VCP Monoclonal Antibody (5) | Invitrogen MA3-004 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

VCP Monoclonal Antibody (5)

catalog :

MA3-004

quantity :

100 µg

price :

US 425.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

Saccharomycetales, human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 26

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig 4a	Matějů D, Franzmann T, Patel A, Kopach A, Boczek E, Maharana S, et al. An aberrant phase transition of stress granules triggered by misfolded protein and prevented by chaperone function. EMBO J. 2017;36:1669-1687 pubmed publisher
western blot; human; fig 2	Koizumi S, Irie T, Hirayama S, Sakurai Y, Yashiroda H, Naguro I, et al. The aspartyl protease DDI2 activates Nrf1 to compensate for proteasome dysfunction. elife. 2016;5: pubmed publisher
western blot; human; fig 1b	Wang T, Xu W, Qin M, Yang Y, Bao P, Shen F, et al. Pathogenic Mutations in the Valosin-containing Protein/p97(VCP) N-domain Inhibit the SUMOylation of VCP and Lead to Impaired Stress Response. J Biol Chem. 2016;291:14373-84 pubmed publisher
immunocytochemistry; mouse; 1:2000; fig s2 western blot; mouse; 1:5000; fig 1	Rodriguez Ortiz C, Flores J, Valenzuela J, Rodriguez G, Zumkehr J, Tran D, et al. The Myoblast C2C12 Transfected with Mutant Valosin-Containing Protein Exhibits Delayed Stress Granule Resolution on Oxidative Stress. Am J Pathol. 2016;186:1623-34 pubmed publisher
immunohistochemistry - paraffin section; human; 1:5000; loading ...; tbl 1	Ito M, Nakamura K, Mori F, Miki Y, Tanji K, Wakabayashi K. Novel eosinophilic neuronal cytoplasmic inclusions in the external cuneate nucleus of humans. Neuropathology. 2016;36:441-447 pubmed publisher
immunohistochemistry - paraffin section; human; 1:5,000	Mori F, Watanabe Y, Miki Y, Tanji K, Odagiri S, Eto K, et al. Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases. Neuropathology. 2014;34:140-7 pubmed
immunohistochemistry; mouse; fig 6	Kuijpers M, van Dis V, Haasdijk E, Harterink M, Vocking K, Post J, et al. Amyotrophic lateral sclerosis (ALS)-associated VAPB-P56S inclusions represent an ER quality control compartment. Acta Neuropathol Commun. 2013;1:24 pubmed publisher
western blot; Saccharomycetales; 1:3000	Buchan J, Kolaitis R, Taylor J, Parker R. Eukaryotic stress granules are cleared by autophagy and Cdc48/VCP function. Cell. 2013;153:1461-74 pubmed publisher
immunohistochemistry; human	Mori F, Tanji K, Toyoshima Y, Sasaki H, Yoshida M, Kakita A, et al. Valosin-containing protein immunoreactivity in tauopathies, synucleinopathies, polyglutamine diseases and intranuclear inclusion body disease. Neuropathology. 2013;33:637-44 pubmed publisher
immunoprecipitation; human immunohistochemistry; human; 1:2000	Rodriguez Ortiz C, Hoshino H, Cheng D, Liu Yescevitz L, Blurton Jones M, Wolozin B, et al. Neuronal-specific overexpression of a mutant valosin-containing protein associated with IBMPFD promotes aberrant ubiquitin and TDP-43 accumulation and cognitive dysfunction in transgenic mice. Am J Pathol. 2013;183:504-15 pubmed publisher
western blot; human; 1:3000	Kim N, Tresse E, Kolaitis R, Molliex A, Thomas R, Alami N, et al. VCP is essential for mitochondrial quality control by PINK1/Parkin and this function is impaired by VCP mutations. Neuron. 2013;78:65-80 pubmed publisher
immunohistochemistry; human	Hackman P, Sarparanta J, Lehtinen S, Vihola A, EvilÃ¤ A, Jonson P, et al. Welander distal myopathy is caused by a mutation in the RNA-binding protein TIA1. Ann Neurol. 2013;73:500-9 pubmed publisher
immunohistochemistry; rat; 1:500 western blot; rat; 1:10000	Cayli S, Ocakli S, Erdemir F, Tas U, Aslan H, Yener T, et al. Developmental expression of p97/VCP (Valosin-containing protein) and Jab1/CSN5 in the rat testis and epididymis. Reprod Biol Endocrinol. 2011;9:117 pubmed publisher
western blot; human	Iida Y, Fujimori T, Okawa K, Nagata K, Wada I, Hosokawa N. SEL1L protein critically determines the stability of the HRD1-SEL1L endoplasmic reticulum-associated degradation (ERAD) complex to optimize the degradation kinetics of ERAD substrates. J Biol Chem. 2011;286:16929-39 pubmed publisher
western blot; human	Ballar P, Pabuccuoglu A, Kose F. Different p97/VCP complexes function in retrotranslocation step of mammalian ER-associated degradation (ERAD). Int J Biochem Cell Biol. 2011;43:613-21 pubmed publisher
immunocytochemistry; human western blot; mouse	Tresse E, Salomons F, Vesa J, Bott L, Kimonis V, Yao T, et al. VCP/p97 is essential for maturation of ubiquitin-containing autophagosomes and this function is impaired by mutations that cause IBMPFD. Autophagy. 2010;6:217-27 pubmed
western blot; human	Ballar P, Ors A, Yang H, Fang S. Differential regulation of CFTRDeltaF508 degradation by ubiquitin ligases gp78 and Hrd1. Int J Biochem Cell Biol. 2010;42:167-73 pubmed publisher
immunohistochemistry; human; 1:50000	Greenberg S, Watts G, Kimonis V, Amato A, Pinkus J. Nuclear localization of valosin-containing protein in normal muscle and muscle affected by inclusion-body myositis. Muscle Nerve. 2007;36:447-54 pubmed
ELISA; human	Saffar A, Alphonse M, Shan L, Hayglass K, Simons F, Gounni A. IgE modulates neutrophil survival in asthma: role of mitochondrial pathway. J Immunol. 2007;178:2535-41 pubmed
	Wu X, Spence J, Das T, Yuan X, Chen C, Zhang Y, et al. Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase. Cell Chem Biol. 2020;27:571-585.e6 pubmed publisher
	Wang B, Maxwell B, Joo J, Gwon Y, Messing J, Mishra A, et al. ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97. Mol Cell. 2019;74:742-757.e8 pubmed publisher
	Palomo G, Granatiero V, Kawamata H, Konràd C, Kim M, Arreguin A, et al. Parkin is a disease modifier in the mutant SOD1 mouse model of ALS. EMBO Mol Med. 2018;10: pubmed publisher
	Bento A, Bippes C, Kohler C, Hemion C, Frank S, Neutzner A. UBXD1 is a mitochondrial recruitment factor for p97/VCP and promotes mitophagy. Sci Rep. 2018;8:12415 pubmed publisher
	Ash P, Stanford E, Al Abdulatif A, Ramirez Cardenas A, Ballance H, Boudeau S, et al. Dioxins and related environmental contaminants increase TDP-43 levels. Mol Neurodegener. 2017;12:35 pubmed publisher
	Auranen M, Palmio J, Ylikallio E, Huovinen S, Paetau A, Sandell S, et al. PFKM gene defect and glycogen storage disease GSDVII with misleading enzyme histochemistry. Neurol Genet. 2015;1:e7 pubmed publisher
	Vij N, Fang S, Zeitlin P. Selective inhibition of endoplasmic reticulum-associated degradation rescues DeltaF508-cystic fibrosis transmembrane regulator and suppresses interleukin-8 levels: therapeutic implications. J Biol Chem. 2006;281:17369-78 pubmed

