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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
AP2 alpha Monoclonal Antibody (3B5)
catalog :
MA1-872
quantity :
100 µL
price :
425 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3B5
reactivity :
human, mouse, chicken
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
citations: 1
image
image 1 :
Immunofluorescent analysis of AP2 using Anti-AP2 Monoclonal Antibody (3B5) (Product# MA1-872) shows staining in MCF-7 Cells. AP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing AP2 (Product# MA1-872) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
image 2 :
Immunofluorescent analysis of AP2 using Anti-AP2 Monoclonal Antibody (3B5) (Product# MA1-872) shows staining in U251 Cells. AP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing AP2 (Product# MA1-872) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
product information
Product Type :
Antibody
Product Name :
AP2 alpha Monoclonal Antibody (3B5)
Catalog # :
MA1-872
Quantity :
100 µL
Price :
425 USD
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Chicken, Human, Mouse
Applications :
Immunocytochemistry: 1:50-1:500, Immunofluorescence: 1:50 - 1:500, Immunohistochemistry: 1:50, Immunoprecipitation: 1:100 - 1:250, Western Blot: 1:50 - 1:200
Species :
Chicken, Human, Mouse
Clone :
3B5
Isotype :
IgG2b
Storage :
-20° C, Avoid Freeze/Thaw Cycles
Description :
TFAP2A is a sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limbs and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. TFAP2A is the only AP-2 protein required for early morphogenesis of the lens vesicle.
Immunogen :
N-terminus of human AP2a.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1:50-1:500, Immunofluorescence: 1:50 - 1:500, Immunohistochemistry: 1:50, Immunoprecipitation: 1:100 - 1:250, Western Blot: 1:50 - 1:200
Aliases :
activating enhancer-binding protein 2 alpha; Activating enhancer-binding protein 2-alpha; Activator protein 2; Ap2; AP-2; Ap-2 (a); AP-2 transcription factor; AP2alpha; AP-2alpha; AP2-alpha; Ap2tf; BOFS; ERF-1; FLJ51761; OTTHUMP00000214240; OTTHUMP00000214243; Tcfap2a; TFAP2; TFAP2A; transcription factor AP-2 alpha; transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha); transcription factor AP-2, alpha; transcription factor AP-2-alpha; transcription factor AP-2-alpha-like
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com81 Wyman Street
Waltham, MA USA 02451
800-678-5599
headquarters: USA
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