CD11a Monoclonal Antibody (TS1/22) | Invitrogen MA11A10 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD11a Monoclonal Antibody (TS1/22)

catalog :

MA11A10

quantity :

200 µg

price :

US 481.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

TS1/22

reactivity :

human, mouse

application :

western blot, ELISA, flow cytometry, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 47

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig s2b	Liu H, Dai X, Cao X, Yan H, Ji X, Zhang H, et al. PRDM4 mediates YAP-induced cell invasion by activating leukocyte-specific integrin β2 expression. EMBO Rep. 2018;19: pubmed publisher
blocking or activating experiments; human; loading ...; fig 5c	Yeap W, Wong K, Shimasaki N, Teo E, Quek J, Yong H, et al. CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes. Sci Rep. 2016;6:34310 pubmed publisher
blocking or activating experiments; human; 10 ug/ml; loading ...; fig 3b	Kawamoto E, Okamoto T, Takagi Y, Honda G, Suzuki K, Imai H, et al. LFA-1 and Mac-1 integrins bind to the serine/threonine-rich domain of thrombomodulin. Biochem Biophys Res Commun. 2016;473:1005-1012 pubmed publisher
flow cytometry; human; fig 2	Tuli A, Thiery J, James A, Michelet X, Sharma M, Garg S, et al. Arf-like GTPase Arl8b regulates lytic granule polarization and natural killer cell-mediated cytotoxicity. Mol Biol Cell. 2013;24:3721-35 pubmed publisher
blocking or activating experiments; human	Podgrabinska S, Kamalu O, Mayer L, Shimaoka M, Snoeck H, Randolph G, et al. Inflamed lymphatic endothelium suppresses dendritic cell maturation and function via Mac-1/ICAM-1-dependent mechanism. J Immunol. 2009;183:1767-79 pubmed publisher
flow cytometry; human	Kanno H, Watabe D, Shimizu N, Sawai T. Adhesion of Epstein-Barr virus-positive natural killer cell lines to cultured endothelial cells stimulated with inflammatory cytokines. Clin Exp Immunol. 2008;151:519-27 pubmed publisher
blocking or activating experiments; human; 50 ug/ml	Kofler S, Schlichting C, Jankl S, Nickel T, Weis M. Dual mode of HMG-CoA reductase inhibition on dendritic cell invasion. Atherosclerosis. 2008;197:105-10 pubmed
blocking or activating experiments; human	Watabe D, Kanno H, Yoshida A, Kurose A, Akasaka T, Sawai T. Adhesion of peripheral blood mononuclear cells and CD4+ T cells from patients with psoriasis to cultured endothelial cells via the interaction between lymphocyte function-associated antigen type 1 and intercellular adhesion molecule 1. Br J Dermatol. 2007;157:259-65 pubmed
blocking or activating experiments; human	Simonson W, Franco S, Huttenlocher A. Talin1 regulates TCR-mediated LFA-1 function. J Immunol. 2006;177:7707-14 pubmed
ELISA; human	Combs J, Kim S, Tan S, Ligon L, Holzbaur E, Kuhn J, et al. Recruitment of dynein to the Jurkat immunological synapse. Proc Natl Acad Sci U S A. 2006;103:14883-8 pubmed
blocking or activating experiments; human	Sonnet C, Lafuste P, Arnold L, Brigitte M, Poron F, Authier F, et al. Human macrophages rescue myoblasts and myotubes from apoptosis through a set of adhesion molecular systems. J Cell Sci. 2006;119:2497-507 pubmed
blocking or activating experiments; human; 20 ug/ml	Konstandin M, Sester U, Klemke M, Weschenfelder T, Wabnitz G, Samstag Y. A novel flow-cytometry-based assay for quantification of affinity and avidity changes of integrins. J Immunol Methods. 2006;310:67-77 pubmed
blocking or activating experiments; human; 50 ug/ml	Schlichting C, Schareck W, Weis M. Dendritic cell adhesion is enhanced on endothelial cells preexposed to calcineurin inhibitors. J Cardiovasc Pharmacol. 2005;46:250-4 pubmed
flow cytometry; human	Agea E, Russano A, Bistoni O, Mannucci R, Nicoletti I, Corazzi L, et al. Human CD1-restricted T cell recognition of lipids from pollens. J Exp Med. 2005;202:295-308 pubmed
flow cytometry; human	Peterson M, Jin R, Hyduk S, Duchesneau P, Cybulsky M, Waddell T. Monocyte adhesion to xenogeneic endothelium during laminar flow is dependent on alpha-Gal-mediated monocyte activation. J Immunol. 2005;174:8072-81 pubmed
	Zhou F, Zhang F, Zarnitsyna V, Doudy L, Yuan Z, Li K, et al. The kinetics of E-selectin- and P-selectin-induced intermediate activation of integrin αLβ2 on neutrophils. J Cell Sci. 2021;134: pubmed publisher
	Cassioli C, Balint S, Compeer E, Felce J, Gamberucci A, Della Bella C, et al. Increasing LFA-1 Expression Enhances Immune Synapse Architecture and T Cell Receptor Signaling in Jurkat E6.1 Cells. Front Cell Dev Biol. 2021;9:673446 pubmed publisher
	Mancuso R, Casper J, Schmidt A, Krahenbuhl S, Weitz Schmidt G. Anti-αLβ2 antibodies reveal novel endocytotic cross-modulatory functionality. Br J Pharmacol. 2020;177:2696-2711 pubmed publisher
	Jung O, Beauvais D, Adams K, Rapraeger A. VLA-4 phosphorylation during tumor and immune cell migration relies on its coupling to VEGFR2 and CXCR4 by syndecan-1. J Cell Sci. 2019;132: pubmed publisher
	Kronstad L, Seiler C, Vergara R, Holmes S, Blish C. Differential Induction of IFN-? and Modulation of CD112 and CD54 Expression Govern the Magnitude of NK Cell IFN-? Response to Influenza A Viruses. J Immunol. 2018;201:2117-2131 pubmed publisher
	Guo H, Cranert S, Lu Y, Zhong M, Zhang S, Chen J, et al. Deletion of Slam locus in mice reveals inhibitory role of SLAM family in NK cell responses regulated by cytokines and LFA-1. J Exp Med. 2016;213:2187-207 pubmed publisher
