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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
beta Actin Monoclonal Antibody (AC-15)
catalog :
MA1-91399
quantity :
100 µL
price :
US 399.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
AC-15
reactivity :
guinea pig, human, mouse, rat, dogs, chicken, bovine, pigs , zebrafish , cat, domestic sheep, Xenopus laevis, domestic rabbit
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 32
Published Application/Species/Sample/DilutionReference
  • western blot; rat; 1:5000; loading ...; fig 2
Chruvattil R, Banerjee S, Nath S, Machhi J, Kharkwal G, Yadav M, et al. Dexamethasone Alters the Appetite Regulation via Induction of Hypothalamic Insulin Resistance in Rat Brain. Mol Neurobiol. 2017;54:7483-7496 pubmed publisher
  • western blot; human; loading ...; fig 7a
Unsal E, Degirmenci B, Harmanda B, Erman B, Ozlu N. A small molecule identified through an in silico screen inhibits Aurora B-INCENP interaction. Chem Biol Drug Des. 2016;88:783-794 pubmed publisher
  • western blot; mouse; fig 6
Oettinghaus B, D Alonzo D, Barbieri E, Restelli L, Savoia C, Licci M, et al. DRP1-dependent apoptotic mitochondrial fission occurs independently of BAX, BAK and APAF1 to amplify cell death by BID and oxidative stress. Biochim Biophys Acta. 2016;1857:1267-1276 pubmed publisher
  • western blot; human; fig s1
Deisting W, Raum T, Kufer P, Baeuerle P, Münz M. Impact of Diverse Immune Evasion Mechanisms of Cancer Cells on T Cells Engaged by EpCAM/CD3-Bispecific Antibody Construct AMG 110. PLoS ONE. 2015;10:e0141669 pubmed publisher
  • western blot; human; 1:250
Mello A, Leal M, Rey J, Pinto G, Lamarão L, Montenegro R, et al. Deregulated Expression of SRC, LYN and CKB Kinases by DNA Methylation and Its Potential Role in Gastric Cancer Invasiveness and Metastasis. PLoS ONE. 2015;10:e0140492 pubmed publisher
  • western blot; zebrafish ; fig 4
Mukhopadhyay D, Priya P, Chattopadhyay A. Sodium fluoride affects zebrafish behaviour and alters mRNA expressions of biomarker genes in the brain: Role of Nrf2/Keap1. Environ Toxicol Pharmacol. 2015;40:352-9 pubmed publisher
  • western blot; human; 1:8000; fig f3
Badding M, Schwegler Berry D, Park J, Fix N, Cummings K, Leonard S. Sintered indium-tin oxide particles induce pro-inflammatory responses in vitro, in part through inflammasome activation. PLoS ONE. 2015;10:e0124368 pubmed publisher
  • western blot; bovine; 1:5000
Jena M, Janjanam J, Naru J, Kumar S, Kumar S, Singh S, et al. DIGE based proteome analysis of mammary gland tissue in water buffalo (Bubalus bubalis): lactating vis-a-vis heifer. J Proteomics. 2015;119:100-11 pubmed publisher
  • western blot; mouse; 1:5000; fig 4c
Li N, Fassl A, Chick J, Inuzuka H, Li X, Mansour M, et al. Cyclin C is a haploinsufficient tumour suppressor. Nat Cell Biol. 2014;16:1080-91 pubmed publisher
  • western blot; human
Haro K, Sheth A, Scheinberg D. Dysregulation of IRP1-mediated iron metabolism causes gamma ray-specific radioresistance in leukemia cells. PLoS ONE. 2012;7:e48841 pubmed publisher
  • western blot; human; 1:1000; fig 7
Cheung T, Ganatra M, Peters E, Truskey G. Effect of cellular senescence on the albumin permeability of blood-derived endothelial cells. Am J Physiol Heart Circ Physiol. 2012;303:H1374-83 pubmed publisher
  • western blot; human
Toma S, Yamashiro T, Arakaki S, Shiroma J, Maeshiro T, Hibiya K, et al. Inhibition of intracellular hepatitis C virus replication by nelfinavir and synergistic effect with interferon-alpha. J Viral Hepat. 2009;16:506-12 pubmed publisher
Li B, Zhao H, Wu Y, Zhu Y, Zhang J, Yang G, et al. Mitochondrial-Derived Vesicles Protect Cardiomyocytes Against Hypoxic Damage. Front Cell Dev Biol. 2020;8:214 pubmed publisher
Palomo Guerrero M, Fadó R, Casas M, Pérez Montero M, Baena M, Helmer P, et al. Sensing of nutrients by CPT1C regulates late endosome/lysosome anterograde transport and axon growth. elife. 2019;8: pubmed publisher
Tiscione S, Vivas O, Ginsburg K, Bers D, Ory D, Santana L, et al. Disease-associated mutations in Niemann-Pick type C1 alter ER calcium signaling and neuronal plasticity. J Cell Biol. 2019;218:4141-4156 pubmed publisher
Wang X, Long Y, Paucek R, Gooding A, Lee T, Burdorf R, et al. Regulation of histone methylation by automethylation of PRC2. Genes Dev. 2019;33:1416-1427 pubmed publisher
