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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
F4/80 Monoclonal Antibody (CI:A3-1)
catalog :
MA1-91124
quantity :
250 ug
price :
US 626.00
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
CI:A3-1
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 51
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; 1:500-1:1000; loading ...; fig s1-2l
Kalinski A, Yoon C, Huffman L, Duncker P, Kohen R, Passino R, et al. Analysis of the immune response to sciatic nerve injury identifies efferocytosis as a key mechanism of nerve debridement. elife. 2020;9: pubmed publisher
  • immunohistochemistry; human; 1:100
Droguett A, Krall P, Burgos M, Valderrama G, Carpio D, Ardiles L, et al. Tubular overexpression of gremlin induces renal damage susceptibility in mice. PLoS ONE. 2014;9:e101879 pubmed publisher
  • flow cytometry; mouse
Sekine C, Koyanagi A, Koyama N, Hozumi K, Chiba S, Yagita H. Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes. Arthritis Res Ther. 2012;14:R45 pubmed publisher
  • flow cytometry; mouse
Hufford M, Kim T, Sun J, Braciale T. Antiviral CD8+ T cell effector activities in situ are regulated by target cell type. J Exp Med. 2011;208:167-80 pubmed publisher
  • flow cytometry; mouse; tbl 1
Thompson J, Chu Y, Glass J, Tapp A, Brown S. The manganese superoxide dismutase mimetic, M40403, protects adult mice from lethal total body irradiation. Free Radic Res. 2010;44:529-40 pubmed publisher
  • flow cytometry; mouse
Thompson J, Chu Y, Glass J, Brown S. Absence of IL-23p19 in donor allogeneic cells reduces mortality from acute GVHD. Bone Marrow Transplant. 2010;45:712-22 pubmed publisher
  • flow cytometry; mouse; fig 1
Dewals B, Hoving J, Leeto M, Marillier R, Govender U, Cutler A, et al. IL-4Ralpha responsiveness of non-CD4 T cells contributes to resistance in schistosoma mansoni infection in pan-T cell-specific IL-4Ralpha-deficient mice. Am J Pathol. 2009;175:706-16 pubmed publisher
  • flow cytometry; mouse
Siegemund S, Hartl A, von Buttlar H, Dautel F, Raue R, Freudenberg M, et al. Conventional bone marrow-derived dendritic cells contribute to toll-like receptor-independent production of alpha/beta interferon in response to inactivated parapoxvirus ovis. J Virol. 2009;83:9411-22 pubmed publisher
  • flow cytometry; mouse; fig 1
Culshaw S, Millington O, Brewer J, McInnes I. Murine neutrophils present Class II restricted antigen. Immunol Lett. 2008;118:49-54 pubmed publisher
  • flow cytometry; mouse
Chen H, ORDOG T, Chen J, YOUNG D, Bardsley M, Redelman D, et al. Differential gene expression in functional classes of interstitial cells of Cajal in murine small intestine. Physiol Genomics. 2007;31:492-509 pubmed
  • flow cytometry; mouse; fig 2
Noel G, Guo X, Wang Q, Schwemberger S, Byrum D, Ogle C. Postburn monocytes are the major producers of TNF-alpha in the heterogeneous splenic macrophage population. Shock. 2007;27:312-9 pubmed
  • flow cytometry; mouse
de Jersey J, Snelgrove S, Palmer S, Teteris S, Mullbacher A, Miller J, et al. Beta cells cannot directly prime diabetogenic CD8 T cells in nonobese diabetic mice. Proc Natl Acad Sci U S A. 2007;104:1295-300 pubmed
  • flow cytometry; mouse
Chen H, Redelman D, Ro S, Ward S, ORDOG T, Sanders K. Selective labeling and isolation of functional classes of interstitial cells of Cajal of human and murine small intestine. Am J Physiol Cell Physiol. 2007;292:C497-507 pubmed
