Calcium Sensing Receptor Monoclonal Antibody (5C10, ADD) | Invitrogen MA1-934 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Calcium Sensing Receptor Monoclonal Antibody (5C10, ADD)

catalog :

MA1-934

quantity :

100 µg

price :

US 412.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5C10, ADD

reactivity :

human, mouse, rat, bovine, pigs

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 49

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; fig 4b	Kawakami K, Takeshita A, Furushima K, Miyajima M, Hatamura I, Kuro O M, et al. Persistent fibroblast growth factor 23 signalling in the parathyroid glands for secondary hyperparathyroidism in mice with chronic kidney disease. Sci Rep. 2017;7:40534 pubmed publisher
western blot; mouse; 1:500; loading ...; fig s3c	Corre T, Olinger E, Harris S, Traglia M, Ulivi S, Lenarduzzi S, et al. Common variants in CLDN14 are associated with differential excretion of magnesium over calcium in urine. Pflugers Arch. 2017;469:91-103 pubmed publisher
immunohistochemistry - paraffin section; human; fig 2a immunocytochemistry; human; loading ...; fig 2g	Balenga N, Azimzadeh P, Hogue J, Staats P, Shi Y, Koh J, et al. Orphan Adhesion GPCR GPR64/ADGRG2 Is Overexpressed in Parathyroid Tumors and Attenuates Calcium-Sensing Receptor-Mediated Signaling. J Bone Miner Res. 2017;32:654-666 pubmed publisher
western blot; rat; 1:200; loading ...; fig 2e	Guo Q, Xu H, Yang X, Zhao D, Liu S, Sun X, et al. Notch activation of Ca2+-sensing receptor mediates hypoxia-induced pulmonary hypertension. Hypertens Res. 2017;40:117-129 pubmed publisher
immunohistochemistry; mouse; 1:500 immunohistochemistry; rat; 1:500; fig 1 immunohistochemistry; human; 1:500; fig 1	Graca J, Schepelmann M, Brennan S, Reens J, Chang W, Yan P, et al. Comparative expression of the extracellular calcium-sensing receptor in the mouse, rat, and human kidney. Am J Physiol Renal Physiol. 2016;310:F518-33 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:500	Yasuoka Y, Sato Y, Healy J, Nonoguchi H, Kawahara K. pH-sensitive expression of calcium-sensing receptor (CaSR) in type-B intercalated cells of the cortical collecting ducts (CCD) in mouse kidney. Clin Exp Nephrol. 2015;19:771-82 pubmed publisher
immunohistochemistry; mouse; 1:100 western blot; mouse western blot; human	Xie R, Dong X, Wong C, Vallon V, Tang B, Sun J, et al. Molecular mechanisms of calcium-sensing receptor-mediated calcium signaling in the modulation of epithelial ion transport and bicarbonate secretion. J Biol Chem. 2014;289:34642-53 pubmed publisher
western blot; mouse; 1:500	Laporta J, Keil K, Vezina C, Hernandez L. Peripheral serotonin regulates maternal calcium trafficking in mammary epithelial cells during lactation in mice. PLoS ONE. 2014;9:e110190 pubmed publisher
immunocytochemistry; mouse; 1:100	Li H, Feinle Bisset C, Frisby C, Kentish S, Wittert G, Page A. Gastric neuropeptide W is regulated by meal-related nutrients. Peptides. 2014;62:6-14 pubmed publisher
immunocytochemistry; rat; 1:100	Fabbri S, Ciuffi S, Nardone V, Gomes A, Mavilia C, Zonefrati R, et al. PTH-C1: a rat continuous cell line expressing the parathyroid phenotype. Endocrine. 2014;47:90-9 pubmed publisher
flow cytometry; human	Paccou J, Boudot C, Mary A, Kamel S, Drüeke T, Fardellone P, et al. Determination and modulation of total and surface calcium-sensing receptor expression in monocytes in vivo and in vitro. PLoS ONE. 2013;8:e74800 pubmed publisher
western blot; mouse; 1:200 western blot; rat; 1:200	Guo Q, Huang J, Yamamura A, Yamamura H, Zimnicka A, Fernandez R, et al. Inhibition of the Ca(2+)-sensing receptor rescues pulmonary hypertension in rats and mice. Hypertens Res. 2014;37:116-24 pubmed publisher
immunohistochemistry - frozen section; rat; 1:500	Gu Q, Vysotskaya Z, Moss C, Kagira M, Gilbert C. Calcium-sensing receptor in rat vagal bronchopulmonary sensory neurons regulates the function of the capsaicin receptor TRPV1. Exp Physiol. 2013;98:1631-42 pubmed publisher
western blot; rat; 1:700 or 1:1000 immunoprecipitation; human	Loupy A, Ramakrishnan S, Wootla B, Chambrey R, de la Faille R, Bourgeois S, et al. PTH-independent regulation of blood calcium concentration by the calcium-sensing receptor. J Clin Invest. 2012;122:3355-67 pubmed publisher