Vivas O, Tiscione S, Dixon R, Ory D, Dickson E. Niemann-Pick Type C Disease Reveals a Link between Lysosomal Cholesterol and PtdIns(4,5)P2 That Regulates Neuronal Excitability. Cell Rep. 2019;27:2636-2648.e4 pubmed publisher
Burat B, Gonzalez J, Sauvage F, Aouad H, Arnion H, Pinault E, et al. Sum of peak intensities outperforms peak area integration in iTRAQ protein expression measurement by LC-MS/MS using a TripleTOF 5600+ platform. Biosci Rep. 2019;: pubmed publisher
González Candia A, Veliz M, Carrasco Pozo C, Castillo R, Cárdenas J, Ebensperger G, et al. Antenatal melatonin modulates an enhanced antioxidant/pro-oxidant ratio in pulmonary hypertensive newborn sheep. Redox Biol. 2019;22:101128 pubmed publisher
Arjunan P, Meghil M, Pi W, Xu J, Lang L, El Awady A, et al. Oral Pathobiont Activates Anti-Apoptotic Pathway, Promoting both Immune Suppression and Oncogenic Cell Proliferation. Sci Rep. 2018;8:16607 pubmed publisher
Astorga C, González Candia A, Candia A, Figueroa E, Cañas D, Ebensperger G, et al. Melatonin Decreases Pulmonary Vascular Remodeling and Oxygen Sensitivity in Pulmonary Hypertensive Newborn Lambs. Front Physiol. 2018;9:185 pubmed publisher
Bajikar S, Wang C, Borten M, Pereira E, Atkins K, Janes K. Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer. Dev Cell. 2017;43:418-435.e13 pubmed publisher
Wozniak D, Kajdacsy Balla A, Macias V, Ball Kell S, Zenner M, Bie W, et al. PTEN is a protein phosphatase that targets active PTK6 and inhibits PTK6 oncogenic signaling in prostate cancer. Nat Commun. 2017;8:1508 pubmed publisher
Wolfram J, Nizzero S, Liu H, Li F, Zhang G, Li Z, et al. A chloroquine-induced macrophage-preconditioning strategy for improved nanodelivery. Sci Rep. 2017;7:13738 pubmed publisher
Caporarello N, Olivieri M, Cristaldi M, Rusciano D, Lupo G, Anfuso C. Melanogenesis in uveal melanoma cells: Effect of argan oil. Int J Mol Med. 2017;40:1277-1284 pubmed publisher
Miosge L, Sontani Y, Chuah A, Horikawa K, Russell T, Mei Y, et al. Systems-guided forward genetic screen reveals a critical role of the replication stress response protein ETAA1 in T cell clonal expansion. Proc Natl Acad Sci U S A. 2017;114:E5216-E5225 pubmed publisher
Pozo M, Rodriguez Rodriguez R, Ramirez S, Seoane Collazo P, Lopez M, Serra D, et al. Hypothalamic Regulation of Liver and Muscle Nutrient Partitioning by Brain-Specific Carnitine Palmitoyltransferase 1C in Male Mice. Endocrinology. 2017;158:2226-2238 pubmed publisher
Sharma S, Wang J, Cortes Gomez E, TAGGART R, Baysal B. Mitochondrial complex II regulates a distinct oxygen sensing mechanism in monocytes. Hum Mol Genet. 2017;26:1328-1339 pubmed publisher
Tyagi R, Miles B, Parmar R, Garg N, Dalai S, Baban B, et al. Human IDO-competent, long-lived immunoregulatory dendritic cells induced by intracellular pathogen, and their fate in humanized mice. Sci Rep. 2017;7:41083 pubmed publisher
Özlü N, Qureshi M, Toyoda Y, Renard B, Mollaoglu G, Özkan N, et al. Quantitative comparison of a human cancer cell surface proteome between interphase and mitosis. EMBO J. 2015;34:251-65 pubmed publisher
Aghdam S, Gurel Z, Ghaffarieh A, Sorenson C, Sheibani N. High glucose and diabetes modulate cellular proteasome function: Implications in the pathogenesis of diabetes complications. Biochem Biophys Res Commun. 2013;432:339-44 pubmed publisher
Xu X, Lee J, Stern D. Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1. J Biol Chem. 2004;279:34091-4 pubmed
product information
Product Type :
Antibody
Product Name :
beta Actin Monoclonal Antibody (AC-15)
Catalog # :
MA1-91399
Quantity :
100 µL
Price :
US 399.00
Clonality :
Monoclonal
Host :
Mouse
Reactivity :
Bovine, Canine, Chicken, Feline, Fish, Guinea Pig, Human, Mouse, Ovine, Porcine, Rabbit, Rat, Xenopus laevis
Applications :
ELISA: Assay-dependent, Immunocytochemistry: 1:1000-1:2000, Immunohistochemistry (Paraffin): Assay-dependent, Western Blot: 1:5,000-1:10,000
Species :
Bovine, Canine, Chicken, Feline, Fish, Guinea Pig, Human, Mouse, Ovine, Porcine, Rabbit, Rat, Xenopus laevis
Clone :
AC-15
Isotype :
IgG1
Storage :
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Description :
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
Immunogen :
synthetic peptide DDDIAALVIDNGSGL conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta).