image

image 1 :

Immunofluorescent analysis of VCP using VCP Monoclonal antibody (5) (Product# MA3-004) shows staining in HeLa cells. VCP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing VCP (Product# MA3-004) at a dilution of 1:20-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

VCP Monoclonal Antibody (5)

Catalog # :

MA3-004

Quantity :

100 µg

Price :

US 425.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Flow Cytometry: 1/400, Immunocytochemistry: 1:20-1:200, Immunohistochemistry (Frozen): 1:500, Immunohistochemistry (Paraffin): 1:500, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Species :

Human, Mouse, Rat

Clone :

Isotype :

IgG2a

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.

Immunogen :

Synthetic peptide corresponding to residues C G(792) G S V Y T E D N D D D L Y G(806) of mouse VCP.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1/400, Immunocytochemistry: 1:20-1:200, Immunohistochemistry (Frozen): 1:500, Immunohistochemistry (Paraffin): 1:500, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Aliases :

15S Mg(2+)-ATPase p97 subunit; 3110001E05; AAA ATPase p97; ALS14; CDC48; cell division cycle gene CDC48; CMT2Y; epididymis luminal protein 220; epididymis secretory protein Li 70; HEL-220; HEL-S-70; homolog of yeast cdc48; IBMPFD; IBMPFD1; Inv protein; or HEL-S-70; p97; p97 atpase; p97/VCP; Protein CDC48; si:ch211-113n10.2; TER ATPase; TERA; transitional endoplasmic reticulum ATPase; valosin containing protein; valosin-containing protein; Vcp; wu:fd16d05; wu:fj63d11; yeast Cdc48p homolog

more info or order :

Invitrogen product webpage