western blot; human; 1:200 immunohistochemistry; rat; 1:100	Yamamura A, Guo Q, Yamamura H, Zimnicka A, Pohl N, Smith K, et al. Enhanced Ca(2+)-sensing receptor function in idiopathic pulmonary arterial hypertension. Circ Res. 2012;111:469-81 pubmed publisher
immunohistochemistry; rat; 1:100	Cheng S. Calcium-sensing receptor inhibits secretagogue-induced electrolyte secretion by intestine via the enteric nervous system. Am J Physiol Gastrointest Liver Physiol. 2012;303:G60-70 pubmed publisher
immunohistochemistry; human; 1:100-1:150	Haid D, Jordan Biegger C, Widmayer P, Breer H. Receptors responsive to protein breakdown products in g-cells and d-cells of mouse, swine and human. Front Physiol. 2012;3:65 pubmed publisher
immunocytochemistry; human; 1:100 western blot; human; 1:2000	Bonomini M, Giardinelli A, Morabito C, Di Silvestre S, Di Cesare M, Di Pietro N, et al. Calcimimetic R-568 and its enantiomer S-568 increase nitric oxide release in human endothelial cells. PLoS ONE. 2012;7:e30682 pubmed publisher
immunoprecipitation; human immunocytochemistry; human	Arulpragasam A, Magno A, Ingley E, Brown S, Conigrave A, Ratajczak T, et al. The adaptor protein 14-3-3 binds to the calcium-sensing receptor and attenuates receptor-mediated Rho kinase signalling. Biochem J. 2012;441:995-1006 pubmed publisher
immunocytochemistry; mouse	Tharmalingam S, Daulat A, Antflick J, Ahmed S, Nemeth E, Angers S, et al. Calcium-sensing receptor modulates cell adhesion and migration via integrins. J Biol Chem. 2011;286:40922-33 pubmed publisher
immunohistochemistry; human	Haid D, Widmayer P, Breer H. Nutrient sensing receptors in gastric endocrine cells. J Mol Histol. 2011;42:355-64 pubmed publisher
immunocytochemistry; human; 1:100	Chowdhury P, Pore D, Mahata N, Karmakar P, Pal A, Chakrabarti M. Thermostable direct hemolysin downregulates human colon carcinoma cell proliferation with the involvement of E-cadherin, and ?-catenin/Tcf-4 signaling. PLoS ONE. 2011;6:e20098 pubmed publisher
western blot; mouse	Koh J, Dar M, Untch B, Dixit D, Shi Y, Yang Z, et al. Regulator of G protein signaling 5 is highly expressed in parathyroid tumors and inhibits signaling by the calcium-sensing receptor. Mol Endocrinol. 2011;25:867-76 pubmed publisher
immunohistochemistry; mouse; 1:50	Liou A, Sei Y, Zhao X, Feng J, Lu X, Thomas C, et al. The extracellular calcium-sensing receptor is required for cholecystokinin secretion in response to L-phenylalanine in acutely isolated intestinal I cells. Am J Physiol Gastrointest Liver Physiol. 2011;300:G538-46 pubmed publisher
western blot; human	Lazarus S, Pretorius C, Khafagi F, Campion K, Brennan S, Conigrave A, et al. A novel mutation of the primary protein kinase C phosphorylation site in the calcium-sensing receptor causes autosomal dominant hypocalcemia. Eur J Endocrinol. 2011;164:429-35 pubmed publisher
western blot; human	Zhuang X, Adipietro K, Datta S, Northup J, Ray K. Rab1 small GTP-binding protein regulates cell surface trafficking of the human calcium-sensing receptor. Endocrinology. 2010;151:5114-23 pubmed publisher
western blot; human	Terranegra A, Ferraretto A, Dogliotti E, Scarpellini M, Corbetta S, Barbieri A, et al. Calcimimetic R-568 effects on activity of R990G polymorphism of calcium-sensing receptor. J Mol Endocrinol. 2010;45:245-56 pubmed publisher
immunohistochemistry; rat	Shiizaki K, Hatamura I, Fukagawa M, Nakazawa E, Saji F, Watanabe Y, et al. Development of a technique for introduction of an expressed complementary deoxyribonucleic acid into parathyroid cells by direct injection. Endocrinology. 2010;151:4031-8 pubmed publisher
immunocytochemistry; human; 1:1000 immunohistochemistry; human; 1:1000 western blot; human; 1:1000	Milara J, Mata M, Serrano A, Peiró T, Morcillo E, Cortijo J. Extracellular calcium-sensing receptor mediates human bronchial epithelial wound repair. Biochem Pharmacol. 2010;80:236-46 pubmed publisher
western blot; human	McCormick W, Atkinson Dell R, Campion K, Mun H, Conigrave A, Ward D. Increased receptor stimulation elicits differential calcium-sensing receptor(T888) dephosphorylation. J Biol Chem. 2010;285:14170-7 pubmed publisher
immunohistochemistry; mouse	Caudrillier A, Mentaverri R, Brazier M, Kamel S, Massy Z. Calcium-sensing receptor as a potential modulator of vascular calcification in chronic kidney disease. J Nephrol. 2010;23:17-22 pubmed