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-dependent, Immunocytochemistry: 1:1000-1:2000, Immunohistochemistry (Paraffin): Assay-dependent, Western Blot: 1:5,000-1:10,000
Aliases :
0610041G09Rik; AA959943; AAT6; ACT; Act4; Act-4; ACT-5; ACTA; acta1; acta1b; ACTA2; Acta-2; ACTA3; actb; actb.L; actb1; actba; ACTC; actc1; Actc-1; actc1b; ACTE; Actg; ACTG1; Actg2; ACTGE; actin; actin alpha 1; actin alpha 1, skeletal muscle; actin alpha 1, skeletal muscle b; actin alpha 2, smooth muscle; actin alpha cardiac; actin alpha cardiac 1; actin alpha cardiac muscle 1; actin alpha cardiac muscle 1b; actin beta; actin gamma 1; actin gamma 2, smooth muscle; actin, alpha 1, skeletal muscle; actin, alpha 1b, skeletal muscle; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha cardiac muscle 1, intermediate form; actin, alpha cardiac muscle 1b; actin, alpha skeletal muscle; Actin, alpha skeletal muscle, intermediate form; actin, alpha, cardiac 1; actin, alpha, cardiac muscle; actin, alpha, cardiac muscle 1; actin, alpha, vascular smooth muscle; actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; actin, beta; actin, beta 1; actin, beta L homeolog; actin, beta, cytoplasmic; actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; Actin, cytoplasmic 2; Actin, cytoplasmic 2, N-terminally processed; actin, gamma 1; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; actin, gamma-enteric smooth muscle; Actin, gamma-enteric smooth muscle, intermediate form; actin-like protein; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; Actvs; Actx; AL023024; alpha actin 1; alpha-actin cardiac; alpha-actin-1; Alpha-actin-2; alpha-actin-3; alphac-actin; Alpha-cardiac actin; alphaSMA; alpha-smooth muscle actin; ASD5; ASMA; a-SMA; Atcb; Bact; Bact; actin; B-actin; bactin1; bactin1 protein; bactzf; B-ACTZF; beta actin; beta cytoskeletal actin; beta-actin; beta-actin FE-3; beta-actin-1; BRWS1; BRWS2; Cap Z; capZ beta; cardiac muscle alpha actin 1; cardiofunk; cell growth-inhibiting gene 46 protein; Cfk; CFTD; CFTD1; CFTDM; CMD1R; CMH11; cytoplasmic 1; cytoplasmic beta-actin; cytoskeletal beta actin; cytoskeletal gamma-actin; cytoskeletal protein; cytoskeleton actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; DFNA20; DFNA26; E430023M04Rik; E51; epididymis luminal protein 176; fa27h01; fb83f06; gamma actin; gamma non-muscle actin; gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; GIG46; HEL-176; hm:zeh0631; I79_002310; I79_013242; I79_019066; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; nemaline myopathy type 3; PS1TP5-binding protein 1; PS1TP5BP1; SHPM; similar to beta actin; skeletal alpha actin; skeletal alpha1 actin; sma; SMalphaA; SMGA; smooth muscle alpha-actin; smooth muscle gamma-actin; vascular smooth muscle alpha-actin; VSCM; wu:fa27h01; wu:fb63d03; wu:fb83f06; wu:fd18f05; XELAEV_18045052mg; zeh0631
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA