PMCA4 ATPase Monoclonal Antibody (JA9) | Invitrogen MA1-914 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PMCA4 ATPase Monoclonal Antibody (JA9)

catalog :

MA1-914

quantity :

100 µg

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

JA9

reactivity :

human, mouse, rat, bovine, pigs , domestic rabbit

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 20

Published Application/Species/Sample/Dilution	Reference
western blot; human	Ho P, Pang S, Li M, Tse Z, Kung M, Sham P, et al. PMCA4 (ATP2B4) mutation in familial spastic paraplegia causes delay in intracellular calcium extrusion. Brain Behav. 2015;5:e00321 pubmed publisher
immunocytochemistry; mouse; 1:200 western blot; mouse; 1:500	Yang Y, Lee J, Jo H, Park S, Chang I, Muallem S, et al. Homer2 protein regulates plasma membrane Ca²⁺-ATPase-mediated Ca²⁺ signaling in mouse parotid gland acinar cells. J Biol Chem. 2014;289:24971-9 pubmed publisher
immunohistochemistry; bovine; 1:50 western blot; bovine; 1:1000	Schwarz A, Wennemuth G, Post H, Brandenburger T, Aumuller G, Wilhelm B. Vesicular transfer of membrane components to bovine epididymal spermatozoa. Cell Tissue Res. 2013;353:549-61 pubmed publisher
western blot; human; 1:1000	Curry M, Luk N, Kenny P, Roberts Thomson S, Monteith G. Distinct regulation of cytoplasmic calcium signals and cell death pathways by different plasma membrane calcium ATPase isoforms in MDA-MB-231 breast cancer cells. J Biol Chem. 2012;287:28598-608 pubmed publisher
immunohistochemistry; domestic rabbit; 1:1000 western blot; domestic rabbit; 1:2500	Talarico E. Plasma membrane calcium-ATPase isoform four distribution changes during corneal epithelial wound healing. Mol Vis. 2010;16:2259-72 pubmed
immunohistochemistry; human; 1:10	Dally S, Monceau V, Corvazier E, Bredoux R, Raies A, Bobe R, et al. Compartmentalized expression of three novel sarco/endoplasmic reticulum Ca2+ATPase 3 isoforms including the switch to ER stress, SERCA3f, in non-failing and failing human heart. Cell Calcium. 2009;45:144-54 pubmed publisher
immunoprecipitation; mouse; 1 ug/mg immunohistochemistry; mouse; 1:100 western blot; mouse	Baggaley E, McLarnon S, Demeter I, Varga G, Bruce J. Differential regulation of the apical plasma membrane Ca(2+) -ATPase by protein kinase A in parotid acinar cells. J Biol Chem. 2007;282:37678-93 pubmed
immunocytochemistry; rat; 1:300	Xiao J, Gong S, LeDoux M. Caytaxin deficiency disrupts signaling pathways in cerebellar cortex. Neuroscience. 2007;144:439-61 pubmed
immunocytochemistry; human	Weeraratne S, Valentine M, Cusick M, Delay R, Van Houten J. Plasma membrane calcium pumps in mouse olfactory sensory neurons. Chem Senses. 2006;31:725-30 pubmed
	Sritangos P, Pena Alarcon E, James A, Sultan A, Richardson D, Bruce J. Plasma Membrane Ca2+ ATPase Isoform 4 (PMCA4) Has an Important Role in Numerous Hallmarks of Pancreatic Cancer. Cancers (Basel). 2020;12: pubmed publisher
	Lisek M, Ferenc B, Studzian M, Pulaski L, Guo F, Zylinska L, et al. Glutamate Deregulation in Ketamine-Induced Psychosis-A Potential Role of PSD95, NMDA Receptor and PMCA Interaction. Front Cell Neurosci. 2017;11:181 pubmed publisher
	Tauber P, Aichinger B, Christ C, Stindl J, Rhayem Y, Beuschlein F, et al. Cellular Pathophysiology of an Adrenal Adenoma-Associated Mutant of the Plasma Membrane Ca(2+)-ATPase ATP2B3. Endocrinology. 2016;157:2489-99 pubmed publisher
	Kruger W, Yun C, Monteith G, Poronnik P. Muscarinic-induced recruitment of plasma membrane Ca2+-ATPase involves PSD-95/Dlg/Zo-1-mediated interactions. J Biol Chem. 2009;284:1820-30 pubmed publisher
	Sepulveda M, Berrocal Carrillo M, Gasset M, Mata A. The plasma membrane Ca2+-ATPase isoform 4 is localized in lipid rafts of cerebellum synaptic plasma membranes. J Biol Chem. 2006;281:447-53 pubmed
	Talarico E, Kennedy B, Marfurt C, Loeffler K, Mangini N. Expression and immunolocalization of plasma membrane calcium ATPase isoforms in human corneal epithelium. Mol Vis. 2005;11:169-78 pubmed
	Krizaj D, Liu X, Copenhagen D. Expression of calcium transporters in the retina of the tiger salamander (Ambystoma tigrinum). J Comp Neurol. 2004;475:463-80 pubmed
	Tamiya S, Dean W, Paterson C, Delamere N. Regional distribution of Na,K-ATPase activity in porcine lens epithelium. Invest Ophthalmol Vis Sci. 2003;44:4395-9 pubmed
	Filoteo A, Elwess N, Enyedi A, Caride A, Aung H, Penniston J. Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies. J Biol Chem. 1997;272:23741-7 pubmed
	Caride A, Filoteo A, Enyedi A, Verma A, Penniston J. Detection of isoform 4 of the plasma membrane calcium pump in human tissues by using isoform-specific monoclonal antibodies. Biochem J. 1996;316 ( Pt 1):353-9 pubmed
	Adamo H, Caride A, Penniston J. Use of expression mutants and monoclonal antibodies to map the erythrocyte Ca2+ pump. J Biol Chem. 1992;267:14244-9 pubmed

image

image 1 :

Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing PMCA4 ATPase (MA1-914) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

image 2 :

image 3 :

product information

Product Type :

Antibody

Product Name :

PMCA4 ATPase Monoclonal Antibody (JA9)

Catalog # :

MA1-914

Quantity :

100 µg

Price :

US 399.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Bovine, Human, Porcine, Rat

Applications :

ELISA: 1:15,000, Flow Cytometry: 2 µg / 10^6 cells, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Frozen): 1:100-1:500, Immunohistochemistry (Paraffin): 1:100-1:500, Western Blot: 1:1,000

Species :

Bovine, Human, Porcine, Rat

Clone :

JA9

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/K+ pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin (Cat. # MA3-917 and MA3-918).

Immunogen :

Purified human erythrocyte PMCA.

Format :

Liquid

Applications w/Dilutions :

Aliases :

ATP2B2; ATP2B4; ATPase plasma membrane Ca2+ transporting 4; ATPase, Ca++ transporting, plasma membrane 4; ATPase, Ca++ transporting, plasma membrane 4 ATP synthase, H+ transporting, mitochondrial F1; ATPase, Ca++ transporting, plasma membrane 4 tv2; matrix-remodeling-associated protein 1; MXRA1; plasma membrane Ca2+-ATPases 4; plasma membrane calcium ATPase; plasma membrane calcium ATPase 4; plasma membrane calcium ATPase 4 variant b; Plasma membrane calcium ATPase isoform 4; plasma membrane calcium pump; Plasma membrane calcium pump isoform 4; plasma membrane calcium-pumping ATP-ase; plasma membrane calcium-transporting ATPase 4; PMCA4; PMCA4b; PMCA4x; sarcolemmal calcium pump

more info or order :

Invitrogen product webpage