immunocytochemistry; human; 1:1000 western blot; human; 1:1000	Cortijo J, Milara J, Mata M, Donet E, Gavara N, Peel S, et al. Nickel induces intracellular calcium mobilization and pathophysiological responses in human cultured airway epithelial cells. Chem Biol Interact. 2010;183:25-33 pubmed publisher
immunocytochemistry; human immunohistochemistry; human western blot; human immunohistochemistry; bovine western blot; bovine	Alam M, Kirton J, Wilkinson F, Towers E, Sinha S, Rouhi M, et al. Calcification is associated with loss of functional calcium-sensing receptor in vascular smooth muscle cells. Cardiovasc Res. 2009;81:260-8 pubmed publisher
immunocytochemistry; rat; 1:50 immunohistochemistry; rat; 1:50 western blot; rat	Heyeraas K, Haug S, Bukoski R, Awumey E. Identification of a Ca2+-sensing receptor in rat trigeminal ganglia, sensory axons, and tooth dental pulp. Calcif Tissue Int. 2008;82:57-65 pubmed publisher
western blot; rat	Awumey E, Howlett A, Putney J, Diz D, Bukoski R. Ca(2+) mobilization through dorsal root ganglion Ca(2+)-sensing receptor stably expressed in HEK293 cells. Am J Physiol Cell Physiol. 2007;292:C1895-905 pubmed
western blot; human	Ray K, Adipietro K, Chen C, Northup J. Elucidation of the role of peptide linker in calcium-sensing receptor activation process. J Biol Chem. 2007;282:5310-7 pubmed
western blot; pigs ; 0.2 ug/ml immunohistochemistry; rat; 10 ug/ml western blot; rat; 0.2 ug/ml	Weston A, Absi M, Ward D, Ohanian J, Dodd R, Dauban P, et al. Evidence in favor of a calcium-sensing receptor in arterial endothelial cells: studies with calindol and Calhex 231. Circ Res. 2005;97:391-8 pubmed
	Fabbri S, Zonefrati R, Galli G, Gronchi G, Perigli G, Borrelli A, et al. In Vitro Control of Genes Critical for Parathyroid Embryogenesis by Extracellular Calcium. J Endocr Soc. 2020;4:bvaa058 pubmed publisher
	Centeno P, HERBERGER A, Mun H, Tu C, Nemeth E, Chang W, et al. Phosphate acts directly on the calcium-sensing receptor to stimulate parathyroid hormone secretion. Nat Commun. 2019;10:4693 pubmed publisher
	Feng J, Kang C, Wang C, Ding L, Zhu W, Hang S. L-phenylalanine Increased Gut Hormone Secretion through Calcium-Sensing Receptor in the Porcine Duodenum. Animals (Basel). 2019;9: pubmed publisher
	Makita N, Ando T, Sato J, Manaka K, Mitani K, Kikuchi Y, et al. Cinacalcet corrects biased allosteric modulation of CaSR by AHH autoantibody. JCI Insight. 2019;4: pubmed publisher
	Weaver S, Fricke H, Xie C, Lipinski R, Vezina C, Charles J, et al. Peripartum Fluoxetine Reduces Maternal Trabecular Bone After Weaning and Elevates Mammary Gland Serotonin and PTHrP. Endocrinology. 2018;159:2850-2862 pubmed publisher
	Zhao X, Xian Y, Wang C, Ding L, Meng X, Zhu W, et al. Calcium-sensing receptor-mediated L-tryptophan-induced secretion of cholecystokinin and glucose-dependent insulinotropic peptide in swine duodenum. J Vet Sci. 2018;19:179-187 pubmed publisher
	Paccou J, Boudot C, Renard C, Liabeuf S, Kamel S, Fardellone P, et al. Total calcium-sensing receptor expression in circulating monocytes is increased in rheumatoid arthritis patients with severe coronary artery calcification. Arthritis Res Ther. 2014;16:412 pubmed publisher
	Zhao W, Gou B, Zhang T, Wang K. Lanthanum chloride bidirectionally influences calcification in bovine vascular smooth muscle cells. J Cell Biochem. 2012;113:1776-86 pubmed publisher
	Wang M, Yao Y, Kuang D, Hampson D. Activation of family C G-protein-coupled receptors by the tripeptide glutathione. J Biol Chem. 2006;281:8864-70 pubmed
	Iemitsu M, Miyauchi T, Maeda S, Tanabe T, Takanashi M, Matsuda M, et al. Exercise training improves cardiac function-related gene levels through thyroid hormone receptor signaling in aged rats. Am J Physiol Heart Circ Physiol. 2004;286:H1696-705 pubmed
	Goldsmith P, Fan G, Ray K, Shiloach J, McPhie P, Rogers K, et al. Expression, purification, and biochemical characterization of the amino-terminal extracellular domain of the human calcium receptor. J Biol Chem. 1999;274:11303-9 pubmed
	Goldsmith P, Fan G, Miller J, Rogers K, Spiegel A. Monoclonal antibodies against synthetic peptides corresponding to the extracellular domain of the human Ca2+ receptor: characterization and use in studying concanavalin A inhibition. J Bone Miner Res. 1997;12:1780-8 pubmed