  • flow cytometry; mouse; fig 6
Hewitson J, Jenkins G, Hamblin P, Mountford A. CD40/CD154 interactions are required for the optimal maturation of skin-derived APCs and the induction of helminth-specific IFN-gamma but not IL-4. J Immunol. 2006;177:3209-17 pubmed
  • immunohistochemistry; mouse; 1:100; fig 1
  • western blot; mouse; 1:100; fig 1
Rempel J, Quina L, Blakely Gonzales P, Buchmeier M, Gruol D. Viral induction of central nervous system innate immune responses. J Virol. 2005;79:4369-81 pubmed
  • flow cytometry; mouse; fig 5
Reissinger A, Skinner J, Yuk M. Downregulation of mitogen-activated protein kinases by the Bordetella bronchiseptica Type III secretion system leads to attenuated nonclassical macrophage activation. Infect Immun. 2005;73:308-16 pubmed
  • flow cytometry; mouse; fig 3
Jennings J, Linderman D, Hu B, Sonstein J, Curtis J. Monocytes recruited to the lungs of mice during immune inflammation ingest apoptotic cells poorly. Am J Respir Cell Mol Biol. 2005;32:108-17 pubmed
  • flow cytometry; mouse
Mischenko V, Kapina M, Eruslanov E, Kondratieva E, Lyadova I, Young D, et al. Mycobacterial dissemination and cellular responses after 1-lobe restricted tuberculosis infection of genetically susceptible and resistant mice. J Infect Dis. 2004;190:2137-45 pubmed
  • flow cytometry; mouse; 4 ug/ml; fig 2
ORDOG T, Redelman D, Horváth V, Miller L, Horowitz B, Sanders K. Quantitative analysis by flow cytometry of interstitial cells of Cajal, pacemakers, and mediators of neurotransmission in the gastrointestinal tract. Cytometry A. 2004;62:139-49 pubmed
  • flow cytometry; mouse; fig 4
Mangan N, Fallon R, Smith P, Van Rooijen N, McKenzie A, Fallon P. Helminth infection protects mice from anaphylaxis via IL-10-producing B cells. J Immunol. 2004;173:6346-56 pubmed
  • flow cytometry; mouse
Zheng S, Jiang J, Shen H, Chen Y. Reduced apoptosis and ameliorated listeriosis in TRAIL-null mice. J Immunol. 2004;173:5652-8 pubmed
  • flow cytometry; mouse; fig 4
Mattner J, Wandersee Steinhäuser A, Pahl A, Rollinghoff M, Majeau G, Hochman P, et al. Protection against progressive leishmaniasis by IFN-beta. J Immunol. 2004;172:7574-82 pubmed
  • flow cytometry; mouse; fig 6
Schleicher U, Mattner J, Blos M, Schindler H, Rollinghoff M, Karaghiosoff M, et al. Control of Leishmania major in the absence of Tyk2 kinase. Eur J Immunol. 2004;34:519-29 pubmed
  • flow cytometry; mouse
Eruslanov E, Majorov K, Orlova M, Mischenko V, Kondratieva T, Apt A, et al. Lung cell responses to M. tuberculosis in genetically susceptible and resistant mice following intratracheal challenge. Clin Exp Immunol. 2004;135:19-28 pubmed
  • flow cytometry; mouse; fig 4
Cook A, Braine E, Hamilton J. The phenotype of inflammatory macrophages is stimulus dependent: implications for the nature of the inflammatory response. J Immunol. 2003;171:4816-23 pubmed
  • flow cytometry; mouse
Reading P, Smith G. A kinetic analysis of immune mediators in the lungs of mice infected with vaccinia virus and comparison with intradermal infection. J Gen Virol. 2003;84:1973-83 pubmed
  • flow cytometry; mouse; fig 6, 7
Blos M, Schleicher U, Soares Rocha F, Meissner U, Rollinghoff M, Bogdan C. Organ-specific and stage-dependent control of Leishmania major infection by inducible nitric oxide synthase and phagocyte NADPH oxidase. Eur J Immunol. 2003;33:1224-34 pubmed