	Lee J, Buzney C, Poznansky M, Sackstein R. Dynamic alterations in chemokine gradients induce transendothelial shuttling of human T cells under physiologic shear conditions. J Leukoc Biol. 2009;86:1285-94 pubmed publisher
	Chavakis E, Hain A, Vinci M, Carmona G, Bianchi M, Vajkoczy P, et al. High-mobility group box 1 activates integrin-dependent homing of endothelial progenitor cells. Circ Res. 2007;100:204-12 pubmed
	Iikura M, Ebisawa M, Yamaguchi M, Tachimoto H, Ohta K, Yamamoto K, et al. Transendothelial migration of human basophils. J Immunol. 2004;173:5189-95 pubmed
	Zen K, Utech M, Liu Y, Soto I, Nusrat A, Parkos C. Association of BAP31 with CD11b/CD18. Potential role in intracellular trafficking of CD11b/CD18 in neutrophils. J Biol Chem. 2004;279:44924-30 pubmed
	Dagia N, Harii N, Meli A, Sun X, Lewis C, Kohn L, et al. Phenyl methimazole inhibits TNF-alpha-induced VCAM-1 expression in an IFN regulatory factor-1-dependent manner and reduces monocytic cell adhesion to endothelial cells. J Immunol. 2004;173:2041-9 pubmed
	Ehlers R, Ustinov V, Chen Z, Zhang X, Rao R, Luscinskas F, et al. Targeting platelet-leukocyte interactions: identification of the integrin Mac-1 binding site for the platelet counter receptor glycoprotein Ibalpha. J Exp Med. 2003;198:1077-88 pubmed
	Riteau B, Barber D, Long E. Vav1 phosphorylation is induced by beta2 integrin engagement on natural killer cells upstream of actin cytoskeleton and lipid raft reorganization. J Exp Med. 2003;198:469-74 pubmed
	Horvath Arcidiacono J, Tsuyuki S, Mostowski H, Bloom E. Human natural killer cell activity against porcine targets: modulation by control of the oxidation-reduction environment and role of adhesion molecule interactions. Cell Immunol. 2003;222:35-44 pubmed
	Weis M, Schlichting C, Engleman E, Cooke J. Endothelial determinants of dendritic cell adhesion and migration: new implications for vascular diseases. Arterioscler Thromb Vasc Biol. 2002;22:1817-23 pubmed
	Tibaldi E, Salgia R, Reinherz E. CD2 molecules redistribute to the uropod during T cell scanning: implications for cellular activation and immune surveillance. Proc Natl Acad Sci U S A. 2002;99:7582-7 pubmed
	Morgan M, Labno C, van Seventer G, Denny M, Straus D, Burkhardt J. Superantigen-induced T cell:B cell conjugation is mediated by LFA-1 and requires signaling through Lck, but not ZAP-70. J Immunol. 2001;167:5708-18 pubmed
	Wang P, Malkovsky M. Different roles of the CD2 and LFA-1 T-cell co-receptors for regulating cytotoxic, proliferative, and cytokine responses of human V gamma 9/V delta 2 T cells. Mol Med. 2000;6:196-207 pubmed
	Crutchfield K, Shinde Patil V, Campbell C, Parkos C, Allport J, Goetz D. CD11b/CD18-coated microspheres attach to E-selectin under flow. J Leukoc Biol. 2000;67:196-205 pubmed
	Babina M, Mammeri K, Henz B. ICAM-3 (CD50) is expressed by human mast cells: induction of homotypic mast cell aggregation via ICAM-3. Cell Adhes Commun. 1999;7:195-209 pubmed
	Chan J, Hyduk S, Cybulsky M. Alpha 4 beta 1 integrin/VCAM-1 interaction activates alpha L beta 2 integrin-mediated adhesion to ICAM-1 in human T cells. J Immunol. 2000;164:746-53 pubmed
	Edwards C, Fisher K, Presta L, Bodary S. Mapping the intercellular adhesion molecule-1 and -2 binding site on the inserted domain of leukocyte function-associated antigen-1. J Biol Chem. 1998;273:28937-44 pubmed
	Petruzzelli L, Maduzia L, Springer T. Differential requirements for LFA-1 binding to ICAM-1 and LFA-1-mediated cell aggregation. J Immunol. 1998;160:4208-16 pubmed
	Wang W, Goldman L, Schleider D, Appenheimer M, Subjeck J, Repasky E, et al. Fever-range hyperthermia enhances L-selectin-dependent adhesion of lymphocytes to vascular endothelium. J Immunol. 1998;160:961-9 pubmed
	Poccia F, Boullier S, Lecoeur H, Cochet M, Poquet Y, Colizzi V, et al. Peripheral V gamma 9/V delta 2 T cell deletion and anergy to nonpeptidic mycobacterial antigens in asymptomatic HIV-1-infected persons. J Immunol. 1996;157:449-61 pubmed
	Stark J, Godding V, Sedgwick J, Busse W. Respiratory syncytial virus infection enhances neutrophil and eosinophil adhesion to cultured respiratory epithelial cells. Roles of CD18 and intercellular adhesion molecule-1. J Immunol. 1996;156:4774-82 pubmed
	Mobley J, Ennis E, Shimizu Y. Isolation and characterization of cell lines with genetically distinct mutations downstream of protein kinase C that result in defective activation-dependent regulation of T cell integrin function. J Immunol. 1996;156:948-56 pubmed
	Xie J, Li R, Kotovuori P, Vermot Desroches C, Wijdenes J, Arnaout M, et al. Intercellular adhesion molecule-2 (CD102) binds to the leukocyte integrin CD11b/CD18 through the A domain. J Immunol. 1995;155:3619-28 pubmed
	Sanchez Madrid F, Nagy J, Robbins E, Simon P, Springer T. A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule. J Exp Med. 1983;158:1785-1803 pubmed
	Kishimoto T, Hollander N, Roberts T, Anderson D, Springer T. Heterogeneous mutations in the beta subunit common to the LFA-1, Mac-1, and p150,95 glycoproteins cause leukocyte adhesion deficiency. Cell. 1987;50:193-202 pubmed
	Kishimoto T, O CONNOR K, Lee A, Roberts T, Springer T. Cloning of the beta subunit of the leukocyte adhesion proteins: homology to an extracellular matrix receptor defines a novel supergene family. Cell. 1987;48:681-90 pubmed
	Hale L, Martin M, McCollum D, Nunley J, Springer T, Singer K, et al. Immunohistologic analysis of the distribution of cell adhesion molecules within the inflammatory synovial microenvironment. Arthritis Rheum. 1989;32:22-30 pubmed