image

image 1 :

Immunohistochemistry was performed on normal biopsies of deparaffinized Human kidney tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Calcium Sensing Receptor (MA1-934) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

image 2 :

Immunofluorescent analysis of bovine corneal epithelium (CE) limbus tissue extracts using Calcium Sensing Receptor Monoclonal Antibody (Product # MA1-934).

image 3 :

Western blot analysis of calcium sensing receptor in HEK7-2 cell extract using Calcium Sensing Receptor Monoclonal Antibody (Product # MA1-934).

product information

Product Type :

Antibody

Product Name :

Calcium Sensing Receptor Monoclonal Antibody (5C10, ADD)

Catalog # :

MA1-934

Quantity :

100 µg

Price :

US 412.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Bovine, Human, Mouse, Rat

Applications :

ELISA: Assay-dependent, Immunohistochemistry (Frozen): 2 µg/mL, Western Blot: 2 µg/mL

Species :

Bovine, Human, Mouse, Rat

Clone :

5C10, ADD

Isotype :

IgG2a

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Senses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G- protein that activates a phosphatidylinositol-calcium second messenger system.

Immunogen :

Synthetic peptide corresponding to residues (214) A D D D Y G R P G I E K F RE E A E E R D I (235) of human calcium sensing receptor.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunohistochemistry (Frozen): 2 µg/mL, Western Blot: 2 µg/mL

Aliases :

BoPCaR1; Ca sensing receptor; Ca(2+)-sensing receptor; calcium sensing receptor; calcium-sensing receptor; Calcium-sensing receptor (hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism); Calcium-sensing receptor (hypocalciuric hypercalcemia 1, severe neonatal hyperparathyroidism); CaR; CaSR; CaSR antibody; cation sensing receptor; EIG8; Extracellular calcium-sensing receptor; FHH; FIH; G protein coupled receptor, family C, group 2, member A; G protein-coupled receptor, family C, group 2, member A; Gprc2a; HHC; HHC1; HYPOC1; LOW QUALITY PROTEIN: extracellular calcium-sensing receptor; NSHPT; parathyroid Ca(2+)-sensing receptor 1; parathyroid cell calcium-sensing receptor; Parathyroid cell calcium-sensing receptor 1; PCaR1

more info or order :

Invitrogen product webpage