250, Radioimmune assays: 1:25,000, Western Blot: 1:100-1:1,000

Species :

Human, Mouse, Ovine, Rat

Clone :

TR.ET.48.5

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen :

Pure endothelin-1 conjugated to KLH.

Format :

Liquid

Applications w/Dilutions :

Aliases :

ARCND3; Big endothelin 1; Big endothelin-1; Big ET; Big ET 1; Big ET1; BigET; EDN1; endothelin 1; endothelin-1; Et; ET1; ET-1; HDLCQ7; PPET1; ppET-1; preproendothelin; preproendothelin-1; preproET; QME

more info or order :

Invitrogen product webpage