  • flow cytometry; mouse
Stavitsky A, Xianli J. In vitro and in vivo regulation by macrophage migration inhibitory factor (MIF) of expression of MHC-II, costimulatory, adhesion, receptor, and cytokine molecules. Cell Immunol. 2002;217:95-104 pubmed
  • flow cytometry; mouse; fig 8
Saio M, Radoja S, Marino M, Frey A. Tumor-infiltrating macrophages induce apoptosis in activated CD8(+) T cells by a mechanism requiring cell contact and mediated by both the cell-associated form of TNF and nitric oxide. J Immunol. 2001;167:5583-93 pubmed
  • flow cytometry; mouse; fig 1
Radoja S, Saio M, Frey A. CD8+ tumor-infiltrating lymphocytes are primed for Fas-mediated activation-induced cell death but are not apoptotic in situ. J Immunol. 2001;166:6074-83 pubmed
Uddin M, Al Hallak M, Khan H, Aboukameel A, Li Y, Bannoura S, et al. Molecular analysis of XPO1 inhibitor and gemcitabine-nab-paclitaxel combination in KPC pancreatic cancer mouse model. Clin Transl Med. 2023;13:e1513 pubmed publisher
Yu D, Zhao J, Lee E, Kim D, Mahapatra R, Rose E, et al. GLUT3 promotes macrophage signaling and function via RAS-mediated endocytosis in atopic dermatitis and wound healing. J Clin Invest. 2023;133: pubmed publisher
Gulen M, Samson N, Keller A, Schwabenland M, Liu C, Gl xfc ck S, et al. cGAS-STING drives ageing-related inflammation and neurodegeneration. Nature. 2023;620:374-380 pubmed publisher
Liu W, Chen W, Xie M, Chen C, Shao Z, Zhang Y, et al. Traumatic brain injury stimulates sympathetic tone-mediated bone marrow myelopoiesis to favor fracture healing. Signal Transduct Target Ther. 2023;8:260 pubmed publisher
Dhillon P, Mulholland K, Hu H, Park J, Sheng X, Abedini A, et al. Increased levels of endogenous retroviruses trigger fibroinflammation and play a role in kidney disease development. Nat Commun. 2023;14:559 pubmed publisher
Gandhirajan A, Roychowdhury S, Kibler C, Cross E, Abraham S, Bellar A, et al. SIRT2-PFKP interaction dysregulates phagocytosis in macrophages with acute ethanol-exposure. Front Immunol. 2022;13:1079962 pubmed publisher
Cribioli E, Giordano Attianese G, Coukos G, Irving M. CAR T cells targeting the ganglioside NGcGM3 control ovarian tumors in the absence of toxicity against healthy tissues. Front Immunol. 2022;13:951143 pubmed publisher
Zhang L, Mamillapalli R, Habata S, McAdow M, Taylor H. Myometrial-derived CXCL12 promotes lipopolysaccharide induced preterm labour by regulating macrophage migration, polarization and function in mice. J Cell Mol Med. 2022;26:2566-2578 pubmed publisher
Noviello C, Kobon K, Delivry L, Guilbert T, Britto F, Julienne F, et al. RhoA within myofibers controls satellite cell microenvironment to allow hypertrophic growth. iScience. 2022;25:103616 pubmed publisher
Fields L, Ito T, Kobayashi K, Ichihara Y, Podaru M, Hussain M, et al. Epicardial placement of human MSC-loaded fibrin sealant films for heart failure: Preclinical efficacy and mechanistic data. Mol Ther. 2021;29:2554-2570 pubmed publisher
Zhang Y, Liu J, Wang X, Zhang J, Xie C. Extracellular vesicle-encapsulated microRNA-23a from dorsal root ganglia neurons binds to A20 and promotes inflammatory macrophage polarization following peripheral nerve injury. Aging (Albany NY). 2021;13:6752-6764 pubmed publisher