product information

Product Type :

Antibody

Product Name :

CD11a Monoclonal Antibody (TS1/22)

Catalog # :

MA11A10

Quantity :

200 µg

Price :

US 481.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

Flow Cytometry: 0.5 µg/test

Species :

Human, Mouse

Clone :

TS1/22

Isotype :

IgG1

Storage :

-20°C

Description :

CD11a (LFA-1 alpha, ITGAL) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. CD11a is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of CD11a to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. CD11a is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of CD11a function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti- CD11a antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. Two transcript variants encoding different isoforms of CD11a have been found. Diseases associated with CD11a dysfunction include benign cephalic histiocytosis and leukocyte adhesion deficiency.

Immunogen :

Human 180 kD alpha chain of the CD11alpha complex.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5 µg/test

Aliases :

(p180); alpha L integrin; antigen CD11A (p180); antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; antigen CD11A p180; CD11 antigen-like family member A; CD11a; integrin alpha L; integrin alphaL; integrin alpha-L; integrin alpha-L precursor; integrin gene promoter; integrin subunit alpha L; integrin surface receptor; integrin, alpha L; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); ITGAL; Leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte function-associated molecule 1 alpha chain; LFA-1; LFA-1 alpha; LFA1A; LFA-1A; Ly-15; Ly-21; lymphocyte antigen 15; lymphocyte function associated antigen 1, alpha polypeptide; lymphocyte function-associated antigen 1; lymphocyte function-associated antigen 1, alpha polypeptide; lymphocyte function-associated antigen-1

more info or order :

Invitrogen product webpage