S-peptide Epitope Tag Monoclonal Antibody (6.2) | Invitrogen MA1-981 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

S-peptide Epitope Tag Monoclonal Antibody (6.2)

catalog :

MA1-981

quantity :

100 µg

price :

US 325.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6.2

reactivity :

human, roundworm

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 5c	Fenech E, Lari F, Charles P, Fischer R, Laétitia Thézénas M, Bagola K, et al. Interaction mapping of endoplasmic reticulum ubiquitin ligases identifies modulators of innate immune signalling. elife. 2020;9: pubmed publisher
immunohistochemistry; roundworm	Schouteden C, Serwas D, Pálfy M, Dammermann A. The ciliary transition zone functions in cell adhesion but is dispensable for axoneme assembly in C. elegans. J Cell Biol. 2015;210:35-44 pubmed publisher
	Davidge B, Rebola K, Agbor L, Sigmund C, Singer J. Cul3 regulates cyclin E1 protein abundance via a degron located within the N-terminal region of cyclin E. J Cell Sci. 2019;132: pubmed publisher
	Schulz J, Avci D, Queisser M, Gutschmidt A, Dreher L, Fenech E, et al. Conserved cytoplasmic domains promote Hrd1 ubiquitin ligase complex formation for ER-associated degradation (ERAD). J Cell Sci. 2017;130:3322-3335 pubmed publisher
	Dong Q, Yin F, Gao F, Shen Y, Zhang F, Li Y, et al. Ccp1 Homodimer Mediates Chromatin Integrity by Antagonizing CENP-A Loading. Mol Cell. 2016;64:79-91 pubmed publisher
	Li F, Martienssen R, Cande W. Coordination of DNA replication and histone modification by the Rik1-Dos2 complex. Nature. 2011;475:244-8 pubmed publisher
	Hackbarth J, Lee S, Meng X, Vroman B, Kaufmann S, Karnitz L. S-peptide epitope tagging for protein purification, expression monitoring, and localization in mammalian cells. Biotechniques. 2004;37:835-9 pubmed

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product information

Product Type :

Antibody

Product Name :

S-peptide Epitope Tag Monoclonal Antibody (6.2)

Catalog # :

MA1-981

Quantity :

100 µg

Price :

US 325.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Tag

Applications :

Immunocytochemistry: 10 µg/mL, Immunohistochemistry: 10 µg/mL, Western Blot: 1 µg/mL

Species :

Tag

Clone :

6.2

Isotype :

IgG2b

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The S-Peptide epitope tag (S-Tag (TM)) can be used for numerous applications which include the analysis of localization, expression detection, and purification of polypeptides from mammalian cells. The small size of the tag (15 aa), the ability to purify tagged polypeptides without antibodies, and the very low expression of endogenous tissues in mammalian cells that react with S-Peptide make the S-Peptide antibody an attractive alternative to many existing epitope tags. Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene.

Immunogen :

Synthetic Peptide: K(1) E T A A A K F E R Q H M D S(15) C

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 10 µg/mL, Immunohistochemistry: 10 µg/mL, Western Blot: 1 µg/mL

Aliases :

S-tag

more info or order :

Invitrogen product webpage