Chk1 Monoclonal Antibody (DCS-310) | Invitrogen MA1-91087 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Chk1 Monoclonal Antibody (DCS-310)

catalog :

MA1-91087

quantity :

100 ug

price :

US 524.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DCS-310

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human	Sorino C, Bruno T, Desantis A, Di Certo M, Iezzi S, De Nicola F, et al. Centrosomal Che-1 protein is involved in the regulation of mitosis and DNA damage response by mediating pericentrin (PCNT)-dependent Chk1 protein localization. J Biol Chem. 2013;288:23348-57 pubmed publisher
	Collart C, Smith J, Zegerman P. Chk1 Inhibition of the Replication Factor Drf1 Guarantees Cell-Cycle Elongation at the Xenopus laevis Mid-blastula Transition. Dev Cell. 2017;42:82-96.e3 pubmed publisher
	Bekker Jensen S, Lukas C, Kitagawa R, Melander F, Kastan M, Bartek J, et al. Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks. J Cell Biol. 2006;173:195-206 pubmed

product information

Product Type :

Antibody

Product Name :

Chk1 Monoclonal Antibody (DCS-310)

Catalog # :

MA1-91087

Quantity :

100 ug

Price :

US 524.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Immunohistochemistry (Paraffin): 1 ug/mL, Immunoprecipitation: 2 ug / 0.2 mL of cell extract, Western Blot: 1 ug/mL

Species :

Human, Mouse, Rat

Clone :

DCS-310

Isotype :

IgG3

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

CHEK1 (CHK1) is a serine/threonine protein kinase involved in preventing replication when DNA integrity is compromised. CHK1 is a key mediator in the DNA damage-induced checkpoint network. CHK1 is an evolutionarily conserved protein kinase that functions to ensure genomic integrity upon genotoxic stress. When the G2 or S checkpoint is abrogated by the inhibition of CHK1, p53-deficient cancer cells undergo mitotic catastrophe and eventually apoptosis, whereas normal cells are still arrested in the G1 phase. Thus, CHK1 inhibitors can preferentially potentiate the efficacy of DNA damaging agents in cancer cells, and Chk1 is an attractive therapeutic target for cancer treatment, especially since approximately 50% of all human cancers are p53-deficient.

Immunogen :

Recombinant full length protein (Human).

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1 ug/mL, Immunoprecipitation: 2 ug / 0.2 mL of cell extract, Western Blot: 1 ug/mL

Aliases :

C85740; Cell cycle checkpoint kinase; checkpoint kinase 1; checkpoint kinase 1 homolog; checkpoint kinase-1; Checkpoint, S. pombe, homolog of, 1; CHEK1; CHK1; CHK1 checkpoint homolog; Chk1-S; homolog of Chk1-S; HRD1 Protein; rad27; rad27 homolog; serine/threonine protein kinase CHK1; serine/threonine-protein kinase Chk1; Synoviolin; YBR1742; YBR274W

more info or order :

Invitrogen product webpage