Xiong X, Lin Y, Lee J, PAUL A, Yechoor V, FIGUEIRO M, et al. Chronic circadian shift leads to adipose tissue inflammation and fibrosis. Mol Cell Endocrinol. 2021;521:111110 pubmed publisher
Lu Z, Dmitriev I, Brough D, Kashentseva E, Li J, Curiel D. A New Gorilla Adenoviral Vector with Natural Lung Tropism Avoids Liver Toxicity and Is Amenable to Capsid Engineering and Vector Retargeting. J Virol. 2020;94: pubmed publisher
Wang X, de Carvalho Ribeiro M, Iracheta Vellve A, Lowe P, Ambade A, Satishchandran A, et al. Macrophage-Specific Hypoxia-Inducible Factor-1α Contributes to Impaired Autophagic Flux in Nonalcoholic Steatohepatitis. Hepatology. 2018;: pubmed publisher
Vasamsetti S, Florentin J, Coppin E, Stiekema L, Zheng K, Nisar M, et al. Sympathetic Neuronal Activation Triggers Myeloid Progenitor Proliferation and Differentiation. Immunity. 2018;49:93-106.e7 pubmed publisher
Reebye V, Huang K, Lin V, Jarvis S, Cutilas P, Dorman S, et al. Gene activation of CEBPA using saRNA: preclinical studies of the first in human saRNA drug candidate for liver cancer. Oncogene. 2018;37:3216-3228 pubmed publisher
Colinot D, Garbuz T, Bosland M, Wang L, Rice S, Sullivan W, et al. The common parasite Toxoplasma gondii induces prostatic inflammation and microglandular hyperplasia in a mouse model. Prostate. 2017;77:1066-1075 pubmed publisher
Leonhardt F, Grundmann S, Behe M, Bluhm F, Dumont R, Braun F, et al. Inflammatory neovascularization during graft-versus-host disease is regulated by ?v integrin and miR-100. Blood. 2013;121:3307-18 pubmed publisher
Tosello Trampont A, Landes S, Nguyen V, Novobrantseva T, Hahn Y. Kuppfer cells trigger nonalcoholic steatohepatitis development in diet-induced mouse model through tumor necrosis factor-? production. J Biol Chem. 2012;287:40161-72 pubmed publisher
Herold S, Steinmueller M, von Wulffen W, Cakarova L, Pinto R, Pleschka S, et al. Lung epithelial apoptosis in influenza virus pneumonia: the role of macrophage-expressed TNF-related apoptosis-inducing ligand. J Exp Med. 2008;205:3065-77 pubmed publisher
Sangaletti S, Stoppacciaro A, Guiducci C, Torrisi M, Colombo M. Leukocyte, rather than tumor-produced SPARC, determines stroma and collagen type IV deposition in mammary carcinoma. J Exp Med. 2003;198:1475-85 pubmed
product information
Product Type :
Antibody
Product Name :
F4/80 Monoclonal Antibody (CI:A3-1)
Catalog # :
MA1-91124
Quantity :
250 ug
Price :
US 626.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Rat
Reactivity :
Human, Mouse
Applications :
Flow Cytometry: 1:50-1:100, Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): 1:1,000, Immunohistochemistry (Paraffin): 1:50-1:100, Immunomicroscopy: Assay-dependent, Immunoprecipitation: Assay-dependent, Radioimmune assays: Assay-dependent, Western Blot: 10 ug/mL
Species :
Human, Mouse
Clone :
CI:A3-1
Isotype :
IgG2b
Storage :
Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.
Description :
F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
Immunogen :
Thioglycollate stimulated peritoneal macrophages from C57/BL mice
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 1:50-1:100, Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): 1:1,000, Immunohistochemistry (Paraffin): 1:50-1:100, Immunomicroscopy: Assay-dependent, Immunoprecipitation: Assay-dependent, Radioimmune assays: Assay-dependent, Western Blot: 10 ug/mL
Aliases :
ADGRE1; Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA